The Rate of Leg Fat Oxidation Is Not Attenuated During Incremental Intensity One‐Leg Knee Extensor Exercise
ABSTRACT It is not clear if fat oxidation is attenuated at higher exercise intensities, when exercising with a small muscle mass, and therefore, we studied leg fat oxidation during graded one‐leg exercise. Ten males (age: 27 ± 2 years, body mass: 82 ± 3 kg, BMI: 24 ± 1 kg m−2, V̇O2max: 49 ± 2 mL min...
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| Veröffentlicht in: | Scandinavian journal of medicine & science in sports 2024-10, Vol.34 (10), p.e14737-n/a |
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| creator | Helge, J. W. Shannon, C. E. Stallknecht, B. Stephens, F. B. Greenhaff, P. L. Dela, F. |
| description | ABSTRACT
It is not clear if fat oxidation is attenuated at higher exercise intensities, when exercising with a small muscle mass, and therefore, we studied leg fat oxidation during graded one‐leg exercise. Ten males (age: 27 ± 2 years, body mass: 82 ± 3 kg, BMI: 24 ± 1 kg m−2, V̇O2max: 49 ± 2 mL min−1 kg−1) performed one‐leg exercise at 25% of maximal workload (Wmax) for 30 min, followed by 120‐min exercise at 55% Wmax with the contralateral leg, and finally 30‐min exercise at 85% Wmax with the first leg. Blood was sampled from an artery and both femoral veins, and blood flow was determined using Doppler ultrasound. Muscle biopsies were obtained before and after 30 min at each workload. One‐way RM ANOVA was applied to determine the impact of exercise intensity. Data are expressed as mean ± SEM. From rest through exercise average blood flow (0.4 ± 0.1, 2.1 ± 0.1, 2.6 ± 0.2, 3.7 ± 0.2 L min−1) and oxygen uptake across the leg (0.03 ± 0.01, 0.23 ± 0.02, 0.35 ± 0.03, 0.53 ± 0.04 L min−1) increased with exercise intensity (p |
| doi_str_mv | 10.1111/sms.14737 |
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It is not clear if fat oxidation is attenuated at higher exercise intensities, when exercising with a small muscle mass, and therefore, we studied leg fat oxidation during graded one‐leg exercise. Ten males (age: 27 ± 2 years, body mass: 82 ± 3 kg, BMI: 24 ± 1 kg m−2, V̇O2max: 49 ± 2 mL min−1 kg−1) performed one‐leg exercise at 25% of maximal workload (Wmax) for 30 min, followed by 120‐min exercise at 55% Wmax with the contralateral leg, and finally 30‐min exercise at 85% Wmax with the first leg. Blood was sampled from an artery and both femoral veins, and blood flow was determined using Doppler ultrasound. Muscle biopsies were obtained before and after 30 min at each workload. One‐way RM ANOVA was applied to determine the impact of exercise intensity. Data are expressed as mean ± SEM. From rest through exercise average blood flow (0.4 ± 0.1, 2.1 ± 0.1, 2.6 ± 0.2, 3.7 ± 0.2 L min−1) and oxygen uptake across the leg (0.03 ± 0.01, 0.23 ± 0.02, 0.35 ± 0.03, 0.53 ± 0.04 L min−1) increased with exercise intensity (p < 0.001). Leg RQ (0.76 ± 0.04, 0.86 ± 0.02,0.87 ± 0.01, 0.92 ± 0.01, p < 0.001), leg plasma FA uptake (2 ± 2, 46 ± 8,83 ± 9, 114 ± 16 μmol min−1; p < 0.001) and rate of leg fat oxidation (0.016 ± 0.005, 0.062 ± 0.012, 0.075 ± 0.011, 0.084 ± 0.018 g min−1, p < 0.007) increased with exercise intensity. Muscle‐free carnitine content was unchanged from rest at 25% Wmax and decreased after 30 min exercise at 55% and 85% Wmax (17.4 ± 1.6, 16.6 ± 0.7, 14.5 ± 1.2, 10.5 ± 1.0 mmol/kg dry muscle, respectively; p < 0.006). During incremental one‐leg exercise, the rate of leg fat oxidation was not attenuated with increasing exercise intensity, probably due to an insufficient muscle metabolic stress response.