One‐pot derivatization spectrofluorimetric method for detection of aspartame in sweetener commercial tablets and soft drinks: Greenness assessments of the methodology
Aspartame is an artificial sweetener used in drinks and many foods. International Agency for Research on Cancer classified aspartame as possibly carcinogenic to humans (IARC Group 2B). In this study, a sensitive and selective spectrofluorimetric method was developed to detect aspartame. The method i...
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| Veröffentlicht in: | Luminescence (Chichester, England) England), 2024-09, Vol.39 (9), p.e4882-n/a |
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| creator | AlSalem, Huda Salem Alharbi, Sara Naif Binkadem, Mona Saad Katamesh, Noha S. Abdel‐Lateef, Mohamed A. |
| description | Aspartame is an artificial sweetener used in drinks and many foods. International Agency for Research on Cancer classified aspartame as possibly carcinogenic to humans (IARC Group 2B). In this study, a sensitive and selective spectrofluorimetric method was developed to detect aspartame. The method is based on switching on the fluorescence activity of aspartame upon its condensation with O‐phthalaldehyde (Roth's reaction) in the presence of 2‐mercaptoethanol. The reaction product was detected fluorometrically at λem of 438 nm after λex of 340 nm. All reaction conditions required to yield the optimal fluorescence intensity were observed and investigated. Furthermore, the approach was validated according to ICH guidelines. Upon plotting the concentrations of aspartame against their associated fluorescence intensity values, the relationship between the two variables was linear within the range of 0.5–3.0 μg/mL. Furthermore, the method was employed to analyze the quantity of aspartame in commercial packages and soft drinks with an acceptable level of recovery. In addition, the Green Solvents Selecting Tool, Complementary Green Analytical Procedure Index, and the Analytical Greenness Metric tool were used to evaluate the sustainability and the greenness of the developed methodology.
A spectrofluorimetric method was developed to determine aspartame based on Roth's reaction.
Aspartame has been detected on tablets and soft drinks samples.
Various tools were used to evaluate the sustainability and greenness of the proposed method. |
| doi_str_mv | 10.1002/bio.4882 |
| format | Article |
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A spectrofluorimetric method was developed to determine aspartame based on Roth's reaction.
Aspartame has been detected on tablets and soft drinks samples.
Various tools were used to evaluate the sustainability and greenness of the proposed method.</description><identifier>ISSN: 1522-7235</identifier><identifier>ISSN: 1522-7243</identifier><identifier>EISSN: 1522-7243</identifier><identifier>DOI: 10.1002/bio.4882</identifier><identifier>PMID: 39192838</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Aspartame ; Aspartame - analysis ; Beverages ; Carbonated Beverages - analysis ; Carcinogens ; Condensates ; Fluorescence ; food analysis ; Green Chemistry Technology ; Mercaptoethanol - chemistry ; o-Phthalaldehyde - chemistry ; O‐phthalaldehyde ; Reaction products ; Roth's reaction ; Soft drinks ; Spectrofluorimetry ; spectrofluorometric assay ; Spectrometry, Fluorescence - methods ; Sweetening Agents - analysis ; Tablets - analysis</subject><ispartof>Luminescence (Chichester, England), 2024-09, Vol.39 (9), p.e4882-n/a</ispartof><rights>2024 John Wiley & Sons Ltd.</rights><rights>2024 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c2402-9ccd1c904631ccc1fc7b59773a3e15acff332b57e880b0cfe3723c59a003b4353</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbio.4882$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbio.4882$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39192838$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>AlSalem, Huda Salem</creatorcontrib><creatorcontrib>Alharbi, Sara Naif</creatorcontrib><creatorcontrib>Binkadem, Mona Saad</creatorcontrib><creatorcontrib>Katamesh, Noha S.</creatorcontrib><creatorcontrib>Abdel‐Lateef, Mohamed A.</creatorcontrib><title>One‐pot derivatization spectrofluorimetric method for detection of aspartame in sweetener commercial tablets and soft drinks: Greenness assessments of the methodology</title><title>Luminescence (Chichester, England)</title><addtitle>Luminescence</addtitle><description>Aspartame is an artificial sweetener used in drinks and many foods. International Agency for Research on Cancer classified aspartame as possibly carcinogenic to humans (IARC Group 2B). In this study, a sensitive and selective spectrofluorimetric method was developed to detect aspartame. The method is based on switching on the fluorescence activity of aspartame upon its condensation with O‐phthalaldehyde (Roth's reaction) in the presence of 2‐mercaptoethanol. The reaction product was detected fluorometrically at λem of 438 nm after λex of 340 nm. All reaction conditions required to yield the optimal fluorescence intensity were observed and investigated. Furthermore, the approach was validated according to ICH guidelines. Upon plotting the concentrations of aspartame against their associated fluorescence intensity values, the relationship between the two variables was linear within the range of 0.5–3.0 μg/mL. Furthermore, the method was employed to analyze the quantity of aspartame in commercial packages and soft drinks with an acceptable level of recovery. In addition, the Green Solvents Selecting Tool, Complementary Green Analytical Procedure Index, and the Analytical Greenness Metric tool were used to evaluate the sustainability and the greenness of the developed methodology.
