Molecular characterization of rabies virus from wild and domestic animals in the Sultanate of Oman
Aims Rabies virus (RV) is endemic in some Arabian countries. However, it is difficult to control RV without understanding the epidemiological evolution of endemic RV isolates. The current study aimed to characterize RV from domestic and wild animal clinical cases in Oman. Methods and Results Twelve...
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| Veröffentlicht in: | Zoonoses and public health 2024-11, Vol.71 (7), p.836-843 |
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| creator | Ali, Haytham Ali, Ahmed Al Mawly, Julanda Tohamy, Hossam G. El‐Neweshy, Mahmoud S. |
| description | Aims
Rabies virus (RV) is endemic in some Arabian countries. However, it is difficult to control RV without understanding the epidemiological evolution of endemic RV isolates. The current study aimed to characterize RV from domestic and wild animal clinical cases in Oman.
Methods and Results
Twelve brain samples from domestic (Five camels, three goats and one cattle) and wild animals (Two foxes and one honey badger) were investigated from different locations in Oman between 2017 and 2020. All samples were confirmed by RV nucleoprotein (N) gene‐specific primers. Seven out of the 12 amplified samples were successfully sequenced and subjected to sequence and phylogenetic analysis. The detected RVs shared an in‐between 96.8%–98.7% and 96.9%–99% nucleotide and amino acid identities, respectively. However, the wild animal RVs shared only 92.6%–93.9% and 95.9% nucleotide and amino acid identities with the domestic animal RVs, respectively. Negri bodies were detected histologically in six brain samples from camels (n = 3), goats (n = 1) and foxes (n = 2). The RVs from domestic animals shared 97%–98.7% and 98%–100% nucleotide and amino acid identities with the previously published fox RVs from Oman and Gulf countries. Phylogenetic analysis suggested that all RV sequences belong to a distinct clade confined to the previously reported clade V within the Middle Eastern Cluster.
Conclusions
As indicated by the analysis of RVs from different locations between 2017 and 2020, a genetic variant isolated to the Gulf region may exist within the Middle East clade. Moreover, it appears that new RV lineages are emerging rapidly within this region. Therefore, a comprehensive genomic and phylogenetic analysis of the circulating RV is important for the development of future prevention and control strategies. |
| doi_str_mv | 10.1111/zph.13164 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3113125932</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3153821591</sourcerecordid><originalsourceid>FETCH-LOGICAL-c2764-c32a6ff05beff9bdbfc1add940f2e42fcd2ea1eeb0a8926749c2e770ccd8ab8e3</originalsourceid><addsrcrecordid>eNqNkUtLJDEUhcMwMt0-FvMHhsBsdNGaR6VSWQ4yPkBpQd24KVLJDZ2mqtKTVDnorzdtty4EwbvJveTL4eYchH5SckxznTyvFseU07L4hqa0KvmMcVl93_ZUCTVBuyktCRFCEfkDTbjipRSSTlFzHVowY6sjNgsdtRkg-mc9-NDj4HDUjYeEH30cE3YxdPi_by3WvcU2dJAGb_LgO90m7Hs8LADfju2gez3A-v280_0-2nH5Hg625x66P_t7d3oxu5qfX57-uZoZJstiZjjTpXNENOCcamzjDNXWqoI4BgVzxjLQFKAhulKslIUyDKQkxthKNxXwPXS40V3F8G_Mu9WdTwbaVvcQxlRzKnjFqFD0C2i2kwnFWUZ_f0CXYYx9_siaYkoKJopMHW0oE0NKEVy9itmV-FRTUq8zqnNG9WtGmf21VRybDuw7-RZKBk42QPYanj5Xqh9uLjaSL9hJnFE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3112975254</pqid></control><display><type>article</type><title>Molecular characterization of rabies virus from wild and domestic animals in the Sultanate of Oman</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Ali, Haytham ; Ali, Ahmed ; Al Mawly, Julanda ; Tohamy, Hossam G. ; El‐Neweshy, Mahmoud S.</creator><creatorcontrib>Ali, Haytham ; Ali, Ahmed ; Al Mawly, Julanda ; Tohamy, Hossam G. ; El‐Neweshy, Mahmoud S.</creatorcontrib><description>Aims
Rabies virus (RV) is endemic in some Arabian countries. However, it is difficult to control RV without understanding the epidemiological evolution of endemic RV isolates. The current study aimed to characterize RV from domestic and wild animal clinical cases in Oman.
