Platinum-perovskite nanocomposite-based Exosensor for specific detection of prostate cancer in clinical settings
Exosomes, extracellular vesicles (EVs) with an average size of 50–150 nm, transfer various biomolecules and exchange signaling molecules between cells in a paracrine manner. Molecular investigations have revealed that EVs can reflect real-time metabolic changes in normal- and cancer-origin cells and...
Gespeichert in:
| Veröffentlicht in: | Mikrochimica acta (1966) 2024-10, Vol.191 (10), p.593, Article 593 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 10 |
| container_start_page | 593 |
| container_title | Mikrochimica acta (1966) |
| container_volume | 191 |
| creator | Dezhakam, Ehsan Mahmoudi, Elham Naseri, Abdolhossein Rahbarghazi, Reza Isildak, Ibrahim Khalilzadeh, Balal Niaei, Aligholi Delibas, Nagihan Coruh, Ali |
| description | Exosomes, extracellular vesicles (EVs) with an average size of 50–150 nm, transfer various biomolecules and exchange signaling molecules between cells in a paracrine manner. Molecular investigations have revealed that EVs can reflect real-time metabolic changes in normal- and cancer-origin cells and thus harbor valid diagnostic biomarkers. Despite these advantages, the detection of low concentrations of cancer cell EVs in biological fluids is still a great challenge. Here, a new electrochemical Exosensor based on platinum-perovskite is developed for the direct detection of EVs using a biotinylated monoclonal CD63 antibody as a capture element. The label-free method exhibited higher sensitivity with a lower limit of quantification of 2000 EVs/µL with a dynamic linear range (LDR) of 2000 to 14,000 EVs/μL compared with other available methods. To enhance the selectivity of detection, EVs were simultaneously sandwiched between secondary antibodies of PSA (prostate-specific antigen), as an FDA-approved prostate cancer biomarker. Data indicated that this Exosensor can distinguish normal and cancer EVs in samples from healthy individuals and prostate cancer patients. Taken together, this technology offers a unique approach to label-free quantification of EVs and cancer detection in the early stages.
Graphical abstract |
| doi_str_mv | 10.1007/s00604-024-06655-z |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3103446310</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3103446310</sourcerecordid><originalsourceid>FETCH-LOGICAL-c256t-b3bf4bcff3e9011b8dc12f06d526b851d377309b575983652f0b0af917206c893</originalsourceid><addsrcrecordid>eNp9kU-LFjEMxoso7uurX8CDFLx4qabttPPOUZb1DyzoQc-l7bRL15l2bGZE99PbdVYFDx5CCPnlScJDyFMOLzlA_woBNHQMRAutlWI398iBd1IzBb28Tw4AQjOpe3FGHiFeA_Bei-4hOZOD0FzK7kCWj5NdU95mtoRavuGXtAaabS6-zEvBVjFnMYz04nvBkLFUGlvgEnyKydMxrMGvqWRaIl1qwdU2AW-zD5WmTP2UcvJ2ohjWtucKH5MH0U4YntzlI_n85uLT-Tt2-eHt-_PXl8wLpVfmpIud8zHKMADn7jR6LiLoUQntToqPsu8lDE71ajhJrVrPgY0D7wVofxrkkbzYddtRX7eAq5kT-jBNNoeyoZEcZNfp23Qkz_9Br8tWc7tup7gaZNcosVO-fYk1RLPUNNv6w3Awt36Y3Q_T_DC__DA3bejZnfTm5jD-GfltQAPkDmBr5atQ_-7-j-xPfZmXfg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3103415934</pqid></control><display><type>article</type><title>Platinum-perovskite nanocomposite-based Exosensor for specific detection of prostate cancer in clinical settings</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Dezhakam, Ehsan ; Mahmoudi, Elham ; Naseri, Abdolhossein ; Rahbarghazi, Reza ; Isildak, Ibrahim ; Khalilzadeh, Balal ; Niaei, Aligholi ; Delibas, Nagihan ; Coruh, Ali</creator><creatorcontrib>Dezhakam, Ehsan ; Mahmoudi, Elham ; Naseri, Abdolhossein ; Rahbarghazi, Reza ; Isildak, Ibrahim ; Khalilzadeh, Balal ; Niaei, Aligholi ; Delibas, Nagihan ; Coruh, Ali</creatorcontrib><description>Exosomes, extracellular vesicles (EVs) with an average size of 50–150 nm, transfer various biomolecules and exchange signaling molecules between cells in a paracrine manner. Molecular investigations have revealed that EVs can reflect real-time metabolic changes in normal- and cancer-origin cells and thus harbor valid diagnostic biomarkers. Despite these advantages, the detection of low concentrations of cancer cell EVs in biological fluids is still a great challenge. Here, a new electrochemical Exosensor based on platinum-perovskite is developed for the direct detection of EVs using a biotinylated monoclonal CD63 antibody as a capture element. The label-free method exhibited higher sensitivity with a lower limit of quantification of 2000 EVs/µL with a dynamic linear range (LDR) of 2000 to 14,000 EVs/μL compared with other available methods. To enhance the selectivity of detection, EVs were simultaneously sandwiched between secondary antibodies of PSA (prostate-specific antigen), as an FDA-approved prostate cancer biomarker. Data indicated that this Exosensor can distinguish normal and cancer EVs in samples from healthy individuals and prostate cancer patients. Taken together, this technology offers a unique approach to label-free quantification of EVs and cancer detection in the early stages.
