The Sec pathway gene yidC regulates the virulence of mesophilic Aeromonassalmonicida
Aeromonas salmonicida is a common pathogenic bacterial species found in both freshwater and marine fish, leading to significant economic losses in the aquaculture industry. YidC is an accessory to SecYEG and is essential for the SecYEG transporter to insert into the bacterial membrane. However, the...
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description | Aeromonas salmonicida is a common pathogenic bacterial species found in both freshwater and marine fish, leading to significant economic losses in the aquaculture industry. YidC is an accessory to SecYEG and is essential for the SecYEG transporter to insert into the bacterial membrane. However, the roles of the yidC gene on the host immune response remain unclear. Here, we compared the pathogenicity of yidC gene-deleted (ΔyidC) strain and wild-type (SRW-OG1) strain of mesophilic A. salmonicida to Orange-spotted grouper (Epinephelus coioides), and explored the impacts of yidC gene on the immune response of E. coioides to mesophilic A. salmonicida infection by using Red/ET recombineering. In this study, the E. coioides in the Secondary infected group had a 53.9 % higher survival rate than those in the Primary infected group. In addition, the adhesion ability of ΔyidC strain decreased by about 83.36 % compared with that of the wild-type (SRW-OG1) strain. Further comparison of the biological phenotype of SRW-OG1 and ΔyidC revealed that this yidC gene could regulate the expression of genes related to iron metabolism and have no effect on bacterial growth under the limited iron concentration. In the low concentration of Fe3+ and Fe2+ environment, SRW-OG1 can obtain iron ions by regulating yidC. Based on the above results, yidC gene contributed to the pathogenicity of mesophilic A. salmonicida to E. coioides, deletion of yidC gene promoted the inflammation and immune response of E. coioides to mesophilic A. salmonicida infection.
•The adhesion ability of ΔyidC strain decreased by about 83.36 % compared with that of the SRW-OG1 strain.•The SRW-OG1 strain ceased to grow when 300 μmol/L 2,2′-bipyridine was added to the culture medium.•In the low concentration of Fe3+ and Fe2+ environment, SRW-OG1 can obtain iron ions by regulating yidC.•The ΔyidC strain shows promising immune protection against Orange-spotted grouper (Epinephelus coioides). |
doi_str_mv | 10.1016/j.fsi.2024.109863 |
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•The adhesion ability of ΔyidC strain decreased by about 83.36 % compared with that of the SRW-OG1 strain.•The SRW-OG1 strain ceased to grow when 300 μmol/L 2,2′-bipyridine was added to the culture medium.•In the low concentration of Fe3+ and Fe2+ environment, SRW-OG1 can obtain iron ions by regulating yidC.•The ΔyidC strain shows promising immune protection against Orange-spotted grouper (Epinephelus coioides).</description><identifier>ISSN: 1050-4648</identifier><identifier>ISSN: 1095-9947</identifier><identifier>EISSN: 1095-9947</identifier><identifier>DOI: 10.1016/j.fsi.2024.109863</identifier><identifier>PMID: 39209005</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>adhesion ; Aeromonas salmonicida ; Aeromonas salmonicida - pathogenicity ; Aeromonas salmonicida - physiology ; Animals ; aquaculture industry ; bacterial growth ; Bacterial Proteins - genetics ; Bass - genetics ; Bass - immunology ; Epinephelus coioides ; Fish Diseases - immunology ; Fish Diseases - microbiology ; freshwater ; genes ; genetic engineering ; Gram-Negative Bacterial Infections - immunology ; Gram-Negative Bacterial Infections - microbiology ; Gram-Negative Bacterial Infections - veterinary ; immune response ; Immunity, Innate - genetics ; inflammation ; iron absorption ; marine fish ; Mesophilic Aeromonas salmonicida ; phenotype ; shellfish ; species ; survival rate ; Virulence ; Virulence regulation ; yidC</subject><ispartof>Fish & shellfish immunology, 2024-10, Vol.153, p.109863, Article 109863</ispartof><rights>2024</rights><rights>Copyright © 2024. Published by Elsevier Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0002-4704-4153</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1050464824005084$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39209005$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yi, Xin</creatorcontrib><creatorcontrib>Xu, XiaoJin</creatorcontrib><creatorcontrib>Xu, Genhuang</creatorcontrib><creatorcontrib>Zhang, Youyu</creatorcontrib><creatorcontrib>Chen, YuNong</creatorcontrib><creatorcontrib>Zhu, ZhiQin</creatorcontrib><creatorcontrib>Guo, Minglan</creatorcontrib><title>The Sec pathway gene yidC regulates the virulence of mesophilic Aeromonassalmonicida</title><title>Fish & shellfish immunology</title><addtitle>Fish Shellfish Immunol</addtitle><description>Aeromonas salmonicida is a common pathogenic bacterial species found in both freshwater and marine fish, leading to significant economic losses in the aquaculture industry. YidC is an accessory to SecYEG and is essential for the SecYEG transporter to insert into the bacterial membrane. However, the roles of the yidC gene on the host immune response remain unclear. Here, we compared the pathogenicity of yidC gene-deleted (ΔyidC) strain and wild-type (SRW-OG1) strain of mesophilic A. salmonicida to Orange-spotted grouper (Epinephelus coioides), and explored the impacts of yidC gene on the immune response of E. coioides to mesophilic A. salmonicida infection by using Red/ET recombineering. In this study, the E. coioides in the Secondary infected group had a 53.9 % higher survival rate than those in the Primary infected group. In addition, the adhesion ability of ΔyidC strain decreased by about 83.36 % compared with that of the wild-type (SRW-OG1) strain. Further comparison of the biological phenotype of SRW-OG1 and ΔyidC revealed that this yidC gene could regulate the expression of genes related to iron metabolism and have no effect on bacterial growth under the limited iron concentration. In the low concentration of Fe3+ and Fe2+ environment, SRW-OG1 can obtain iron ions by regulating yidC. Based on the above results, yidC gene contributed to the pathogenicity of mesophilic A. salmonicida to E. coioides, deletion of yidC gene promoted the inflammation and immune response of E. coioides to mesophilic A. salmonicida infection.
