Relationship between microorganisms and milk metabolites during quality changes in refrigerated raw milk: A metagenomic and metabolomic exploration
Although cold storage at 4 °C can effectively prolong the shelf life of raw milk, it cannot prevent its eventual spoilage. In this study, we analyzed the main physicochemical and microbial indexes of raw milk stored at 4 °C for 6 days. The changes in microbial profiles and milk metabolites and their...
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| Veröffentlicht in: | International journal of food microbiology 2024-12, Vol.425, p.110891, Article 110891 |
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| container_title | International journal of food microbiology |
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| creator | Zhuang, Jiao Hou, Yanru Wang, Yuanyuan Gao, Yan Chen, Yanhui Qi, Jin Li, Puyu Bian, Yongxia Ju, Ning |
| description | Although cold storage at 4 °C can effectively prolong the shelf life of raw milk, it cannot prevent its eventual spoilage. In this study, we analyzed the main physicochemical and microbial indexes of raw milk stored at 4 °C for 6 days. The changes in microbial profiles and milk metabolites and their relationship during refrigeration were also explored. Metagenomic analysis performed using the Illumina Hiseq Xten sequencing platform revealed that the dominant genera in raw milk evolved from Acinetobacter, Streptococcus, Staphylococcus, and Anaplasma to Flavobacterium, Pseudomonas, and Lactococcus during cold storage. Using the UHPLC-Q-TOF MS method, 77 significantly different metabolites (p |
| doi_str_mv | 10.1016/j.ijfoodmicro.2024.110891 |
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•The dynamic relationship between microbial succession and metabolites was explored.•Complex microbial succession occurred during refrigeration.•Thirty-seven lipids were significantly altered across different stages of raw milk refrigeration.•Psychrotrophic microorganisms showed a significant positive correlation with milk metabolites.•The key stage for quality control in raw milk was found to be 3–4 days.</description><identifier>ISSN: 0168-1605</identifier><identifier>ISSN: 1879-3460</identifier><identifier>EISSN: 1879-3460</identifier><identifier>DOI: 10.1016/j.ijfoodmicro.2024.110891</identifier><identifier>PMID: 39216362</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Bacteria - classification ; Bacteria - genetics ; Bacteria - isolation & purification ; Bacteria - metabolism ; Cattle ; Cold storage ; Food Microbiology ; Food Storage ; Metabolomics ; Metagenomics ; Milk - microbiology ; Quality changes ; Raw milk ; Refrigeration</subject><ispartof>International journal of food microbiology, 2024-12, Vol.425, p.110891, Article 110891</ispartof><rights>2024 Elsevier B.V.</rights><rights>Copyright © 2024 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c251t-9f3ad5a7516aace05738cea95198674b56f76558d7c15eb9140f218c591b37833</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijfoodmicro.2024.110891$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39216362$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhuang, Jiao</creatorcontrib><creatorcontrib>Hou, Yanru</creatorcontrib><creatorcontrib>Wang, Yuanyuan</creatorcontrib><creatorcontrib>Gao, Yan</creatorcontrib><creatorcontrib>Chen, Yanhui</creatorcontrib><creatorcontrib>Qi, Jin</creatorcontrib><creatorcontrib>Li, Puyu</creatorcontrib><creatorcontrib>Bian, Yongxia</creatorcontrib><creatorcontrib>Ju, Ning</creatorcontrib><title>Relationship between microorganisms and milk metabolites during quality changes in refrigerated raw milk: A metagenomic and metabolomic exploration</title><title>International journal of food microbiology</title><addtitle>Int J Food Microbiol</addtitle><description>Although cold storage at 4 °C can effectively prolong the shelf life of raw milk, it cannot prevent its eventual spoilage. In this study, we analyzed the main physicochemical and microbial indexes of raw milk stored at 4 °C for 6 days. The changes in microbial profiles and milk metabolites and their relationship during refrigeration were also explored. Metagenomic analysis performed using the Illumina Hiseq Xten sequencing platform revealed that the dominant genera in raw milk evolved from Acinetobacter, Streptococcus, Staphylococcus, and Anaplasma to Flavobacterium, Pseudomonas, and Lactococcus during cold storage. Using the UHPLC-Q-TOF MS method, 77 significantly different metabolites (p < 0.05) were identified, among which lipids were the most abundant (37). The most significant metabolic changes largely occurred at 3–4 days of refrigeration, coinciding with the rapid increase in dominant psychrotrophic bacteria. Subsequently, correlation analysis demonstrated that these lipid-related metabolites were significantly associated with Acinetobacter, Flavobacterium, and Pseudomonas. Both macro indicators and microanalysis indicated that the key stage of quality changes in raw milk was 3–4 days. Thus, this stage can be targeted for the quality control of raw milk.
