Novel Role of the ALPI Gene Associated with Constipation Caused by Complement Component 3 Deficiency

Complement component 3 (C3) deficiency has recently been reported as one of the novel causes of constipation. To identify a unique gene specific to constipation caused by C3 deficiency, the total RNA extracted from the mid colon of C3 knockout (C3 KO) mice was hybridized to oligonucleotide microarra...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:International journal of molecular sciences 2024-09, Vol.25 (17), p.9530
Hauptverfasser: Song, Hee Jin, Kim, Ji Eun, Roh, Yu Jeong, Seol, Ayun, Kim, Tae Ryeol, Park, Ki Ho, Park, Eun Seo, Hong, Jin Tae, Choi, Sun Il, Hwang, Dae Youn
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Complement component 3 (C3) deficiency has recently been reported as one of the novel causes of constipation. To identify a unique gene specific to constipation caused by C3 deficiency, the total RNA extracted from the mid colon of C3 knockout (C3 KO) mice was hybridized to oligonucleotide microarrays, and the function of the candidate gene was verified in in vitro and in vivo models. C3 KO mice used for microarrays showed definite phenotypes of constipation. Overall, compared to the wild type (WT), 1237 genes were upregulated, and 1292 genes were downregulated in the C3 KO mice. Of these, the major genes included were lysine (K)-specific demethylase 5D ( ), olfactory receptor 870 ( ), pancreatic lipase ( ), and alkaline phosphatase intestinal ( ). Specifically, the gene was selected as a novel gene candidate based on alterations during loperamide (Lop)-induced constipation and intestinal bowel disease (IBD). The upregulation of expression treated with acetate recovered the expression level of mucin-related genes in primary epithelial cells of C3 KO mice as well as most phenotypes of constipation in C3 KO mice. These results indicate that plays an important role as the novel gene associated with C3 deficiency-induced constipation.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms25179530