•The adhesion ability of ΔyidC strain decreased by about 83.36 % compared with that of the SRW-OG1 strain.•The SRW-OG1 strain ceased to grow when 300 μmol/L 2,2′-bipyridine was added to the culture medium.•In the low concentration of Fe3+ and Fe2+ environment, SRW-OG1 can obtain iron ions by regulating yidC.•The ΔyidC strain shows promising immune protection against Orange-spotted grouper (Epinephelus coioides).</description><subject>adhesion</subject><subject>Aeromonas salmonicida</subject><subject>Aeromonas salmonicida - pathogenicity</subject><subject>Aeromonas salmonicida - physiology</subject><subject>Animals</subject><subject>aquaculture industry</subject><subject>bacterial growth</subject><subject>Bacterial Proteins - genetics</subject><subject>Bass - genetics</subject><subject>Bass - immunology</subject><subject>Epinephelus coioides</subject><subject>Fish Diseases - immunology</subject><subject>Fish Diseases - microbiology</subject><subject>freshwater</subject><subject>genes</subject><subject>genetic engineering</subject><subject>Gram-Negative Bacterial Infections - immunology</subject><subject>Gram-Negative Bacterial Infections - microbiology</subject><subject>Gram-Negative Bacterial Infections - veterinary</subject><subject>immune response</subject><subject>Immunity, Innate - genetics</subject><subject>inflammation</subject><subject>iron absorption</subject><subject>marine fish</subject><subject>Mesophilic Aeromonas salmonicida</subject><subject>phenotype</subject><subject>shellfish</subject><subject>species</subject><subject>survival rate</subject><subject>Virulence</subject><subject>Virulence regulation</subject><subject>yidC</subject><issn>1050-4648</issn><issn>1095-9947</issn><issn>1095-9947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtPwzAQhC0EolD4AVyQj1xS7PgRW5yqipdUiQPlbDnOpnWVR4mTov57HLWcOc2s9tNKO4PQHSUzSqh83M7K4GcpSXmctZLsDF1FIxKteXY-ekESLrmaoOsQtoQQySS5RBOmU6IJEVdotdoA_gSHd7bf_NgDXkMD-OCLBe5gPVS2h4D7yOx9N1TQOMBtiWsI7W7jK-_wHLq2bhsbgq2ieucLe4MuSlsFuD3pFH29PK8Wb8ny4_V9MV8mQDkVSU5kxlmutUtZrmjqUuGkFjYtwbEyL4o0yyVwLbSTRDEmSqV5DoWLO0e4ZlP0cLy769rvAUJvah8cVJVtoB2CYVSwTHKt1P8o0VrFgJSI6P0JHfIaCrPrfG27g_kLLQJPRwDib3sPnQnOj9EUvgPXm6L1hhIzVmS2JlZkxorMsSL2CwiDgwM</recordid><startdate>202410</startdate><enddate>202410</enddate><creator>Yi, Xin</creator><creator>Xu, XiaoJin</creator><creator>Xu, Genhuang</creator><creator>Zhang, Youyu</creator><creator>Chen, YuNong</creator><creator>Zhu, ZhiQin</creator><creator>Guo, Minglan</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0002-4704-4153</orcidid></search><sort><creationdate>202410</creationdate><title>The Sec pathway gene yidC regulates the virulence of mesophilic Aeromonassalmonicida</title><author>Yi, Xin ; Xu, XiaoJin ; Xu, Genhuang ; Zhang, Youyu ; Chen, YuNong ; Zhu, ZhiQin ; Guo, Minglan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e1415-b06743b99c23b812c25c695a2fec3fbdd27b6e4959c608335f894bedcfbdc0493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>adhesion</topic><topic>Aeromonas salmonicida</topic><topic>Aeromonas salmonicida - pathogenicity</topic><topic>Aeromonas salmonicida - physiology</topic><topic>Animals</topic><topic>aquaculture industry</topic><topic>bacterial growth</topic><topic>Bacterial Proteins - genetics</topic><topic>Bass - genetics</topic><topic>Bass - immunology</topic><topic>Epinephelus coioides</topic><topic>Fish Diseases - immunology</topic><topic>Fish Diseases - microbiology</topic><topic>freshwater</topic><topic>genes</topic><topic>genetic engineering</topic><topic>Gram-Negative Bacterial Infections - immunology</topic><topic>Gram-Negative Bacterial Infections - microbiology</topic><topic>Gram-Negative Bacterial Infections - veterinary</topic><topic>immune response</topic><topic>Immunity, Innate - genetics</topic><topic>inflammation</topic><topic>iron absorption</topic><topic>marine fish</topic><topic>Mesophilic Aeromonas salmonicida</topic><topic>phenotype</topic><topic>shellfish</topic><topic>species</topic><topic>survival rate</topic><topic>Virulence</topic><topic>Virulence