Functional Analysis of the Channelrhodopsin Genes from the Green Algae of the White Sea Basin
Optogenetics, the method of light-controlled regulation of cellular processes is based on the use of the channelrhodopsins that directly generate photoinduced currents. Most of the channelrhodopsin genes have been identified in the green microalgae Chlorophyta, and the demand for increasing the numb...
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| Veröffentlicht in: | Biochemistry (Moscow) 2024-08, Vol.89 (8), p.1392-1401 |
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| creator | Karpova, Olga V. Vinogradova, Elizaveta N. Moisenovich, Anastasiya M. Pustovit, Oksana B. Ramonova, Alla A. Abramochkin, Denis V. Lobakova, Elena S. |
| description | Optogenetics, the method of light-controlled regulation of cellular processes is based on the use of the channelrhodopsins that directly generate photoinduced currents. Most of the channelrhodopsin genes have been identified in the green microalgae Chlorophyta, and the demand for increasing the number of functionally characterized channelrhodopsins and the diversity of their photochemical parameters keeps growing. We performed the expression analysis of cation channelrhodopsin (CCR) genes in natural isolates of microalgae of the genera
Haematococcus
and
Bracteacoccus
from the unique Arctic Circle region. The identified full-length CCR transcript of
H. lacustris
is the product of alternative splicing and encodes the Hl98CCR2 protein with no photochemical activity. The 5′-partial fragment of the
B. aggregatus
CCR transcript encodes the Ba34CCR protein containing a conserved TM1-TM7 membrane domain and a short cytosolic fragment. Upon heterologous expression of the TM1-TM7 fragment in CHO-K1 cell culture, light-dependent current generation was observed with the parameters corresponding to those of the CCR. The first discovered functional channelrhodopsin of
Bracteacoccus
has no close CCR homologues and may be of interest as a candidate for optogenetics. |
| doi_str_mv | 10.1134/S0006297924080030 |
| format | Article |
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Haematococcus
and
Bracteacoccus
from the unique Arctic Circle region. The identified full-length CCR transcript of
H. lacustris
is the product of alternative splicing and encodes the Hl98CCR2 protein with no photochemical activity. The 5′-partial fragment of the
B. aggregatus
CCR transcript encodes the Ba34CCR protein containing a conserved TM1-TM7 membrane domain and a short cytosolic fragment. Upon heterologous expression of the TM1-TM7 fragment in CHO-K1 cell culture, light-dependent current generation was observed with the parameters corresponding to those of the CCR. The first discovered functional channelrhodopsin of
Bracteacoccus
has no close CCR homologues and may be of interest as a candidate for optogenetics.</description><identifier>ISSN: 0006-2979</identifier><identifier>ISSN: 1608-3040</identifier><identifier>EISSN: 1608-3040</identifier><identifier>DOI: 10.1134/S0006297924080030</identifier><identifier>PMID: 39245452</identifier><language>eng</language><publisher>Moscow: Pleiades Publishing</publisher><subject>Algae ; Alternative splicing ; Animals ; Aquatic microorganisms ; Aquatic plants ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Bioorganic Chemistry ; Bracteacoccus ; Cell culture ; Channelrhodopsins - genetics ; Channelrhodopsins - metabolism ; Chlorophyta - genetics ; Chlorophyta - metabolism ; CHO Cells ; Cricetulus ; Demand analysis ; Functional analysis ; Gene regulation ; Genes ; Genetics ; Information processing ; Life Sciences ; Light ; Microalgae ; Microbiology ; Optics ; Optogenetics - methods ; Parameter identification ; Photochemicals ; Photochemistry ; Protein expression ; Proteins ; Sea currents</subject><ispartof>Biochemistry (Moscow), 2024-08, Vol.89 (8), p.1392-1401</ispartof><rights>The Author(s) 2024</rights><rights>The Author(s) 2024. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c297t-74346e84c69d97cfbae3cfe644b8e10525310e6058ee3b964614a2c60221033b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S0006297924080030$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S0006297924080030$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39245452$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Karpova, Olga V.