Graphical abstract</description><identifier>ISSN: 0026-3672</identifier><identifier>ISSN: 1436-5073</identifier><identifier>EISSN: 1436-5073</identifier><identifier>DOI: 10.1007/s00604-024-06655-z</identifier><identifier>PMID: 39261334</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Analytical Chemistry ; Antibodies ; Biomarkers ; Biomarkers, Tumor - analysis ; Biomarkers, Tumor - blood ; Biomarkers, Tumor - immunology ; Biomolecules ; Biosensing Techniques - methods ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Electrochemical Techniques - instrumentation ; Electrochemical Techniques - methods ; Exosomes - chemistry ; Humans ; Labels ; Limit of Detection ; Low concentrations ; Male ; Microengineering ; Nanochemistry ; Nanocomposites ; Nanocomposites - chemistry ; Nanotechnology ; Perovskites ; Platinum ; Platinum - chemistry ; Prostate cancer ; Prostate-Specific Antigen - analysis ; Prostate-Specific Antigen - blood ; Prostate-Specific Antigen - immunology ; Prostatic Neoplasms - diagnosis ; Real time ; Tetraspanin 30 - metabolism</subject><ispartof>Mikrochimica acta (1966), 2024-10, Vol.191 (10), p.593, Article 593</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c256t-b3bf4bcff3e9011b8dc12f06d526b851d377309b575983652f0b0af917206c893</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00604-024-06655-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00604-024-06655-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39261334$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dezhakam, Ehsan</creatorcontrib><creatorcontrib>Mahmoudi, Elham</creatorcontrib><creatorcontrib>Naseri, Abdolhossein</creatorcontrib><creatorcontrib>Rahbarghazi, Reza</creatorcontrib><creatorcontrib>Isildak, Ibrahim</creatorcontrib><creatorcontrib>Khalilzadeh, Balal</creatorcontrib><creatorcontrib>Niaei, Aligholi</creatorcontrib><creatorcontrib>Delibas, Nagihan</creatorcontrib><creatorcontrib>Coruh, Ali</creatorcontrib><title>Platinum-perovskite nanocomposite-based Exosensor for specific detection of prostate cancer in clinical settings</title><title>Mikrochimica acta (1966)</title><addtitle>Microchim Acta</addtitle><addtitle>Mikrochim Acta</addtitle><description>Exosomes, extracellular vesicles (EVs) with an average size of 50–150 nm, transfer various biomolecules and exchange signaling molecules between cells in a paracrine manner. Molecular investigations have revealed that EVs can reflect real-time metabolic changes in normal- and cancer-origin cells and thus harbor valid diagnostic biomarkers. Despite these advantages, the detection of low concentrations of cancer cell EVs in biological fluids is still a great challenge. Here, a new electrochemical Exosensor based on platinum-perovskite is developed for the direct detection of EVs using a biotinylated monoclonal CD63 antibody as a capture element. The label-free method exhibited higher sensitivity with a lower limit of quantification of 2000 EVs/µL with a dynamic linear range (LDR) of 2000 to 14,000 EVs/μL compared with other available methods. To enhance the selectivity of detection, EVs were simultaneously sandwiched between secondary antibodies of PSA (prostate-specific antigen), as an FDA-approved prostate cancer biomarker. Data indicated that this Exosensor can distinguish normal and cancer EVs in samples from healthy individuals and prostate cancer patients. Taken together, this technology offers a unique approach to label-free quantification of EVs and cancer detection in the early stages.
