A Cost‐Effective Approach for Single‐Stranded DNA Amplification Using Primer‐Blocked Asymmetric PCR

In vitro amplification of single‐stranded oligonucleotide libraries presents a significant challenge due to the potential for excessive byproduct formation. This phenomenon largely affects the quality of the ssDNAs created using the most commonly used methods, e.g., asymmetric PCR, biotin‐streptavid...

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Veröffentlicht in:	Current protocols 2024-09, Vol.4 (9), p.e1125-n/a
Hauptverfasser:	Percze, Krisztina, Harkai, Ákos, Mészáros, Tamás
Format:	Artikel
Sprache:	eng
Schlagworte:	asymmetric PCR Cost-Benefit Analysis DNA Primers - genetics DNA, Single-Stranded - chemistry DNA, Single-Stranded - genetics DNA, Single-Stranded - isolation & purification Polymerase Chain Reaction - economics Polymerase Chain Reaction - methods ssDNA production ssDNA purification
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