NWD1 influences the extension of neuronal axons by regulating microtubule stability
Proteins belonging to the STAND (signal transduction ATPases with numerous domains) family have been implicated in crucial functions across various signal transduction pathways, encompassing both apoptosis and innate immune responses. In this study, we have identified NWD1, a member of the STAND sup...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2024-11, Vol.734, p.150775, Article 150775 |
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| creator | Bao, Tiancheng Yang, Ximan Yu, Jing Li, Mingxuan Guo, Longyu Wang, Qing Bao, Ying Yang, Zhangyi Liu, Yan Guan, Tuchen |
| description | Proteins belonging to the STAND (signal transduction ATPases with numerous domains) family have been implicated in crucial functions across various signal transduction pathways, encompassing both apoptosis and innate immune responses. In this study, we have identified NWD1, a member of the STAND superfamily, as a gene that regulates neurite outgrowth. This was confirmed by siRNA knockdown assay in E18 neurons. A zebrafish model was utilized to create NWD1 knockdown using the NgAgo-gDNA system, revealing the significant role of NWD1 in neurogenesis. We further revealed that NWD1 siRNA reduced the acetylated tubulin protein, and changed the ratio of soluble and polymerized tubulin. Moreover, we investigated the mechanism underlying the regulation of NWD1-mediated microtubule dynamics, and MAP1B may be a target gene. This research unveiled, for the first time, the potential role of NWD1 in regulating axon outgrowth through modulating the ratio of acetylated tubulin.
•NWD1 knockdown suppressed the axonal extension both in vitro and in vivo.•NWD1 regulates the stability of microtubules to affect neurite outgrowth.•MAP1B may be involved in the NWD1-mediated regulation of microtubule dynamics. |
| doi_str_mv | 10.1016/j.bbrc.2024.150775 |
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•NWD1 knockdown suppressed the axonal extension both in vitro and in vivo.•NWD1 regulates the stability of microtubules to affect neurite outgrowth.•MAP1B may be involved in the NWD1-mediated regulation of microtubule dynamics.</description><identifier>ISSN: 0006-291X</identifier><identifier>ISSN: 1090-2104</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2024.150775</identifier><identifier>PMID: 39383832</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Acetylation ; Adenosine Triphosphatases - genetics ; Adenosine Triphosphatases - metabolism ; Animals ; Axons - metabolism ; Cells, Cultured ; Gene Knockdown Techniques ; Humans ; MAP1B ; Microtubule-Associated Proteins - genetics ; Microtubule-Associated Proteins - metabolism ; Microtubules - metabolism ; NACHT and WD repeat domain-containing protein 1 ; Neurite outgrowth ; Neurogenesis ; Neuronal Outgrowth ; Neurons - metabolism ; Tubulin - metabolism ; Tubulin acetylation ; Zebrafish - genetics ; Zebrafish - metabolism ; Zebrafish Proteins - genetics ; Zebrafish Proteins - metabolism</subject><ispartof>Biochemical and biophysical research communications, 2024-11, Vol.734, p.150775, Article 150775</ispartof><rights>2024 Elsevier Inc.</rights><rights>Copyright © 2024 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c237t-376cd9fe577815015f112fa3546c982b49443133633e549a3215e59b68a8f66e3</cites><orcidid>0000-0001-5929-5489 ; 0000-0003-4782-7083</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2024.150775$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39383832$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bao, Tiancheng</creatorcontrib><creatorcontrib>Yang, Ximan</creatorcontrib><creatorcontrib>Yu, Jing</creatorcontrib><creatorcontrib>Li, Mingxuan</creatorcontrib><creatorcontrib>Guo, Longyu</creatorcontrib><creatorcontrib>Wang, Qing</creatorcontrib><creatorcontrib>Bao, Ying</creatorcontrib><creatorcontrib>Yang, Zhangyi</creatorcontrib><creatorcontrib>Liu, Yan</creatorcontrib><creatorcontrib>Guan, Tuchen</creatorcontrib><title>NWD1 influences the extension of neuronal axons by regulating microtubule stability</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Proteins belonging to the STAND (signal transduction ATPases with numerous domains) family have been implicated in crucial functions across various signal transduction pathways, encompassing both apoptosis and innate immune responses. In this study, we have identified NWD1, a member of the STAND superfamily, as a gene that regulates neurite outgrowth. This was confirmed by siRNA knockdown assay in E18 neurons. A zebrafish model was utilized to create NWD1 knockdown using the NgAgo-gDNA system, revealing the significant role of NWD1 in neurogenesis. We further revealed that NWD1 siRNA reduced the acetylated tubulin protein, and changed the ratio of soluble and polymerized tubulin. Moreover, we investigated the mechanism underlying the regulation of NWD1-mediated microtubule dynamics, and MAP1B may be a target gene. This research unveiled, for the first time, the potential role of NWD1 in regulating axon outgrowth through modulating the ratio of acetylated tubulin.
