Essential oils of Eugenia spp. (myrtaceae) show in vitro antibacterial activity against Staphylococcus aureus isolates from bovine mastitis
Highlights • The essential oils of the genus Eugenia show significant antibacterial activity. • Essential oils combat the formation of Staphylococcus aureus biofilm responsible for bovine mastitis. • The essential oils of the genus Eugenia act synergistically with oxacillin. • The essential oil of E...
Gespeichert in:
| Veröffentlicht in: | Brazilian journal of microbiology 2024-12, Vol.55 (4), p.3081-3096 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 3096 |
|---|---|
| container_issue | 4 |
| container_start_page | 3081 |
| container_title | Brazilian journal of microbiology |
| container_volume | 55 |
| creator | da Silva, Alisson Teixeira Rosa, Danillo Sales Tavares, Marcio Rennan Santos Souza, Renata de Faria Silva Navarro, Daniela Maria do Amaral Ferraz de Aguiar, Júlio César Ribeiro de Oliveira Farias da Silva, Márcia Vanusa da Costa, Mateus Matiuzzi |
| description | Highlights
• The essential oils of the genus
Eugenia
show significant antibacterial activity.
• Essential oils combat the formation of
Staphylococcus aureus
biofilm responsible for bovine mastitis.
• The essential oils of the genus
Eugenia
act synergistically with oxacillin.
• The essential oil of
Eugenia stictopetala
was analyzed for the first time.
• The major components interact with penicillin-binding proteins, indicating a possible antibacterial mechanism.
Bovine mastitis, an inflammation of the mammary glands, is mainly caused by bacteria such as
Staphylococcus aureus
. While antibiotics are the primary treatment for this disease, their effectiveness is often diminished due to resistant strains and biofilm formation, creating the need for safer and more efficient therapies. Plant-based oil therapies, particularly those derived from the genus
Eugenia
, are gaining popularity due to their pharmacological potential and historical use. In this study, we evaluated the antibacterial, antibiofilm, and synergistic potential of essential oils (EOs) from four species of the genus
Eugenia
(
E. brejoensis
,
E. gracillima
,
E. pohliana
, and
E. stictopetala
) against
S. aureus
isolates from bovine mastitis. The EO of
E. stictopetala
was obtained by hydrodistillation, and its composition was analyzed using gas chromatography coupled with mass spectrometry. The experiment employed seven clinical isolates from mastitis and two control strains: ATCC 33591 (methicillin-resistant
S. aureus
- MRSA) and ATCC 25923 (methicillin-susceptible and biofilm producer). A broth microdilution assay was used to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the EOs and oxacillin. The EO of
E. stictopetala
contained (E)-caryophyllene (18.01%), β-pinene (8.84%), (E)-nerolidol (8.24%), and α-humulene (6.14%) as major compounds. In the MIC assay, all essential oils showed bactericidal and bacteriostatic effects, especially the species
E. brejoensis
and
E. pohliana
, which had MICs ranging from 64 to 256 µg/mL. Regarding the antibiofilm effect, all essential oils were capable of interfering with biofilm formation at subinhibitory concentrations of ½ and ¼ of the MIC. However, they did not significantly affect pre-established biofilms. Additionally, a synergistic interaction was detected between the EOs and oxacillin, with a reduction of 75–93.75% in the antimicrobial MIC. Molecular docking studies indicated that the phytochemicals β- |
