Microbicidal Polymer Nanoparticles Containing Clotrimazole for Treatment of Vulvovaginal Candidiasis
Vulvovaginal candidiasis (VVC) alters the innate cervicovaginal immunity, which provides an important barrier against viruses and other infections. The incidence of this disease has not decreased in the last 30 years, so effective treatments are still needed. Nanoparticles (NPs) of cellulose acetate...
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| Veröffentlicht in: | AAPS PharmSciTech 2024-08, Vol.25 (7), p.197, Article 197 |
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| creator | del Rocío Lara-Sánchez, María Ganem-Rondero, Adriana Nava-Arzaluz, María Guadalupe Becerril-Osnaya, Andrea Angela Pérez-Carranza, Laura Abril Alcalá-Alcalá, Sergio Mendoza-Muñoz, Néstor Piñón-Segundo, Elizabeth |
| description | Vulvovaginal candidiasis (VVC) alters the innate cervicovaginal immunity, which provides an important barrier against viruses and other infections. The incidence of this disease has not decreased in the last 30 years, so effective treatments are still needed. Nanoparticles (NPs) of cellulose acetate phthalate (CAP) and clotrimazole (CLZ) were prepared by the emulsification-diffusion method. NPs were characterized using dynamic light scattering, atomic force microscopy and differential scanning calorimetry; their release profile was determined by the dialysis bag technique and mucoadhesion was evaluated with the mucin-particle method. The growth inhibition study of
Candida albicans
was carried out using the plate counting technique. Finally, accelerated physical stability tests of NPs were carried out, both in water and in SVF. The CAP-CLZ NPs had an average diameter of 273.4 nm, a PDI of 0.284, smooth surfaces and spherical shapes.
In vitro
release of CLZ from the CAP NPs was categorized with the Weibull model as a matrix system in which initial release was rapid and subsequently sustained. The inhibition of
C. albicans
growth by the CAP-CLZ NPs was greater than that of free CLZ, and the CAP-only NPs had a microbicidal effect on
C. albicans
. The NPs showed poor mucoadhesiveness, which could lead to studies of their mucopenetration capacities. An accelerated physical stability test revealed the erosion of CAP in aqueous media. A nanoparticulate system was developed and provided sustained release of CLZ, and it combined an antifungal agent with a microbial polymer that exhibited antifungal activity against
C. albicans
.
Graphical Abstract |
| doi_str_mv | 10.1208/s12249-024-02914-7 |
| format | Article |
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Candida albicans
was carried out using the plate counting technique. Finally, accelerated physical stability tests of NPs were carried out, both in water and in SVF. The CAP-CLZ NPs had an average diameter of 273.4 nm, a PDI of 0.284, smooth surfaces and spherical shapes.
In vitro
release of CLZ from the CAP NPs was categorized with the Weibull model as a matrix system in which initial release was rapid and subsequently sustained. The inhibition of
C. albicans
growth by the CAP-CLZ NPs was greater than that of free CLZ, and the CAP-only NPs had a microbicidal effect on
C. albicans
. The NPs showed poor mucoadhesiveness, which could lead to studies of their mucopenetration capacities. An accelerated physical stability test revealed the erosion of CAP in aqueous media. A nanoparticulate system was developed and provided sustained release of CLZ, and it combined an antifungal agent with a microbial polymer that exhibited antifungal activity against
C. albicans
.
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Candida albicans
was carried out using the plate counting technique. Finally, accelerated physical stability tests of NPs were carried out, both in water and in SVF. The CAP-CLZ NPs had an average diameter of 273.4 nm, a PDI of 0.284, smooth surfaces and spherical shapes.
In vitro
release of CLZ from the CAP NPs was categorized with the Weibull model as a matrix system in which initial release was rapid and subsequently sustained. The inhibition of
C. albicans
growth by the CAP-CLZ NPs was greater than that of free CLZ, and the CAP-only NPs had a microbicidal effect on
C. albicans
. The NPs showed poor mucoadhesiveness, which could lead to studies of their mucopenetration capacities. An accelerated physical stability test revealed the erosion of CAP in aqueous media. A nanoparticulate system was developed and provided sustained release of CLZ, and it combined an antifungal agent with a microbial polymer that exhibited antifungal activity against
C. albicans
.