•NWD1 knockdown suppressed the axonal extension both in vitro and in vivo.•NWD1 regulates the stability of microtubules to affect neurite outgrowth.•MAP1B may be involved in the NWD1-mediated regulation of microtubule dynamics.</description><subject>Acetylation</subject><subject>Adenosine Triphosphatases - genetics</subject><subject>Adenosine Triphosphatases - metabolism</subject><subject>Animals</subject><subject>Axons - metabolism</subject><subject>Cells, Cultured</subject><subject>Gene Knockdown Techniques</subject><subject>Humans</subject><subject>MAP1B</subject><subject>Microtubule-Associated Proteins - genetics</subject><subject>Microtubule-Associated Proteins - metabolism</subject><subject>Microtubules - metabolism</subject><subject>NACHT and WD repeat domain-containing protein 1</subject><subject>Neurite outgrowth</subject><subject>Neurogenesis</subject><subject>Neuronal Outgrowth</subject><subject>Neurons - metabolism</subject><subject>Tubulin - metabolism</subject><subject>Tubulin acetylation</subject><subject>Zebrafish - genetics</subject><subject>Zebrafish - metabolism</subject><subject>Zebrafish Proteins - genetics</subject><subject>Zebrafish Proteins - metabolism</subject><issn>0006-291X</issn><issn>1090-2104</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1PGzEQhq0KRMLHH-ih8pHLph5_7VrqBUGhlRAcALU3y-vMpo42XrC9Ffn3bBTaI5rDXN730cxDyGdgC2Cgv64XbZv8gjMuF6BYXatPZA7MsIoDkwdkzhjTFTfwe0aOc14zBiC1OSIzYUQzDZ-Th7tfV0BD7PoRo8dMyx-k-Fow5jBEOnQ04piG6HrqXoeYabulCVdj70qIK7oJPg1lbMceaS6uDX0o21Ny2Lk-49n7PiFP198fL39Ut_c3Py8vbivPRV0qUWu_NB2qum6m80F1ALxzQkntTcNbaaQUIIQWApU0TnBQqEyrG9d0WqM4Ied77nMaXkbMxW5C9tj3LuIwZitgwhoFUkxRvo9O5-acsLPPKWxc2lpgdifTru1Opt3JtHuZU-nLO39sN7j8X_lnbwp82wdw-vJvwGSzDzuNy5DQF7scwkf8N5v2hIo</recordid><startdate>20241119</startdate><enddate>20241119</enddate><creator>Bao, Tiancheng</creator><creator>Yang, Ximan</creator><creator>Yu, Jing</creator><creator>Li, Mingxuan</creator><creator>Guo, Longyu</creator><creator>Wang, Qing</creator><creator>Bao, Ying</creator><creator>Yang, Zhangyi</creator><creator>Liu, Yan</creator><creator>Guan, Tuchen</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-5929-5489</orcidid><orcidid>https://orcid.org/0000-0003-4782-7083</orcidid></search><sort><creationdate>20241119</creationdate><title>NWD1 influences the extension of neuronal axons by regulating microtubule stability</title><author>Bao, Tiancheng ; Yang, Ximan ; Yu, Jing ; Li, Mingxuan ; Guo, Longyu ; Wang, Qing ; Bao, Ying ; Yang, Zhangyi ; Liu, Yan ; Guan, Tuchen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c237t-376cd9fe577815015f112fa3546c982b49443133633e549a3215e59b68a8f66e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Acetylation</topic><topic>Adenosine Triphosphatases - genetics</topic><topic>Adenosine Triphosphatases - metabolism</topic><topic>Animals</topic><topic>Axons - metabolism</topic><topic>Cells, Cultured</topic><topic>Gene Knockdown Techniques</topic><topic>Humans</topic><topic>MAP1B</topic><topic>Microtubule-Associated Proteins - genetics</topic><topic>Microtubule-Associated Proteins - metabolism</topic><topic>Microtubules - metabolism</topic><topic>NACHT and WD repeat domain-containing