Flow Cytometry Sorting for Random Access in DNA Data Storage: Encapsulation for Enhanced Stability and Sequence Integrity of DNA
As digital data undergo explosive growth, deoxyribonucleic acid (DNA) has emerged as a promising storage medium due to its high density, longevity, and ease of replication, offering vast potential in data storage solutions. This study focuses on the protection and retrieval of data during the DNA st...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2024-10, Vol.96 (40), p.16099-16108 |
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| container_title | Analytical chemistry (Washington) |
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| creator | Zhong, Wukun Geng, Chunyang Fu, Zhangcheng Mao, Cuiping Zheng, Yanlin Wang, Saijie Liu, Kai Yang, Yang Lu, Chunhua Jiang, Xingyu |
| description | As digital data undergo explosive growth, deoxyribonucleic acid (DNA) has emerged as a promising storage medium due to its high density, longevity, and ease of replication, offering vast potential in data storage solutions. This study focuses on the protection and retrieval of data during the DNA storage process, developing a technique that employs flow cytometry sorting (FCS) to segregate multicolored fluorescent DNA microparticles encoded with data and facilitating efficient random access. Moreover, the encapsulated fluorescent DNA microparticles, formed through layer-by-layer self-assembly, preserve structural and sequence integrity even under harsh conditions while also supporting a high-density DNA payload. Experimental results have shown that the encoded data can still be successfully recovered from encapsulated DNA microparticles following de-encapsulation. We also successfully demonstrated the automated encapsulation process of fluorescent DNA microparticles using a microfluidic chip. This research provides an innovative approach to the long-term stability and random readability of DNA data storage. |
| doi_str_mv | 10.1021/acs.analchem.4c04637 |
| format | Article |
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This study focuses on the protection and retrieval of data during the DNA storage process, developing a technique that employs flow cytometry sorting (FCS) to segregate multicolored fluorescent DNA microparticles encoded with data and facilitating efficient random access. Moreover, the encapsulated fluorescent DNA microparticles, formed through layer-by-layer self-assembly, preserve structural and sequence integrity even under harsh conditions while also supporting a high-density DNA payload. Experimental results have shown that the encoded data can still be successfully recovered from encapsulated DNA microparticles following de-encapsulation. We also successfully demonstrated the automated encapsulation process of fluorescent DNA microparticles using a microfluidic chip. This research provides an innovative approach to the long-term stability and random readability of DNA data storage.</description><identifier>ISSN: 0003-2700</identifier><identifier>ISSN: 1520-6882</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/acs.analchem.4c04637</identifier><identifier>PMID: 39319639</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>analytical chemistry ; automation ; Data storage ; Deoxyribonucleic acid ; Digital data ; digital database ; DNA ; DNA - chemistry ; DNA biosynthesis ; Encapsulation ; Flow Cytometry ; Fluorescence ; Fluorescent Dyes - chemistry ; High density ; information storage ; Information Storage and Retrieval ; Integrity ; longevity ; Microfluidics ; Microparticles ; Nucleotide sequence ; organ-on-a-chip ; Random access ; readability ; Self-assembly ; Shelf life ; Stability</subject><ispartof>Analytical chemistry (Washington), 2024-10, Vol.96 (40), p.16099-16108</ispartof><rights>2024 American Chemical Society</rights><rights>Copyright American Chemical Society Oct 8, 2024</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-a288t-6026b50316c1da7a38a6c1093002ba3958f4f20d658a45b7cb7636cd1a55f5cd3</cites><orcidid>0000-0001-7725-885X ; 0000-0003-0878-5191 ; 0000-0002-2630-6062 ; 0000-0002-5008-4703</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.analchem.4c04637$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.analchem.4c04637$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2751,27055,27903,27904,56717,56767</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39319639$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhong, Wukun</creatorcontrib><creatorcontrib>Geng, Chunyang</creatorcontrib><creatorcontrib>Fu, Zhangcheng</creatorcontrib><creatorcontrib>Mao, Cuiping</creatorcontrib><creatorcontrib>Zheng, Yanlin</creatorcontrib><creatorcontrib>Wang, Saijie</creatorcontrib><creatorcontrib>Liu, Kai</creatorcontrib><creatorcontrib>Yang, Yang</creatorcontrib><creatorcontrib>Lu, Chunhua</creatorcontrib><creatorcontrib>Jiang, Xingyu</creatorcontrib><title>Flow