Graphical Abstract</description><subject>Antifungal Agents - administration & dosage</subject><subject>Antifungal Agents - pharmacology</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>Candida albicans - drug effects</subject><subject>Candidiasis, Vulvovaginal - drug therapy</subject><subject>Cellulose - analogs & derivatives</subject><subject>Cellulose - chemistry</subject><subject>Clotrimazole - administration & dosage</subject><subject>Clotrimazole - pharmacology</subject><subject>Drug Liberation</subject><subject>Female</subject><subject>Microbial Sensitivity Tests - methods</subject><subject>Nanoparticles - chemistry</subject><subject>Particle Size</subject><subject>Pharmacology/Toxicology</subject><subject>Pharmacy</subject><subject>Polymers - chemistry</subject><subject>Research Article</subject><issn>1530-9932</issn><issn>1530-9932</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><recordid>eNp9kEtLxDAUhYMovv-AC-nSTTUvm2YpxRf4WqjbcJumQyRNxqQVxl9vxhnFlYvLvXDPOXA-hI4IPiUU12eJUMpliSnPIwkvxQbaJecMl1Iyuvnn3kF7Kb1hTBmRbBvtMEkEF5juou7e6hhaq20HrngKbjGYWDyAD3OIo9XOpKIJfgTrrZ8VjQtjtAN8BmeKPsTiORoYB-PHIvTF6-Q-wgfMrM9ZDfjOdhaSTQdoqweXzOF676OXq8vn5qa8e7y-bS7uSk0FHUsJxtSmI6aXuiW4pzWtWcs4riUT0PagKddSAxfteddXTLTAdMU5iApAMmD76GSVO4_hfTJpVINN2jgH3oQpKYZlRWvMaJWldCXN7VOKplfzZa-4UASrJV21oqsyXfVNV4lsOl7nT-1gul_LD84sYCtByi8_M1G9hSlmGum_2C8y7IfQ</recordid><startdate>20240822</startdate><enddate>20240822</enddate><creator>del Rocío Lara-Sánchez, María</creator><creator>Ganem-Rondero, Adriana</creator><creator>Nava-Arzaluz, María Guadalupe</creator><creator>Becerril-Osnaya, Andrea Angela</creator><creator>Pérez-Carranza, Laura Abril</creator><creator>Alcalá-Alcalá, Sergio</creator><creator>Mendoza-Muñoz, Néstor</creator><creator>Piñón-Segundo, Elizabeth</creator><general>Springer International Publishing</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-4172-6233</orcidid></search><sort><creationdate>20240822</creationdate><title>Microbicidal Polymer Nanoparticles Containing Clotrimazole for Treatment of Vulvovaginal Candidiasis</title><author>del Rocío Lara-Sánchez, María ; Ganem-Rondero, Adriana ; Nava-Arzaluz, María Guadalupe ; Becerril-Osnaya, Andrea Angela ; Pérez-Carranza, Laura Abril ; Alcalá-Alcalá, Sergio ; Mendoza-Muñoz, Néstor ; Piñón-Segundo, Elizabeth</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c272t-9aee8ed1ef9cb10f28283b3408937abfac24c9ca47b5df637ba3c644a76aa93a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Antifungal Agents - administration & dosage</topic><topic>Antifungal Agents - pharmacology</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Biotechnology</topic><topic>Candida albicans - drug effects</topic><topic>Candidiasis, Vulvovaginal - drug therapy</topic><topic>Cellulose - analogs & derivatives</topic><topic>Cellulose - chemistry</topic><topic>Clotrimazole - administration & dosage</topic><topic>Clotrimazole - pharmacology</topic><topic>Drug Liberation</topic><topic>Female</topic><topic>Microbial Sensitivity Tests - methods</topic><topic>Nanoparticles - chemistry</topic><topic>Particle Size</topic><topic>Pharmacology/Toxicology</topic><topic>Pharmacy</topic><topic>Polymers - chemistry</topic><topic>Research Article</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>del Rocío Lara-Sánchez, María</creatorcontrib><creatorcontrib>Ganem-Rondero, Adriana</creatorcontrib><creatorcontrib>Nava-Arzaluz, María Guadalupe</creatorcontrib><creatorcontrib>Becerril-Osnaya, Andrea Angela</creatorcontrib><creatorcontrib>Pérez-Carranza, Laura Abril</creatorcontrib><creatorcontrib>Alcalá-Alcalá, Sergio</creatorcontrib><creatorcontrib>Mendoza-Muñoz, Néstor</creatorcontrib><creatorcontrib>Piñón-Segundo, Elizabeth</creatorcontrib><collection>Springer Nature OA/Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>AAPS PharmSciTech</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>del Rocío Lara-Sánchez, María</au><au>Ganem-Rondero, Adriana</au><au>Nava-Arzaluz, María Guadalupe</au><au>Becerril-Osnaya, Andrea Angela</au><au>Pérez-Carranza, Laura Abril</au><au>Alcalá-Alcalá, Sergio</au><au>Mendoza-Muñoz, Néstor</au><au>Piñón-Segundo, Elizabeth</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microbicidal Polymer Nanoparticles Containing Clotrimazole for Treatment of Vulvovaginal Candidiasis</atitle><jtitle>AAPS PharmSciTech</jtitle><stitle>AAPS PharmSciTech</stitle><addtitle>AAPS PharmSciTech</addtitle><date>2024-08-22</date><risdate>2024</risdate><volume>25</volume><issue>7</issue><spage>197</spage><pages>197-</pages><artnum>197</artnum><issn>1530-9932</issn><eissn>1530-9932</eissn><abstract>Vulvovaginal candidiasis (VVC) alters the innate cervicovaginal immunity, which provides an important barrier against viruses and other infections. The incidence of this disease has not decreased in the last 30 years, so effective treatments are still needed. Nanoparticles (NPs) of cellulose acetate phthalate (CAP) and clotrimazole (CLZ) were prepared by the emulsification-diffusion method. NPs were characterized using dynamic light scattering, atomic force microscopy and differential scanning calorimetry; their release profile was determined by the dialysis bag technique and mucoadhesion was evaluated with the mucin-particle method. The growth inhibition study of
Candida albicans
was carried out using the plate counting technique. Finally, accelerated physical stability tests of NPs were carried out, both in water and in SVF. The CAP-CLZ NPs had an average diameter of 273.4 nm, a PDI of 0.284, smooth surfaces and spherical shapes.
In vitro
release of CLZ from the CAP NPs was categorized with the Weibull model as a matrix system in which initial release was rapid and subsequently sustained. The inhibition of
C. albicans
growth by the CAP-CLZ NPs was greater than that of free CLZ, and the CAP-only NPs had a microbicidal effect on
C. albicans
. The NPs showed poor mucoadhesiveness, which could lead to studies of their mucopenetration capacities. An accelerated physical stability test revealed the erosion of CAP in aqueous media. A nanoparticulate system was developed and provided sustained release of CLZ, and it combined an antifungal agent with a microbial polymer that exhibited antifungal activity against
C. albicans
.
Graphical Abstract</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>39174702</pmid><doi>10.1208/s12249-024-02914-7</doi><orcidid>https://orcid.org/0000-0002-4172-6233</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Antifungal Agents - administration & dosage Antifungal Agents - pharmacology Biochemistry Biomedical and Life Sciences Biomedicine Biotechnology Candida albicans - drug effects Candidiasis, Vulvovaginal - drug therapy Cellulose - analogs & derivatives Cellulose - chemistry Clotrimazole - administration & dosage Clotrimazole - pharmacology Drug Liberation Female Microbial Sensitivity Tests - methods Nanoparticles - chemistry Particle Size Pharmacology/Toxicology Pharmacy Polymers - chemistry Research Article |
| title | Microbicidal Polymer Nanoparticles Containing Clotrimazole for Treatment of Vulvovaginal Candidiasis |
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