</description><identifier>ISSN: 0905-7188</identifier><identifier>ISSN: 1600-0838</identifier><identifier>EISSN: 1600-0838</identifier><identifier>DOI: 10.1111/sms.14737</identifier><identifier>PMID: 39350536</identifier><language>eng</language><publisher>Denmark: Blackwell Publishing Ltd</publisher><subject>Adipose Tissue - metabolism ; Adult ; carnitine ; exercise ; Exercise - physiology ; Exercise intensity ; Humans ; Knee - physiology ; Leg - physiology ; Lipid Metabolism - physiology ; Male ; metabolism ; muscle ; Muscle, Skeletal - metabolism ; Muscle, Skeletal - physiology ; one‐leg exercise ; Oxidation ; Oxidation-Reduction ; Oxygen Consumption - physiology ; Regional Blood Flow ; Ultrasonography, Doppler ; Workloads ; Young Adult</subject><ispartof>Scandinavian journal of medicine & science in sports, 2024-10, Vol.34 (10), p.e14737-n/a</ispartof><rights>2024 The Author(s). published by John Wiley & Sons Ltd.</rights><rights>2024 The Author(s). Scandinavian Journal of Medicine & Science In Sports published by John Wiley & Sons Ltd.</rights><rights>2024. This article is published under http://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c2787-bc7935ba7d9aac9b919bfdcdb0210d14dd29078349b6c5bb84bfd39a903f17a83</cites><orcidid>0000-0001-6312-5351 ; 0000-0001-9970-9535 ; 0000-0001-9724-5423</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fsms.14737$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fsms.14737$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39350536$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Helge, J. W.</creatorcontrib><creatorcontrib>Shannon, C. E.</creatorcontrib><creatorcontrib>Stallknecht, B.</creatorcontrib><creatorcontrib>Stephens, F. B.</creatorcontrib><creatorcontrib>Greenhaff, P. L.</creatorcontrib><creatorcontrib>Dela, F.</creatorcontrib><title>The Rate of Leg Fat Oxidation Is Not Attenuated During Incremental Intensity One‐Leg Knee Extensor Exercise</title><title>Scandinavian journal of medicine & science in sports</title><addtitle>Scand J Med Sci Sports</addtitle><description>ABSTRACT
It is not clear if fat oxidation is attenuated at higher exercise intensities, when exercising with a small muscle mass, and therefore, we studied leg fat oxidation during graded one‐leg exercise. Ten males (age: 27 ± 2 years, body mass: 82 ± 3 kg, BMI: 24 ± 1 kg m−2, V̇O2max: 49 ± 2 mL min−1 kg−1) performed one‐leg exercise at 25% of maximal workload (Wmax) for 30 min, followed by 120‐min exercise at 55% Wmax with the contralateral leg, and finally 30‐min exercise at 85% Wmax with the first leg. Blood was sampled from an artery and both femoral veins, and blood flow was determined using Doppler ultrasound. Muscle biopsies were obtained before and after 30 min at each workload. One‐way RM ANOVA was applied to determine the impact of exercise intensity. Data are expressed as mean ± SEM. From rest through exercise average blood flow (0.4 ± 0.1, 2.1 ± 0.1, 2.6 ± 0.2, 3.7 ± 0.2 L min−1) and oxygen uptake across the leg (0.03 ± 0.01, 0.23 ± 0.02, 0.35 ± 0.03, 0.53 ± 0.04 L min−1) increased with exercise intensity (p < 0.001). Leg RQ (0.76 ± 0.04, 0.86 ± 0.02,0.87 ± 0.01, 0.92 ± 0.01, p < 0.001), leg plasma FA uptake (2 ± 2, 46 ± 8,83 ± 9, 114 ± 16 μmol min−1; p < 0.001) and rate of leg fat oxidation (0.016 ± 0.005, 0.062 ± 0.012, 0.075 ± 0.011, 0.084 ± 0.018 g min−1, p < 0.007) increased with exercise intensity. Muscle‐free carnitine content was unchanged from rest at 25% Wmax and decreased after 30 min exercise at 55% and 85% Wmax (17.4 ± 1.6, 16.6 ± 0.7, 14.5 ± 1.2, 10.5 ± 1.0 mmol/kg dry muscle, respectively; p < 0.006). During incremental one‐leg exercise, the rate of leg fat oxidation was not attenuated with increasing exercise intensity, probably due to an insufficient muscle metabolic stress response.