</creatorcontrib><creatorcontrib>Vinogradova, Elizaveta N.</creatorcontrib><creatorcontrib>Moisenovich, Anastasiya M.</creatorcontrib><creatorcontrib>Pustovit, Oksana B.</creatorcontrib><creatorcontrib>Ramonova, Alla A.</creatorcontrib><creatorcontrib>Abramochkin, Denis V.</creatorcontrib><creatorcontrib>Lobakova, Elena S.</creatorcontrib><title>Functional Analysis of the Channelrhodopsin Genes from the Green Algae of the White Sea Basin</title><title>Biochemistry (Moscow)</title><addtitle>Biochemistry Moscow</addtitle><addtitle>Biochemistry (Mosc)</addtitle><description>Optogenetics, the method of light-controlled regulation of cellular processes is based on the use of the channelrhodopsins that directly generate photoinduced currents. Most of the channelrhodopsin genes have been identified in the green microalgae Chlorophyta, and the demand for increasing the number of functionally characterized channelrhodopsins and the diversity of their photochemical parameters keeps growing. We performed the expression analysis of cation channelrhodopsin (CCR) genes in natural isolates of microalgae of the genera
Haematococcus
and
Bracteacoccus
from the unique Arctic Circle region. The identified full-length CCR transcript of
H. lacustris
is the product of alternative splicing and encodes the Hl98CCR2 protein with no photochemical activity. The 5′-partial fragment of the
B. aggregatus
CCR transcript encodes the Ba34CCR protein containing a conserved TM1-TM7 membrane domain and a short cytosolic fragment. Upon heterologous expression of the TM1-TM7 fragment in CHO-K1 cell culture, light-dependent current generation was observed with the parameters corresponding to those of the CCR. The first discovered functional channelrhodopsin of
Bracteacoccus
has no close CCR homologues and may be of interest as a candidate for optogenetics.</description><subject>Algae</subject><subject>Alternative splicing</subject><subject>Animals</subject><subject>Aquatic microorganisms</subject><subject>Aquatic plants</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Bioorganic Chemistry</subject><subject>Bracteacoccus</subject><subject>Cell culture</subject><subject>Channelrhodopsins - genetics</subject><subject>Channelrhodopsins - metabolism</subject><subject>Chlorophyta - genetics</subject><subject>Chlorophyta - metabolism</subject><subject>CHO Cells</subject><subject>Cricetulus</subject><subject>Demand analysis</subject><subject>Functional analysis</subject><subject>Gene regulation</subject><subject>Genes</subject><subject>Genetics</subject><subject>Information processing</subject><subject>Life Sciences</subject><subject>Light</subject><subject>Microalgae</subject><subject>Microbiology</subject><subject>Optics</subject><subject>Optogenetics - methods</subject><subject>Parameter identification</subject><subject>Photochemicals</subject><subject>Photochemistry</subject><subject>Protein expression</subject><subject>Proteins</subject><subject>Sea currents</subject><issn>0006-2979</issn><issn>1608-3040</issn><issn>1608-3040</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><recordid>eNp1kE9PwjAYhxujEUQ_gBfTxIuX6ds_K9sRiaAJiQc0nszSjXcwMlpstwPf3iKgicZLm-b3PG_bHyGXDG4ZE_JuCgCKp_2US0gABByRLlOQRAIkHJPuNo62eYeceb8MRw6pOCUdEYxYxrxL3ketKZrKGl3TQVg2vvLUlrRZIB0utDFYu4Wd2bWvDB2jQU9LZ1df-dghGjqo5xoPytuiapBOUdN7HYxzclLq2uPFfu-R19HDy_AxmjyPn4aDSVSE1zVRXwqpMJGFSmdpvyhzjaIoUUmZJ8gg5rFggAriBFHkqZKKSc0LBZwzECIXPXKzm7t29qNF32SryhdY19qgbX0WdA59iCEO6PUvdGlbF34eKEiTVHAlRKDYjiqc9d5hma1dtdJukzHItt1nf7oPztV-cpuvcPZtHMoOAN8BPkRmju7n6v-nfgI9w4r3</recordid><startdate>20240801</startdate><enddate>20240801</enddate><creator>Karpova, Olga V.</creator><creator>Vinogradova, Elizaveta N.</creator><creator>Moisenovich, Anastasiya M.</creator><creator>Pustovit, Oksana B.</creator><creator>Ramonova, Alla A.</creator><creator>Abramochkin, Denis V.</creator><creator>Lobakova, Elena S.