IP3R1 is required for meiotic progression and embryonic development by regulating mitochondrial calcium and oxidative damage
Calcium ions (Ca2+) regulate cell proliferation and differentiation and participate in various physiological activities of cells. The calcium transfer protein inositol 1,4,5-triphosphate receptor (IP3R), located between the endoplasmic reticulum (ER) and mitochondria, plays an important role in regu...
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Veröffentlicht in: | Theriogenology 2024-11, Vol.229, p.147-157 |
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description | Calcium ions (Ca2+) regulate cell proliferation and differentiation and participate in various physiological activities of cells. The calcium transfer protein inositol 1,4,5-triphosphate receptor (IP3R), located between the endoplasmic reticulum (ER) and mitochondria, plays an important role in regulating Ca2+ levels. However, the mechanism by which IP3R1 affects porcine meiotic progression and embryonic development remains unclear. We established a model in porcine oocytes using siRNA-mediated knockdown of IP3R1 to investigate the effects of IP3R1 on porcine oocyte meiotic progression and embryonic development. The results indicated that a decrease in IP3R1 expression significantly enhanced the interaction between the ER and mitochondria. Additionally, the interaction between the ER and the mitochondrial Ca2+ ([Ca2+]m) transport network protein IP3R1-GRP75-VDAC1 was disrupted. The results of the Duolink II in situ proximity ligation assay (PLA) revealed a weakened pairwise interaction between IP3R1-GRP75 and VDAC1 and a significantly increased interaction between GRP75 and VDAC1 after IP3R1 interference, resulting in the accumulation of large amounts of [Ca2+]m. These changes led to mitochondrial oxidative stress, increased the levels of reactive oxygen species (ROS) and reduced ATP production, which hindered the maturation and late development of porcine oocytes and induced apoptosis. Nevertheless, after treat with [Ca2+]m chelating agent ruthenium red (RR) or ROS scavenger N-acetylcysteine (NAC), the oocytes developmental abnormalities, oxidative stress and apoptosis caused by Ca2+ overload were improved. In conclusion, our results indicated IP3R1 is required for meiotic progression and embryonic development by regulating mitochondrial calcium and oxidative damage.
IP3R1 regulates the dynamic balance of mitochondrial calcium and the occurrence of oxidative damage by regulating the interaction between the mitochondria and the ER and the key IP3R1, GRP75 and VDAC1 factors, thus affecting the porcine oocytes meiotic progression and embryonic development. |
doi_str_mv | 10.1016/j.theriogenology.2024.08.023 |
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IP3R1 regulates the dynamic balance of mitochondrial calcium and the occurrence of oxidative damage by regulating the interaction between the mitochondria and the ER and the key IP3R1, GRP75 and VDAC1 factors, thus affecting the porcine oocytes meiotic progression and embryonic development.</description><identifier>ISSN: 0093-691X</identifier><identifier>ISSN: 1879-3231</identifier><identifier>EISSN: 1879-3231</identifier><identifier>DOI: 10.1016/j.theriogenology.2024.08.023</identifier><language>eng</language><publisher>Elsevier Inc</publisher><subject>Ca2 ; IP3R1 ; Mitochondria ; Porcine oocytes ; ROS</subject><ispartof>Theriogenology, 2024-11, Vol.229, p.147-157</ispartof><rights>2024 Elsevier Inc.</rights><rights>Copyright © 2024 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c288t-3acf58ee5d28defa14eb2bdfcb8482f0498b68176c3ff3fd6a3850e22b4227903</cites><orcidid>0000-0001-6669-5755</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.theriogenology.2024.08.023$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids></links><search><creatorcontrib>Zhang, Chang</creatorcontrib><creatorcontrib>Sun, Xiaoqing</creatorcontrib><creatorcontrib>Wu, Deyi</creatorcontrib><creatorcontrib>Wang, Guoxia</creatorcontrib><creatorcontrib>Lan, Hainan</creatorcontrib><creatorcontrib>Zheng, Xin</creatorcontrib><creatorcontrib>Li, Suo</creatorcontrib><title>IP3R1 is required for meiotic progression and embryonic development by regulating mitochondrial calcium and oxidative damage</title><title>Theriogenology</title><description>Calcium ions (Ca2+) regulate cell proliferation and differentiation and participate in various physiological activities of cells. The calcium transfer protein inositol 1,4,5-triphosphate receptor (IP3R), located between the endoplasmic reticulum (ER) and mitochondria, plays an important role in regulating Ca2+ levels. However, the mechanism by which IP3R1 affects porcine meiotic progression and embryonic development remains unclear. We established a model in porcine oocytes using siRNA-mediated knockdown of IP3R1 to investigate the effects of IP3R1 on porcine oocyte meiotic progression and embryonic development. The results indicated that a decrease in IP3R1 expression significantly enhanced the interaction between the ER and mitochondria. Additionally, the interaction between the ER and the mitochondrial Ca2+ ([Ca2+]m) transport network protein IP3R1-GRP75-VDAC1 was disrupted. The results of the Duolink II in situ proximity ligation assay (PLA) revealed a weakened pairwise interaction between IP3R1-GRP75 and VDAC1 and a significantly increased interaction between GRP75 and VDAC1 after IP3R1 interference, resulting in the accumulation of large amounts of [Ca2+]m. These changes led to mitochondrial oxidative stress, increased the levels of reactive oxygen species (ROS) and reduced ATP production, which hindered the maturation and late development of porcine oocytes and induced apoptosis. Nevertheless, after treat with [Ca2+]m chelating agent ruthenium red (RR) or ROS scavenger N-acetylcysteine (NAC), the oocytes developmental abnormalities, oxidative stress and apoptosis caused by Ca2+ overload were improved. In conclusion, our results indicated IP3R1 is required for meiotic progression and embryonic development by regulating mitochondrial calcium and oxidative damage.
IP3R1 regulates the dynamic balance of mitochondrial calcium and the occurrence of oxidative damage by regulating the interaction between the mitochondria and the ER and the key IP3R1, GRP75 and VDAC1 factors, thus affecting the porcine oocytes meiotic progression and embryonic development.</description><subject>Ca2</subject><subject>IP3R1</subject><subject>Mitochondria</subject><subject>Porcine oocytes</subject><subject>ROS</subject><issn>0093-691X</issn><issn>1879-3231</issn><issn>1879-3231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqNkEtr3DAUhUVIoZOk_0GLLLKxo4etkSGbEvKCQEtIoTshS1eOBluaSPbQgfz4KplusuvqLu75DpwPoXNKakqouNzU8wskHwcIcYzDvmaENTWRNWH8CK2oXHcVZ5weoxUhHa9ER39_RSc5bwghXAi6Qm8PP_kTxT7jBK-LT2CxiwlP4OPsDd6mOCTI2ceAdbAYpj7tYygfCzsY43aCMON-X-hhGfXsw4AnP0fzEoNNXo_Y6NH4Zfqg4x9vS2YH2OpJD3CGvjg9Zvj2756iX7c3z9f31eOPu4fr74-VYVLOFdfGtRKgtUxacJo20LPeOtPLRjJHmk72QtK1MNw57qzQXLYEGOsbxtYd4afo4tBb5rwukGc1-WxgHHWAuGTFSSeEaFvelOjVIWpSzDmBU9vkJ532ihL1Ll1t1Gfp6l26IlIV6QW_PeBQ5uw8JJWNh2DAFrVmVjb6_yv6CwMrl8E</recordid><startdate>202411</startdate><enddate>202411</enddate><creator>Zhang, Chang</creator><creator>Sun, Xiaoqing</creator><creator>Wu, Deyi</creator><creator>Wang, Guoxia</creator><creator>Lan, Hainan</creator><creator>Zheng, Xin</creator><creator>Li, Suo</creator><general>Elsevier Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6669-5755</orcidid></search><sort><creationdate>202411</creationdate><title>IP3R1 is required for meiotic progression and embryonic development by regulating mitochondrial calcium and oxidative damage</title><author>Zhang, Chang ; Sun, Xiaoqing ; Wu, Deyi ; Wang, Guoxia ; Lan, Hainan ; Zheng, Xin ; Li, Suo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c288t-3acf58ee5d28defa14eb2bdfcb8482f0498b68176c3ff3fd6a3850e22b4227903</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Ca2</topic><topic>IP3R1</topic><topic>Mitochondria</topic><topic>Porcine oocytes</topic><topic>ROS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Chang</creatorcontrib><creatorcontrib>Sun, Xiaoqing</creatorcontrib><creatorcontrib>Wu, Deyi</creatorcontrib><creatorcontrib>Wang, Guoxia</creatorcontrib><creatorcontrib>Lan, Hainan</creatorcontrib><creatorcontrib>Zheng, Xin</creatorcontrib><creatorcontrib>Li, Suo</creatorcontrib><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Theriogenology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Chang</au><au>Sun, Xiaoqing</au><au>Wu, Deyi</au><au>Wang, Guoxia</au><au>Lan, Hainan</au><au>Zheng, Xin</au><au>Li, Suo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>IP3R1 is required for meiotic progression and embryonic development by regulating mitochondrial calcium and oxidative damage</atitle><jtitle>Theriogenology</jtitle><date>2024-11</date><risdate>2024</risdate><volume>229</volume><spage>147</spage><epage>157</epage><pages>147-157</pages><issn>0093-691X</issn><issn>1879-3231</issn><eissn>1879-3231</eissn><abstract>Calcium ions (Ca2+) regulate cell proliferation and differentiation and participate in various physiological activities of cells. The calcium transfer protein inositol 1,4,5-triphosphate receptor (IP3R), located between the endoplasmic reticulum (ER) and mitochondria, plays an important role in regulating Ca2+ levels. However, the mechanism by which IP3R1 affects porcine meiotic progression and embryonic development remains unclear. We established a model in porcine oocytes using siRNA-mediated knockdown of IP3R1 to investigate the effects of IP3R1 on porcine oocyte meiotic progression and embryonic development. The results indicated that a decrease in IP3R1 expression significantly enhanced the interaction between the ER and mitochondria. Additionally, the interaction between the ER and the mitochondrial Ca2+ ([Ca2+]m) transport network protein IP3R1-GRP75-VDAC1 was disrupted. The results of the Duolink II in situ proximity ligation assay (PLA) revealed a weakened pairwise interaction between IP3R1-GRP75 and VDAC1 and a significantly increased interaction between GRP75 and VDAC1 after IP3R1 interference, resulting in the accumulation of large amounts of [Ca2+]m. These changes led to mitochondrial oxidative stress, increased the levels of reactive oxygen species (ROS) and reduced ATP production, which hindered the maturation and late development of porcine oocytes and induced apoptosis. Nevertheless, after treat with [Ca2+]m chelating agent ruthenium red (RR) or ROS scavenger N-acetylcysteine (NAC), the oocytes developmental abnormalities, oxidative stress and apoptosis caused by Ca2+ overload were improved. In conclusion, our results indicated IP3R1 is required for meiotic progression and embryonic development by regulating mitochondrial calcium and oxidative damage.
IP3R1 regulates the dynamic balance of mitochondrial calcium and the occurrence of oxidative damage by regulating the interaction between the mitochondria and the ER and the key IP3R1, GRP75 and VDAC1 factors, thus affecting the porcine oocytes meiotic progression and embryonic development.</abstract><pub>Elsevier Inc</pub><doi>10.1016/j.theriogenology.2024.08.023</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0001-6669-5755</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Ca2 IP3R1 Mitochondria Porcine oocytes ROS |
title | IP3R1 is required for meiotic progression and embryonic development by regulating mitochondrial calcium and oxidative damage |
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