•The dynamic relationship between microbial succession and metabolites was explored.•Complex microbial succession occurred during refrigeration.•Thirty-seven lipids were significantly altered across different stages of raw milk refrigeration.•Psychrotrophic microorganisms showed a significant positive correlation with milk metabolites.•The key stage for quality control in raw milk was found to be 3–4 days.</description><subject>Animals</subject><subject>Bacteria - classification</subject><subject>Bacteria - genetics</subject><subject>Bacteria - isolation & purification</subject><subject>Bacteria - metabolism</subject><subject>Cattle</subject><subject>Cold storage</subject><subject>Food Microbiology</subject><subject>Food Storage</subject><subject>Metabolomics</subject><subject>Metagenomics</subject><subject>Milk - microbiology</subject><subject>Quality changes</subject><subject>Raw milk</subject><subject>Refrigeration</subject><issn>0168-1605</issn><issn>1879-3460</issn><issn>1879-3460</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkcFu1DAQhi1ERZfCKyBz45LFjteOza1aQUGqhFS1Z8uxJ6mXxN7aCaXP0RfGTQriyMma0f__M-MPofeUbCmh4uNh6w9djG70NsVtTerdllIiFX2BNlQ2qmI7QV6iTdHKigrCT9HrnA-EEM4YeYVOmaqpYKLeoMcrGMzkY8i3_ohbmO4BAl6CY-pN8HnM2ARXWsMPPMJk2jj4CTJ2c_Khx3ezKfUDtrcm9KXtA07QJd9DMhM4nMz94v2Ezxd7DyGW-DVzjVtq-HUcYlpWeYNOOjNkePv8nqGbL5-v91-ry-8X3_bnl5WtOZ0q1THjuGk4FcZYILxh0oJRnCopml3LRdcIzqVrLOXQKrojXU2l5Yq2rJGMnaEPa-4xxbsZ8qRHny0MgwkQ56wZUUqSRjBZpGqVln_JuRyoj8mPJj1oSvQTE33Q_zDRT0z0yqR43z2PmdsR3F_nHwhFsF8FUI796SHpbD0EC84nsJN20f_HmN9cOabp</recordid><startdate>20241202</startdate><enddate>20241202</enddate><creator>Zhuang, Jiao</creator><creator>Hou, Yanru</creator><creator>Wang, Yuanyuan</creator><creator>Gao, Yan</creator><creator>Chen, Yanhui</creator><creator>Qi, Jin</creator><creator>Li, Puyu</creator><creator>Bian, Yongxia</creator><creator>Ju, Ning</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20241202</creationdate><title>Relationship between microorganisms and milk metabolites during quality changes in refrigerated raw milk: A metagenomic and metabolomic exploration</title><author>Zhuang, Jiao ; Hou, Yanru ; Wang, Yuanyuan ; Gao, Yan ; Chen, Yanhui ; Qi, Jin ; Li, Puyu ; Bian, Yongxia ; Ju, Ning</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c251t-9f3ad5a7516aace05738cea95198674b56f76558d7c15eb9140f218c591b37833</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Animals</topic><topic>Bacteria - classification</topic><topic>Bacteria - genetics</topic><topic>Bacteria - isolation & purification</topic><topic>Bacteria - metabolism</topic><topic>Cattle</topic><topic>Cold storage</topic><topic>Food Microbiology</topic><topic>Food Storage</topic><topic>Metabolomics</topic><topic>Metagenomics</topic><topic>Milk - microbiology</topic><topic>Quality changes</topic><topic>Raw milk</topic><topic>Refrigeration</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhuang, Jiao</creatorcontrib><creatorcontrib>Hou, Yanru</creatorcontrib><creatorcontrib>Wang, Yuanyuan</creatorcontrib><creatorcontrib>Gao, Yan</creatorcontrib><creatorcontrib>Chen, Yanhui</creatorcontrib><creatorcontrib>Qi, Jin</creatorcontrib><creatorcontrib>Li, Puyu</creatorcontrib><creatorcontrib>Bian, Yongxia</creatorcontrib><creatorcontrib>Ju, Ning</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of food microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhuang, Jiao</au><au>Hou, Yanru</au><au>Wang, Yuanyuan</au><au>Gao, Yan</au><au>Chen, Yanhui</au><au>Qi, Jin</au><au>Li, Puyu</au><au>Bian, Yongxia</au><au>Ju, Ning</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Relationship between microorganisms and milk metabolites during quality changes in refrigerated raw milk: A metagenomic and metabolomic exploration</atitle><jtitle>International journal of food microbiology</jtitle><addtitle>Int J Food Microbiol</addtitle><date>2024-12-02</date><risdate>2024</risdate><volume>425</volume><spage>110891</spage><pages>110891-</pages><artnum>110891</artnum><issn>0168-1605</issn><issn>1879-3460</issn><eissn>1879-3460</eissn><abstract>Although cold storage at 4 °C can effectively prolong the shelf life of raw milk, it cannot prevent its eventual spoilage. In this study, we analyzed the main physicochemical and microbial indexes of raw milk stored at 4 °C for 6 days. The changes in microbial profiles and milk metabolites and their relationship during refrigeration were also explored. Metagenomic analysis performed using the Illumina Hiseq Xten sequencing platform revealed that the dominant genera in raw milk evolved from Acinetobacter, Streptococcus, Staphylococcus, and Anaplasma to Flavobacterium, Pseudomonas, and Lactococcus during cold storage. Using the UHPLC-Q-TOF MS method, 77 significantly different metabolites (p < 0.05) were identified, among which lipids were the most abundant (37). The most significant metabolic changes largely occurred at 3–4 days of refrigeration, coinciding with the rapid increase in dominant psychrotrophic bacteria. Subsequently, correlation analysis demonstrated that these lipid-related metabolites were significantly associated with Acinetobacter, Flavobacterium, and Pseudomonas. Both macro indicators and microanalysis indicated that the key stage of quality changes in raw milk was 3–4 days. Thus, this stage can be targeted for the quality control of raw milk.
•The dynamic relationship between microbial succession and metabolites was explored.•Complex microbial succession occurred during refrigeration.•Thirty-seven lipids were significantly altered across different stages of raw milk refrigeration.•Psychrotrophic microorganisms showed a significant positive correlation with milk metabolites.•The key stage for quality control in raw milk was found to be 3–4 days.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>39216362</pmid><doi>10.1016/j.ijfoodmicro.2024.110891</doi></addata></record> |
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| subjects | Animals Bacteria - classification Bacteria - genetics Bacteria - isolation & purification Bacteria - metabolism Cattle Cold storage Food Microbiology Food Storage Metabolomics Metagenomics Milk - microbiology Quality changes Raw milk Refrigeration |
| title | Relationship between microorganisms and milk metabolites during quality changes in refrigerated raw milk: A metagenomic and metabolomic exploration |
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