regulation</topic><topic>yidC</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yi, Xin</creatorcontrib><creatorcontrib>Xu, XiaoJin</creatorcontrib><creatorcontrib>Xu, Genhuang</creatorcontrib><creatorcontrib>Zhang, Youyu</creatorcontrib><creatorcontrib>Chen, YuNong</creatorcontrib><creatorcontrib>Zhu, ZhiQin</creatorcontrib><creatorcontrib>Guo, Minglan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Fish & shellfish immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yi, Xin</au><au>Xu, XiaoJin</au><au>Xu, Genhuang</au><au>Zhang, Youyu</au><au>Chen, YuNong</au><au>Zhu, ZhiQin</au><au>Guo, Minglan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Sec pathway gene yidC regulates the virulence of mesophilic Aeromonassalmonicida</atitle><jtitle>Fish & shellfish immunology</jtitle><addtitle>Fish Shellfish Immunol</addtitle><date>2024-10</date><risdate>2024</risdate><volume>153</volume><spage>109863</spage><pages>109863-</pages><artnum>109863</artnum><issn>1050-4648</issn><issn>1095-9947</issn><eissn>1095-9947</eissn><abstract>Aeromonas salmonicida is a common pathogenic bacterial species found in both freshwater and marine fish, leading to significant economic losses in the aquaculture industry. YidC is an accessory to SecYEG and is essential for the SecYEG transporter to insert into the bacterial membrane. However, the roles of the yidC gene on the host immune response remain unclear. Here, we compared the pathogenicity of yidC gene-deleted (ΔyidC) strain and wild-type (SRW-OG1) strain of mesophilic A. salmonicida to Orange-spotted grouper (Epinephelus coioides), and explored the impacts of yidC gene on the immune response of E. coioides to mesophilic A. salmonicida infection by using Red/ET recombineering. In this study, the E. coioides in the Secondary infected group had a 53.9 % higher survival rate than those in the Primary infected group. In addition, the adhesion ability of ΔyidC strain decreased by about 83.36 % compared with that of the wild-type (SRW-OG1) strain. Further comparison of the biological phenotype of SRW-OG1 and ΔyidC revealed that this yidC gene could regulate the expression of genes related to iron metabolism and have no effect on bacterial growth under the limited iron concentration. In the low concentration of Fe3+ and Fe2+ environment, SRW-OG1 can obtain iron ions by regulating yidC. Based on the above results, yidC gene contributed to the pathogenicity of mesophilic A. salmonicida to E. coioides, deletion of yidC gene promoted the inflammation and immune response of E. coioides to mesophilic A. salmonicida infection.
•The adhesion ability of ΔyidC strain decreased by about 83.36 % compared with that of the SRW-OG1 strain.•The SRW-OG1 strain ceased to grow when 300 μmol/L 2,2′-bipyridine was added to the culture medium.•In the low concentration of Fe3+ and Fe2+ environment, SRW-OG1 can obtain iron ions by regulating yidC.•The ΔyidC strain shows promising immune protection against Orange-spotted grouper (Epinephelus coioides).</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>39209005</pmid><doi>10.1016/j.fsi.2024.109863</doi><orcidid>https://orcid.org/0000-0002-4704-4153</orcidid></addata></record> |
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subjects | adhesion Aeromonas salmonicida Aeromonas salmonicida - pathogenicity Aeromonas salmonicida - physiology Animals aquaculture industry bacterial growth Bacterial Proteins - genetics Bass - genetics Bass - immunology Epinephelus coioides Fish Diseases - immunology Fish Diseases - microbiology freshwater genes genetic engineering Gram-Negative Bacterial Infections - immunology Gram-Negative Bacterial Infections - microbiology Gram-Negative Bacterial Infections - veterinary immune response Immunity, Innate - genetics inflammation iron absorption marine fish Mesophilic Aeromonas salmonicida phenotype shellfish species survival rate Virulence Virulence regulation yidC |
title | The Sec pathway gene yidC regulates the virulence of mesophilic Aeromonassalmonicida |
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