Adlercreutzia wanghongyangiae sp. nov., and Adlercreutzia shanghongiae sp. nov., two new members of the genus Adlercreutzia isolated from plateau pika ( Ochotona curzoniae )

Four anaerobic, Gram-stain-positive, non-motile, non-sporulating rod-shaped bacterial strains (R7 , R21, R22 and R25 ) were isolated from the intestinal contents of plateau pika ( ) collected from the Qinghai-Tibet Plateau, PR China. The four isolates grew at between 25 and 42 °C (optimally at 35-37 °C), and with 0.3-3.3% NaCl (w/v) [optimum, 1.3% (w/v)]. Adding l-arginine to the medium could promote their growth. Strains R7 and R21 were most closely related to B7 (97.48% 16S rRNA gene sequence similarity). Strains R25 and R22 were most closely related to e DSM 19450 (98.25% 16S rRNA gene sequence similarity). The genome sequences of R7 and R25 were 2.89 and 2.90 Mb in size with 63.6 and 62.8 mol% DNA G+C contents, respectively. Phylogenetic analysis based on 16S rRNA gene sequences and core genes revealed that R7 and R21 were most closely related to B7 and DSM 19490 , whereas R25 and R22 were most closely related to DSM 19450 and ResAG-91 . R7 , R25 and the closely related species had average nucleotide identity (ANI) values of 81.9-83.2% as well as digital DNA-DNA hybridisation (dDDH) values between 27.3 and 27.9%, which clearly indicated that they represent two novel species within the genus . For R7 and R25 , meso-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan, and the whole cell sugars included galactose, glucose and ribose. On the basis of these results, we propose that strains R7 and R25 represent two novel species of the genus namely sp. nov. and sp. nov., respectively. The type strains are R7 (=GDMCC 1.4459 =KCTC 25860 ) and R25 (=GDMCC 1.4458 =KCTC 25861 ).