Methods and Results
Twelve brain samples from domestic (Five camels, three goats and one cattle) and wild animals (Two foxes and one honey badger) were investigated from different locations in Oman between 2017 and 2020. All samples were confirmed by RV nucleoprotein (N) gene‐specific primers. Seven out of the 12 amplified samples were successfully sequenced and subjected to sequence and phylogenetic analysis. The detected RVs shared an in‐between 96.8%–98.7% and 96.9%–99% nucleotide and amino acid identities, respectively. However, the wild animal RVs shared only 92.6%–93.9% and 95.9% nucleotide and amino acid identities with the domestic animal RVs, respectively. Negri bodies were detected histologically in six brain samples from camels (n = 3), goats (n = 1) and foxes (n = 2). The RVs from domestic animals shared 97%–98.7% and 98%–100% nucleotide and amino acid identities with the previously published fox RVs from Oman and Gulf countries. Phylogenetic analysis suggested that all RV sequences belong to a distinct clade confined to the previously reported clade V within the Middle Eastern Cluster.
Conclusions
As indicated by the analysis of RVs from different locations between 2017 and 2020, a genetic variant isolated to the Gulf region may exist within the Middle East clade. Moreover, it appears that new RV lineages are emerging rapidly within this region. Therefore, a comprehensive genomic and phylogenetic analysis of the circulating RV is important for the development of future prevention and control strategies.</description><identifier>ISSN: 1863-1959</identifier><identifier>ISSN: 1863-2378</identifier><identifier>EISSN: 1863-2378</identifier><identifier>DOI: 10.1111/zph.13164</identifier><identifier>PMID: 39367571</identifier><language>eng</language><publisher>Germany: Blackwell Publishing Ltd</publisher><subject>Amino acid sequence ; Amino acids ; Animals ; Animals, Domestic - virology ; Animals, Wild - virology ; Brain ; Camelidae ; Camelids ; cattle ; Domestic animals ; Epidemiology ; foxes ; Genetic analysis ; Genetic diversity ; Genetic variance ; Genomic analysis ; genomics ; Goats ; Infectious diseases ; Mellivora capensis ; Middle East ; molecular characterization ; Negri bodies ; nucleoproteins ; Nucleotide sequence ; Nucleotides ; Oman ; Oman - epidemiology ; Phylogenetics ; Phylogeny ; Public health ; Rabies ; Rabies - epidemiology ; Rabies - veterinary ; Rabies - virology ; Rabies lyssavirus ; rabies virus ; Rabies virus - classification ; Rabies virus - genetics ; Rabies virus - isolation & purification ; sylvatic rabies ; wild animals ; wildlife ; zoonoses</subject><ispartof>Zoonoses and public health, 2024-11, Vol.71 (7), p.836-843</ispartof><rights>2024 Wiley‐VCH GmbH. Published by John Wiley & Sons Ltd</rights><rights>2024 Wiley‐VCH GmbH. Published by John Wiley & Sons Ltd.</rights><rights>Copyright © 2024 Wiley‐VCH GmbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c2764-c32a6ff05beff9bdbfc1add940f2e42fcd2ea1eeb0a8926749c2e770ccd8ab8e3</cites><orcidid>0000-0002-3273-9593</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fzph.13164$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fzph.13164$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39367571$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ali, Haytham</creatorcontrib><creatorcontrib>Ali, Ahmed</creatorcontrib><creatorcontrib>Al Mawly, Julanda</creatorcontrib><creatorcontrib>Tohamy, Hossam G.</creatorcontrib><creatorcontrib>El‐Neweshy, Mahmoud S.</creatorcontrib><title>Molecular characterization of rabies virus from wild and domestic animals in the Sultanate of Oman</title><title>Zoonoses and public health</title><addtitle>Zoonoses Public Health</addtitle><description>Aims
Rabies virus (RV) is endemic in some Arabian countries. However, it is difficult to control RV without understanding the epidemiological evolution of endemic RV isolates. The current study aimed to characterize RV from domestic and wild animal clinical cases in Oman.
Methods and Results
Twelve brain samples from domestic (Five camels, three goats and one cattle) and wild animals (Two foxes and one honey badger) were investigated from different locations in Oman between 2017 and 2020. All samples were confirmed by RV nucleoprotein (N) gene‐specific primers. Seven out of the 12 amplified samples were successfully sequenced and subjected to sequence and phylogenetic analysis. The detected RVs shared an in‐between 96.8%–98.7% and 96.9%–99% nucleotide and amino acid identities, respectively. However, the wild animal RVs shared only 92.6%–93.9% and 95.9% nucleotide and amino acid identities with the domestic animal RVs, respectively. Negri bodies were detected histologically in six brain samples from camels (n = 3), goats (n = 1) and foxes (n = 2). The RVs from domestic animals shared 97%–98.7% and 98%–100% nucleotide and amino acid identities with the previously published fox RVs from Oman and Gulf countries. Phylogenetic analysis suggested that all RV sequences belong to a distinct clade confined to the previously reported clade V within the Middle Eastern Cluster.