| doi_str_mv | 10.1007/s42770-024-01489-6 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3097495416</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3097495416</sourcerecordid><originalsourceid>FETCH-LOGICAL-c228t-81039d6fefaea47d6adddca287ebebf139143c835010d7dbe3bf792f3045c7a93</originalsourceid><addsrcrecordid>eNp9UcuO1DAQtBCIfcAPcEA-LocsfiVOjmg1wEorcQDOVsfpzHqVxMHtLJpv4KfxMAtHTl3qriqpuhh7I8W1FMK-J6OsFZVQphLStF3VPGPnsrFtZYyonxdcS1u1ulVn7ILoQQhVC6NesjPdyU6oRpyzXzsiXHKAiccwEY8j3217XAJwWtdrfjUfUgaPgO843cefPCz8MeQUORRVDz5jOooLCGV_4LCHsFDmXzOs94cp-uj9Rhy2hGUEihNkJD6mOPM-PoYF-QyUQw70ir0YYSJ8_TQv2fePu283n6u7L59ubz7cVV6pNletFLobmhFHQDB2aGAYBg-qtdhjP8oSzmjf6lpIMdihR92PtlOjFqb2Fjp9ya5OvmuKPzak7OZAHqcJFowbOS06a7rayKZQ1YnqUyRKOLo1hRnSwUnhjiW4UwmulOD-lOCOordP_ls_4_BP8vfrhaBPBCqnZY_JPcQtLSXz_2x_Ay-HlkY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3097495416</pqid></control><display><type>article</type><title>Essential oils of Eugenia spp. (myrtaceae) show in vitro antibacterial activity against Staphylococcus aureus isolates from bovine mastitis</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><source>PubMed Central</source><creator>da Silva, Alisson Teixeira ; Rosa, Danillo Sales ; Tavares, Marcio Rennan Santos ; Souza, Renata de Faria Silva ; Navarro, Daniela Maria do Amaral Ferraz ; de Aguiar, Júlio César Ribeiro de Oliveira Farias ; da Silva, Márcia Vanusa ; da Costa, Mateus Matiuzzi</creator><creatorcontrib>da Silva, Alisson Teixeira ; Rosa, Danillo Sales ; Tavares, Marcio Rennan Santos ; Souza, Renata de Faria Silva ; Navarro, Daniela Maria do Amaral Ferraz ; de Aguiar, Júlio César Ribeiro de Oliveira Farias ; da Silva, Márcia Vanusa ; da Costa, Mateus Matiuzzi</creatorcontrib><description>Highlights
• The essential oils of the genus
Eugenia
show significant antibacterial activity.
• Essential oils combat the formation of
Staphylococcus aureus
biofilm responsible for bovine mastitis.
• The essential oils of the genus
Eugenia
act synergistically with oxacillin.
• The essential oil of
Eugenia stictopetala
was analyzed for the first time.
• The major components interact with penicillin-binding proteins, indicating a possible antibacterial mechanism.
Bovine mastitis, an inflammation of the mammary glands, is mainly caused by bacteria such as
Staphylococcus aureus
. While antibiotics are the primary treatment for this disease, their effectiveness is often diminished due to resistant strains and biofilm formation, creating the need for safer and more efficient therapies. Plant-based oil therapies, particularly those derived from the genus
Eugenia
, are gaining popularity due to their pharmacological potential and historical use. In this study, we evaluated the antibacterial, antibiofilm, and synergistic potential of essential oils (EOs) from four species of the genus
Eugenia
(
E. brejoensis
,
E. gracillima
,
E. pohliana
, and
E. stictopetala
) against
S. aureus
isolates from bovine mastitis. The EO of
E. stictopetala
was obtained by hydrodistillation, and its composition was analyzed using gas chromatography coupled with mass spectrometry. The experiment employed seven clinical isolates from mastitis and two control strains: ATCC 33591 (methicillin-resistant
S. aureus
- MRSA) and ATCC 25923 (methicillin-susceptible and biofilm producer). A broth microdilution assay was used to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the EOs and oxacillin. The EO of
E. stictopetala
contained (E)-caryophyllene (18.01%), β-pinene (8.84%), (E)-nerolidol (8.24%), and α-humulene (6.14%) as major compounds. In the MIC assay, all essential oils showed bactericidal and bacteriostatic effects, especially the species
E. brejoensis
and
E. pohliana
