Optimizing operation strategy to improve storage of intracellular carbon sources in anaerobic/oxic/anoxic system: Enhanced nitrogen removal by endogenous denitrification

Endogenous denitrification (ED) can make full use of the carbon sources and avoid replenishment of it. However, strengthening the storage of intracellular carbon sources is an important factor in improving ED efficiency. In this study, employed batch experiments using real domestic wastewater in the anaerobic/oxic (A/O) process. The anaerobic and oxic processes were run for 4 h under ambient conditions with the dissolved oxygen (DO) concentrations in the oxic stage controlled at 0.5, 1.0, 1.5, and 3.0 mg/L, respectively. The results showed that the content of poly-β-hydroxyalkanoates (PHA) reached its peak at 60 min (1.25 mmolC/L). And with DO concentrations of 1.5 mg/L, the contents of glycogen (Gly) were 27.74 mmolC/L. Subsequently, the AOA-SBR was established to investigate its effect on the long-term nitrogen removal performance of domestic wastewater by optimizing the anaerobic time and DO concentrations. The results showed that at an anaerobic time of 60 min and DO concentration of 1.5 mg/L, the storage of the intracellular carbon sources was highest and the total nitrogen (TN) removal efficiency increased to 82.12%. In addition, Candidatus Competibacter dominated gradually in the system as the strategy was optimized. [Display omitted] •Optimization of anaerobic time improves intracellular carbon sources storage.•Glycogen synthesis reached 3.87 mmolC/L when DO was 1.5 mg/L in the oxic stage.•Candidatus Competibacter could conduct ED and its relative abundance reach to 6.59%.•The total nitrogen removal efficiency was enhanced by 11.59% in the AOA system.