Cytometry Sorting for Random Access in DNA Data Storage: Encapsulation for Enhanced Stability and Sequence Integrity of DNA</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>As digital data undergo explosive growth, deoxyribonucleic acid (DNA) has emerged as a promising storage medium due to its high density, longevity, and ease of replication, offering vast potential in data storage solutions. This study focuses on the protection and retrieval of data during the DNA storage process, developing a technique that employs flow cytometry sorting (FCS) to segregate multicolored fluorescent DNA microparticles encoded with data and facilitating efficient random access. Moreover, the encapsulated fluorescent DNA microparticles, formed through layer-by-layer self-assembly, preserve structural and sequence integrity even under harsh conditions while also supporting a high-density DNA payload. Experimental results have shown that the encoded data can still be successfully recovered from encapsulated DNA microparticles following de-encapsulation. We also successfully demonstrated the automated encapsulation process of fluorescent DNA microparticles using a microfluidic chip. This research provides an innovative approach to the long-term stability and random readability of DNA data storage.</description><subject>analytical chemistry</subject><subject>automation</subject><subject>Data storage</subject><subject>Deoxyribonucleic acid</subject><subject>Digital data</subject><subject>digital database</subject><subject>DNA</subject><subject>DNA - chemistry</subject><subject>DNA biosynthesis</subject><subject>Encapsulation</subject><subject>Flow Cytometry</subject><subject>Fluorescence</subject><subject>Fluorescent Dyes - chemistry</subject><subject>High density</subject><subject>information storage</subject><subject>Information Storage and Retrieval</subject><subject>Integrity</subject><subject>longevity</subject><subject>Microfluidics</subject><subject>Microparticles</subject><subject>Nucleotide sequence</subject><subject>organ-on-a-chip</subject><subject>Random access</subject><subject>readability</subject><subject>Self-assembly</subject><subject>Shelf life</subject><subject>Stability</subject><issn>0003-2700</issn><issn>1520-6882</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU2P0zAQhi0EYsvCP0DIEhcuKeP4ow63qtuFlVYgUThHE8fpZpXYxXaEeuOn49DuHjggTmONnvcdSw8hrxksGZTsPZq4RIeDubPjUhgQiq-ekAWTJRRK6_IpWQAAL8oVwAV5EeM9AGPA1HNywSvOKsWrBfl1PfifdHNMfrQpHOnOh9S7Pe18oF_RtX6ka2NsjLR39Orzml5hQrpLPuDefqBbZ_AQpwFT792f0NbdoTO2zQw2_dCnI801dGd_TDbv6Y1Ldh_mte_mwpfkWYdDtK_O85J8v95-23wqbr98vNmsbwsstU6FglI1EjhThrW4Qq4xv6DiAGWDvJK6E10JrZIahWxWplkprkzLUMpOmpZfknen3kPw-Ssx1WMfjR0GdNZPseZMci1As-o_UKgEBy1YRt_-hd77KWQrM8UkE7qSIlPiRJngYwy2qw-hHzEcawb1LLPOMusHmfVZZo69OZdPzWjbx9CDvQzACZjjj4f_2fkb7EmswQ</recordid><startdate>20241008</startdate><enddate>20241008</enddate><creator>Zhong, Wukun</creator><creator>Geng, Chunyang</creator><creator>Fu, Zhangcheng</creator><creator>Mao, Cuiping</creator><creator>Zheng, Yanlin</creator><creator>Wang, Saijie</creator><creator>Liu, Kai</creator><creator>Yang, Yang</creator><creator>Lu, Chunhua</creator><creator>Jiang, Xingyu</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0001-7725-885X</orcidid><orcidid>https://orcid.org/0000-0003-0878-5191</orcidid><orcidid>https://orcid.org/0000-0002-2630-6062</orcidid><orcidid>https://orcid.org/0000-0002-5008-4703</orcidid></search><sort><creationdate>20241008</creationdate><title>Flow Cytometry Sorting for Random Access in DNA Data Storage: Encapsulation for Enhanced Stability and Sequence Integrity of DNA</title><author>Zhong, Wukun ; 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| subjects | analytical chemistry automation Data storage Deoxyribonucleic acid Digital data digital database DNA DNA - chemistry DNA biosynthesis Encapsulation Flow Cytometry Fluorescence Fluorescent Dyes - chemistry High density information storage Information Storage and Retrieval Integrity longevity Microfluidics Microparticles Nucleotide sequence organ-on-a-chip Random access readability Self-assembly Shelf life Stability |
| title | Flow Cytometry Sorting for Random Access in DNA Data Storage: Encapsulation for Enhanced Stability and Sequence Integrity of DNA |
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