Duration of PCR positivity by type of respiratory virus among children using a multiplex PCR test
Prolonged positive polymerase chain reaction (PCR) results, irrespective of the transmission risk, can lead to prolonged restrictions on daily activities and infection precaution interventions. Studies evaluating the duration of PCR positivity for multiple pathogens in a single patient cohort are sc...
Gespeichert in:
| Veröffentlicht in: | Journal of medical virology 2024-08, Vol.96 (8), p.e29890-n/a |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | n/a |
|---|---|
| container_issue | 8 |
| container_start_page | e29890 |
| container_title | Journal of medical virology |
| container_volume | 96 |
| creator | Kitano, Taito Kitagawa, Daisuke Murata, Masayuki Onishi, Mai Mori, Takahiro Hachisuka, Soshi Okubo, Tenshin Yamamoto, Naohiro Nishikawa, Hiroki Onaka, Masayuki Suzuki, Rika Sekine, Madoka Suzuki, Soma Nakamura, Fumihiko Yoshida, Sayaka |
| description | Prolonged positive polymerase chain reaction (PCR) results, irrespective of the transmission risk, can lead to prolonged restrictions on daily activities and infection precaution interventions. Studies evaluating the duration of PCR positivity for multiple pathogens in a single patient cohort are scarce. This study aimed to evaluate and compare the durations of PCR positivity for multiple respiratory viruses among children and adolescents. This retrospective study was conducted between April 2018 and March 2024 using a multiplex PCR respiratory panel for symptomatic children and adolescents who had at least two tests within 90 days of study period, with the first PCR test positive. The rate and likelihood of persistent PCR positivity were evaluated for multiple respiratory viruses. For 1325 positive results, repeat tests were conducted within 90 days. The persistent PCR positivity rate at repeat testing decreased over time (60.6%, Days 1–15 and 21.7%, Days 76–90, after the first test). In multivariate logistic regression analysis, an increased likelihood of persistent PCR positivity was observed for rhinovirus/enterovirus and adenovirus, whereas decreased likelihood of persistent positivity was seen in influenza and seasonal coronaviruses, compared with parainfluenza viruses. Persistent PCR positivity is common for multiple respiratory viruses in symptomatic children. |
| doi_str_mv | 10.1002/jmv.29890 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3097494381</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3097618941</sourcerecordid><originalsourceid>FETCH-LOGICAL-c2430-ae25f7b2152828312a81e3ac5cd1531f41bdca0afd254835a537466ba7f0c0aa3</originalsourceid><addsrcrecordid>eNp10MtKxDAUBuAgijOOLnwBCbjRRZ2TpJdkKeOdEUXUbUnbVDO0TU3a0b69nenoQnAVDvn4z-FH6JDAGQGg00W5PKOCC9hCYwIi9AREZBuNgfihF4YkGKE95xYAwAWlu2jEBOEcQjFG8qK1stGmwibHj7MnXBunG73UTYeTDjddrVY_Vrla99DYDi-1bR2WpanecPqui8yqCrdO96PEZVs0ui7U1zqsUa7ZRzu5LJw62LwT9HJ1-Ty78eYP17ez87mXUp-BJxUN8iihJKCcckao5EQxmQZpRgJGcp8kWSpB5hkNfM4CGbDID8NERjmkICWboJMht7bmo-0Xx6V2qSoKWSnTupiBiHzhM056evyHLkxrq_66tQoJF_5KnQ4qtcY5q_K4trqUtosJxKve4773eN17b482iW1SquxX_hTdg-kAPnWhuv-T4rv71yHyG1hpjDw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3097618941</pqid></control><display><type>article</type><title>Duration of PCR positivity by type of respiratory virus among children using a multiplex PCR test</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><creator>Kitano, Taito ; Kitagawa, Daisuke ; Murata, Masayuki ; Onishi, Mai ; Mori, Takahiro ; Hachisuka, Soshi ; Okubo, Tenshin ; Yamamoto, Naohiro ; Nishikawa, Hiroki ; Onaka, Masayuki ; Suzuki, Rika ; Sekine, Madoka ; Suzuki, Soma ; Nakamura, Fumihiko ; Yoshida, Sayaka</creator><creatorcontrib>Kitano, Taito ; Kitagawa, Daisuke ; Murata, Masayuki ; Onishi, Mai ; Mori, Takahiro ; Hachisuka, Soshi ; Okubo, Tenshin ; Yamamoto, Naohiro ; Nishikawa, Hiroki ; Onaka, Masayuki ; Suzuki, Rika ; Sekine, Madoka ; Suzuki, Soma ; Nakamura, Fumihiko ; Yoshida, Sayaka</creatorcontrib><description>Prolonged positive polymerase chain reaction (PCR) results, irrespective of the transmission risk, can lead to prolonged restrictions on daily activities and infection precaution interventions. Studies evaluating the duration of PCR positivity for multiple pathogens in a single patient cohort are scarce. This study aimed to evaluate and compare the durations of PCR positivity for multiple respiratory viruses among children and adolescents. This retrospective study was conducted between April 2018 and March 2024 using a multiplex PCR respiratory panel for symptomatic children and adolescents who had at least two tests within 90 days of study period, with the first PCR test positive. The rate and likelihood of persistent PCR positivity were evaluated for multiple respiratory viruses. For 1325 positive results, repeat tests were conducted within 90 days. The persistent PCR positivity rate at repeat testing decreased over time (60.6%, Days 1–15 and 21.7%, Days 76–90, after the first test). In multivariate logistic regression analysis, an increased likelihood of persistent PCR positivity was observed for rhinovirus/enterovirus and adenovirus, whereas decreased likelihood of persistent positivity was seen in influenza and seasonal coronaviruses, compared with parainfluenza viruses. Persistent PCR positivity is common for multiple respiratory viruses in symptomatic children.</description><identifier>ISSN: 0146-6615</identifier><identifier>ISSN: 1096-9071</identifier><identifier>EISSN: 1096-9071</identifier><identifier>DOI: 10.1002/jmv.29890</identifier><identifier>PMID: 39188069</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>adenovirus ; Adolescent ; Adolescents ; Child ; Child, Preschool ; Children ; Coronaviruses ; COVID‐19 ; Enterovirus - classification ; Enterovirus - genetics ; Enterovirus - isolation & purification ; Enteroviruses ; Female ; Humans ; Infant ; influenza ; Male ; multiplex polymerase chain reaction ; Multiplex Polymerase Chain Reaction - methods ; Multiplexing ; Parainfluenza ; Polymerase chain reaction ; Regression analysis ; respiratory syncytial virus infections ; respiratory tract infections ; Respiratory Tract Infections - diagnosis ; Respiratory Tract Infections - virology ; Retrospective Studies ; Rhinovirus ; Rhinovirus - genetics ; Rhinovirus - isolation & purification ; SARS‐CoV‐2 ; Time Factors ; Virus Diseases - diagnosis ; Virus Diseases - virology ; Viruses ; Viruses - classification ; Viruses - genetics ; Viruses - isolation & purification</subject><ispartof>Journal of medical virology, 2024-08, Vol.96 (8), p.e29890-n/a</ispartof><rights>2024 Wiley Periodicals LLC.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c2430-ae25f7b2152828312a81e3ac5cd1531f41bdca0afd254835a537466ba7f0c0aa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjmv.29890$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjmv.29890$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39188069$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kitano, Taito</creatorcontrib><creatorcontrib>Kitagawa, Daisuke</creatorcontrib><creatorcontrib>Murata, Masayuki</creatorcontrib><creatorcontrib>Onishi, Mai</creatorcontrib><creatorcontrib>Mori, Takahiro</creatorcontrib><creatorcontrib>Hachisuka, Soshi</creatorcontrib><creatorcontrib>Okubo, Tenshin</creatorcontrib><creatorcontrib>Yamamoto, Naohiro</creatorcontrib><creatorcontrib>Nishikawa, Hiroki</creatorcontrib><creatorcontrib>Onaka, Masayuki</creatorcontrib><creatorcontrib>Suzuki, Rika</creatorcontrib><creatorcontrib>Sekine, Madoka</creatorcontrib><creatorcontrib>Suzuki, Soma</creatorcontrib><creatorcontrib>Nakamura, Fumihiko</creatorcontrib><creatorcontrib>Yoshida, Sayaka</creatorcontrib><title>Duration