2-DG improves lung ischemia/reperfusion injury by inhibiting NLRP3-mediated pyroptosis in rats
The aim of this study was to investigate whether the protective effect of 2-deoxyglucose (2-DG) on lung ischemia/reperfusion (I/R) injury is mediated by inhibiting nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3)-mediated pyroptosis in rats. Male Sprague-Dawley rats were ran...
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| Veröffentlicht in: | Sheng li hsüeh pao 2024-08, Vol.76 (4), p.517 |
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| container_title | Sheng li hsüeh pao |
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| creator | Shi, Lu Huang, Man Chen, Si-An Xu, Jun-Peng Zhang, Qi-Hao Cao, Wen-Jie Tian, Yun-Na Wang, Xiao-Ting Wang, Wan-Tie |
| description | The aim of this study was to investigate whether the protective effect of 2-deoxyglucose (2-DG) on lung ischemia/reperfusion (I/R) injury is mediated by inhibiting nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3)-mediated pyroptosis in rats. Male Sprague-Dawley rats were randomly divided into control group, 2-DG group, lung I/R injury group (I/R group) and 2-DG+I/R group. 2-DG (0.7 g/kg) was intraperitoneally injected 1 h prior to lung ischemia. The tissue structure was measured under light microscope. Lung injury parameters were detected. The contents of malondialdehyde (MDA), myeloperoxidase (MPO) and lactate were determined by commercially available kits. ELISA was used to detect the levels of IL-1β and IL-18. Western blot, qRT-PCR and immunofluorescence staining were used to measure the expression changes of glycolysis and pyroptosis related indicators. The results showed that there was no significant difference in the parameters between the control group and the 2-DG group. Howev |
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Male Sprague-Dawley rats were randomly divided into control group, 2-DG group, lung I/R injury group (I/R group) and 2-DG+I/R group. 2-DG (0.7 g/kg) was intraperitoneally injected 1 h prior to lung ischemia. The tissue structure was measured under light microscope. Lung injury parameters were detected. The contents of malondialdehyde (MDA), myeloperoxidase (MPO) and lactate were determined by commercially available kits. ELISA was used to detect the levels of IL-1β and IL-18. Western blot, qRT-PCR and immunofluorescence staining were used to measure the expression changes of glycolysis and pyroptosis related indicators. The results showed that there was no significant difference in the parameters between the control group and the 2-DG group. Howev</description><identifier>ISSN: 0371-0874</identifier><identifier>PMID: 39192785</identifier><language>chi</language><publisher>China</publisher><subject>Animals ; Deoxyglucose - pharmacology ; Interleukin-18 - metabolism ; Interleukin-1beta - metabolism ; Lung - metabolism ; Lung - pathology ; Lung Injury - etiology ; Lung Injury - metabolism ; Lung Injury - prevention & control ; Male ; NLR Family, Pyrin Domain-Containing 3 Protein - metabolism ; Oxidative Stress ; Pyroptosis ; Rats ; Rats, Sprague-Dawley ; Reperfusion Injury - metabolism ; Reperfusion Injury - prevention & control</subject><ispartof>Sheng li hsüeh pao, 2024-08, Vol.76 (4), p.517</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39192785$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shi, Lu</creatorcontrib><creatorcontrib>Huang, Man</creatorcontrib><creatorcontrib>Chen, Si-An</creatorcontrib><creatorcontrib>Xu, Jun-Peng</creatorcontrib><creatorcontrib>Zhang, Qi-Hao</creatorcontrib><creatorcontrib>Cao, Wen-Jie</creatorcontrib><creatorcontrib>Tian, Yun-Na</creatorcontrib><creatorcontrib>Wang, Xiao-Ting</creatorcontrib><creatorcontrib>Wang, Wan-Tie</creatorcontrib><title>2-DG improves lung ischemia/reperfusion injury by inhibiting NLRP3-mediated pyroptosis in rats</title><title>Sheng li hsüeh pao</title><addtitle>Sheng Li Xue Bao</addtitle><description>The aim of this study was to investigate whether the protective effect of 2-deoxyglucose (2-DG) on lung ischemia/reperfusion (I/R) injury is mediated by inhibiting nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3)-mediated pyroptosis in rats. Male Sprague-Dawley rats were randomly divided into control group, 2-DG group, lung I/R injury group (I/R group) and 2-DG+I/R group. 2-DG (0.7 g/kg) was intraperitoneally injected 1 h prior to lung ischemia. The tissue structure was measured under light microscope. Lung injury parameters were detected. The contents of malondialdehyde (MDA), myeloperoxidase (MPO) and lactate were determined by commercially available kits. ELISA was used to detect the levels of IL-1β and IL-18. Western blot, qRT-PCR and immunofluorescence staining were used to measure the expression changes of glycolysis and pyroptosis related indicators. The results showed that there was no significant difference in the parameters between the control group and the 2-DG group. Howev</description><subject>Animals</subject><subject>Deoxyglucose - pharmacology</subject><subject>Interleukin-18 - metabolism</subject><subject>Interleukin-1beta - metabolism</subject><subject>Lung - metabolism</subject><subject>Lung - pathology</subject><subject>Lung Injury - etiology</subject><subject>Lung Injury - metabolism</subject><subject>Lung Injury - prevention & control</subject><subject>Male</subject><subject>NLR Family, Pyrin Domain-Containing 3 Protein - metabolism</subject><subject>Oxidative Stress</subject><subject>Pyroptosis</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reperfusion Injury - metabolism</subject><subject>Reperfusion Injury - prevention & control</subject><issn>0371-0874</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kEtLxDAUhbNQnGGcvyBZugmmeTTNUkYdB4qKzNqSNLdOpC-TVui_N-C4OmfxcbnfuUBrylVGaKHECm1j9JbKjCshCnWFVlxnmqlCrtEHIw977LsxDD8QcTv3n9jH-gSdN3cBRgjNHP3QY99_zWHBdknt5K2ffCJfyvc3Tjpw3kzg8LiEYZyG6GOCcDBTvEaXjWkjbM-5Qcenx-PumZSv-8PuviSjzCXJGFW55AKAaiGzxjlZZ9oyyajTDlRRK8eZsKqWTNsUOteikQU1stFSW75Bt39nk8b3DHGquiQBbWt6GOZYcaqTLdOUJvTmjM42PV6NwXcmLNX_JPwXdpNc-A</recordid><startdate>20240825</startdate><enddate>20240825</enddate><creator>Shi, Lu</creator><creator>Huang, Man</creator><creator>Chen, Si-An</creator><creator>Xu, Jun-Peng</creator><creator>Zhang, Qi-Hao</creator><creator>Cao, Wen-Jie</creator><creator>Tian, Yun-Na</creator><creator>Wang, Xiao-Ting</creator><creator>Wang, Wan-Tie</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20240825</creationdate><title>2-DG improves lung ischemia/reperfusion injury by inhibiting NLRP3-mediated pyroptosis in rats</title><author>Shi, Lu ; Huang, Man ; Chen, Si-An ; Xu, Jun-Peng ; Zhang, Qi-Hao ; Cao, Wen-Jie ; Tian, Yun-Na ; Wang, Xiao-Ting ; Wang, Wan-Tie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p565-12076534ee09451fdd5c19b2520d9de78c7d324b7c529bb7c9694f580a5f959b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2024</creationdate><topic>Animals</topic><topic>Deoxyglucose - pharmacology</topic><topic>Interleukin-18 - metabolism</topic><topic>Interleukin-1beta - metabolism</topic><topic>Lung - metabolism</topic><topic>Lung - pathology</topic><topic>Lung Injury - etiology</topic><topic>Lung Injury - metabolism</topic><topic>Lung Injury - prevention & control</topic><topic>Male</topic><topic>NLR Family, Pyrin Domain-Containing 3 Protein - metabolism</topic><topic>Oxidative Stress</topic><topic>Pyroptosis</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Reperfusion Injury - metabolism</topic><topic>Reperfusion Injury - prevention & control</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shi, Lu</creatorcontrib><creatorcontrib>Huang, Man</creatorcontrib><creatorcontrib>Chen, Si-An</creatorcontrib><creatorcontrib>Xu, Jun-Peng</creatorcontrib><creatorcontrib>Zhang, Qi-Hao</creatorcontrib><creatorcontrib>Cao, Wen-Jie</creatorcontrib><creatorcontrib>Tian, Yun-Na</creatorcontrib><creatorcontrib>Wang, Xiao-Ting</creatorcontrib><creatorcontrib>Wang, Wan-Tie</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Sheng li hsüeh pao</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shi, Lu</au><au>Huang, Man</au><au>Chen, Si-An</au><au>Xu, Jun-Peng</au><au>Zhang, Qi-Hao</au><au>Cao, Wen-Jie</au><au>Tian, Yun-Na</au><au>Wang, Xiao-Ting</au><au>Wang, Wan-Tie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>2-DG improves lung ischemia/reperfusion injury by inhibiting NLRP3-mediated pyroptosis in rats</atitle><jtitle>Sheng li hsüeh pao</jtitle><addtitle>Sheng Li Xue Bao</addtitle><date>2024-08-25</date><risdate>2024</risdate><volume>76</volume><issue>4</issue><spage>517</spage><pages>517-</pages><issn>0371-0874</issn><abstract>The aim of this study was to investigate whether the protective effect of 2-deoxyglucose (2-DG) on lung ischemia/reperfusion (I/R) injury is mediated by inhibiting nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3)-mediated pyroptosis in rats. Male Sprague-Dawley rats were randomly divided into control group, 2-DG group, lung I/R injury group (I/R group) and 2-DG+I/R group. 2-DG (0.7 g/kg) was intraperitoneally injected 1 h prior to lung ischemia. The tissue structure was measured under light microscope. Lung injury parameters were detected. The contents of malondialdehyde (MDA), myeloperoxidase (MPO) and lactate were determined by commercially available kits. ELISA was used to detect the levels of IL-1β and IL-18. Western blot, qRT-PCR and immunofluorescence staining were used to measure the expression changes of glycolysis and pyroptosis related indicators. The results showed that there was no significant difference in the parameters between the control group and the 2-DG group. Howev</abstract><cop>China</cop><pmid>39192785</pmid></addata></record> |
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| subjects | Animals Deoxyglucose - pharmacology Interleukin-18 - metabolism Interleukin-1beta - metabolism Lung - metabolism Lung - pathology Lung Injury - etiology Lung Injury - metabolism Lung Injury - prevention & control Male NLR Family, Pyrin Domain-Containing 3 Protein - metabolism Oxidative Stress Pyroptosis Rats Rats, Sprague-Dawley Reperfusion Injury - metabolism Reperfusion Injury - prevention & control |
| title | 2-DG improves lung ischemia/reperfusion injury by inhibiting NLRP3-mediated pyroptosis in rats |
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