Analysis and profiling of the purple acid phosphatase gene family in wheat (Triticum aestivum L.): Analysis and profiling of the purple acid phosphatase gene family in wheat (Triticum aestivum L.)
Purple acid phosphatases (PAPs) play a vital role in plant phosphorus nutrition, serving as a crucial family of metallo-phosphoesterase enzymes. This research aimed to identify the PAP genes from the A/B/D genomes of Triticum aestivum to elucidate evolutionary mechanisms of the gene family in plants...
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| description | Purple acid phosphatases (PAPs) play a vital role in plant phosphorus nutrition, serving as a crucial family of metallo-phosphoesterase enzymes. This research aimed to identify the PAP genes from the A/B/D genomes of
Triticum aestivum
to elucidate evolutionary mechanisms of the gene family in plants and provide genomic information for subsequent research on phosphorous-use efficiency in wheat crops. In total, 105 PAP genes (
TaPAP
s) were identified from the A/B/D genomes by using the
Arabidopsis thaliana
and
Oryza
sativa
PAP protein sequences as queries for BLASTP against the wheat protein database. The TaPAPs were grouped into six subfamilies, Ia (17), Ib (26), IIa (11), IIb (30), IIIa (12), and IIIb (9), based on their similarities in the structure of genes and the presence of conserved protein motifs. A majority of
TaPAP
s were derived from tandemly (20) or segmentally (87) duplicated, with the homoeologous chromosomes 5A/B/D harboring the most duplicated PAP genes. Further analysis indicated that
TaPAPs
were responsible for the modulation of seed, root, and leaf development and hormone synthesis and signaling, as well as plant responses to abiotic stresses, including low temperatures, drought, and anaerobic conditions. Nine
TaPAP
s (
TaPAP9-4A
/
4B
/
4D
,
TaPAP24-6A
/
6B
/
6D
, and
TaPAP28-7A
/
7B
/
7D
) were constitutively expressed in diverse tissues such as root, shoot, leaf, spike, and seed, while the remaining genes exhibited tissue-specific expression patterns. Concerning the response to phosphate (Pi) deprivation, 57
TaPAPs
were highly expressed in roots under Pi stress, including
TaPAP31-4A
,
4B
, and
4D
homeologs from the subfamily IIIb. A
TaPAP31-4A
transgene in
A. thaliana
promoted plant growth and development while increasing plant resistance to Pi-deficiency stress by enhancing the secretion of phosphatase. These discoveries provide a scientific foundation for comprehending the role of
TaPAPs
, offering valuable insights for identifying additional candidate genes and fostering the development of new wheat varieties with enhanced tolerance to low phosphorus conditions. |
| doi_str_mv | 10.1007/s00709-024-01983-6 |
| format | Article |
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Triticum aestivum
to elucidate evolutionary mechanisms of the gene family in plants and provide genomic information for subsequent research on phosphorous-use efficiency in wheat crops. In total, 105 PAP genes (
TaPAP
s) were identified from the A/B/D genomes by using the
Arabidopsis thaliana
and
Oryza
sativa
PAP protein sequences as queries for BLASTP against the wheat protein database. The TaPAPs were grouped into six subfamilies, Ia (17), Ib (26), IIa (11), IIb (30), IIIa (12), and IIIb (9), based on their similarities in the structure of genes and the presence of conserved protein motifs. A majority of
TaPAP
s were derived from tandemly (20) or segmentally (87) duplicated, with the homoeologous chromosomes 5A/B/D harboring the most duplicated PAP genes. Further analysis indicated that
TaPAPs
were responsible for the modulation of seed, root, and leaf development and hormone synthesis and signaling, as well as plant responses to abiotic stresses, including low temperatures, drought, and anaerobic conditions. Nine
TaPAP
s (
TaPAP9-4A
/
4B
/
4D
,
TaPAP24-6A
/
6B
/
6D
, and
TaPAP28-7A
/
7B
/
7D
) were constitutively expressed in diverse tissues such as root, shoot, leaf, spike, and seed, while the remaining genes exhibited tissue-specific expression patterns. Concerning the response to phosphate (Pi) deprivation, 57
TaPAPs
were highly expressed in roots under Pi stress, including
TaPAP31-4A
,
4B
, and
4D
homeologs from the subfamily IIIb. A
TaPAP31-4A
transgene in
A. thaliana