Conclusions
As indicated by the analysis of RVs from different locations between 2017 and 2020, a genetic variant isolated to the Gulf region may exist within the Middle East clade. Moreover, it appears that new RV lineages are emerging rapidly within this region. Therefore, a comprehensive genomic and phylogenetic analysis of the circulating RV is important for the development of future prevention and control strategies.</description><subject>Amino acid sequence</subject><subject>Amino acids</subject><subject>Animals</subject><subject>Animals, Domestic - virology</subject><subject>Animals, Wild - virology</subject><subject>Brain</subject><subject>Camelidae</subject><subject>Camelids</subject><subject>cattle</subject><subject>Domestic animals</subject><subject>Epidemiology</subject><subject>foxes</subject><subject>Genetic analysis</subject><subject>Genetic diversity</subject><subject>Genetic variance</subject><subject>Genomic analysis</subject><subject>genomics</subject><subject>Goats</subject><subject>Infectious diseases</subject><subject>Mellivora capensis</subject><subject>Middle East</subject><subject>molecular characterization</subject><subject>Negri bodies</subject><subject>nucleoproteins</subject><subject>Nucleotide sequence</subject><subject>Nucleotides</subject><subject>Oman</subject><subject>Oman - epidemiology</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Public health</subject><subject>Rabies</subject><subject>Rabies - epidemiology</subject><subject>Rabies - veterinary</subject><subject>Rabies - virology</subject><subject>Rabies lyssavirus</subject><subject>rabies virus</subject><subject>Rabies virus - classification</subject><subject>Rabies virus - genetics</subject><subject>Rabies virus - isolation & purification</subject><subject>sylvatic rabies</subject><subject>wild animals</subject><subject>wildlife</subject><subject>zoonoses</subject><issn>1863-1959</issn><issn>1863-2378</issn><issn>1863-2378</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUtLJDEUhcMwMt0-FvMHhsBsdNGaR6VSWQ4yPkBpQd24KVLJDZ2mqtKTVDnorzdtty4EwbvJveTL4eYchH5SckxznTyvFseU07L4hqa0KvmMcVl93_ZUCTVBuyktCRFCEfkDTbjipRSSTlFzHVowY6sjNgsdtRkg-mc9-NDj4HDUjYeEH30cE3YxdPi_by3WvcU2dJAGb_LgO90m7Hs8LADfju2gez3A-v280_0-2nH5Hg625x66P_t7d3oxu5qfX57-uZoZJstiZjjTpXNENOCcamzjDNXWqoI4BgVzxjLQFKAhulKslIUyDKQkxthKNxXwPXS40V3F8G_Mu9WdTwbaVvcQxlRzKnjFqFD0C2i2kwnFWUZ_f0CXYYx9_siaYkoKJopMHW0oE0NKEVy9itmV-FRTUq8zqnNG9WtGmf21VRybDuw7-RZKBk42QPYanj5Xqh9uLjaSL9hJnFE</recordid><startdate>202411</startdate><enddate>202411</enddate><creator>Ali, Haytham</creator><creator>Ali, Ahmed</creator><creator>Al Mawly, Julanda</creator><creator>Tohamy, Hossam G.</creator><creator>El‐Neweshy, Mahmoud S.</creator><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T2</scope><scope>7U7</scope><scope>7U9</scope><scope>C1K</scope><scope>F1W</scope><scope>H94</scope><scope>H95</scope><scope>K9.</scope><scope>L.G</scope><scope>M7N</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0002-3273-9593</orcidid></search><sort><creationdate>202411</creationdate><title>Molecular characterization of rabies virus from wild and domestic animals in the Sultanate of Oman</title><author>Ali, Haytham ; Ali, Ahmed ; Al Mawly, Julanda ; Tohamy, Hossam G. ; El‐Neweshy, Mahmoud S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2764-c32a6ff05beff9bdbfc1add940f2e42fcd2ea1eeb0a8926749c2e770ccd8ab8e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Amino acid sequence</topic><topic>Amino acids</topic><topic>Animals</topic><topic>Animals, Domestic - virology</topic><topic>Animals, Wild - virology</topic><topic>Brain</topic><topic>Camelidae</topic><topic>Camelids</topic><topic>cattle</topic><topic>Domestic animals</topic><topic>Epidemiology</topic><topic>foxes</topic><topic>Genetic analysis</topic><topic>Genetic diversity</topic><topic>Genetic variance</topic><topic>Genomic analysis</topic><topic>genomics</topic><topic>Goats</topic><topic>Infectious diseases</topic><topic>Mellivora capensis</topic><topic>Middle East</topic><topic>molecular characterization</topic><topic>Negri bodies</topic><topic>nucleoproteins</topic><topic>Nucleotide sequence</topic><topic>Nucleotides</topic><topic>Oman</topic><topic>Oman - epidemiology</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>Public health</topic><topic>Rabies</topic><topic>Rabies - epidemiology</topic><topic>Rabies - veterinary</topic><topic>Rabies - virology</topic><topic>Rabies lyssavirus</topic><topic>rabies virus</topic><topic>Rabies virus - classification</topic><topic>Rabies virus - genetics</topic><topic>Rabies virus - isolation & purification</topic><topic>sylvatic rabies</topic><topic>wild animals</topic><topic>wildlife</topic><topic>zoonoses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ali, Haytham</creatorcontrib><creatorcontrib>Ali, Ahmed</creatorcontrib><creatorcontrib>Al Mawly, Julanda</creatorcontrib><creatorcontrib>Tohamy, Hossam G.