Characterization of emetic Bacillus cereus biofilm formation and cereulide production in biofilm
[Display omitted] •Dynamic processes of biofilm formation and cereulide production under four different conditions.•The substrate has an impact on the formation of biofilms and cereulide of Bacillus cereus.•The cereulide levels in biofilms formed in BHI medium are mostly higher than those formed in...
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| Veröffentlicht in: | Food research international 2024-09, Vol.192, p.114834, Article 114834 |
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| creator | Ren, Fanchong Chen, Yuhang Yang, Shuo Zhang, Yinan Liu, Yangtai Ma, Yue Wang, Yating Liu, Yang Dong, Qingli Lu, Dasheng |
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•Dynamic processes of biofilm formation and cereulide production under four different conditions.•The substrate has an impact on the formation of biofilms and cereulide of Bacillus cereus.•The cereulide levels in biofilms formed in BHI medium are mostly higher than those formed in UHT milk.
Bacillus cereus is a well-known foodborne pathogen that can cause human diseases, including vomiting caused by emetic toxin, cereulide, requiring 105-108 cells per gram to cause the disease. The bacterial cells may be eliminated during processing, but cereulide can survive in most processing techniques due to its resistance to high temperatures, extreme pH and proteolytic enzymes. Herein, we reported dynamic processes of biofilm formation of four different types and cereulide production within the biofilm. Confocal laser scanning microscopy (CLSM) images revealed that biofilms of the four different types reach each stage at different time points. Among the extracellular polymeric substances (EPS) components of the four biofilms formed by the emetic B. cereus F4810/72 strain, proteins account for the majority. In addition, there are significant differences (p |
| doi_str_mv | 10.1016/j.foodres.2024.114834 |
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•Dynamic processes of biofilm formation and cereulide production under four different conditions.•The substrate has an impact on the formation of biofilms and cereulide of Bacillus cereus.•The cereulide levels in biofilms formed in BHI medium are mostly higher than those formed in UHT milk.
Bacillus cereus is a well-known foodborne pathogen that can cause human diseases, including vomiting caused by emetic toxin, cereulide, requiring 105-108 cells per gram to cause the disease. The bacterial cells may be eliminated during processing, but cereulide can survive in most processing techniques due to its resistance to high temperatures, extreme pH and proteolytic enzymes. Herein, we reported dynamic processes of biofilm formation of four different types and cereulide production within the biofilm. Confocal laser scanning microscopy (CLSM) images revealed that biofilms of the four different types reach each stage at different time points. Among the extracellular polymeric substances (EPS) components of the four biofilms formed by the emetic B. cereus F4810/72 strain, proteins account for the majority. In addition, there are significant differences (p < 0.05) in the EPS components at the same stage among biofilms of different types. The time point at which cereulide was first detected in the four types of biofilms was 24 h. In the biofilm of B. cereus formed in ultra-high-temperature (UHT) milk, the first peak of cereulide appeared at 72 h. The cereulide content of the biofilms formed in BHI was mostly higher than that of the biofilms formed in UHT milk. This study contributes to a better understanding of food safety issues in the industry caused by biofilm and cereulide toxin produced by B. cereus.</description><identifier>ISSN: 0963-9969</identifier><identifier>ISSN: 1873-7145</identifier><identifier>EISSN: 1873-7145</identifier><identifier>DOI: 10.1016/j.foodres.2024.114834</identifier><identifier>PMID: 39147521</identifier><language>eng</language><publisher>Canada: Elsevier Ltd</publisher><subject>Animals ; Bacillus cereus ; Bacillus cereus - metabolism ; Bacillus cereus - physiology ; Biofilm ; Biofilms - growth & development ; Cereulide ; CLSM ; Depsipeptides - metabolism ; EPS ; Extracellular Polymeric Substance Matrix - metabolism ; Food Handling - methods ; Food Microbiology ; Foodborne Diseases - microbiology ; Hot Temperature ; Microscopy, Confocal ; Milk - microbiology</subject><ispartof>Food research international, 2024-09, Vol.192, p.114834, Article 114834</ispartof><rights>2024 Elsevier Ltd</rights><rights>Copyright © 2024 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c243t-75382c82b6f37dbafa835cdb13d70d6299b4ce1b9a1dda11ab2a77d7cf8991aa3</cites><orcidid>0000-0001-6352-4002 ; 0000-0001-6981-0878 ; 0000-0002-5665-034X ; 0009-0003-6730-0016 ; 0000-0003-2409-8666 ; 0000-0001-9019-0001 ; 0009-0004-8204-7626 ; 0000-0001-6961-3874</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.foodres.2024.114834$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39147521$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ren, Fanchong</creatorcontrib><creatorcontrib>Chen, Yuhang</creatorcontrib><creatorcontrib>Yang, Shuo</creatorcontrib><creatorcontrib>Zhang, Yinan</creatorcontrib><creatorcontrib>Liu, Yangtai</creatorcontrib><creatorcontrib>Ma, Yue</creatorcontrib><creatorcontrib>Wang, Yating</creatorcontrib><creatorcontrib>Liu, Yang</creatorcontrib><creatorcontrib>Dong, Qingli</creatorcontrib><creatorcontrib>Lu, Dasheng</creatorcontrib><title>Characterization of emetic Bacillus cereus biofilm formation and cereulide production in biofilm</title><title>Food research international</title><addtitle>Food Res Int</addtitle><description>[Display omitted]
•Dynamic processes of biofilm formation and cereulide production under four different conditions.•The substrate has an impact on the formation of biofilms and cereulide of Bacillus cereus.•The cereulide levels in biofilms formed in BHI medium are mostly higher than those formed in UHT milk.