promoted plant growth and development while increasing plant resistance to Pi-deficiency stress by enhancing the secretion of phosphatase. These discoveries provide a scientific foundation for comprehending the role of
TaPAPs
, offering valuable insights for identifying additional candidate genes and fostering the development of new wheat varieties with enhanced tolerance to low phosphorus conditions.</description><identifier>ISSN: 0033-183X</identifier><identifier>ISSN: 1615-6102</identifier><identifier>EISSN: 1615-6102</identifier><identifier>DOI: 10.1007/s00709-024-01983-6</identifier><identifier>PMID: 39207505</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Acid Phosphatase - genetics ; Acid Phosphatase - metabolism ; Anaerobic conditions ; Biomedical and Life Sciences ; Cell Biology ; Drought ; Firing pattern ; Gene duplication ; Gene Expression Profiling ; Gene Expression Regulation, Plant ; Genes ; Genes, Plant ; Genomes ; Leaves ; Life Sciences ; Low temperature ; Multigene Family ; New varieties ; Nucleotide sequence ; Original Article ; Phosphatase ; Phosphoesterase ; Phosphorus ; Phylogeny ; Plant Proteins - chemistry ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant resistance ; Plant Sciences ; Protein structure ; Proteins ; Purple acid phosphatase ; Stress, Physiological - genetics ; Transgenes ; Transgenic plants ; Triticum - enzymology ; Triticum - genetics ; Triticum aestivum ; Wheat ; Zoology</subject><ispartof>Protoplasma, 2025, Vol.262 (1), p.73-86</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2024 Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.</rights><rights>Copyright Springer Nature B.V. Jan 2025</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c256t-b0f26e0ed42ca03e21266c3c86a3f7b0a60b138de19aed2787cf5cee3477f7ad3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00709-024-01983-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00709-024-01983-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39207505$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hou, Lijiang</creatorcontrib><creatorcontrib>Zhang, Dongzhi</creatorcontrib><creatorcontrib>Wu, Qiufang</creatorcontrib><creatorcontrib>Gao, Xinqiang</creatorcontrib><creatorcontrib>Wang, Junwei</creatorcontrib><title>Analysis and profiling of the purple acid phosphatase gene family in wheat (Triticum aestivum L.): Analysis and profiling of the purple acid phosphatase gene family in wheat (Triticum aestivum L.)</title><title>Protoplasma</title><addtitle>Protoplasma</addtitle><addtitle>Protoplasma</addtitle><description>Purple acid phosphatases (PAPs) play a vital role in plant phosphorus nutrition, serving as a crucial family of metallo-phosphoesterase enzymes. This research aimed to identify the PAP genes from the A/B/D genomes of
Triticum aestivum
to elucidate evolutionary mechanisms of the gene family in plants and provide genomic information for subsequent research on phosphorous-use efficiency in wheat crops. In total, 105 PAP genes (
TaPAP
s) were identified from the A/B/D genomes by using the
Arabidopsis thaliana
and
Oryza
sativa
PAP protein sequences as queries for BLASTP against the wheat protein database. The TaPAPs were grouped into six subfamilies, Ia (17), Ib (26), IIa (11), IIb (30), IIIa (12), and IIIb (9), based on their similarities in the structure of genes and the presence of conserved protein motifs. A majority of
TaPAP
s were derived from tandemly (20) or segmentally (87) duplicated, with the homoeologous chromosomes 5A/B/D harboring the most duplicated PAP genes. Further analysis indicated that
TaPAPs
were responsible for the modulation of seed, root, and leaf development and hormone synthesis and signaling, as well as plant responses to abiotic stresses, including low temperatures, drought, and anaerobic conditions. Nine
TaPAP
s (
TaPAP9-4A
/
4B
/
4D
,
TaPAP24-6A
/
6B
/
6D
, and
TaPAP28-7A
/
7B
/
7D
) were constitutively expressed in diverse tissues such as root, shoot, leaf, spike, and seed, while the remaining genes exhibited tissue-specific expression patterns. Concerning the response to phosphate (Pi) deprivation, 57
TaPAPs
were highly expressed in roots under Pi stress, including
TaPAP31-4A
,
4B
, and
4D
homeologs from the subfamily IIIb. A
TaPAP31-4A
transgene in
A. thaliana