, which had MICs ranging from 64 to 256 µg/mL. Regarding the antibiofilm effect, all essential oils were capable of interfering with biofilm formation at subinhibitory concentrations of ½ and ¼ of the MIC. However, they did not significantly affect pre-established biofilms. Additionally, a synergistic interaction was detected between the EOs and oxacillin, with a reduction of 75–93.75% in the antimicrobial MIC. Molecular docking studies indicated that the phytochemicals β-(E)-caryophyllene, (E)-nerolidol, Δ-elemene, and α-cadinol present in the EOs formed more stable complexes with penicillin-binding proteins, indicating a possible mechanism of antibacterial action. Therefore, these results show that the essential oils of
Eugenia
spp. are promising sources for the development of new therapeutic methods, opening new perspectives for a more effective treatment of bovine mastitis.</description><identifier>ISSN: 1517-8382</identifier><identifier>ISSN: 1678-4405</identifier><identifier>EISSN: 1678-4405</identifier><identifier>DOI: 10.1007/s42770-024-01489-6</identifier><identifier>PMID: 39190260</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Animals ; Anti-Bacterial Agents - chemistry ; Anti-Bacterial Agents - pharmacology ; Biofilms - drug effects ; Biomedical and Life Sciences ; Biotechnology and Industrial Microbiology - Research Paper ; Cattle ; Eugenia - chemistry ; Female ; Food Microbiology ; Life Sciences ; Mastitis, Bovine - drug therapy ; Mastitis, Bovine - microbiology ; Medical Microbiology ; Microbial Ecology ; Microbial Genetics and Genomics ; Microbial Sensitivity Tests ; Microbiology ; Mycology ; Oils, Volatile - chemistry ; Oils, Volatile - pharmacology ; Plant Oils - chemistry ; Plant Oils - pharmacology ; Staphylococcal Infections - drug therapy ; Staphylococcal Infections - microbiology ; Staphylococcus aureus - drug effects</subject><ispartof>Brazilian journal of microbiology, 2024-12, Vol.55 (4), p.3081-3096</ispartof><rights>The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia 2024 Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c228t-81039d6fefaea47d6adddca287ebebf139143c835010d7dbe3bf792f3045c7a93</cites><orcidid>0000-0003-4586-3291 ; 0000-0002-2221-5059 ; 0000-0002-0447-5041 ; 0000-0003-0158-7221 ; 0000-0001-5621-4831 ; 0000-0002-9072-0727 ; 0000-0003-0481-248X ; 0000-0002-9884-2112</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s42770-024-01489-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s42770-024-01489-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51298</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39190260$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>da Silva, Alisson Teixeira</creatorcontrib><creatorcontrib>Rosa, Danillo Sales</creatorcontrib><creatorcontrib>Tavares, Marcio Rennan Santos</creatorcontrib><creatorcontrib>Souza, Renata de Faria Silva</creatorcontrib><creatorcontrib>Navarro, Daniela Maria do Amaral Ferraz</creatorcontrib><creatorcontrib>de Aguiar, Júlio César Ribeiro de Oliveira Farias</creatorcontrib><creatorcontrib>da Silva, Márcia Vanusa</creatorcontrib><creatorcontrib>da Costa, Mateus Matiuzzi</creatorcontrib><title>Essential oils of Eugenia spp. (myrtaceae) show in vitro antibacterial activity against Staphylococcus aureus isolates from bovine mastitis</title><title>Brazilian journal of microbiology</title><addtitle>Braz J Microbiol</addtitle><addtitle>Braz J Microbiol</addtitle><description>Highlights
• The essential oils of the genus
Eugenia
show significant antibacterial activity.
• Essential oils combat the formation of
Staphylococcus aureus
biofilm responsible for bovine mastitis.
• The essential oils of the genus
Eugenia
act synergistically with oxacillin.
• The essential oil of
Eugenia stictopetala
was analyzed for the first time.
• The major components interact with penicillin-binding proteins, indicating a possible antibacterial mechanism.
