Fluorescent probes for neuroscience: imaging ex vivo brain tissue sections
Neurobiological research relies heavily on imaging techniques, such as fluorescence microscopy, to understand neurological function and disease processes. However, the number and variety of fluorescent probes available for tissue section imaging limits the advance of research in the field. In this r...
Gespeichert in:
| Veröffentlicht in: | Analyst (London) 2024-09, Vol.149 (18), p.4536-4552 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 4552 |
|---|---|
| container_issue | 18 |
| container_start_page | 4536 |
| container_title | Analyst (London) |
| container_volume | 149 |
| creator | Schwehr, Bradley J Hartnell, David Ellison, Gaewyn Hindes, Madison T Milford, Breah Dallerba, Elena Hickey, Shane M Pfeffer, Frederick M Brooks, Doug A Massi, Massimiliano Hackett, Mark J |
| description | Neurobiological research relies heavily on imaging techniques, such as fluorescence microscopy, to understand neurological function and disease processes. However, the number and variety of fluorescent probes available for
tissue section imaging limits the advance of research in the field. In this review, we outline the current range of fluorescent probes that are available to researchers for
brain section imaging, including their physical and chemical characteristics, staining targets, and examples of discoveries for which they have been used. This review is organised into sections based on the biological target of the probe, including subcellular organelles, chemical species (
, labile metal ions), and pathological phenomenon (
, degenerating cells, aggregated proteins). We hope to inspire further development in this field, given the considerable benefits to be gained by the greater availability of suitably sensitive probes that have specificity for important brain tissue targets. |
| doi_str_mv | 10.1039/d4an00663a |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3095682555</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3101938635</sourcerecordid><originalsourceid>FETCH-LOGICAL-c204t-143b4cc9bfb4604a1c00f235c1c186a9a1f4d19d57f3081dc2f18cf88138c3113</originalsourceid><addsrcrecordid>eNpdkEtLw0AUhQdRbK1u_AEy4EaE6L2ZRyfuSrU-KLrRdZhMZkpKOqkzSdF_b2rVhavLhY_DOR8hpwhXCCy7Lrn2AFIyvUeGyCRPhEjVPhkCAEtSKfiAHMW47F8EAYdkwDIco8TxkDzN6q4JNhrrW7oOTWEjdU2g3nahiaay3tgbWq30ovILaj_opto0tAi68rStYuwsjda0VePjMTlwuo725OeOyNvs7nX6kMxf7h-nk3liUuBtgpwV3JiscAWXwDUaAJcyYdCgkjrT6HiJWSnGjoHC0qQOlXFKIVOGIbIRudjl9nXfOxvbfFX1_etae9t0MWeQCalSIUSPnv9Dl00XfN8uZwiYMSXZlrrcUaafHIN1-Tr0i8NnjpBvDee3fPL8bXjSw2c_kV2xsuUf-quUfQH1WXUR</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3101938635</pqid></control><display><type>article</type><title>Fluorescent probes for neuroscience: imaging ex vivo brain tissue sections</title><source>Royal Society of Chemistry Journals Archive (1841-2007)</source><source>MEDLINE</source><source>Royal Society Of Chemistry Journals 2008-</source><source>Alma/SFX Local Collection</source><creator>Schwehr, Bradley J ; Hartnell, David ; Ellison, Gaewyn ; Hindes, Madison T ; Milford, Breah ; Dallerba, Elena ; Hickey, Shane M ; Pfeffer, Frederick M ; Brooks, Doug A ; Massi, Massimiliano ; Hackett, Mark J</creator><creatorcontrib>Schwehr, Bradley J ; Hartnell, David ; Ellison, Gaewyn ; Hindes, Madison T ; Milford, Breah ; Dallerba, Elena ; Hickey, Shane M ; Pfeffer, Frederick M ; Brooks, Doug A ; Massi, Massimiliano ; Hackett, Mark J</creatorcontrib><description>Neurobiological research relies heavily on imaging techniques, such as fluorescence microscopy, to understand neurological function and disease processes. However, the number and variety of fluorescent probes available for
tissue section imaging limits the advance of research in the field. In this review, we outline the current range of fluorescent probes that are available to researchers for
brain section imaging, including their physical and chemical characteristics, staining targets, and examples of discoveries for which they have been used. This review is organised into sections based on the biological target of the probe, including subcellular organelles, chemical species (
, labile metal ions), and pathological phenomenon (