promoted plant growth and development while increasing plant resistance to Pi-deficiency stress by enhancing the secretion of phosphatase. These discoveries provide a scientific foundation for comprehending the role of
TaPAPs
, offering valuable insights for identifying additional candidate genes and fostering the development of new wheat varieties with enhanced tolerance to low phosphorus conditions.</description><subject>Acid Phosphatase - genetics</subject><subject>Acid Phosphatase - metabolism</subject><subject>Anaerobic conditions</subject><subject>Biomedical and Life Sciences</subject><subject>Cell Biology</subject><subject>Drought</subject><subject>Firing pattern</subject><subject>Gene duplication</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes</subject><subject>Genes, Plant</subject><subject>Genomes</subject><subject>Leaves</subject><subject>Life Sciences</subject><subject>Low temperature</subject><subject>Multigene Family</subject><subject>New varieties</subject><subject>Nucleotide sequence</subject><subject>Original Article</subject><subject>Phosphatase</subject><subject>Phosphoesterase</subject><subject>Phosphorus</subject><subject>Phylogeny</subject><subject>Plant Proteins - chemistry</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plant resistance</subject><subject>Plant Sciences</subject><subject>Protein structure</subject><subject>Proteins</subject><subject>Purple acid phosphatase</subject><subject>Stress, Physiological - genetics</subject><subject>Transgenes</subject><subject>Transgenic plants</subject><subject>Triticum - enzymology</subject><subject>Triticum - genetics</subject><subject>Triticum aestivum</subject><subject>Wheat</subject><subject>Zoology</subject><issn>0033-183X</issn><issn>1615-6102</issn><issn>1615-6102</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2025</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU9P3DAQxa2qqCyUL9BDZakXOATG9sZOjgi1gLRSLyD1Zs06412j_KudUO23r8tCkThw8Via3zx73mPsi4BzAWAuUj6gLkAuCxB1pQr9gS2EFmWhBciPbAGgVCEq9euQHaX0AAClhPITO1S1BFNCuWB42WO7SyFx7Bs-xsGHNvQbPng-bYmPcxxb4uhCbm6HNG5xwkR8Qz1xj11odzz0_M-WcOKndzFMwc0dR0pTeMyX1fnZZ3bgsU108lyP2f2P73dXN8Xq5_Xt1eWqcLLUU7EGLzUBNUvpEBRJIbV2ylUalTdrQA1roaqGRI3USFMZ50tHpJbGeIONOmane928xO85f8B2ITlqW-xpmJNVUNemNtJARr-9QR-GOWYjMiVKobJNtcmU3FMuDilF8naMocO4swLsvwDsPgCbA7BPAVidh74-S8_rjpr_Iy-OZ0DtgZRb_Ybi69vvyP4F8BWQgw</recordid><startdate>2025</startdate><enddate>2025</enddate><creator>Hou, Lijiang</creator><creator>Zhang, Dongzhi</creator><creator>Wu, Qiufang</creator><creator>Gao, Xinqiang</creator><creator>Wang, Junwei</creator><general>Springer Vienna</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7X8</scope></search><sort><creationdate>2025</creationdate><title>Analysis and profiling of the purple acid phosphatase gene family in wheat (Triticum aestivum L.)</title><author>Hou, Lijiang ; Zhang, Dongzhi ; Wu, Qiufang ; Gao, Xinqiang ; Wang, Junwei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c256t-b0f26e0ed42ca03e21266c3c86a3f7b0a60b138de19aed2787cf5cee3477f7ad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2025</creationdate><topic>Acid Phosphatase - genetics</topic><topic>Acid Phosphatase - metabolism</topic><topic>Anaerobic conditions</topic><topic>Biomedical and Life Sciences</topic><topic>Cell Biology</topic><topic>Drought</topic><topic>Firing pattern</topic><topic>Gene duplication</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genes</topic><topic>Genes, Plant</topic><topic>Genomes</topic><topic>Leaves</topic><topic>Life Sciences</topic><topic>Low temperature</topic><topic>Multigene Family</topic><topic>New varieties</topic><topic>Nucleotide sequence</topic><topic>Original Article</topic><topic>Phosphatase</topic><topic>Phosphoesterase</topic><topic>Phosphorus</topic><topic>Phylogeny</topic><topic>Plant Proteins - chemistry</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plant resistance</topic><topic>Plant Sciences</topic><topic>Protein structure</topic><topic>Proteins</topic><topic>Purple acid phosphatase</topic><topic>Stress, Physiological - genetics</topic><topic>Transgenes</topic><topic>Transgenic plants</topic><topic>Triticum - enzymology</topic><topic>Triticum - genetics</topic><topic>Triticum