Bovine mastitis, an inflammation of the mammary glands, is mainly caused by bacteria such as
Staphylococcus aureus
. While antibiotics are the primary treatment for this disease, their effectiveness is often diminished due to resistant strains and biofilm formation, creating the need for safer and more efficient therapies. Plant-based oil therapies, particularly those derived from the genus
Eugenia
, are gaining popularity due to their pharmacological potential and historical use. In this study, we evaluated the antibacterial, antibiofilm, and synergistic potential of essential oils (EOs) from four species of the genus
Eugenia
(
E. brejoensis
,
E. gracillima
,
E. pohliana
, and
E. stictopetala
) against
S. aureus
isolates from bovine mastitis. The EO of
E. stictopetala
was obtained by hydrodistillation, and its composition was analyzed using gas chromatography coupled with mass spectrometry. The experiment employed seven clinical isolates from mastitis and two control strains: ATCC 33591 (methicillin-resistant
S. aureus
- MRSA) and ATCC 25923 (methicillin-susceptible and biofilm producer). A broth microdilution assay was used to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the EOs and oxacillin. The EO of
E. stictopetala
contained (E)-caryophyllene (18.01%), β-pinene (8.84%), (E)-nerolidol (8.24%), and α-humulene (6.14%) as major compounds. In the MIC assay, all essential oils showed bactericidal and bacteriostatic effects, especially the species
E. brejoensis
and
E. pohliana
, which had MICs ranging from 64 to 256 µg/mL. Regarding the antibiofilm effect, all essential oils were capable of interfering with biofilm formation at subinhibitory concentrations of ½ and ¼ of the MIC. However, they did not significantly affect pre-established biofilms. Additionally, a synergistic interaction was detected between the EOs and oxacillin, with a reduction of 75–93.75% in the antimicrobial MIC. Molecular docking studies indicated that the phytochemicals β-(E)-caryophyllene, (E)-nerolidol, Δ-elemene, and α-cadinol present in the EOs formed more stable complexes with penicillin-binding proteins, indicating a possible mechanism of antibacterial action. Therefore, these results show that the essential oils of
Eugenia
spp. are promising sources for the development of new therapeutic methods, opening new perspectives for a more effective treatment of bovine mastitis.</description><subject>Animals</subject><subject>Anti-Bacterial Agents - chemistry</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Biofilms - drug effects</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology and Industrial Microbiology - Research Paper</subject><subject>Cattle</subject><subject>Eugenia - chemistry</subject><subject>Female</subject><subject>Food Microbiology</subject><subject>Life Sciences</subject><subject>Mastitis, Bovine - drug therapy</subject><subject>Mastitis, Bovine - microbiology</subject><subject>Medical Microbiology</subject><subject>Microbial Ecology</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbial Sensitivity Tests</subject><subject>Microbiology</subject><subject>Mycology</subject><subject>Oils, Volatile - chemistry</subject><subject>Oils, Volatile - pharmacology</subject><subject>Plant Oils - chemistry</subject><subject>Plant Oils - pharmacology</subject><subject>Staphylococcal Infections - drug therapy</subject><subject>Staphylococcal Infections - microbiology</subject><subject>Staphylococcus aureus - drug effects</subject><issn>1517-8382</issn><issn>1678-4405</issn><issn>1678-4405</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UcuO1DAQtBCIfcAPcEA-LocsfiVOjmg1wEorcQDOVsfpzHqVxMHtLJpv4KfxMAtHTl3qriqpuhh7I8W1FMK-J6OsFZVQphLStF3VPGPnsrFtZYyonxdcS1u1ulVn7ILoQQhVC6NesjPdyU6oRpyzXzsiXHKAiccwEY8j3217XAJwWtdrfjUfUgaPgO843cefPCz8MeQUORRVDz5jOooLCGV_4LCHsFDmXzOs94cp-uj9Rhy2hGUEihNkJD6mOPM-PoYF-QyUQw70ir0YYSJ8_TQv2fePu283n6u7L59ubz7cVV6pNletFLobmhFHQDB2aGAYBg-qtdhjP8oSzmjf6lpIMdihR92PtlOjFqb2Fjp9ya5OvmuKPzak7OZAHqcJFowbOS06a7rayKZQ1YnqUyRKOLo1hRnSwUnhjiW4UwmulOD-lOCOordP_ls_4_BP8vfrhaBPBCqnZY_JPcQtLSXz_2x_Ay-HlkY</recordid><startdate>20241201</startdate><enddate>20241201</enddate><creator>da Silva, Alisson Teixeira</creator><creator>Rosa, Danillo Sales</creator><creator>Tavares, Marcio Rennan Santos</creator><creator>Souza, Renata de Faria