of PCR positivity by type of respiratory virus among children using a multiplex PCR test</title><title>Journal of medical virology</title><addtitle>J Med Virol</addtitle><description>Prolonged positive polymerase chain reaction (PCR) results, irrespective of the transmission risk, can lead to prolonged restrictions on daily activities and infection precaution interventions. Studies evaluating the duration of PCR positivity for multiple pathogens in a single patient cohort are scarce. This study aimed to evaluate and compare the durations of PCR positivity for multiple respiratory viruses among children and adolescents. This retrospective study was conducted between April 2018 and March 2024 using a multiplex PCR respiratory panel for symptomatic children and adolescents who had at least two tests within 90 days of study period, with the first PCR test positive. The rate and likelihood of persistent PCR positivity were evaluated for multiple respiratory viruses. For 1325 positive results, repeat tests were conducted within 90 days. The persistent PCR positivity rate at repeat testing decreased over time (60.6%, Days 1–15 and 21.7%, Days 76–90, after the first test). In multivariate logistic regression analysis, an increased likelihood of persistent PCR positivity was observed for rhinovirus/enterovirus and adenovirus, whereas decreased likelihood of persistent positivity was seen in influenza and seasonal coronaviruses, compared with parainfluenza viruses. Persistent PCR positivity is common for multiple respiratory viruses in symptomatic children.</description><subject>adenovirus</subject><subject>Adolescent</subject><subject>Adolescents</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Children</subject><subject>Coronaviruses</subject><subject>COVID‐19</subject><subject>Enterovirus - classification</subject><subject>Enterovirus - genetics</subject><subject>Enterovirus - isolation & purification</subject><subject>Enteroviruses</subject><subject>Female</subject><subject>Humans</subject><subject>Infant</subject><subject>influenza</subject><subject>Male</subject><subject>multiplex polymerase chain reaction</subject><subject>Multiplex Polymerase Chain Reaction - methods</subject><subject>Multiplexing</subject><subject>Parainfluenza</subject><subject>Polymerase chain reaction</subject><subject>Regression analysis</subject><subject>respiratory syncytial virus infections</subject><subject>respiratory tract infections</subject><subject>Respiratory Tract Infections - diagnosis</subject><subject>Respiratory Tract Infections - virology</subject><subject>Retrospective Studies</subject><subject>Rhinovirus</subject><subject>Rhinovirus - genetics</subject><subject>Rhinovirus - isolation & purification</subject><subject>SARS‐CoV‐2</subject><subject>Time Factors</subject><subject>Virus Diseases - diagnosis</subject><subject>Virus Diseases - virology</subject><subject>Viruses</subject><subject>Viruses - classification</subject><subject>Viruses - genetics</subject><subject>Viruses - isolation & purification</subject><issn>0146-6615</issn><issn>1096-9071</issn><issn>1096-9071</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10MtKxDAUBuAgijOOLnwBCbjRRZ2TpJdkKeOdEUXUbUnbVDO0TU3a0b69nenoQnAVDvn4z-FH6JDAGQGg00W5PKOCC9hCYwIi9AREZBuNgfihF4YkGKE95xYAwAWlu2jEBOEcQjFG8qK1stGmwibHj7MnXBunG73UTYeTDjddrVY_Vrla99DYDi-1bR2WpanecPqui8yqCrdO96PEZVs0ui7U1zqsUa7ZRzu5LJw62LwT9HJ1-Ty78eYP17ez87mXUp-BJxUN8iihJKCcckao5EQxmQZpRgJGcp8kWSpB5hkNfM4CGbDID8NERjmkICWboJMht7bmo-0Xx6V2qSoKWSnTupiBiHzhM056evyHLkxrq_66tQoJF_5KnQ4qtcY5q_K4trqUtosJxKve4773eN17b482iW1SquxX_hTdg-kAPnWhuv-T4rv71yHyG1hpjDw</recordid><startdate>202408</startdate><enddate>202408</enddate><creator>Kitano, Taito</creator><creator>Kitagawa, Daisuke</creator><creator>Murata, Masayuki</creator><creator>Onishi, Mai</creator><creator>Mori, Takahiro</creator><creator>Hachisuka, Soshi</creator><creator>Okubo, Tenshin</creator><creator>Yamamoto, Naohiro</creator><creator>Nishikawa, Hiroki</creator><creator>Onaka, Masayuki</creator><creator>Suzuki, Rika</creator><creator>Sekine, Madoka</creator><creator>Suzuki, Soma</creator><creator>Nakamura, Fumihiko</creator><creator>Yoshida, Sayaka</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>202408</creationdate><title>Duration of PCR positivity by type of respiratory virus among children using a multiplex PCR test</title><author>Kitano, Taito ; Kitagawa, Daisuke ; Murata, Masayuki ; Onishi, Mai ; Mori, Takahiro ; Hachisuka, Soshi ; Okubo, Tenshin ; Yamamoto, Naohiro ; Nishikawa, Hiroki ; Onaka, Masayuki ; Suzuki, Rika ; Sekine, Madoka ; Suzuki, Soma ; Nakamura, Fumihiko ; Yoshida, Sayaka</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2430-ae25f7b2152828312a81e3ac5cd1531f41bdca0afd254835a537466ba7f0c0aa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>adenovirus</topic><topic>Adolescent</topic><topic>Adolescents</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Children</topic><topic>Coronaviruses</topic><topic>COVID‐19</topic><topic>Enterovirus - classification</topic><topic>Enterovirus - genetics</topic><topic>Enterovirus - isolation & purification</topic><topic>Enteroviruses</topic><topic>Female</topic><topic>Humans</topic><topic>Infant</topic><topic>influenza</topic><topic>Male</topic><topic>multiplex polymerase chain reaction</topic><topic>Multiplex Polymerase Chain Reaction - methods</topic><topic>Multiplexing</topic><topic>Parainfluenza</topic><topic>Polymerase chain reaction</topic><topic>Regression analysis</topic><topic>respiratory syncytial virus infections</topic><topic>respiratory tract infections</topic><topic>Respiratory Tract Infections - diagnosis</topic><topic>Respiratory Tract Infections - virology</topic><topic>Retrospective Studies</topic><topic>Rhinovirus</topic><topic>Rhinovirus - genetics</topic><topic>Rhinovirus - isolation & purification</topic><topic>SARS‐CoV‐2</topic><topic>Time Factors</topic><topic>Virus Diseases - diagnosis</topic><topic>Virus Diseases - virology</topic><topic>Viruses</topic><topic>Viruses - classification</topic><topic>Viruses - genetics</topic><topic>Viruses - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kitano, Taito</creatorcontrib><creatorcontrib>Kitagawa, Daisuke</creatorcontrib><creatorcontrib>Murata, Masayuki</creatorcontrib><creatorcontrib>Onishi, Mai</creatorcontrib><creatorcontrib>Mori, Takahiro</creatorcontrib><creatorcontrib>Hachisuka, Soshi</creatorcontrib><creatorcontrib>Okubo, Tenshin</creatorcontrib><creatorcontrib>Yamamoto, Naohiro</creatorcontrib><creatorcontrib>Nishikawa, Hiroki</creatorcontrib><creatorcontrib>Onaka, Masayuki</creatorcontrib><creatorcontrib>Suzuki, Rika</creatorcontrib><creatorcontrib>Sekine, Madoka</creatorcontrib><creatorcontrib>Suzuki, Soma</creatorcontrib><creatorcontrib>Nakamura, Fumihiko</creatorcontrib><creatorcontrib>Yoshida, Sayaka</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of medical virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kitano, Taito</au><au>Kitagawa, Daisuke</au><au>Murata, Masayuki</au><au>Onishi, Mai</au><au>Mori, Takahiro</au><au>Hachisuka, Soshi</au><au>Okubo, Tenshin</au><au>Yamamoto, Naohiro</au><au>Nishikawa, Hiroki</au><au>Onaka, Masayuki</au><au>Suzuki, Rika</au><au>Sekine, Madoka</au><au>Suzuki, Soma</au><au>Nakamura, Fumihiko</au><au>Yoshida, Sayaka</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Duration