aestivum</topic><topic>Wheat</topic><topic>Zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hou, Lijiang</creatorcontrib><creatorcontrib>Zhang, Dongzhi</creatorcontrib><creatorcontrib>Wu, Qiufang</creatorcontrib><creatorcontrib>Gao, Xinqiang</creatorcontrib><creatorcontrib>Wang, Junwei</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><jtitle>Protoplasma</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hou, Lijiang</au><au>Zhang, Dongzhi</au><au>Wu, Qiufang</au><au>Gao, Xinqiang</au><au>Wang, Junwei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis and profiling of the purple acid phosphatase gene family in wheat (Triticum aestivum L.): Analysis and profiling of the purple acid phosphatase gene family in wheat (Triticum aestivum L.)</atitle><jtitle>Protoplasma</jtitle><stitle>Protoplasma</stitle><addtitle>Protoplasma</addtitle><date>2025</date><risdate>2025</risdate><volume>262</volume><issue>1</issue><spage>73</spage><epage>86</epage><pages>73-86</pages><issn>0033-183X</issn><issn>1615-6102</issn><eissn>1615-6102</eissn><abstract>Purple acid phosphatases (PAPs) play a vital role in plant phosphorus nutrition, serving as a crucial family of metallo-phosphoesterase enzymes. This research aimed to identify the PAP genes from the A/B/D genomes of
Triticum aestivum
to elucidate evolutionary mechanisms of the gene family in plants and provide genomic information for subsequent research on phosphorous-use efficiency in wheat crops. In total, 105 PAP genes (
TaPAP
s) were identified from the A/B/D genomes by using the
Arabidopsis thaliana
and
Oryza
sativa
PAP protein sequences as queries for BLASTP against the wheat protein database. The TaPAPs were grouped into six subfamilies, Ia (17), Ib (26), IIa (11), IIb (30), IIIa (12), and IIIb (9), based on their similarities in the structure of genes and the presence of conserved protein motifs. A majority of
TaPAP
s were derived from tandemly (20) or segmentally (87) duplicated, with the homoeologous chromosomes 5A/B/D harboring the most duplicated PAP genes. Further analysis indicated that
TaPAPs
were responsible for the modulation of seed, root, and leaf development and hormone synthesis and signaling, as well as plant responses to abiotic stresses, including low temperatures, drought, and anaerobic conditions. Nine
TaPAP
s (
TaPAP9-4A
/
4B
/
4D
,
TaPAP24-6A
/
6B
/
6D
, and
TaPAP28-7A
/
7B
/
7D
) were constitutively expressed in diverse tissues such as root, shoot, leaf, spike, and seed, while the remaining genes exhibited tissue-specific expression patterns. Concerning the response to phosphate (Pi) deprivation, 57
TaPAPs
were highly expressed in roots under Pi stress, including
TaPAP31-4A
,
4B
, and
4D
homeologs from the subfamily IIIb. A
TaPAP31-4A
transgene in
A. thaliana
promoted plant growth and development while increasing plant resistance to Pi-deficiency stress by enhancing the secretion of phosphatase. These discoveries provide a scientific foundation for comprehending the role of
TaPAPs
, offering valuable insights for identifying additional candidate genes and fostering the development of new wheat varieties with enhanced tolerance to low phosphorus conditions.</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><pmid>39207505</pmid><doi>10.1007/s00709-024-01983-6</doi><tpages>14</tpages></addata></record> |
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| source | MEDLINE; Springer Nature |
| subjects | Acid Phosphatase - genetics Acid Phosphatase - metabolism Anaerobic conditions Biomedical and Life Sciences Cell Biology Drought Firing pattern Gene duplication Gene Expression Profiling Gene Expression Regulation, Plant Genes Genes, Plant Genomes Leaves Life Sciences Low temperature Multigene Family New varieties Nucleotide sequence Original Article Phosphatase Phosphoesterase Phosphorus Phylogeny Plant Proteins - chemistry Plant Proteins - genetics Plant Proteins - metabolism Plant resistance Plant Sciences Protein structure Proteins Purple acid phosphatase Stress, Physiological - genetics Transgenes Transgenic plants Triticum - enzymology Triticum - genetics Triticum aestivum Wheat Zoology |
| title | Analysis and profiling of the purple acid phosphatase gene family in wheat (Triticum aestivum L.): Analysis and profiling of the purple acid phosphatase gene family in wheat (Triticum aestivum L.) |
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