protein 1</topic><topic>Neurite outgrowth</topic><topic>Neurogenesis</topic><topic>Neuronal Outgrowth</topic><topic>Neurons - metabolism</topic><topic>Tubulin - metabolism</topic><topic>Tubulin acetylation</topic><topic>Zebrafish - genetics</topic><topic>Zebrafish - metabolism</topic><topic>Zebrafish Proteins - genetics</topic><topic>Zebrafish Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bao, Tiancheng</creatorcontrib><creatorcontrib>Yang, Ximan</creatorcontrib><creatorcontrib>Yu, Jing</creatorcontrib><creatorcontrib>Li, Mingxuan</creatorcontrib><creatorcontrib>Guo, Longyu</creatorcontrib><creatorcontrib>Wang, Qing</creatorcontrib><creatorcontrib>Bao, Ying</creatorcontrib><creatorcontrib>Yang, Zhangyi</creatorcontrib><creatorcontrib>Liu, Yan</creatorcontrib><creatorcontrib>Guan, Tuchen</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bao, Tiancheng</au><au>Yang, Ximan</au><au>Yu, Jing</au><au>Li, Mingxuan</au><au>Guo, Longyu</au><au>Wang, Qing</au><au>Bao, Ying</au><au>Yang, Zhangyi</au><au>Liu, Yan</au><au>Guan, Tuchen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>NWD1 influences the extension of neuronal axons by regulating microtubule stability</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2024-11-19</date><risdate>2024</risdate><volume>734</volume><spage>150775</spage><pages>150775-</pages><artnum>150775</artnum><issn>0006-291X</issn><issn>1090-2104</issn><eissn>1090-2104</eissn><abstract>Proteins belonging to the STAND (signal transduction ATPases with numerous domains) family have been implicated in crucial functions across various signal transduction pathways, encompassing both apoptosis and innate immune responses. In this study, we have identified NWD1, a member of the STAND superfamily, as a gene that regulates neurite outgrowth. This was confirmed by siRNA knockdown assay in E18 neurons. A zebrafish model was utilized to create NWD1 knockdown using the NgAgo-gDNA system, revealing the significant role of NWD1 in neurogenesis. We further revealed that NWD1 siRNA reduced the acetylated tubulin protein, and changed the ratio of soluble and polymerized tubulin. Moreover, we investigated the mechanism underlying the regulation of NWD1-mediated microtubule dynamics, and MAP1B may be a target gene. This research unveiled, for the first time, the potential role of NWD1 in regulating axon outgrowth through modulating the ratio of acetylated tubulin.
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| subjects | Acetylation Adenosine Triphosphatases - genetics Adenosine Triphosphatases - metabolism Animals Axons - metabolism Cells, Cultured Gene Knockdown Techniques Humans MAP1B Microtubule-Associated Proteins - genetics Microtubule-Associated Proteins - metabolism Microtubules - metabolism NACHT and WD repeat domain-containing protein 1 Neurite outgrowth Neurogenesis Neuronal Outgrowth Neurons - metabolism Tubulin - metabolism Tubulin acetylation Zebrafish - genetics Zebrafish - metabolism Zebrafish Proteins - genetics Zebrafish Proteins - metabolism |
| title | NWD1 influences the extension of neuronal axons by regulating microtubule stability |
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