Isolation and Characterization of Extended-Spectrum β-Lactamase Producing Escherichia coli from Pig Farms and Slaughterhouse
Extended-spectrum β-lactamase (ESBL) producing Escherichia coli represents a formidable challenge in the field of microbiology and public health due to its resistance to commonly used antibiotics. These strains pose a serious threat to human and animal health, underscoring the urgency of comprehensi...
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| Veröffentlicht in: | Indian journal of microbiology 2024-09, Vol.64 (3), p.950-956 |
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| creator | Niharika, Jagana Deb, Rajib Parihar, Ranjeet Thakur, Priyanka Kumari Anjaria, Pranav Sengar, Gyanendra Singh Chaudhary, Parul Pegu, Seema Rani Attupurum, Nitin Antony, Naveena Rajkhowa, Swaraj Gupta, Vivek Kumar |
| description | Extended-spectrum β-lactamase (ESBL) producing
Escherichia coli
represents a formidable challenge in the field of microbiology and public health due to its resistance to commonly used antibiotics. These strains pose a serious threat to human and animal health, underscoring the urgency of comprehensive research and surveillance. The ongoing investigation seeks ESBL producing
E. coli
strains from pig farms and slaughterhouses in West Bengal and Assam, India. A total of 309 samples were collected: nasal swabs (25), rectal swabs (25) from healthy pigs, pig pen soil (45), faeces (55), slaughterhouse effluents (115), and cleaning water (44). In these samples, 154 tested positive for
E. coli
, indicating a 49.8% prevalence. Among 154
E. coli
isolates, 23 (14.9%) produced ESBLs, sourced from pig rectal swabs (7.1%), faeces (10.7%), slaughterhouse effluents (26.1%), and cleaning water (11.7%). Significantly, 4 ESBL
E. coli
isolates (6.6%) exclusively emerged from pig slaughterhouse effluents, displaying imipenem-resistant properties. The majority of ESBL
E. coli
primarily produced CTX-M and CMY, with consistent genetic markers
bla
CTX-M (100%) and
bla
CMY (82.6%). Remarkably, 2 (8.6%) of 17 ESBL
E. coli
isolates from pig slaughterhouse effluents carried the genetic marker
bla
NDM1. These findings stress implementing thorough surveillance in pig farms and local slaughterhouses. This proactive approach is crucial to identify ESBL
E. coli
strains, enhancing public health protection. |
| doi_str_mv | 10.1007/s12088-023-01151-z |
| format | Article |
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Escherichia coli
represents a formidable challenge in the field of microbiology and public health due to its resistance to commonly used antibiotics. These strains pose a serious threat to human and animal health, underscoring the urgency of comprehensive research and surveillance. The ongoing investigation seeks ESBL producing
E. coli
strains from pig farms and slaughterhouses in West Bengal and Assam, India. A total of 309 samples were collected: nasal swabs (25), rectal swabs (25) from healthy pigs, pig pen soil (45), faeces (55), slaughterhouse effluents (115), and cleaning water (44). In these samples, 154 tested positive for
E. coli
, indicating a 49.8% prevalence. Among 154
E. coli
isolates, 23 (14.9%) produced ESBLs, sourced from pig rectal swabs (7.1%), faeces (10.7%), slaughterhouse effluents (26.1%), and cleaning water (11.7%). Significantly, 4 ESBL
E. coli
isolates (6.6%) exclusively emerged from pig slaughterhouse effluents, displaying imipenem-resistant properties. The majority of ESBL
E. coli
primarily produced CTX-M and CMY, with consistent genetic markers
bla
CTX-M (100%) and
bla
CMY (82.6%). Remarkably, 2 (8.6%) of 17 ESBL
E. coli