, degenerating cells, aggregated proteins). We hope to inspire further development in this field, given the considerable benefits to be gained by the greater availability of suitably sensitive probes that have specificity for important brain tissue targets.</description><identifier>ISSN: 0003-2654</identifier><identifier>ISSN: 1364-5528</identifier><identifier>EISSN: 1364-5528</identifier><identifier>DOI: 10.1039/d4an00663a</identifier><identifier>PMID: 39171617</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Animals ; Availability ; Brain ; Brain - diagnostic imaging ; Fluorescent Dyes - chemistry ; Fluorescent indicators ; Humans ; Imaging techniques ; Medical imaging ; Microscopy, Fluorescence - methods ; Neurosciences - methods ; Organelles</subject><ispartof>Analyst (London), 2024-09, Vol.149 (18), p.4536-4552</ispartof><rights>Copyright Royal Society of Chemistry 2024</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c204t-143b4cc9bfb4604a1c00f235c1c186a9a1f4d19d57f3081dc2f18cf88138c3113</cites><orcidid>0000-0001-9750-3250 ; 0000-0002-3063-531X ; 0000-0001-9259-4106 ; 0000-0001-9098-3626 ; 0000-0002-3296-7270 ; 0000-0002-5382-4519 ; 0009-0002-8117-5672 ; 0000-0002-5441-6437 ; 0000-0001-6949-4019</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,2818,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39171617$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schwehr, Bradley J</creatorcontrib><creatorcontrib>Hartnell, David</creatorcontrib><creatorcontrib>Ellison, Gaewyn</creatorcontrib><creatorcontrib>Hindes, Madison T</creatorcontrib><creatorcontrib>Milford, Breah</creatorcontrib><creatorcontrib>Dallerba, Elena</creatorcontrib><creatorcontrib>Hickey, Shane M</creatorcontrib><creatorcontrib>Pfeffer, Frederick M</creatorcontrib><creatorcontrib>Brooks, Doug A</creatorcontrib><creatorcontrib>Massi, Massimiliano</creatorcontrib><creatorcontrib>Hackett, Mark J</creatorcontrib><title>Fluorescent probes for neuroscience: imaging ex vivo brain tissue sections</title><title>Analyst (London)</title><addtitle>Analyst</addtitle><description>Neurobiological research relies heavily on imaging techniques, such as fluorescence microscopy, to understand neurological function and disease processes. However, the number and variety of fluorescent probes available for
tissue section imaging limits the advance of research in the field. In this review, we outline the current range of fluorescent probes that are available to researchers for
brain section imaging, including their physical and chemical characteristics, staining targets, and examples of discoveries for which they have been used. This review is organised into sections based on the biological target of the probe, including subcellular organelles, chemical species (
, labile metal ions), and pathological phenomenon (
, degenerating cells, aggregated proteins). We hope to inspire further development in this field, given the considerable benefits to be gained by the greater availability of suitably sensitive probes that have specificity for important brain tissue targets.</description><subject>Animals</subject><subject>Availability</subject><subject>Brain</subject><subject>Brain - diagnostic imaging</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Fluorescent indicators</subject><subject>Humans</subject><subject>Imaging techniques</subject><subject>Medical imaging</subject><subject>Microscopy, Fluorescence - methods</subject><subject>Neurosciences - methods</subject><subject>Organelles</subject><issn>0003-2654</issn><issn>1364-5528</issn><issn>1364-5528</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkEtLw0AUhQdRbK1u_AEy4EaE6L2ZRyfuSrU-KLrRdZhMZkpKOqkzSdF_b2rVhavLhY_DOR8hpwhXCCy7Lrn2AFIyvUeGyCRPhEjVPhkCAEtSKfiAHMW47F8EAYdkwDIco8TxkDzN6q4JNhrrW7oOTWEjdU2g3nahiaay3tgbWq30ovILaj_opto0tAi68rStYuwsjda0VePjMTlwuo725OeOyNvs7nX6kMxf7h-nk3liUuBtgpwV3JiscAWXwDUaAJcyYdCgkjrT6HiJWSnGjoHC0qQOlXFKIVOGIbIRudjl9nXfOxvbfFX1_etae9t0MWeQCalSIUSPnv9Dl00XfN8uZwiYMSXZlrrcUaafHIN1-Tr0i8NnjpBvDee3fPL8bXjSw2c_kV2xsuUf-quUfQH1WXUR</recordid><startdate>20240909</startdate><enddate>20240909</enddate><creator>Schwehr, Bradley J</creator><creator>Hartnell, David</creator><creator>Ellison, Gaewyn</creator><creator>Hindes, Madison T</creator><creator>Milford, Breah</creator><creator>Dallerba, Elena</creator><creator>Hickey, Shane M</creator><creator>Pfeffer, Frederick M</creator><creator>Brooks, Doug A</creator><creator>Massi, Massimiliano</creator><creator>Hackett, Mark J</creator><general>Royal Society of Chemistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-9750-3250</orcidid><orcidid>https://orcid.org/0000-0002-3063-531X</orcidid><orcidid>https://orcid.org/0000-0001-9259-4106</orcidid><orcidid>https://orcid.org/0000-0001-9098-3626</orcidid><orcidid>https://orcid.org/0000-0002-3296-7270</orcidid><orcidid>https://orcid.org/0000-0002-5382-4519</orcidid><orcidid>https://orcid.org/0009-0002-8117-5672</orcidid><orcidid>https://orcid.org/0000-0002-5441-6437</orcidid><orcidid>https://orcid.org/0000-0001-6949-4019</orcidid></search><sort><creationdate>20240909</creationdate><title>Fluorescent probes for neuroscience: imaging ex vivo brain tissue sections</title><author>Schwehr, Bradley J ; Hartnell, David ; Ellison, Gaewyn ; Hindes, Madison T ; Milford, Breah ; Dallerba, Elena ; Hickey, Shane M ; Pfeffer, Frederick M ; Brooks, Doug A ; Massi, Massimiliano ; Hackett, Mark J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c204t-143b4cc9bfb4604a1c00f235c1c186a9a1f4d19d57f3081dc2f18cf88138c3113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Animals</topic><topic>Availability</topic><topic>Brain</topic><topic>Brain - diagnostic imaging</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Fluorescent indicators</topic><topic>Humans</topic><topic>Imaging techniques</topic><topic>Medical imaging</topic><topic>Microscopy, Fluorescence - methods</topic><topic>Neurosciences - methods</topic><topic>Organelles</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schwehr, Bradley J</creatorcontrib><creatorcontrib>Hartnell, David</creatorcontrib><creatorcontrib>Ellison, Gaewyn</creatorcontrib><creatorcontrib>Hindes, Madison T</creatorcontrib><creatorcontrib>Milford, Breah</creatorcontrib><creatorcontrib>Dallerba, Elena</creatorcontrib><creatorcontrib>Hickey, Shane M</creatorcontrib><creatorcontrib>Pfeffer, Frederick M</creatorcontrib><creatorcontrib>Brooks, Doug A</creatorcontrib><creatorcontrib>Massi, Massimiliano</creatorcontrib><creatorcontrib>Hackett, Mark J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Analyst (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schwehr, Bradley J</au><au>Hartnell, David</au><au>Ellison, Gaewyn</au><au>Hindes, Madison T</au><au>Milford, Breah</au><au>Dallerba, Elena</au><au>Hickey, Shane M</au><au>Pfeffer, Frederick M</au><au>Brooks, Doug A</au><au>Massi, Massimiliano</au><au>Hackett, Mark J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fluorescent probes for neuroscience: imaging ex vivo brain tissue sections</atitle><jtitle>Analyst (London)</jtitle><addtitle>Analyst</addtitle><date>2024-09-09</date><risdate>2024</risdate><volume>149</volume><issue>18</issue><spage>4536</spage><epage>4552</epage><pages>4536-4552</pages><issn>0003-2654</issn><issn>1364-5528</issn><eissn>1364-5528</eissn><abstract>Neurobiological research relies heavily on imaging techniques, such as fluorescence microscopy, to understand neurological function and disease processes. However, the number and variety of fluorescent probes available for
tissue section imaging limits the advance of research in the field. In this review, we outline the current range of fluorescent probes that are available to researchers for
brain section imaging, including their physical and chemical characteristics, staining targets, and examples of discoveries for which they have been used. This review is organised into sections based on the biological target of the probe, including subcellular organelles, chemical species (
, labile metal ions), and pathological phenomenon (
, degenerating cells, aggregated proteins). We hope to inspire further development in this field, given the considerable benefits to be gained by the greater availability of suitably sensitive probes that have specificity for important brain tissue targets.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>39171617</pmid><doi>10.1039/d4an00663a</doi><tpages>17</tpages><orcidid>https://orcid.org/0000-0001-9750-3250</orcidid><orcidid>https://orcid.org/0000-0002-3063-531X</orcidid><orcidid>https://orcid.org/0000-0001-9259-4106</orcidid><orcidid>https://orcid.org/0000-0001-9098-3626</orcidid><orcidid>https://orcid.org/0000-0002-3296-7270</orcidid><orcidid>https://orcid.org/0000-0002-5382-4519</orcidid><orcidid>https://orcid.org/0009-0002-8117-5672</orcidid><orcidid>https://orcid.org/0000-0002-5441-6437</orcidid><orcidid>https://orcid.org/0000-0001-6949-4019</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2654 |
| ispartof | Analyst (London), 2024-09, Vol.149 (18), p.4536-4552 |
| issn | 0003-2654 1364-5528 1364-5528 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_3095682555 |
| source | Royal Society of Chemistry Journals Archive (1841-2007); MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Animals Availability Brain Brain - diagnostic imaging Fluorescent Dyes - chemistry Fluorescent indicators Humans Imaging techniques Medical imaging Microscopy, Fluorescence - methods Neurosciences - methods Organelles |
| title | Fluorescent probes for neuroscience: imaging ex vivo brain tissue sections |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-11T23%3A21%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Fluorescent%20probes%20for%20neuroscience:%20imaging%20ex%20vivo%20brain%20tissue%20sections&rft.jtitle=Analyst%20(London)&rft.au=Schwehr,%20Bradley%20J&rft.date=2024-09-09&rft.volume=149&rft.issue=18&rft.spage=4536&rft.epage=4552&rft.pages=4536-4552&rft.issn=0003-2654&rft.eissn=1364-5528&rft_id=info:doi/10.1039/d4an00663a&rft_dat=%3Cproquest_cross%3E3101938635%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3101938635&rft_id=info:pmid/39171617&rfr_iscdi=true |