The Sec pathway gene yidC regulates the virulence of mesophilic Aeromonassalmonicida

Aeromonas salmonicida is a common pathogenic bacterial species found in both freshwater and marine fish, leading to significant economic losses in the aquaculture industry. YidC is an accessory to SecYEG and is essential for the SecYEG transporter to insert into the bacterial membrane. However, the...

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Veröffentlicht in:Fish & shellfish immunology 2024-10, Vol.153, p.109863, Article 109863
Hauptverfasser: Yi, Xin, Xu, XiaoJin, Xu, Genhuang, Zhang, Youyu, Chen, YuNong, Zhu, ZhiQin, Guo, Minglan
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container_start_page 109863
container_title Fish & shellfish immunology
container_volume 153
creator Yi, Xin
Xu, XiaoJin
Xu, Genhuang
Zhang, Youyu
Chen, YuNong
Zhu, ZhiQin
Guo, Minglan
description Aeromonas salmonicida is a common pathogenic bacterial species found in both freshwater and marine fish, leading to significant economic losses in the aquaculture industry. YidC is an accessory to SecYEG and is essential for the SecYEG transporter to insert into the bacterial membrane. However, the roles of the yidC gene on the host immune response remain unclear. Here, we compared the pathogenicity of yidC gene-deleted (ΔyidC) strain and wild-type (SRW-OG1) strain of mesophilic A. salmonicida to Orange-spotted grouper (Epinephelus coioides), and explored the impacts of yidC gene on the immune response of E. coioides to mesophilic A. salmonicida infection by using Red/ET recombineering. In this study, the E. coioides in the Secondary infected group had a 53.9 % higher survival rate than those in the Primary infected group. In addition, the adhesion ability of ΔyidC strain decreased by about 83.36 % compared with that of the wild-type (SRW-OG1) strain. Further comparison of the biological phenotype of SRW-OG1 and ΔyidC revealed that this yidC gene could regulate the expression of genes related to iron metabolism and have no effect on bacterial growth under the limited iron concentration. In the low concentration of Fe3+ and Fe2+ environment, SRW-OG1 can obtain iron ions by regulating yidC. Based on the above results, yidC gene contributed to the pathogenicity of mesophilic A. salmonicida to E. coioides, deletion of yidC gene promoted the inflammation and immune response of E. coioides to mesophilic A. salmonicida infection. •The adhesion ability of ΔyidC strain decreased by about 83.36 % compared with that of the SRW-OG1 strain.•The SRW-OG1 strain ceased to grow when 300 μmol/L 2,2′-bipyridine was added to the culture medium.•In the low concentration of Fe3+ and Fe2+ environment, SRW-OG1 can obtain iron ions by regulating yidC.•The ΔyidC strain shows promising immune protection against Orange-spotted grouper (Epinephelus coioides).
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YidC is an accessory to SecYEG and is essential for the SecYEG transporter to insert into the bacterial membrane. However, the roles of the yidC gene on the host immune response remain unclear. Here, we compared the pathogenicity of yidC gene-deleted (ΔyidC) strain and wild-type (SRW-OG1) strain of mesophilic A. salmonicida to Orange-spotted grouper (Epinephelus coioides), and explored the impacts of yidC gene on the immune response of E. coioides to mesophilic A. salmonicida infection by using Red/ET recombineering. In this study, the E. coioides in the Secondary infected group had a 53.9 % higher survival rate than those in the Primary infected group. In addition, the adhesion ability of ΔyidC strain decreased by about 83.36 % compared with that of the wild-type (SRW-OG1) strain. Further comparison of the biological phenotype of SRW-OG1 and ΔyidC revealed that this yidC gene could regulate the expression of genes related to iron metabolism and have no effect on bacterial growth under the limited iron concentration. In the low concentration of Fe3+ and Fe2+ environment, SRW-OG1 can obtain iron ions by regulating yidC. Based on the above results, yidC gene contributed to the pathogenicity of mesophilic A. salmonicida to E. coioides, deletion of yidC gene promoted the inflammation and immune response of E. coioides to mesophilic A. salmonicida infection. •The adhesion ability of ΔyidC strain decreased by about 83.36 % compared with that of the SRW-OG1 strain.•The SRW-OG1 strain ceased to grow when 300 μmol/L 2,2′-bipyridine was added to the culture medium.•In the low concentration of Fe3+ and Fe2+ environment, SRW-OG1 can obtain iron ions by regulating yidC.