isolates from pig slaughterhouse effluents carried the genetic marker
bla
NDM1. These findings stress implementing thorough surveillance in pig farms and local slaughterhouses. This proactive approach is crucial to identify ESBL
E. coli
strains, enhancing public health protection.</description><identifier>ISSN: 0046-8991</identifier><identifier>EISSN: 0973-7715</identifier><identifier>DOI: 10.1007/s12088-023-01151-z</identifier><identifier>PMID: 39282198</identifier><language>eng</language><publisher>New Delhi: Springer India</publisher><subject>Abattoirs ; Animal health ; Antibiotics ; Biomedical and Life Sciences ; Cleaning ; E coli ; Effluents ; Escherichia coli ; Farms ; Feces ; Genetic markers ; health promotion ; Imipenem ; India ; Life Sciences ; Medical Microbiology ; Microbiology ; monitoring ; nose ; Original Research Article ; Public health ; Rectum ; slaughterhouses ; soil ; Soil water ; Surveillance ; Swine ; β Lactamase</subject><ispartof>Indian journal of microbiology, 2024-09, Vol.64 (3), p.950-956</ispartof><rights>Association of Microbiologists of India 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-e8f5ee27c35f15dd5ea46bf423bd269e9f8768fa130750d0c07fb861db2ba1c73</citedby><cites>FETCH-LOGICAL-c408t-e8f5ee27c35f15dd5ea46bf423bd269e9f8768fa130750d0c07fb861db2ba1c73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12088-023-01151-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12088-023-01151-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39282198$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Niharika, Jagana</creatorcontrib><creatorcontrib>Deb, Rajib</creatorcontrib><creatorcontrib>Parihar, Ranjeet</creatorcontrib><creatorcontrib>Thakur, Priyanka Kumari</creatorcontrib><creatorcontrib>Anjaria, Pranav</creatorcontrib><creatorcontrib>Sengar, Gyanendra Singh</creatorcontrib><creatorcontrib>Chaudhary, Parul</creatorcontrib><creatorcontrib>Pegu, Seema Rani</creatorcontrib><creatorcontrib>Attupurum, Nitin</creatorcontrib><creatorcontrib>Antony, Naveena</creatorcontrib><creatorcontrib>Rajkhowa, Swaraj</creatorcontrib><creatorcontrib>Gupta, Vivek Kumar</creatorcontrib><title>Isolation and Characterization of Extended-Spectrum β-Lactamase Producing Escherichia coli from Pig Farms and Slaughterhouse</title><title>Indian journal of microbiology</title><addtitle>Indian J Microbiol</addtitle><addtitle>Indian J Microbiol</addtitle><description>Extended-spectrum β-lactamase (ESBL) producing
Escherichia coli
represents a formidable challenge in the field of microbiology and public health due to its resistance to commonly used antibiotics. These strains pose a serious threat to human and animal health, underscoring the urgency of comprehensive research and surveillance. The ongoing investigation seeks ESBL producing
E. coli
strains from pig farms and slaughterhouses in West Bengal and Assam, India. A total of 309 samples were collected: nasal swabs (25), rectal swabs (25) from healthy pigs, pig pen soil (45), faeces (55), slaughterhouse effluents (115), and cleaning water (44). In these samples, 154 tested positive for
E. coli
, indicating a 49.8% prevalence. Among 154
E. coli
isolates, 23 (14.9%) produced ESBLs, sourced from pig rectal swabs (7.1%), faeces (10.7%), slaughterhouse effluents (26.1%), and cleaning water (11.7%). Significantly, 4 ESBL
E. coli
isolates (6.6%) exclusively emerged from pig slaughterhouse effluents, displaying imipenem-resistant properties. The majority of ESBL
E. coli
primarily produced CTX-M and CMY, with consistent genetic markers
bla
CTX-M (100%) and
bla
CMY (82.6%). Remarkably, 2 (8.6%) of 17 ESBL
E. coli