</creator><general>Pleiades Publishing</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>7U9</scope><scope>C1K</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20240801</creationdate><title>Functional Analysis of the Channelrhodopsin Genes from the Green Algae of the White Sea Basin</title><author>Karpova, Olga V. ; Vinogradova, Elizaveta N. ; Moisenovich, Anastasiya M. ; Pustovit, Oksana B. ; Ramonova, Alla A. ; Abramochkin, Denis V. ; Lobakova, Elena S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c297t-74346e84c69d97cfbae3cfe644b8e10525310e6058ee3b964614a2c60221033b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Algae</topic><topic>Alternative splicing</topic><topic>Animals</topic><topic>Aquatic microorganisms</topic><topic>Aquatic plants</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Bioorganic Chemistry</topic><topic>Bracteacoccus</topic><topic>Cell culture</topic><topic>Channelrhodopsins - genetics</topic><topic>Channelrhodopsins - metabolism</topic><topic>Chlorophyta - genetics</topic><topic>Chlorophyta - metabolism</topic><topic>CHO Cells</topic><topic>Cricetulus</topic><topic>Demand analysis</topic><topic>Functional analysis</topic><topic>Gene regulation</topic><topic>Genes</topic><topic>Genetics</topic><topic>Information processing</topic><topic>Life Sciences</topic><topic>Light</topic><topic>Microalgae</topic><topic>Microbiology</topic><topic>Optics</topic><topic>Optogenetics - methods</topic><topic>Parameter identification</topic><topic>Photochemicals</topic><topic>Photochemistry</topic><topic>Protein expression</topic><topic>Proteins</topic><topic>Sea currents</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Karpova, Olga V.</creatorcontrib><creatorcontrib>Vinogradova, Elizaveta N.</creatorcontrib><creatorcontrib>Moisenovich, Anastasiya M.</creatorcontrib><creatorcontrib>Pustovit, Oksana B.</creatorcontrib><creatorcontrib>Ramonova, Alla A.</creatorcontrib><creatorcontrib>Abramochkin, Denis V.</creatorcontrib><creatorcontrib>Lobakova, Elena S.</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemistry (Moscow)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Karpova, Olga V.</au><au>Vinogradova, Elizaveta N.</au><au>Moisenovich, Anastasiya M.</au><au>Pustovit, Oksana B.</au><au>Ramonova, Alla A.</au><au>Abramochkin, Denis V.</au><au>Lobakova, Elena S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Functional Analysis of the Channelrhodopsin Genes from the Green Algae of the White Sea Basin</atitle><jtitle>Biochemistry (Moscow)</jtitle><stitle>Biochemistry Moscow</stitle><addtitle>Biochemistry (Mosc)</addtitle><date>2024-08-01</date><risdate>2024</risdate><volume>89</volume><issue>8</issue><spage>1392</spage><epage>1401</epage><pages>1392-1401</pages><issn>0006-2979</issn><issn>1608-3040</issn><eissn>1608-3040</eissn><abstract>Optogenetics, the method of light-controlled regulation of cellular processes is based on the use of the channelrhodopsins that directly generate photoinduced currents. 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Haematococcus
and
Bracteacoccus
from the unique Arctic Circle region. The identified full-length CCR transcript of
H. lacustris
is the product of alternative splicing and encodes the Hl98CCR2 protein with no photochemical activity. The 5′-partial fragment of the
B. aggregatus
CCR transcript encodes the Ba34CCR protein containing a conserved TM1-TM7 membrane domain and a short cytosolic fragment. Upon heterologous expression of the TM1-TM7 fragment in CHO-K1 cell culture, light-dependent current generation was observed with the parameters corresponding to those of the CCR. The first discovered functional channelrhodopsin of
Bracteacoccus
has no close CCR homologues and may be of interest as a candidate for optogenetics.</abstract><cop>Moscow</cop><pub>Pleiades Publishing</pub><pmid>39245452</pmid><doi>10.1134/S0006297924080030</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Biochemistry (Moscow), 2024-08, Vol.89 (8), p.1392-1401 |
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| subjects | Algae Alternative splicing Animals Aquatic microorganisms Aquatic plants Biochemistry Biomedical and Life Sciences Biomedicine Bioorganic Chemistry Bracteacoccus Cell culture Channelrhodopsins - genetics Channelrhodopsins - metabolism Chlorophyta - genetics Chlorophyta - metabolism CHO Cells Cricetulus Demand analysis Functional analysis Gene regulation Genes Genetics Information processing Life Sciences Light Microalgae Microbiology Optics Optogenetics - methods Parameter identification Photochemicals Photochemistry Protein expression Proteins Sea currents |
| title | Functional Analysis of the Channelrhodopsin Genes from the Green Algae of the White Sea Basin |
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