Bovine serum albumin inclusion in the thawing extender improves boar sperm membrane and acrosomal integrity
This study investigates the impact of bovine serum albumin (BSA) inclusion in the thawing extender on boar sperm quality. Thawing protocols in sperm cryopreservation are vital, yet underexplored. It has been determined that BSA can interact with membranes, stabilizing them and preventing damage duri...
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| Veröffentlicht in: | Reproduction in domestic animals 2024-10, Vol.59 (S3), p.e14592-n/a |
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| creator | Álvarez‐Rodríguez, Manuel Nieto‐Cristobal, Helena Mercado, Eduardo |
| description | This study investigates the impact of bovine serum albumin (BSA) inclusion in the thawing extender on boar sperm quality. Thawing protocols in sperm cryopreservation are vital, yet underexplored. It has been determined that BSA can interact with membranes, stabilizing them and preventing damage during the freezing process, for this reason it could also have a beneficial effect during thawing. Our study explores modifications in the conventional Beltsville thawing solution, incorporating BSA at different concentrations (0.005, 0.01, 0.025 and 0.050 g/mL), and evaluating the sperm quality after up to 150 min post‐thawing incubation (37°C). All BSA concentrations preserved plasma membrane and acrosome integrity, relative to control (BSA absence). In addition, 0.025 g/mL BSA group preserves acrosome integrity over time without compromising motility or kinetic parameters. Our study suggests that BSA inclusion in thawing extenders should be considered as an additive for improving post‐thaw boar sperm quality. |
| doi_str_mv | 10.1111/rda.14592 |
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Thawing protocols in sperm cryopreservation are vital, yet underexplored. It has been determined that BSA can interact with membranes, stabilizing them and preventing damage during the freezing process, for this reason it could also have a beneficial effect during thawing. Our study explores modifications in the conventional Beltsville thawing solution, incorporating BSA at different concentrations (0.005, 0.01, 0.025 and 0.050 g/mL), and evaluating the sperm quality after up to 150 min post‐thawing incubation (37°C). All BSA concentrations preserved plasma membrane and acrosome integrity, relative to control (BSA absence). In addition, 0.025 g/mL BSA group preserves acrosome integrity over time without compromising motility or kinetic parameters. Our study suggests that BSA inclusion in thawing extenders should be considered as an additive for improving post‐thaw boar sperm quality.</description><identifier>ISSN: 0936-6768</identifier><identifier>ISSN: 1439-0531</identifier><identifier>EISSN: 1439-0531</identifier><identifier>DOI: 10.1111/rda.14592</identifier><identifier>PMID: 39396862</identifier><language>eng</language><publisher>Germany: Blackwell Publishing Ltd</publisher><subject>Acrosome - drug effects ; additives ; Animals ; boar ; Bovine serum albumin ; Cattle ; Cell Membrane - drug effects ; Cryopreservation ; Cryopreservation - methods ; Cryopreservation - veterinary ; Cryoprotective Agents - pharmacology ; Damage prevention ; extender ; Freezing ; Integrity ; Male ; Melting ; Membranes ; Semen Analysis - veterinary ; Semen Preservation - methods ; Semen Preservation - veterinary ; Serum albumin ; Serum Albumin, Bovine - pharmacology ; Sperm ; Sperm Motility - drug effects ; Spermatozoa - drug effects ; Spermatozoa - physiology ; Swine ; Thawing</subject><ispartof>Reproduction in domestic animals, 2024-10, Vol.59 (S3), p.e14592-n/a</ispartof><rights>2024 The Authors. published by Wiley‐VCH GmbH.</rights><rights>2024 The Authors. Reproduction in Domestic Animals published by Wiley‐VCH GmbH.