Water quality and microbial interaction network topological parameters were the key factors affecting litter decomposition under wetland restoration

Wetland restoration has a significantly impacts biogeochemical cycles. However, the specific mechanisms and potential microbial processes by which wetland restoration influences litter decomposition remain unclear. This knowledge gap hinders our ability to accurately predict ecological processes within wetland ecosystems across various restoration timeframes. Therefore, we conducted an in situ experiment was conducted in wetlands in Northeast China using the litterbag method, involving a restoration time gradient. Natural wetlands served as controls, with wetlands restored for 17 years (R17), 3 years (R3), 2 years (R2), and 1 year (R1). The study focused on two typical wetland plants: reed (Phragmites australis) and sedge (Cyperus rotundus), to investigate the effects of wetland restoration on microbial community, as well as the decomposition of litter. During the experiment, the decomposition rates (k-values) of both types of litter were lower in restored wetlands than natural wetlands. Fungal diversity varied significantly between restored and natural wetlands. In the short term (R3), the community structures of bacteria and fungi in restored wetlands resembled those of natural wetlands. However, significant differences persisted in long-term restored wetlands (R17). Partial Least Squares Path Modeling (PLS-PM) revealed that bacterial network cohesion (network average density, network transitivity) and wetland restoration time are the primary drivers of Reed and Sedge litter decomposition. Overall, wetland restoration enhances litter decomposition by altering the physicochemical properties of water and the characteristics of the microbial interaction network, suggesting that wetland restoration can accelerate the material cycling processes within wetland ecosystems. [Display omitted] •The change of microbial communities with wetland recovery time is nonlinear.•Wetland restoration promotes litter decomposition in northeast China.•Litter decomposition mainly influenced by microbial network topological parameters.