</creatorcontrib><creatorcontrib>El‐Neweshy, Mahmoud S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Zoonoses and public health</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ali, Haytham</au><au>Ali, Ahmed</au><au>Al Mawly, Julanda</au><au>Tohamy, Hossam G.</au><au>El‐Neweshy, Mahmoud S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular characterization of rabies virus from wild and domestic animals in the Sultanate of Oman</atitle><jtitle>Zoonoses and public health</jtitle><addtitle>Zoonoses Public Health</addtitle><date>2024-11</date><risdate>2024</risdate><volume>71</volume><issue>7</issue><spage>836</spage><epage>843</epage><pages>836-843</pages><issn>1863-1959</issn><issn>1863-2378</issn><eissn>1863-2378</eissn><abstract>Aims
Rabies virus (RV) is endemic in some Arabian countries. However, it is difficult to control RV without understanding the epidemiological evolution of endemic RV isolates. The current study aimed to characterize RV from domestic and wild animal clinical cases in Oman.
Methods and Results
Twelve brain samples from domestic (Five camels, three goats and one cattle) and wild animals (Two foxes and one honey badger) were investigated from different locations in Oman between 2017 and 2020. All samples were confirmed by RV nucleoprotein (N) gene‐specific primers. Seven out of the 12 amplified samples were successfully sequenced and subjected to sequence and phylogenetic analysis. The detected RVs shared an in‐between 96.8%–98.7% and 96.9%–99% nucleotide and amino acid identities, respectively. However, the wild animal RVs shared only 92.6%–93.9% and 95.9% nucleotide and amino acid identities with the domestic animal RVs, respectively. Negri bodies were detected histologically in six brain samples from camels (n = 3), goats (n = 1) and foxes (n = 2). The RVs from domestic animals shared 97%–98.7% and 98%–100% nucleotide and amino acid identities with the previously published fox RVs from Oman and Gulf countries. Phylogenetic analysis suggested that all RV sequences belong to a distinct clade confined to the previously reported clade V within the Middle Eastern Cluster.
Conclusions
As indicated by the analysis of RVs from different locations between 2017 and 2020, a genetic variant isolated to the Gulf region may exist within the Middle East clade. Moreover, it appears that new RV lineages are emerging rapidly within this region. Therefore, a comprehensive genomic and phylogenetic analysis of the circulating RV is important for the development of future prevention and control strategies.</abstract><cop>Germany</cop><pub>Blackwell Publishing Ltd</pub><pmid>39367571</pmid><doi>10.1111/zph.13164</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-3273-9593</orcidid></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Amino acid sequence Amino acids Animals Animals, Domestic - virology Animals, Wild - virology Brain Camelidae Camelids cattle Domestic animals Epidemiology foxes Genetic analysis Genetic diversity Genetic variance Genomic analysis genomics Goats Infectious diseases Mellivora capensis Middle East molecular characterization Negri bodies nucleoproteins Nucleotide sequence Nucleotides Oman Oman - epidemiology Phylogenetics Phylogeny Public health Rabies Rabies - epidemiology Rabies - veterinary Rabies - virology Rabies lyssavirus rabies virus Rabies virus - classification Rabies virus - genetics Rabies virus - isolation & purification sylvatic rabies wild animals wildlife zoonoses |
| title | Molecular characterization of rabies virus from wild and domestic animals in the Sultanate of Oman |
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