of PCR positivity by type of respiratory virus among children using a multiplex PCR test</atitle><jtitle>Journal of medical virology</jtitle><addtitle>J Med Virol</addtitle><date>2024-08</date><risdate>2024</risdate><volume>96</volume><issue>8</issue><spage>e29890</spage><epage>n/a</epage><pages>e29890-n/a</pages><issn>0146-6615</issn><issn>1096-9071</issn><eissn>1096-9071</eissn><abstract>Prolonged positive polymerase chain reaction (PCR) results, irrespective of the transmission risk, can lead to prolonged restrictions on daily activities and infection precaution interventions. Studies evaluating the duration of PCR positivity for multiple pathogens in a single patient cohort are scarce. This study aimed to evaluate and compare the durations of PCR positivity for multiple respiratory viruses among children and adolescents. This retrospective study was conducted between April 2018 and March 2024 using a multiplex PCR respiratory panel for symptomatic children and adolescents who had at least two tests within 90 days of study period, with the first PCR test positive. The rate and likelihood of persistent PCR positivity were evaluated for multiple respiratory viruses. For 1325 positive results, repeat tests were conducted within 90 days. The persistent PCR positivity rate at repeat testing decreased over time (60.6%, Days 1–15 and 21.7%, Days 76–90, after the first test). In multivariate logistic regression analysis, an increased likelihood of persistent PCR positivity was observed for rhinovirus/enterovirus and adenovirus, whereas decreased likelihood of persistent positivity was seen in influenza and seasonal coronaviruses, compared with parainfluenza viruses. Persistent PCR positivity is common for multiple respiratory viruses in symptomatic children.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>39188069</pmid><doi>10.1002/jmv.29890</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0146-6615 |
| ispartof | Journal of medical virology, 2024-08, Vol.96 (8), p.e29890-n/a |
| issn | 0146-6615 1096-9071 1096-9071 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_3097494381 |
| source | MEDLINE; Access via Wiley Online Library |
| subjects | adenovirus Adolescent Adolescents Child Child, Preschool Children Coronaviruses COVID‐19 Enterovirus - classification Enterovirus - genetics Enterovirus - isolation & purification Enteroviruses Female Humans Infant influenza Male multiplex polymerase chain reaction Multiplex Polymerase Chain Reaction - methods Multiplexing Parainfluenza Polymerase chain reaction Regression analysis respiratory syncytial virus infections respiratory tract infections Respiratory Tract Infections - diagnosis Respiratory Tract Infections - virology Retrospective Studies Rhinovirus Rhinovirus - genetics Rhinovirus - isolation & purification SARS‐CoV‐2 Time Factors Virus Diseases - diagnosis Virus Diseases - virology Viruses Viruses - classification Viruses - genetics Viruses - isolation & purification |
| title | Duration of PCR positivity by type of respiratory virus among children using a multiplex PCR test |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-20T00%3A48%3A15IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Duration%20of%20PCR%20positivity%20by%20type%20of%20respiratory%20virus%20among%20children%20using%20a%20multiplex%20PCR%20test&rft.jtitle=Journal%20of%20medical%20virology&rft.au=Kitano,%20Taito&rft.date=2024-08&rft.volume=96&rft.issue=8&rft.spage=e29890&rft.epage=n/a&rft.pages=e29890-n/a&rft.issn=0146-6615&rft.eissn=1096-9071&rft_id=info:doi/10.1002/jmv.29890&rft_dat=%3Cproquest_cross%3E3097618941%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3097618941&rft_id=info:pmid/39188069&rfr_iscdi=true |