Graphical abstract</description><subject>Analytical Chemistry</subject><subject>Antibodies</subject><subject>Biomarkers</subject><subject>Biomarkers, Tumor - analysis</subject><subject>Biomarkers, Tumor - blood</subject><subject>Biomarkers, Tumor - immunology</subject><subject>Biomolecules</subject><subject>Biosensing Techniques - methods</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Electrochemical Techniques - instrumentation</subject><subject>Electrochemical Techniques - methods</subject><subject>Exosomes - chemistry</subject><subject>Humans</subject><subject>Labels</subject><subject>Limit of Detection</subject><subject>Low concentrations</subject><subject>Male</subject><subject>Microengineering</subject><subject>Nanochemistry</subject><subject>Nanocomposites</subject><subject>Nanocomposites - chemistry</subject><subject>Nanotechnology</subject><subject>Perovskites</subject><subject>Platinum</subject><subject>Platinum - chemistry</subject><subject>Prostate cancer</subject><subject>Prostate-Specific Antigen - analysis</subject><subject>Prostate-Specific Antigen - blood</subject><subject>Prostate-Specific Antigen - immunology</subject><subject>Prostatic Neoplasms - diagnosis</subject><subject>Real time</subject><subject>Tetraspanin 30 - metabolism</subject><issn>0026-3672</issn><issn>1436-5073</issn><issn>1436-5073</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU-LFjEMxoso7uurX8CDFLx4qabttPPOUZb1DyzoQc-l7bRL15l2bGZE99PbdVYFDx5CCPnlScJDyFMOLzlA_woBNHQMRAutlWI398iBd1IzBb28Tw4AQjOpe3FGHiFeA_Bei-4hOZOD0FzK7kCWj5NdU95mtoRavuGXtAaabS6-zEvBVjFnMYz04nvBkLFUGlvgEnyKydMxrMGvqWRaIl1qwdU2AW-zD5WmTP2UcvJ2ohjWtucKH5MH0U4YntzlI_n85uLT-Tt2-eHt-_PXl8wLpVfmpIud8zHKMADn7jR6LiLoUQntToqPsu8lDE71ajhJrVrPgY0D7wVofxrkkbzYddtRX7eAq5kT-jBNNoeyoZEcZNfp23Qkz_9Br8tWc7tup7gaZNcosVO-fYk1RLPUNNv6w3Awt36Y3Q_T_DC__DA3bejZnfTm5jD-GfltQAPkDmBr5atQ_-7-j-xPfZmXfg</recordid><startdate>20241001</startdate><enddate>20241001</enddate><creator>Dezhakam, Ehsan</creator><creator>Mahmoudi, Elham</creator><creator>Naseri, Abdolhossein</creator><creator>Rahbarghazi, Reza</creator><creator>Isildak, Ibrahim</creator><creator>Khalilzadeh, Balal</creator><creator>Niaei, Aligholi</creator><creator>Delibas, Nagihan</creator><creator>Coruh, Ali</creator><general>Springer Vienna</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>20241001</creationdate><title>Platinum-perovskite nanocomposite-based Exosensor for specific detection of prostate cancer in clinical settings</title><author>Dezhakam, Ehsan ; Mahmoudi, Elham ; Naseri, Abdolhossein ; Rahbarghazi, Reza ; Isildak, Ibrahim ; Khalilzadeh, Balal ; Niaei, Aligholi ; Delibas, Nagihan ; Coruh, Ali</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c256t-b3bf4bcff3e9011b8dc12f06d526b851d377309b575983652f0b0af917206c893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Analytical Chemistry</topic><topic>Antibodies</topic><topic>Biomarkers</topic><topic>Biomarkers, Tumor - analysis</topic><topic>Biomarkers, Tumor - blood</topic><topic>Biomarkers, Tumor - immunology</topic><topic>Biomolecules</topic><topic>Biosensing Techniques - methods</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Electrochemical Techniques - instrumentation</topic><topic>Electrochemical Techniques - methods</topic><topic>Exosomes - chemistry</topic><topic>Humans</topic><topic>Labels</topic><topic>Limit of Detection</topic><topic>Low concentrations</topic><topic>Male</topic><topic>Microengineering</topic><topic>Nanochemistry</topic><topic>Nanocomposites</topic><topic>Nanocomposites - chemistry</topic><topic>Nanotechnology</topic><topic>Perovskites</topic><topic>Platinum</topic><topic>Platinum - chemistry</topic><topic>Prostate cancer</topic><topic>Prostate-Specific Antigen - analysis</topic><topic>Prostate-Specific Antigen - blood</topic><topic>Prostate-Specific Antigen - immunology</topic><topic>Prostatic Neoplasms - diagnosis</topic><topic>Real time</topic><topic>Tetraspanin 30 - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dezhakam, Ehsan</creatorcontrib><creatorcontrib>Mahmoudi, Elham</creatorcontrib><creatorcontrib>Naseri, Abdolhossein</creatorcontrib><creatorcontrib>Rahbarghazi, Reza</creatorcontrib><creatorcontrib>Isildak, Ibrahim</creatorcontrib><creatorcontrib>Khalilzadeh, Balal</creatorcontrib><creatorcontrib>Niaei, Aligholi</creatorcontrib><creatorcontrib>Delibas, Nagihan</creatorcontrib><creatorcontrib>Coruh, Ali</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Mikrochimica