Silva</creator><creator>Navarro, Daniela Maria do Amaral Ferraz</creator><creator>de Aguiar, Júlio César Ribeiro de Oliveira Farias</creator><creator>da Silva, Márcia Vanusa</creator><creator>da Costa, Mateus Matiuzzi</creator><general>Springer International Publishing</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-4586-3291</orcidid><orcidid>https://orcid.org/0000-0002-2221-5059</orcidid><orcidid>https://orcid.org/0000-0002-0447-5041</orcidid><orcidid>https://orcid.org/0000-0003-0158-7221</orcidid><orcidid>https://orcid.org/0000-0001-5621-4831</orcidid><orcidid>https://orcid.org/0000-0002-9072-0727</orcidid><orcidid>https://orcid.org/0000-0003-0481-248X</orcidid><orcidid>https://orcid.org/0000-0002-9884-2112</orcidid></search><sort><creationdate>20241201</creationdate><title>Essential oils of Eugenia spp. (myrtaceae) show in vitro antibacterial activity against Staphylococcus aureus isolates from bovine mastitis</title><author>da Silva, Alisson Teixeira ; Rosa, Danillo Sales ; Tavares, Marcio Rennan Santos ; Souza, Renata de Faria Silva ; Navarro, Daniela Maria do Amaral Ferraz ; de Aguiar, Júlio César Ribeiro de Oliveira Farias ; da Silva, Márcia Vanusa ; da Costa, Mateus Matiuzzi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c228t-81039d6fefaea47d6adddca287ebebf139143c835010d7dbe3bf792f3045c7a93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Animals</topic><topic>Anti-Bacterial Agents - chemistry</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Biofilms - drug effects</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology and Industrial Microbiology - Research Paper</topic><topic>Cattle</topic><topic>Eugenia - chemistry</topic><topic>Female</topic><topic>Food Microbiology</topic><topic>Life Sciences</topic><topic>Mastitis, Bovine - drug therapy</topic><topic>Mastitis, Bovine - microbiology</topic><topic>Medical Microbiology</topic><topic>Microbial Ecology</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbial Sensitivity Tests</topic><topic>Microbiology</topic><topic>Mycology</topic><topic>Oils, Volatile - chemistry</topic><topic>Oils, Volatile - pharmacology</topic><topic>Plant Oils - chemistry</topic><topic>Plant Oils - pharmacology</topic><topic>Staphylococcal Infections - drug therapy</topic><topic>Staphylococcal Infections - microbiology</topic><topic>Staphylococcus aureus - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>da Silva, Alisson Teixeira</creatorcontrib><creatorcontrib>Rosa, Danillo Sales</creatorcontrib><creatorcontrib>Tavares, Marcio Rennan Santos</creatorcontrib><creatorcontrib>Souza, Renata de Faria Silva</creatorcontrib><creatorcontrib>Navarro, Daniela Maria do Amaral Ferraz</creatorcontrib><creatorcontrib>de Aguiar, Júlio César Ribeiro de Oliveira Farias</creatorcontrib><creatorcontrib>da Silva, Márcia Vanusa</creatorcontrib><creatorcontrib>da Costa, Mateus Matiuzzi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Brazilian journal of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>da Silva, Alisson Teixeira</au><au>Rosa, Danillo Sales</au><au>Tavares, Marcio Rennan Santos</au><au>Souza, Renata de Faria Silva</au><au>Navarro, Daniela Maria do Amaral Ferraz</au><au>de Aguiar, Júlio César Ribeiro de Oliveira Farias</au><au>da Silva, Márcia Vanusa</au><au>da Costa, Mateus Matiuzzi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Essential oils of Eugenia spp. (myrtaceae) show in vitro antibacterial activity against Staphylococcus aureus isolates from bovine mastitis</atitle><jtitle>Brazilian journal of microbiology</jtitle><stitle>Braz J Microbiol</stitle><addtitle>Braz J Microbiol</addtitle><date>2024-12-01</date><risdate>2024</risdate><volume>55</volume><issue>4</issue><spage>3081</spage><epage>3096</epage><pages>3081-3096</pages><issn>1517-8382</issn><issn>1678-4405</issn><eissn>1678-4405</eissn><abstract>Highlights
• The essential oils of the genus
Eugenia
show significant antibacterial activity.
• Essential oils combat the formation of
Staphylococcus aureus
biofilm responsible for bovine mastitis.
• The essential oils of the genus
Eugenia
act synergistically with oxacillin.
• The essential oil of
Eugenia stictopetala
was analyzed for the first time.
• The major components interact with penicillin-binding proteins, indicating a possible antibacterial mechanism.