</description><subject>Adipose Tissue - metabolism</subject><subject>Adult</subject><subject>carnitine</subject><subject>exercise</subject><subject>Exercise - physiology</subject><subject>Exercise intensity</subject><subject>Humans</subject><subject>Knee - physiology</subject><subject>Leg - physiology</subject><subject>Lipid Metabolism - physiology</subject><subject>Male</subject><subject>metabolism</subject><subject>muscle</subject><subject>Muscle, Skeletal - metabolism</subject><subject>Muscle, Skeletal - physiology</subject><subject>one‐leg exercise</subject><subject>Oxidation</subject><subject>Oxidation-Reduction</subject><subject>Oxygen Consumption - physiology</subject><subject>Regional Blood Flow</subject><subject>Ultrasonography, Doppler</subject><subject>Workloads</subject><subject>Young Adult</subject><issn>0905-7188</issn><issn>1600-0838</issn><issn>1600-0838</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><recordid>eNp1kc1OGzEURi3UCgLtgheoLHUDiyF2PBPbS0QDjQhEKnQ98s8dOtGMh9oekex4BJ6RJ6mHUBZIeOMrfUdHV_dD6JCSE5reOLThhOac8R00olNCMiKY-IRGRJIi41SIPbQfwooQymVe7KI9JllBCjYdofb2D-BfKgLuKryAO3yuIl6ua6ti3Tk8D_i6i_g0RnB9oiz-0fva3eG5Mx5acFE1aU5pqOMGLx08Pz4NmksHgGfrIeh8GsCbOsAX9LlSTYCvr_8B-n0-uz37mS2WF_Oz00VmJlzwTBueFtSKW6mUkVpSqStrrCYTSizNrZ1IwgXLpZ6aQmuRp5hJJQmrKFeCHaCjrffed397CLFs62CgaZSDrg8lS1ebMiHogH5_h6663ru03UBJktOc8kQdbynjuxA8VOW9r1vlNyUl5dBBmTooXzpI7LdXY69bsG_k_6MnYLwFHuoGNh-bypurm63yHxb5kPM</recordid><startdate>202410</startdate><enddate>202410</enddate><creator>Helge, J. W.</creator><creator>Shannon, C. E.</creator><creator>Stallknecht, B.</creator><creator>Stephens, F. B.</creator><creator>Greenhaff, P. L.</creator><creator>Dela, F.</creator><general>Blackwell Publishing Ltd</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TS</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6312-5351</orcidid><orcidid>https://orcid.org/0000-0001-9970-9535</orcidid><orcidid>https://orcid.org/0000-0001-9724-5423</orcidid></search><sort><creationdate>202410</creationdate><title>The Rate of Leg Fat Oxidation Is Not Attenuated During Incremental Intensity One‐Leg Knee Extensor Exercise</title><author>Helge, J. W. ; Shannon, C. E. ; Stallknecht, B. ; Stephens, F. B. ; Greenhaff, P. L. ; Dela, F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2787-bc7935ba7d9aac9b919bfdcdb0210d14dd29078349b6c5bb84bfd39a903f17a83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Adipose Tissue - metabolism</topic><topic>Adult</topic><topic>carnitine</topic><topic>exercise</topic><topic>Exercise - physiology</topic><topic>Exercise intensity</topic><topic>Humans</topic><topic>Knee - physiology</topic><topic>Leg - physiology</topic><topic>Lipid Metabolism - physiology</topic><topic>Male</topic><topic>metabolism</topic><topic>muscle</topic><topic>Muscle, Skeletal - metabolism</topic><topic>Muscle, Skeletal - physiology</topic><topic>one‐leg exercise</topic><topic>Oxidation</topic><topic>Oxidation-Reduction</topic><topic>Oxygen Consumption - physiology</topic><topic>Regional Blood Flow</topic><topic>Ultrasonography, Doppler</topic><topic>Workloads</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Helge, J. W.