isolates from pig slaughterhouse effluents carried the genetic marker
bla
NDM1. These findings stress implementing thorough surveillance in pig farms and local slaughterhouses. This proactive approach is crucial to identify ESBL
E. coli
strains, enhancing public health protection.</description><subject>Abattoirs</subject><subject>Animal health</subject><subject>Antibiotics</subject><subject>Biomedical and Life Sciences</subject><subject>Cleaning</subject><subject>E coli</subject><subject>Effluents</subject><subject>Escherichia coli</subject><subject>Farms</subject><subject>Feces</subject><subject>Genetic markers</subject><subject>health promotion</subject><subject>Imipenem</subject><subject>India</subject><subject>Life Sciences</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>monitoring</subject><subject>nose</subject><subject>Original Research Article</subject><subject>Public health</subject><subject>Rectum</subject><subject>slaughterhouses</subject><subject>soil</subject><subject>Soil water</subject><subject>Surveillance</subject><subject>Swine</subject><subject>β Lactamase</subject><issn>0046-8991</issn><issn>0973-7715</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqNkc9qFTEYxYMotlZfwIUE3LiJfkkmk2Qpl9tauGChuh4y-XNnyszkmsyAXvClfBCfqWmnKrgQV_lIfuecfByEXlJ4SwHku0wZKEWAcQKUCkqOj9ApaMmJlFQ8LjNUNVFa0xP0LOcbAFHrWjxFJ1wzxahWp-j7ZY6Dmfs4YTM5vOlMMnb2qT-ulzHg7dfZT847cn3wdk7LiH_-ILtCmdFkj69SdIvtpz3eZtsVpe16g20cehxSHPFVv8fnJo35PuB6MMu-KwFdXLJ_jp4EM2T_4uE8Q5_Pt582H8ju48Xl5v2O2ArUTLwKwnsmLReBCueEN1Xdhorx1rFaex2UrFUwlIMU4MCCDK2qqWtZa6iV_Ay9WX0PKX5ZfJ6bsc_WD4OZfPlHw6ngUnOg6j9QqIErUYuCvv4LvYlLmsoid1RVSaE1LxRbKZtizsmH5pD60aRvDYXmrsdm7bEpPTb3PTbHInr1YL20o3e_Jb-KKwBfgVyepr1Pf7L_YXsLQdyq1A</recordid><startdate>20240901</startdate><enddate>20240901</enddate><creator>Niharika, Jagana</creator><creator>Deb, Rajib</creator><creator>Parihar, Ranjeet</creator><creator>Thakur, Priyanka Kumari</creator><creator>Anjaria, Pranav</creator><creator>Sengar, Gyanendra Singh</creator><creator>Chaudhary, Parul</creator><creator>Pegu, Seema Rani</creator><creator>Attupurum, Nitin</creator><creator>Antony, Naveena</creator><creator>Rajkhowa, Swaraj</creator><creator>Gupta, Vivek Kumar</creator><general>Springer India</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20240901</creationdate><title>Isolation and Characterization of Extended-Spectrum β-Lactamase Producing Escherichia coli from Pig Farms and Slaughterhouse</title><author>Niharika, Jagana ; Deb, Rajib ; Parihar, Ranjeet ; Thakur, Priyanka Kumari ; Anjaria, Pranav ; Sengar, Gyanendra Singh ; Chaudhary, Parul ; Pegu, Seema Rani ; Attupurum, Nitin ; Antony, Naveena ; Rajkhowa, Swaraj ; Gupta, Vivek Kumar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-e8f5ee27c35f15dd5ea46bf423bd269e9f8768fa130750d0c07fb861db2ba1c73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Abattoirs</topic><topic>Animal health</topic><topic>Antibiotics</topic><topic>Biomedical and Life Sciences</topic><topic>Cleaning</topic><topic>E coli</topic><topic>Effluents</topic><topic>Escherichia coli</topic><topic>Farms</topic><topic>Feces</topic><topic>Genetic markers</topic><topic>health promotion</topic><topic>Imipenem</topic><topic>India</topic><topic>Life Sciences</topic><topic>Medical Microbiology</topic><topic>Microbiology</topic><topic>monitoring</topic><topic>nose</topic><topic>Original Research Article</topic><topic>Public health</topic><topic>Rectum</topic><topic>slaughterhouses</topic><topic>soil</topic><topic>Soil water</topic><topic>Surveillance</topic><topic>Swine</topic><topic>β Lactamase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Niharika, Jagana</creatorcontrib><creatorcontrib>Deb, Rajib</creatorcontrib><creatorcontrib>Parihar, Ranjeet</creatorcontrib><creatorcontrib>Thakur, Priyanka