Bovine mastitis, an inflammation of the mammary glands, is mainly caused by bacteria such as
Staphylococcus aureus
. While antibiotics are the primary treatment for this disease, their effectiveness is often diminished due to resistant strains and biofilm formation, creating the need for safer and more efficient therapies. Plant-based oil therapies, particularly those derived from the genus
Eugenia
, are gaining popularity due to their pharmacological potential and historical use. In this study, we evaluated the antibacterial, antibiofilm, and synergistic potential of essential oils (EOs) from four species of the genus
Eugenia
(
E. brejoensis
,
E. gracillima
,
E. pohliana
, and
E. stictopetala
) against
S. aureus
isolates from bovine mastitis. The EO of
E. stictopetala
was obtained by hydrodistillation, and its composition was analyzed using gas chromatography coupled with mass spectrometry. The experiment employed seven clinical isolates from mastitis and two control strains: ATCC 33591 (methicillin-resistant
S. aureus
- MRSA) and ATCC 25923 (methicillin-susceptible and biofilm producer). A broth microdilution assay was used to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the EOs and oxacillin. The EO of
E. stictopetala
contained (E)-caryophyllene (18.01%), β-pinene (8.84%), (E)-nerolidol (8.24%), and α-humulene (6.14%) as major compounds. In the MIC assay, all essential oils showed bactericidal and bacteriostatic effects, especially the species
E. brejoensis
and
E. pohliana
, which had MICs ranging from 64 to 256 µg/mL. Regarding the antibiofilm effect, all essential oils were capable of interfering with biofilm formation at subinhibitory concentrations of ½ and ¼ of the MIC. However, they did not significantly affect pre-established biofilms. Additionally, a synergistic interaction was detected between the EOs and oxacillin, with a reduction of 75–93.75% in the antimicrobial MIC. Molecular docking studies indicated that the phytochemicals β-(E)-caryophyllene, (E)-nerolidol, Δ-elemene, and α-cadinol present in the EOs formed more stable complexes with penicillin-binding proteins, indicating a possible mechanism of antibacterial action. Therefore, these results show that the essential oils of
Eugenia
spp. are promising sources for the development of new therapeutic methods, opening new perspectives for a more effective treatment of bovine mastitis.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>39190260</pmid><doi>10.1007/s42770-024-01489-6</doi><tpages>16</tpages><orcidid>https://orcid.org/0000-0003-4586-3291</orcidid><orcidid>https://orcid.org/0000-0002-2221-5059</orcidid><orcidid>https://orcid.org/0000-0002-0447-5041</orcidid><orcidid>https://orcid.org/0000-0003-0158-7221</orcidid><orcidid>https://orcid.org/0000-0001-5621-4831</orcidid><orcidid>https://orcid.org/0000-0002-9072-0727</orcidid><orcidid>https://orcid.org/0000-0003-0481-248X</orcidid><orcidid>https://orcid.org/0000-0002-9884-2112</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1517-8382 |
| ispartof | Brazilian journal of microbiology, 2024-12, Vol.55 (4), p.3081-3096 |
| issn | 1517-8382 1678-4405 1678-4405 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_3097495416 |
| source | MEDLINE; Springer Nature - Complete Springer Journals; PubMed Central |
| subjects | Animals Anti-Bacterial Agents - chemistry Anti-Bacterial Agents - pharmacology Biofilms - drug effects Biomedical and Life Sciences Biotechnology and Industrial Microbiology - Research Paper Cattle Eugenia - chemistry Female Food Microbiology Life Sciences Mastitis, Bovine - drug therapy Mastitis, Bovine - microbiology Medical Microbiology Microbial Ecology Microbial Genetics and Genomics Microbial Sensitivity Tests Microbiology Mycology Oils, Volatile - chemistry Oils, Volatile - pharmacology Plant Oils - chemistry Plant Oils - pharmacology Staphylococcal Infections - drug therapy Staphylococcal Infections - microbiology Staphylococcus aureus - drug effects |
| title | Essential oils of Eugenia spp. (myrtaceae) show in vitro antibacterial activity against Staphylococcus aureus isolates from bovine mastitis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-23T10%3A49%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Essential%20oils%20of%20Eugenia%20spp.%20(myrtaceae)%20show%20in%20vitro%20antibacterial%20activity%20against%20Staphylococcus%20aureus%20isolates%20from%20bovine%20mastitis&rft.jtitle=Brazilian%20journal%20of%20microbiology&rft.au=da%20Silva,%20Alisson%20Teixeira&rft.date=2024-12-01&rft.volume=55&rft.issue=4&rft.spage=3081&rft.epage=3096&rft.pages=3081-3096&rft.issn=1517-8382&rft.eissn=1678-4405&rft_id=info:doi/10.1007/s42770-024-01489-6&rft_dat=%3Cproquest_cross%3E3097495416%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3097495416&rft_id=info:pmid/39190260&rfr_iscdi=true |