</rights><rights>2024. This article is published under http://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c2432-b514360344849598d28a260b4a80cf116e5cb387749b0b06bec33969278195b53</cites><orcidid>0000-0003-0321-2431 ; 0000-0003-0120-354X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Frda.14592$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Frda.14592$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39396862$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Álvarez‐Rodríguez, Manuel</creatorcontrib><creatorcontrib>Nieto‐Cristobal, Helena</creatorcontrib><creatorcontrib>Mercado, Eduardo</creatorcontrib><title>Bovine serum albumin inclusion in the thawing extender improves boar sperm membrane and acrosomal integrity</title><title>Reproduction in domestic animals</title><addtitle>Reprod Domest Anim</addtitle><description>This study investigates the impact of bovine serum albumin (BSA) inclusion in the thawing extender on boar sperm quality. Thawing protocols in sperm cryopreservation are vital, yet underexplored. It has been determined that BSA can interact with membranes, stabilizing them and preventing damage during the freezing process, for this reason it could also have a beneficial effect during thawing. Our study explores modifications in the conventional Beltsville thawing solution, incorporating BSA at different concentrations (0.005, 0.01, 0.025 and 0.050 g/mL), and evaluating the sperm quality after up to 150 min post‐thawing incubation (37°C). All BSA concentrations preserved plasma membrane and acrosome integrity, relative to control (BSA absence). In addition, 0.025 g/mL BSA group preserves acrosome integrity over time without compromising motility or kinetic parameters. Our study suggests that BSA inclusion in thawing extenders should be considered as an additive for improving post‐thaw boar sperm quality.</description><subject>Acrosome - drug effects</subject><subject>additives</subject><subject>Animals</subject><subject>boar</subject><subject>Bovine serum albumin</subject><subject>Cattle</subject><subject>Cell Membrane - drug effects</subject><subject>Cryopreservation</subject><subject>Cryopreservation - methods</subject><subject>Cryopreservation - veterinary</subject><subject>Cryoprotective Agents - pharmacology</subject><subject>Damage prevention</subject><subject>extender</subject><subject>Freezing</subject><subject>Integrity</subject><subject>Male</subject><subject>Melting</subject><subject>Membranes</subject><subject>Semen Analysis - veterinary</subject><subject>Semen Preservation - methods</subject><subject>Semen Preservation - veterinary</subject><subject>Serum albumin</subject><subject>Serum Albumin, Bovine - pharmacology</subject><subject>Sperm</subject><subject>Sperm Motility - drug effects</subject><subject>Spermatozoa - drug effects</subject><subject>Spermatozoa - physiology</subject><subject>Swine</subject><subject>Thawing</subject><issn>0936-6768</issn><issn>1439-0531</issn><issn>1439-0531</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><recordid>eNp1kctKxDAUhoMoOl4WvoAE3OiiTtJc2ix1vIIgiK5L0p4Zo007Ju2M8_ZmLroQDBySxcfH-fMjdEzJBY1n6Ct9QblQ6RYaUM5UQgSj22hAFJOJzGS-h_ZDeCeEijzLdtEeU0zJXKYD9HHVzmwDOIDvHda16Z1tsG3Kug-2Xb5w9wZx9Nw2EwxfHTQVeGzd1LczCNi02uMwBe-wA2e8jjLdVFiXvg2t03VUdDDxtlscop2xrgMcbe4D9Hp78zK6Tx6f7h5Gl49JmXKWJkbEDJIwznOuhMqrNNepJIbrnJRjSiWI0rAYhCtDDJEGShbjqDTLqRJGsAN0tvbGFT97CF3hbCihruNubR8KFh2MCZWlET39g763vW_idiuKs-VE6nxNLTMFD-Ni6q3TflFQUiwbKGIDxaqByJ5sjL1xUP2SP18egeEamNsaFv-biufry7XyG6_9jwk</recordid><startdate>202410</startdate><enddate>202410</enddate><creator>Álvarez‐Rodríguez, Manuel</creator><creator>Nieto‐Cristobal, Helena</creator><creator>Mercado, Eduardo</creator><general>Blackwell Publishing Ltd</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-0321-2431</orcidid><orcidid>https://orcid.org/0000-0003-0120-354X</orcidid></search><sort><creationdate>202410</creationdate><title>Bovine serum albumin inclusion in the thawing extender improves boar sperm membrane and acrosomal integrity</title><author>Álvarez‐Rodríguez, Manuel ; Nieto‐Cristobal, Helena ; Mercado, Eduardo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2432-b514360344849598d28a260b4a80cf116e5cb387749b0b06bec33969278195b53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Acrosome - drug effects</topic><topic>additives</topic><topic>Animals</topic><topic>boar</topic><topic>Bovine serum albumin</topic><topic>Cattle</topic><topic>Cell Membrane - drug effects</topic><topic>Cryopreservation</topic><topic>Cryopreservation - methods</topic><topic>Cryopreservation - veterinary</topic><topic>Cryoprotective Agents - pharmacology</topic><topic>Damage prevention</topic><topic>extender</topic><topic>Freezing</topic><topic>Integrity</topic><topic>Male</topic><topic>Melting</topic><topic>Membranes</topic><topic>Semen Analysis - veterinary</topic><topic>Semen Preservation - methods</topic><topic>Semen Preservation - veterinary</topic><topic>Serum albumin</topic><topic>Serum Albumin, Bovine - pharmacology</topic><topic>Sperm</topic><topic>Sperm Motility - drug effects</topic><topic>Spermatozoa - drug effects</topic><topic>Spermatozoa - physiology</topic><topic>Swine</topic><topic>Thawing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Álvarez‐Rodríguez, Manuel</creatorcontrib><creatorcontrib>Nieto‐Cristobal, Helena</creatorcontrib><creatorcontrib>Mercado, Eduardo</creatorcontrib><collection>Wiley Online Library (Open Access Collection)</collection><collection>Wiley Online Library Free Content</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Reproduction in domestic animals</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Álvarez‐Rodríguez, Manuel</au><au>Nieto‐Cristobal, Helena</au><au>Mercado, Eduardo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bovine serum albumin inclusion in the thawing extender improves boar sperm membrane and acrosomal integrity</atitle><jtitle>Reproduction in domestic animals</jtitle><addtitle>Reprod Domest Anim</addtitle><date>2024-10</date><risdate>2024</risdate><volume>59</volume><issue>S3</issue><spage>e14592</spage><epage>n/a</epage><pages>e14592-n/a</pages><issn>0936-6768</issn><issn>1439-0531</issn><eissn>1439-0531</eissn><abstract>This study investigates the impact of bovine serum albumin (BSA) inclusion in the thawing extender on boar sperm quality. Thawing protocols in sperm cryopreservation are vital, yet underexplored. It has been determined that BSA can interact with membranes, stabilizing them and preventing damage during the freezing process, for this reason it could also have a beneficial effect during thawing. Our study explores modifications in the conventional Beltsville thawing solution, incorporating BSA at different concentrations (0.005, 0.01, 0.025 and 0.050 g/mL), and evaluating the sperm quality after up to 150 min post‐thawing incubation (37°C). All BSA concentrations preserved plasma membrane and acrosome integrity, relative to control (BSA absence). In addition, 0.025 g/mL BSA group preserves acrosome integrity over time without compromising motility or kinetic parameters. Our study suggests that BSA inclusion in thawing extenders should be considered as an additive for improving post‐thaw boar sperm quality.</abstract><cop>Germany</cop><pub>Blackwell Publishing Ltd</pub><pmid>39396862</pmid><doi>10.1111/rda.14592</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0003-0321-2431</orcidid><orcidid>https://orcid.org/0000-0003-0120-354X</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Acrosome - drug effects additives Animals boar Bovine serum albumin Cattle Cell Membrane - drug effects Cryopreservation Cryopreservation - methods Cryopreservation - veterinary Cryoprotective Agents - pharmacology Damage prevention extender Freezing Integrity Male Melting Membranes Semen Analysis - veterinary Semen Preservation - methods Semen Preservation - veterinary Serum albumin Serum Albumin, Bovine - pharmacology Sperm Sperm Motility - drug effects Spermatozoa - drug effects Spermatozoa - physiology Swine Thawing |
| title | Bovine serum albumin inclusion in the thawing extender improves boar sperm membrane and acrosomal integrity |
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