acta (1966)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dezhakam, Ehsan</au><au>Mahmoudi, Elham</au><au>Naseri, Abdolhossein</au><au>Rahbarghazi, Reza</au><au>Isildak, Ibrahim</au><au>Khalilzadeh, Balal</au><au>Niaei, Aligholi</au><au>Delibas, Nagihan</au><au>Coruh, Ali</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Platinum-perovskite nanocomposite-based Exosensor for specific detection of prostate cancer in clinical settings</atitle><jtitle>Mikrochimica acta (1966)</jtitle><stitle>Microchim Acta</stitle><addtitle>Mikrochim Acta</addtitle><date>2024-10-01</date><risdate>2024</risdate><volume>191</volume><issue>10</issue><spage>593</spage><pages>593-</pages><artnum>593</artnum><issn>0026-3672</issn><issn>1436-5073</issn><eissn>1436-5073</eissn><abstract>Exosomes, extracellular vesicles (EVs) with an average size of 50–150 nm, transfer various biomolecules and exchange signaling molecules between cells in a paracrine manner. Molecular investigations have revealed that EVs can reflect real-time metabolic changes in normal- and cancer-origin cells and thus harbor valid diagnostic biomarkers. Despite these advantages, the detection of low concentrations of cancer cell EVs in biological fluids is still a great challenge. Here, a new electrochemical Exosensor based on platinum-perovskite is developed for the direct detection of EVs using a biotinylated monoclonal CD63 antibody as a capture element. The label-free method exhibited higher sensitivity with a lower limit of quantification of 2000 EVs/µL with a dynamic linear range (LDR) of 2000 to 14,000 EVs/μL compared with other available methods. To enhance the selectivity of detection, EVs were simultaneously sandwiched between secondary antibodies of PSA (prostate-specific antigen), as an FDA-approved prostate cancer biomarker. Data indicated that this Exosensor can distinguish normal and cancer EVs in samples from healthy individuals and prostate cancer patients. Taken together, this technology offers a unique approach to label-free quantification of EVs and cancer detection in the early stages.
Graphical abstract</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><pmid>39261334</pmid><doi>10.1007/s00604-024-06655-z</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0026-3672 |
| ispartof | Mikrochimica acta (1966), 2024-10, Vol.191 (10), p.593, Article 593 |
| issn | 0026-3672 1436-5073 1436-5073 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_3103446310 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Analytical Chemistry Antibodies Biomarkers Biomarkers, Tumor - analysis Biomarkers, Tumor - blood Biomarkers, Tumor - immunology Biomolecules Biosensing Techniques - methods Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Electrochemical Techniques - instrumentation Electrochemical Techniques - methods Exosomes - chemistry Humans Labels Limit of Detection Low concentrations Male Microengineering Nanochemistry Nanocomposites Nanocomposites - chemistry Nanotechnology Perovskites Platinum Platinum - chemistry Prostate cancer Prostate-Specific Antigen - analysis Prostate-Specific Antigen - blood Prostate-Specific Antigen - immunology Prostatic Neoplasms - diagnosis Real time Tetraspanin 30 - metabolism |
| title | Platinum-perovskite nanocomposite-based Exosensor for specific detection of prostate cancer in clinical settings |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T22%3A53%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Platinum-perovskite%20nanocomposite-based%20Exosensor%20for%20specific%20detection%20of%20prostate%20cancer%20in%20clinical%20settings&rft.jtitle=Mikrochimica%20acta%20(1966)&rft.au=Dezhakam,%20Ehsan&rft.date=2024-10-01&rft.volume=191&rft.issue=10&rft.spage=593&rft.pages=593-&rft.artnum=593&rft.issn=0026-3672&rft.eissn=1436-5073&rft_id=info:doi/10.1007/s00604-024-06655-z&rft_dat=%3Cproquest_cross%3E3103446310%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3103415934&rft_id=info:pmid/39261334&rfr_iscdi=true |