A spectrofluorimetric method was developed to determine aspartame based on Roth's reaction.
Aspartame has been detected on tablets and soft drinks samples.
Various tools were used to evaluate the sustainability and greenness of the proposed method.</description><subject>Aspartame</subject><subject>Aspartame - analysis</subject><subject>Beverages</subject><subject>Carbonated Beverages - analysis</subject><subject>Carcinogens</subject><subject>Condensates</subject><subject>Fluorescence</subject><subject>food analysis</subject><subject>Green Chemistry Technology</subject><subject>Mercaptoethanol - chemistry</subject><subject>o-Phthalaldehyde - chemistry</subject><subject>O‐phthalaldehyde</subject><subject>Reaction products</subject><subject>Roth's reaction</subject><subject>Soft drinks</subject><subject>Spectrofluorimetry</subject><subject>spectrofluorometric assay</subject><subject>Spectrometry, Fluorescence - methods</subject><subject>Sweetening Agents - analysis</subject><subject>Tablets - analysis</subject><issn>1522-7235</issn><issn>1522-7243</issn><issn>1522-7243</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kU1u1TAUhSMEoqUgsQJkiQmTFP_EScwMqlIqVXoTGFuOc01dEjvYTqvHiCV0GayLlXAffRQJiYF1LJ3P51r3VNVzRo8Zpfz14ONx0_f8QXXIJOd1xxvx8P4u5EH1JOcrSmnbtupxdSAUU7wX_WH1YxPg5_fbJRYyQvLXpvhveGIgeQFbUnTTGpOfoSRvCcplHImLCemC_g6Mjpi8mFTMDMTjwxtAL0AiNs4zJOvNRIoZJiiZmDCSHB1OSz58yW_IWQIIATJaOaPMEBDDzHIJ-3lxip-3T6tHzkwZnu31qPr0_vTjyYf6YnN2fvL2ora8obxW1o7MKtq0gllrmbPdIFXXCSOASWOdE4IPsoO-pwO1DgTux0plKBVDI6Q4ql7d5S4pfl0hFz37bGGaTIC4Zi2o6nopmpYj-vIf9CquKeDvtGBUyZb2gv0NtCnmnMDpBfdp0lYzqnftaWxP79pD9MU-cB1mGO_BP3UhUN8BN36C7X-D9Lvzze_AX7vJqLQ</recordid><startdate>202409</startdate><enddate>202409</enddate><creator>AlSalem, Huda Salem</creator><creator>Alharbi, Sara Naif</creator><creator>Binkadem, Mona Saad</creator><creator>Katamesh, Noha S.</creator><creator>Abdel‐Lateef, Mohamed A.</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QP</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>7U7</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H95</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L.G</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>202409</creationdate><title>One‐pot derivatization spectrofluorimetric method for detection of aspartame in sweetener commercial tablets and soft drinks: Greenness assessments of the methodology</title><author>AlSalem, Huda Salem ; 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International Agency for Research on Cancer classified aspartame as possibly carcinogenic to humans (IARC Group 2B). In this study, a sensitive and selective spectrofluorimetric method was developed to detect aspartame. The method is based on switching on the fluorescence activity of aspartame upon its condensation with O‐phthalaldehyde (Roth's reaction) in the presence of 2‐mercaptoethanol. The reaction product was detected fluorometrically at λem of 438 nm after λex of 340 nm. All reaction conditions required to yield the optimal fluorescence intensity were observed and investigated. Furthermore, the approach was validated according to ICH guidelines. Upon plotting the concentrations of aspartame against their associated fluorescence intensity values, the relationship between the two variables was linear within the range of 0.5–3.0 μg/mL. Furthermore, the method was employed to analyze the quantity of aspartame in commercial packages and soft drinks with an acceptable level of recovery. In addition, the Green Solvents Selecting Tool, Complementary Green Analytical Procedure Index, and the Analytical Greenness Metric tool were used to evaluate the sustainability and the greenness of the developed methodology.
A spectrofluorimetric method was developed to determine aspartame based on Roth's reaction.
Aspartame has been detected on tablets and soft drinks samples.
Various tools were used to evaluate the sustainability and greenness of the proposed method.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>39192838</pmid><doi>10.1002/bio.4882</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1522-7235 |
| ispartof | Luminescence (Chichester, England), 2024-09, Vol.39 (9), p.e4882-n/a |
| issn | 1522-7235 1522-7243 1522-7243 |
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| source | MEDLINE; Wiley Online Library All Journals |
| subjects | Aspartame Aspartame - analysis Beverages Carbonated Beverages - analysis Carcinogens Condensates Fluorescence food analysis Green Chemistry Technology Mercaptoethanol - chemistry o-Phthalaldehyde - chemistry O‐phthalaldehyde Reaction products Roth's reaction Soft drinks Spectrofluorimetry spectrofluorometric assay Spectrometry, Fluorescence - methods Sweetening Agents - analysis Tablets - analysis |
| title | One‐pot derivatization spectrofluorimetric method for detection of aspartame in sweetener commercial tablets and soft drinks: Greenness assessments of the methodology |
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