•The ΔyidC strain shows promising immune protection against Orange-spotted grouper (Epinephelus coioides).</description><identifier>ISSN: 1050-4648</identifier><identifier>ISSN: 1095-9947</identifier><identifier>EISSN: 1095-9947</identifier><identifier>DOI: 10.1016/j.fsi.2024.109863</identifier><identifier>PMID: 39209005</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>adhesion ; Aeromonas salmonicida ; Aeromonas salmonicida - pathogenicity ; Aeromonas salmonicida - physiology ; Animals ; aquaculture industry ; bacterial growth ; Bacterial Proteins - genetics ; Bass - genetics ; Bass - immunology ; Epinephelus coioides ; Fish Diseases - immunology ; Fish Diseases - microbiology ; freshwater ; genes ; genetic engineering ; Gram-Negative Bacterial Infections - immunology ; Gram-Negative Bacterial Infections - microbiology ; Gram-Negative Bacterial Infections - veterinary ; immune response ; Immunity, Innate - genetics ; inflammation ; iron absorption ; marine fish ; Mesophilic Aeromonas salmonicida ; phenotype ; shellfish ; species ; survival rate ; Virulence ; Virulence regulation ; yidC</subject><ispartof>Fish &amp; shellfish immunology, 2024-10, Vol.153, p.109863, Article 109863</ispartof><rights>2024</rights><rights>Copyright © 2024. 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YidC is an accessory to SecYEG and is essential for the SecYEG transporter to insert into the bacterial membrane. However, the roles of the yidC gene on the host immune response remain unclear. Here, we compared the pathogenicity of yidC gene-deleted (ΔyidC) strain and wild-type (SRW-OG1) strain of mesophilic A. salmonicida to Orange-spotted grouper (Epinephelus coioides), and explored the impacts of yidC gene on the immune response of E. coioides to mesophilic A. salmonicida infection by using Red/ET recombineering. In this study, the E. coioides in the Secondary infected group had a 53.9 % higher survival rate than those in the Primary infected group. In addition, the adhesion ability of ΔyidC strain decreased by about 83.36 % compared with that of the wild-type (SRW-OG1) strain. Further comparison of the biological phenotype of SRW-OG1 and ΔyidC revealed that this yidC gene could regulate the expression of genes related to iron metabolism and have no effect on bacterial growth under the limited iron concentration. In the low concentration of Fe3+ and Fe2+ environment, SRW-OG1 can obtain iron ions by regulating yidC. Based on the above results, yidC gene contributed to the pathogenicity of mesophilic A. salmonicida to E. coioides, deletion of yidC gene promoted the inflammation and immune response of E. coioides to mesophilic A. salmonicida infection. •The adhesion ability of ΔyidC strain decreased by about 83.36 % compared with that of the SRW-OG1 strain.•The SRW-OG1 strain ceased to grow when 300 μmol/L 2,2′-bipyridine was added to the culture medium.•In the low concentration of Fe3+ and Fe2+ environment, SRW-OG1 can obtain iron ions by regulating yidC.•The ΔyidC strain shows promising immune protection against Orange-spotted grouper (Epinephelus coioides).</description><subject>adhesion</subject><subject>Aeromonas salmonicida</subject><subject>Aeromonas salmonicida - pathogenicity</subject><subject>Aeromonas salmonicida - physiology</subject><subject>Animals</subject><subject>aquaculture industry</subject><subject>bacterial growth</subject><subject>Bacterial Proteins - genetics</subject><subject>Bass - genetics</subject><subject>Bass - immunology</subject><subject>Epinephelus coioides</subject><subject>Fish Diseases - immunology</subject><subject>Fish Diseases - microbiology</subject><subject>freshwater</subject><subject>genes</subject><subject>genetic engineering</subject><subject>Gram-Negative Bacterial Infections - immunology</subject><subject>Gram-Negative Bacterial Infections - microbiology</subject><subject>Gram-Negative Bacterial Infections - veterinary</subject><subject>immune response</subject><subject>Immunity, Innate - genetics</subject><subject>inflammation</subject><subject>iron absorption</subject><subject>marine fish</subject><subject>Mesophilic Aeromonas salmonicida</subject><subject>phenotype</subject><subject>shellfish</subject><subject>species</subject><subject>survival rate</subject><subject>Virulence</subject><subject>Virulence