Bacillus cereus is a well-known foodborne pathogen that can cause human diseases, including vomiting caused by emetic toxin, cereulide, requiring 105-108 cells per gram to cause the disease. The bacterial cells may be eliminated during processing, but cereulide can survive in most processing techniques due to its resistance to high temperatures, extreme pH and proteolytic enzymes. Herein, we reported dynamic processes of biofilm formation of four different types and cereulide production within the biofilm. Confocal laser scanning microscopy (CLSM) images revealed that biofilms of the four different types reach each stage at different time points. Among the extracellular polymeric substances (EPS) components of the four biofilms formed by the emetic B. cereus F4810/72 strain, proteins account for the majority. In addition, there are significant differences (p < 0.05) in the EPS components at the same stage among biofilms of different types. The time point at which cereulide was first detected in the four types of biofilms was 24 h. In the biofilm of B. cereus formed in ultra-high-temperature (UHT) milk, the first peak of cereulide appeared at 72 h. The cereulide content of the biofilms formed in BHI was mostly higher than that of the biofilms formed in UHT milk. This study contributes to a better understanding of food safety issues in the industry caused by biofilm and cereulide toxin produced by B. cereus.</description><subject>Animals</subject><subject>Bacillus cereus</subject><subject>Bacillus cereus - metabolism</subject><subject>Bacillus cereus - physiology</subject><subject>Biofilm</subject><subject>Biofilms - growth & development</subject><subject>Cereulide</subject><subject>CLSM</subject><subject>Depsipeptides - metabolism</subject><subject>EPS</subject><subject>Extracellular Polymeric Substance Matrix - metabolism</subject><subject>Food Handling - methods</subject><subject>Food Microbiology</subject><subject>Foodborne Diseases - microbiology</subject><subject>Hot Temperature</subject><subject>Microscopy, Confocal</subject><subject>Milk - microbiology</subject><issn>0963-9969</issn><issn>1873-7145</issn><issn>1873-7145</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkLtOwzAUhi0EoqXwCKCMLAm-JHE8Iai4SZVYYDaOfSxcJXGxEyR4elLSsjKd4Xz_uXwInROcEUzKq3VmvTcBYkYxzTNC8orlB2hOKs5STvLiEM2xKFkqRClm6CTGNca4LLg4RjMmSM4LSubobfmugtI9BPeteue7xNsEWuidTm6Vdk0zxERDgLHUzlvXtIn1oZ1Y1Zmp2TgDySZ4M-jfhuv29Ck6sqqJcLarC_R6f_eyfExXzw9Py5tVqmnO-pQXrKK6onVpGTe1sqpihTY1YYZjU1Ih6lwDqYUixihCVE0V54ZrWwlBlGILdDnNHa_4GCD2snVRQ9OoDvwQJcOCFYLhCo9oMaE6-BgDWLkJrlXhSxIst3LlWu7kyq1cOckdcxe7FUPdgvlL7W2OwPUEwPjop4Mgo3bQaTAugO6l8e6fFT8gk4_o</recordid><startdate>202409</startdate><enddate>202409</enddate><creator>Ren, Fanchong</creator><creator>Chen, Yuhang</creator><creator>Yang, Shuo</creator><creator>Zhang, Yinan</creator><creator>Liu, Yangtai</creator><creator>Ma, Yue</creator><creator>Wang, Yating</creator><creator>Liu, Yang</creator><creator>Dong, Qingli</creator><creator>Lu, Dasheng</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6352-4002</orcidid><orcidid>https://orcid.org/0000-0001-6981-0878</orcidid><orcidid>https://orcid.org/0000-0002-5665-034X</orcidid><orcidid>https://orcid.org/0009-0003-6730-0016</orcidid><orcidid>https://orcid.org/0000-0003-2409-8666</orcidid><orcidid>https://orcid.org/0000-0001-9019-0001</orcidid><orcidid>https://orcid.org/0009-0004-8204-7626</orcidid><orcidid>https://orcid.org/0000-0001-6961-3874</orcidid></search><sort><creationdate>202409</creationdate><title>Characterization of emetic Bacillus cereus biofilm formation and cereulide production in biofilm</title><author>Ren, Fanchong ; Chen, Yuhang ; Yang, Shuo ; Zhang, Yinan ; Liu, Yangtai ; Ma, Yue ; Wang, Yating ; Liu, Yang ; Dong, Qingli ; Lu, Dasheng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c243t-75382c82b6f37dbafa835cdb13d70d6299b4ce1b9a1dda11ab2a77d7cf8991aa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Animals</topic><topic>Bacillus cereus</topic><topic>Bacillus cereus - metabolism</topic><topic>Bacillus cereus - physiology</topic><topic>Biofilm</topic><topic>Biofilms - growth & development</topic><topic>Cereulide</topic><topic>CLSM</topic><topic>Depsipeptides - metabolism</topic><topic>EPS</topic><topic>Extracellular Polymeric Substance Matrix - metabolism</topic><topic>Food Handling - methods</topic><topic>Food Microbiology</topic><topic>Foodborne Diseases - microbiology</topic><topic>Hot Temperature</topic><topic>Microscopy, