Identification of nontuberculous mycobacteria using the GenoType CMdirect and GenoType Mycobacterium CM line probe assays in GeneXpert MTB/RIF Ultra negative sputum: A South African prevalence study
•Prevalence of nontuberculous mycobacteria (NTM) among a GXP-negative cohort was 1.7 %.•CMdirect line probe assay showed a specificity of 100 % as a possible rule-in test.•Culture-independent molecular techniques on decontaminated sputum could aid in NTM identification. The GenoType CMdirect Ver 1.0...
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| Veröffentlicht in: | Diagnostic microbiology and infectious disease 2024-12, Vol.110 (4), p.116531, Article 116531 |
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| container_title | Diagnostic microbiology and infectious disease |
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| creator | Opperman, Christoffel Johannes, Chaldene Singh, Sarishna Ghebrekristos, Yonas Warren, Rob Esmail, Aliasgar Goosen, Wynand |
| description | •Prevalence of nontuberculous mycobacteria (NTM) among a GXP-negative cohort was 1.7 %.•CMdirect line probe assay showed a specificity of 100 % as a possible rule-in test.•Culture-independent molecular techniques on decontaminated sputum could aid in NTM identification.
The GenoType CMdirect Ver 1.0 and GenoType Mycobacterium Common Mycobacteria Ver 2.0-line probe assays were utilized to identify nontuberculous mycobacteria (NTM) from decontaminated sputum in a GeneXpert MTB/RIF Ultra negative cohort. A preliminary NTM prevalence of 1.7 % was observed, with a 100 % specificity. The high specificity advocates the CMdirect as a possible rule-in test for NTM disease. |
| doi_str_mv | 10.1016/j.diagmicrobio.2024.116531 |
| format | Article |
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The GenoType CMdirect Ver 1.0 and GenoType Mycobacterium Common Mycobacteria Ver 2.0-line probe assays were utilized to identify nontuberculous mycobacteria (NTM) from decontaminated sputum in a GeneXpert MTB/RIF Ultra negative cohort. A preliminary NTM prevalence of 1.7 % was observed, with a 100 % specificity. The high specificity advocates the CMdirect as a possible rule-in test for NTM disease.</description><identifier>ISSN: 0732-8893</identifier><identifier>ISSN: 1879-0070</identifier><identifier>EISSN: 1879-0070</identifier><identifier>DOI: 10.1016/j.diagmicrobio.2024.116531</identifier><identifier>PMID: 39293319</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Genotype ; GenoType CMdirect ; GenoType Mycobacterium CM ; Humans ; Molecular assay ; Molecular Diagnostic Techniques - methods ; Mycobacterium Infections, Nontuberculous - diagnosis ; Mycobacterium Infections, Nontuberculous - epidemiology ; Mycobacterium Infections, Nontuberculous - microbiology ; Nontuberculous mycobacteria ; Nontuberculous Mycobacteria - classification ; Nontuberculous Mycobacteria - genetics ; Nontuberculous Mycobacteria - isolation & purification ; Prevalence ; Sensitivity and Specificity ; South Africa ; South Africa - epidemiology ; Sputum ; Sputum - microbiology</subject><ispartof>Diagnostic microbiology and infectious disease, 2024-12, Vol.110 (4), p.116531, Article 116531</ispartof><rights>2024 Elsevier Inc.</rights><rights>Copyright © 2024 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c1689-95a375b7faf8aa61043981b5436e63699d136151e29be3149e6fa391fd6790243</cites><orcidid>0000-0002-6676-5431</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0732889324003560$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39293319$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Opperman, Christoffel</creatorcontrib><creatorcontrib>Johannes, Chaldene</creatorcontrib><creatorcontrib>Singh, Sarishna</creatorcontrib><creatorcontrib>Ghebrekristos, Yonas</creatorcontrib><creatorcontrib>Warren, Rob</creatorcontrib><creatorcontrib>Esmail, Aliasgar</creatorcontrib><creatorcontrib>Goosen, Wynand</creatorcontrib><title>Identification of nontuberculous mycobacteria using the GenoType CMdirect and GenoType Mycobacterium CM line probe assays in GeneXpert MTB/RIF Ultra negative sputum: A South African prevalence study</title><title>Diagnostic microbiology and infectious disease</title><addtitle>Diagn Microbiol Infect Dis</addtitle><description>•Prevalence of nontuberculous mycobacteria (NTM) among a GXP-negative cohort was 1.7 %.•CMdirect line probe assay showed a specificity of 100 % as a possible rule-in test.•Culture-independent molecular techniques on decontaminated sputum could aid in NTM identification.
The GenoType CMdirect Ver 1.0 and GenoType Mycobacterium Common Mycobacteria Ver 2.0-line probe assays were utilized to identify nontuberculous mycobacteria (NTM) from decontaminated sputum in a GeneXpert MTB/RIF Ultra negative cohort. A preliminary NTM prevalence of 1.7 % was observed, with a 100 % specificity. The high specificity advocates the CMdirect as a possible rule-in test for NTM disease.</description><subject>Genotype</subject><subject>GenoType CMdirect</subject><subject>GenoType Mycobacterium CM</subject><subject>Humans</subject><subject>Molecular assay</subject><subject>Molecular Diagnostic Techniques - methods</subject><subject>Mycobacterium Infections, Nontuberculous - diagnosis</subject><subject>Mycobacterium Infections, Nontuberculous - epidemiology</subject><subject>Mycobacterium Infections, Nontuberculous - microbiology</subject><subject>Nontuberculous mycobacteria</subject><subject>Nontuberculous Mycobacteria - classification</subject><subject>Nontuberculous Mycobacteria - genetics</subject><subject>Nontuberculous Mycobacteria - isolation & purification</subject><subject>Prevalence</subject><subject>Sensitivity and Specificity</subject><subject>South Africa</subject><subject>South Africa - epidemiology</subject><subject>Sputum</subject><subject>Sputum - microbiology</subject><issn>0732-8893</issn><issn>1879-0070</issn><issn>1879-0070</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkV1v0zAUhiMEYmXwF5DFFTfpfOLEiXdXOjYqrUKCTuLOcpKTzlXiFH9Uyh_kd-Eqg3HJlSXrOV_vkyQfgC6BAr86LFut9oNu7FjrcZnRLF8C8ILBi2QBVSlSSkv6MlnQkmVpVQl2kbxx7kApZCKnr5MLJjLBGIhF8mvTovG6043yejRk7IgZjQ812ib0Y3BkmJqxVo1HqxUJTps98Y9I7tCMu-mIZL1ttcXGE2Xa59_tc1UYIkN6bZAc48ZIlHNqckSbM44_jmg92e4-XX3b3JKH3ltFDO7jOick7hh8GK7Jinwfg38kq87GTU1shCfVo2ki4kM7vU1edap3-O7pvUwebj_v1l_S-693m_XqPm2AVyIVhWJlUZed6iqlONCciQrqImccOeNCtMA4FICZqJFBLpB3ignoWl6KGDO7TD7OfeMlPwM6LwftGux7ZTCGJRlQHkNnABG9ntGoyTmLnTxaPSg7SaDy7FEe5L8e5dmjnD3G4vdPc0I9YPu39I-4CNzMAMZrTxqtdI0-5zHLkO2o_2fOb7GiuEM</recordid><startdate>202412</startdate><enddate>202412</enddate><creator>Opperman, Christoffel</creator><creator>Johannes, Chaldene</creator><creator>Singh, Sarishna</creator><creator>Ghebrekristos, Yonas</creator><creator>Warren, Rob</creator><creator>Esmail, Aliasgar</creator><creator>Goosen, Wynand</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-6676-5431</orcidid></search><sort><creationdate>202412</creationdate><title>Identification of nontuberculous mycobacteria using the GenoType CMdirect and GenoType Mycobacterium CM line probe assays in GeneXpert MTB/RIF Ultra negative sputum: A South African prevalence study</title><author>Opperman, Christoffel ; 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The GenoType CMdirect Ver 1.0 and GenoType Mycobacterium Common Mycobacteria Ver 2.0-line probe assays were utilized to identify nontuberculous mycobacteria (NTM) from decontaminated sputum in a GeneXpert MTB/RIF Ultra negative cohort. A preliminary NTM prevalence of 1.7 % was observed, with a 100 % specificity. The high specificity advocates the CMdirect as a possible rule-in test for NTM disease.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>39293319</pmid><doi>10.1016/j.diagmicrobio.2024.116531</doi><orcidid>https://orcid.org/0000-0002-6676-5431</orcidid></addata></record> |
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| subjects | Genotype GenoType CMdirect GenoType Mycobacterium CM Humans Molecular assay Molecular Diagnostic Techniques - methods Mycobacterium Infections, Nontuberculous - diagnosis Mycobacterium Infections, Nontuberculous - epidemiology Mycobacterium Infections, Nontuberculous - microbiology Nontuberculous mycobacteria Nontuberculous Mycobacteria - classification Nontuberculous Mycobacteria - genetics Nontuberculous Mycobacteria - isolation & purification Prevalence Sensitivity and Specificity South Africa South Africa - epidemiology Sputum Sputum - microbiology |
| title | Identification of nontuberculous mycobacteria using the GenoType CMdirect and GenoType Mycobacterium CM line probe assays in GeneXpert MTB/RIF Ultra negative sputum: A South African prevalence study |
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