Kumari</creatorcontrib><creatorcontrib>Anjaria, Pranav</creatorcontrib><creatorcontrib>Sengar, Gyanendra Singh</creatorcontrib><creatorcontrib>Chaudhary, Parul</creatorcontrib><creatorcontrib>Pegu, Seema Rani</creatorcontrib><creatorcontrib>Attupurum, Nitin</creatorcontrib><creatorcontrib>Antony, Naveena</creatorcontrib><creatorcontrib>Rajkhowa, Swaraj</creatorcontrib><creatorcontrib>Gupta, Vivek Kumar</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Indian journal of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Niharika, Jagana</au><au>Deb, Rajib</au><au>Parihar, Ranjeet</au><au>Thakur, Priyanka Kumari</au><au>Anjaria, Pranav</au><au>Sengar, Gyanendra Singh</au><au>Chaudhary, Parul</au><au>Pegu, Seema Rani</au><au>Attupurum, Nitin</au><au>Antony, Naveena</au><au>Rajkhowa, Swaraj</au><au>Gupta, Vivek Kumar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and Characterization of Extended-Spectrum β-Lactamase Producing Escherichia coli from Pig Farms and Slaughterhouse</atitle><jtitle>Indian journal of microbiology</jtitle><stitle>Indian J Microbiol</stitle><addtitle>Indian J Microbiol</addtitle><date>2024-09-01</date><risdate>2024</risdate><volume>64</volume><issue>3</issue><spage>950</spage><epage>956</epage><pages>950-956</pages><issn>0046-8991</issn><eissn>0973-7715</eissn><abstract>Extended-spectrum β-lactamase (ESBL) producing
Escherichia coli
represents a formidable challenge in the field of microbiology and public health due to its resistance to commonly used antibiotics. These strains pose a serious threat to human and animal health, underscoring the urgency of comprehensive research and surveillance. The ongoing investigation seeks ESBL producing
E. coli
strains from pig farms and slaughterhouses in West Bengal and Assam, India. A total of 309 samples were collected: nasal swabs (25), rectal swabs (25) from healthy pigs, pig pen soil (45), faeces (55), slaughterhouse effluents (115), and cleaning water (44). In these samples, 154 tested positive for
E. coli
, indicating a 49.8% prevalence. Among 154
E. coli
isolates, 23 (14.9%) produced ESBLs, sourced from pig rectal swabs (7.1%), faeces (10.7%), slaughterhouse effluents (26.1%), and cleaning water (11.7%). Significantly, 4 ESBL
E. coli
isolates (6.6%) exclusively emerged from pig slaughterhouse effluents, displaying imipenem-resistant properties. The majority of ESBL
E. coli
primarily produced CTX-M and CMY, with consistent genetic markers
bla
CTX-M (100%) and
bla
CMY (82.6%). Remarkably, 2 (8.6%) of 17 ESBL
E. coli
isolates from pig slaughterhouse effluents carried the genetic marker
bla
NDM1. These findings stress implementing thorough surveillance in pig farms and local slaughterhouses. This proactive approach is crucial to identify ESBL
E. coli
strains, enhancing public health protection.</abstract><cop>New Delhi</cop><pub>Springer India</pub><pmid>39282198</pmid><doi>10.1007/s12088-023-01151-z</doi><tpages>7</tpages></addata></record> |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Abattoirs Animal health Antibiotics Biomedical and Life Sciences Cleaning E coli Effluents Escherichia coli Farms Feces Genetic markers health promotion Imipenem India Life Sciences Medical Microbiology Microbiology monitoring nose Original Research Article Public health Rectum slaughterhouses soil Soil water Surveillance Swine β Lactamase |
| title | Isolation and Characterization of Extended-Spectrum β-Lactamase Producing Escherichia coli from Pig Farms and Slaughterhouse |
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