</creatorcontrib><creatorcontrib>Shannon, C. E.</creatorcontrib><creatorcontrib>Stallknecht, B.</creatorcontrib><creatorcontrib>Stephens, F. B.</creatorcontrib><creatorcontrib>Greenhaff, P. L.</creatorcontrib><creatorcontrib>Dela, F.</creatorcontrib><collection>Wiley Online Library Open Access</collection><collection>Wiley Online Library (Open Access Collection)</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Physical Education Index</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Scandinavian journal of medicine & science in sports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Helge, J. W.</au><au>Shannon, C. 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It is not clear if fat oxidation is attenuated at higher exercise intensities, when exercising with a small muscle mass, and therefore, we studied leg fat oxidation during graded one‐leg exercise. Ten males (age: 27 ± 2 years, body mass: 82 ± 3 kg, BMI: 24 ± 1 kg m−2, V̇O2max: 49 ± 2 mL min−1 kg−1) performed one‐leg exercise at 25% of maximal workload (Wmax) for 30 min, followed by 120‐min exercise at 55% Wmax with the contralateral leg, and finally 30‐min exercise at 85% Wmax with the first leg. Blood was sampled from an artery and both femoral veins, and blood flow was determined using Doppler ultrasound. Muscle biopsies were obtained before and after 30 min at each workload. One‐way RM ANOVA was applied to determine the impact of exercise intensity. Data are expressed as mean ± SEM. From rest through exercise average blood flow (0.4 ± 0.1, 2.1 ± 0.1, 2.6 ± 0.2, 3.7 ± 0.2 L min−1) and oxygen uptake across the leg (0.03 ± 0.01, 0.23 ± 0.02, 0.35 ± 0.03, 0.53 ± 0.04 L min−1) increased with exercise intensity (p < 0.001). Leg RQ (0.76 ± 0.04, 0.86 ± 0.02,0.87 ± 0.01, 0.92 ± 0.01, p < 0.001), leg plasma FA uptake (2 ± 2, 46 ± 8,83 ± 9, 114 ± 16 μmol min−1; p < 0.001) and rate of leg fat oxidation (0.016 ± 0.005, 0.062 ± 0.012, 0.075 ± 0.011, 0.084 ± 0.018 g min−1, p < 0.007) increased with exercise intensity. Muscle‐free carnitine content was unchanged from rest at 25% Wmax and decreased after 30 min exercise at 55% and 85% Wmax (17.4 ± 1.6, 16.6 ± 0.7, 14.5 ± 1.2, 10.5 ± 1.0 mmol/kg dry muscle, respectively; p < 0.006). During incremental one‐leg exercise, the rate of leg fat oxidation was not attenuated with increasing exercise intensity, probably due to an insufficient muscle metabolic stress response.</abstract><cop>Denmark</cop><pub>Blackwell Publishing Ltd</pub><pmid>39350536</pmid><doi>10.1111/sms.14737</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-6312-5351</orcidid><orcidid>https://orcid.org/0000-0001-9970-9535</orcidid><orcidid>https://orcid.org/0000-0001-9724-5423</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Adipose Tissue - metabolism Adult carnitine exercise Exercise - physiology Exercise intensity Humans Knee - physiology Leg - physiology Lipid Metabolism - physiology Male metabolism muscle Muscle, Skeletal - metabolism Muscle, Skeletal - physiology one‐leg exercise Oxidation Oxidation-Reduction Oxygen Consumption - physiology Regional Blood Flow Ultrasonography, Doppler Workloads Young Adult |
| title | The Rate of Leg Fat Oxidation Is Not Attenuated During Incremental Intensity One‐Leg Knee Extensor Exercise |
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