Enzymatic Production of Trehalose and Trehalulose by Immobilized Thermostable Trehalose Synthase
Trehalose, a versatile disaccharide renowned for its unique physical and chemical properties, finds extensive application in the food, pharmaceutical, and cosmetic industries. While conventional extraction methods face challenges, enzymatic conversion offers a promising avenue for the industrial pro...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2024-10, Vol.72 (41), p.22745-22752 |
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| container_issue | 41 |
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| container_title | Journal of agricultural and food chemistry |
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| creator | Yong, Zi-Ling Chen, Yu-Ting Chan, Ching Lee, Guan-Chiun |
| description | Trehalose, a versatile disaccharide renowned for its unique physical and chemical properties, finds extensive application in the food, pharmaceutical, and cosmetic industries. While conventional extraction methods face challenges, enzymatic conversion offers a promising avenue for the industrial production of trehalose. This study delves into a novel synthetic approach utilizing a recombinant enzyme, merging the thermostable trehalose synthase domain from Thermus thermophiles with a cellulose binding domain. Immobilization of this enzyme on cellulose matrices enhances stability and facilitates product purification, opening avenues for efficient enzymatic synthesis. Notably, the engineered enzyme demonstrates additional activity, converting sucrose into trehalulose. This dual functionality, combined with immobilization strategies, holds immense potential for scalable and cost-effective production of trehalose and trehalulose, offering promising prospects in various industrial and biomedical applications. |
| doi_str_mv | 10.1021/acs.jafc.4c07364 |
| format | Article |
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This dual functionality, combined with immobilization strategies, holds immense potential for scalable and cost-effective production of trehalose and trehalulose, offering promising prospects in various industrial and biomedical applications.</description><subject>Biotechnology and Biological Transformations</subject><subject>cellulose</subject><subject>cost effectiveness</subject><subject>domain</subject><subject>enzymes</subject><subject>food chemistry</subject><subject>sucrose</subject><subject>thermal stability</subject><subject>thermophiles</subject><subject>Thermus</subject><subject>trehalose</subject><subject>trehalulose</subject><issn>0021-8561</issn><issn>1520-5118</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqNkc1Lw0AQxRdRbK3ePUmOHkydzXY3yVGKH4WCgvUc92NCU5Js3U0O9a93-6F4ETwNw_zeg3mPkEsKYwoJvZXaj1ey1OOJhpSJyREZUp5AzCnNjskQAhNnXNABOfN-BQAZT-GUDFgeYM6SIXm_bz83jewqHb04a3rdVbaNbBktHC5lbT1GsjWHrd_tahPNmsaqqq4-MZyW6BrrO6lq_KV63bTdUno8JyelrD1eHOaIvD3cL6ZP8fz5cTa9m8cy4UkXT5RBgQYBhEy54UpQAVqyPOUJ1ZmGUpU8M5O0NCkglAyoMXmulEDBwrdsRK73vmtnP3r0XdFUXmNdyxZt7wtGOcsSLhj7B0qTjOcipQGFPaqd9d5hWaxd1Ui3KSgU2wqKUEGxraA4VBAkVwf3XjVofgTfmQfgZg_spLZ3bcjlb78v-IyTUg</recordid><startdate>20241004</startdate><enddate>20241004</enddate><creator>Yong, Zi-Ling</creator><creator>Chen, Yu-Ting</creator><creator>Chan, Ching</creator><creator>Lee, Guan-Chiun</creator><general>American Chemical Society</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0001-9525-6524</orcidid><orcidid>https://orcid.org/0000-0003-1954-0747</orcidid></search><sort><creationdate>20241004</creationdate><title>Enzymatic Production of Trehalose and Trehalulose by Immobilized Thermostable Trehalose Synthase</title><author>Yong, Zi-Ling ; Chen, Yu-Ting ; Chan, Ching ; Lee, Guan-Chiun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a252t-4bde6ede006a75d5b6160ca397521c8c0fbf58d47fd70e0f301dd99bb6e631183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Biotechnology and Biological Transformations</topic><topic>cellulose</topic><topic>cost effectiveness</topic><topic>domain</topic><topic>enzymes</topic><topic>food chemistry</topic><topic>sucrose</topic><topic>thermal stability</topic><topic>thermophiles</topic><topic>Thermus</topic><topic>trehalose</topic><topic>trehalulose</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yong, Zi-Ling</creatorcontrib><creatorcontrib>Chen, Yu-Ting</creatorcontrib><creatorcontrib>Chan, Ching</creatorcontrib><creatorcontrib>Lee, Guan-Chiun</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yong, Zi-Ling</au><au>Chen, Yu-Ting</au><au>Chan, Ching</au><au>Lee, Guan-Chiun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enzymatic Production of Trehalose and Trehalulose by Immobilized Thermostable Trehalose Synthase</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2024-10-04</date><risdate>2024</risdate><volume>72</volume><issue>41</issue><spage>22745</spage><epage>22752</epage><pages>22745-22752</pages><issn>0021-8561</issn><issn>1520-5118</issn><eissn>1520-5118</eissn><abstract>Trehalose, a versatile disaccharide renowned for its unique physical and chemical properties, finds extensive application in the food, pharmaceutical, and cosmetic industries. While conventional extraction methods face challenges, enzymatic conversion offers a promising avenue for the industrial production of trehalose. This study delves into a novel synthetic approach utilizing a recombinant enzyme, merging the thermostable trehalose synthase domain from Thermus thermophiles with a cellulose binding domain. Immobilization of this enzyme on cellulose matrices enhances stability and facilitates product purification, opening avenues for efficient enzymatic synthesis. 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| issn | 0021-8561 1520-5118 1520-5118 |
| language | eng |
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| source | ACS Publications |
| subjects | Biotechnology and Biological Transformations cellulose cost effectiveness domain enzymes food chemistry sucrose thermal stability thermophiles Thermus trehalose trehalulose |
| title | Enzymatic Production of Trehalose and Trehalulose by Immobilized Thermostable Trehalose Synthase |
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