regulation</subject><subject>yidC</subject><issn>1050-4648</issn><issn>1095-9947</issn><issn>1095-9947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtPwzAQhC0EolD4AVyQj1xS7PgRW5yqipdUiQPlbDnOpnWVR4mTov57HLWcOc2s9tNKO4PQHSUzSqh83M7K4GcpSXmctZLsDF1FIxKteXY-ekESLrmaoOsQtoQQySS5RBOmU6IJEVdotdoA_gSHd7bf_NgDXkMD-OCLBe5gPVS2h4D7yOx9N1TQOMBtiWsI7W7jK-_wHLq2bhsbgq2ieucLe4MuSlsFuD3pFH29PK8Wb8ny4_V9MV8mQDkVSU5kxlmutUtZrmjqUuGkFjYtwbEyL4o0yyVwLbSTRDEmSqV5DoWLO0e4ZlP0cLy769rvAUJvah8cVJVtoB2CYVSwTHKt1P8o0VrFgJSI6P0JHfIaCrPrfG27g_kLLQJPRwDib3sPnQnOj9EUvgPXm6L1hhIzVmS2JlZkxorMsSL2CwiDgwM</recordid><startdate>202410</startdate><enddate>202410</enddate><creator>Yi, Xin</creator><creator>Xu, XiaoJin</creator><creator>Xu, Genhuang</creator><creator>Zhang, Youyu</creator><creator>Chen, YuNong</creator><creator>Zhu, ZhiQin</creator><creator>Guo, Minglan</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0002-4704-4153</orcidid></search><sort><creationdate>202410</creationdate><title>The Sec pathway gene yidC regulates the virulence of mesophilic Aeromonassalmonicida</title><author>Yi, Xin ; 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shellfish immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yi, Xin</au><au>Xu, XiaoJin</au><au>Xu, Genhuang</au><au>Zhang, Youyu</au><au>Chen, YuNong</au><au>Zhu, ZhiQin</au><au>Guo, Minglan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Sec pathway gene yidC regulates the virulence of mesophilic Aeromonassalmonicida</atitle><jtitle>Fish &amp; shellfish immunology</jtitle><addtitle>Fish Shellfish Immunol</addtitle><date>2024-10</date><risdate>2024</risdate><volume>153</volume><spage>109863</spage><pages>109863-</pages><artnum>109863</artnum><issn>1050-4648</issn><issn>1095-9947</issn><eissn>1095-9947</eissn><abstract>Aeromonas salmonicida is a common pathogenic bacterial species found in both freshwater and marine fish, leading to significant economic losses in the aquaculture industry. YidC is an accessory to SecYEG and is essential for the SecYEG transporter to insert into the bacterial membrane. However, the roles of the yidC gene on the host immune response remain unclear. Here, we compared the pathogenicity of yidC gene-deleted (ΔyidC) strain and wild-type (SRW-OG1) strain of mesophilic A. salmonicida to Orange-spotted grouper (Epinephelus coioides), and explored the impacts of yidC gene on the immune response of E. coioides to mesophilic A. salmonicida infection by using Red/ET recombineering. In this study, the E. coioides in the Secondary infected group had a 53.9 % higher survival rate than those in the Primary infected group. In addition, the adhesion ability of ΔyidC strain decreased by about 83.36 % compared with that of the wild-type (SRW-OG1) strain. Further comparison of the biological phenotype of SRW-OG1 and ΔyidC revealed that this yidC gene could regulate the expression of genes related to iron metabolism and have no effect on bacterial growth under the limited iron concentration. In the low concentration of Fe3+ and Fe2+ environment, SRW-OG1 can obtain iron ions by regulating yidC. Based on the above results, yidC gene contributed to the pathogenicity of mesophilic A. salmonicida to E. coioides, deletion of yidC gene promoted the inflammation and immune response of E. coioides to mesophilic A. salmonicida infection. •The adhesion ability of ΔyidC strain decreased by about 83.36 % compared with that of the SRW-OG1 strain.•The SRW-OG1 strain ceased to grow when 300 μmol/L 2,2′-bipyridine was added to the culture medium.•In the low concentration of Fe3+ and Fe2+ environment, SRW-OG1 can obtain iron ions by regulating yidC.•The ΔyidC strain shows promising immune protection against Orange-spotted grouper (Epinephelus coioides).</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>39209005</pmid><doi>10.1016/j.fsi.2024.109863</doi><orcidid>https://orcid.org/0000-0002-4704-4153</orcidid></addata></record>
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subjects adhesion
Aeromonas salmonicida
Aeromonas salmonicida - pathogenicity
Aeromonas salmonicida - physiology
Animals
aquaculture industry
bacterial growth
Bacterial Proteins - genetics
Bass - genetics
Bass - immunology
Epinephelus coioides
Fish Diseases - immunology
Fish Diseases - microbiology
freshwater
genes
genetic engineering
Gram-Negative Bacterial Infections - immunology
Gram-Negative Bacterial Infections - microbiology
Gram-Negative Bacterial Infections - veterinary
immune response
Immunity, Innate - genetics
inflammation
iron absorption
marine fish
Mesophilic Aeromonas salmonicida
phenotype
shellfish
species
survival rate
Virulence
Virulence regulation
yidC
title The Sec pathway gene yidC regulates the virulence of mesophilic Aeromonassalmonicida
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