LncRNA SNHG1 facilitates colorectal cancer cells metastasis by recruiting HNRNPD protein to stabilize SERPINA3 mRNA

Metastasis continues to negatively impact individuals diagnosed with colorectal cancer (CRC). Research has revealed the important role of long noncoding RNAs (lncRNAs) in CRC metastasis, but the underlying mechanisms remain unclear. Here, we revealed that the lncRNA small nucleolar RNA host gene 1 (SNHG1) is expressed at higher levels in metastatic CRC tissues than in primary CRC tissues, and that high lncRNA SNHG1 expression indicates poor patient outcomes. We found that lncRNA SNHG1 promotes the migration and invasion of tumor cells both in vivo and in vitro. Moreover, lncRNA SNHG1 increases serpin family A member 3 (SERPINA3) mRNA stability by interacting with the heterogeneous nuclear ribonucleoprotein D (HNRNPD) protein, and subsequently upregulates SERPINA3 expression. Moreover, HNRNPD and SERPINA3 reversed the effects of lncRNA SNHG1 knockdown on CRC cell metastasis. In conclusion, we report that the lncRNA SNHG1 recruits HNRNPD, in turn upregulating SERPINA3 expression and ultimately facilitating CRC cell migration and invasion. Targeting the lncRNA SNHG1/HNRNPD/SERPINA3 signaling pathway might be a therapeutic option for preventing CRC metastasis. •Overexpression of lncRNA SNHG1 predicts a poor prognosis in CRC patients and promotes CRC cells metastasis.•LncRNA SNHG1 promotes CRC metastasis via SERPINA3 and upregulates SERPINA3 expression by enhancing its mRNA stability.•LncRNA SNHG1 interacts with HNRNPD protein without affecting HNRNPD expression.•HNRNPD binds to the 3′UTR of SERPINA3 mRNA and enhances SERPINA3 mRNA stability.•LncRNA SNHG1 binds to the 5′UTR of SERPINA3 mRNA and stabilizes SERPINA3 mRNA in a manner dependent on HNRNPD.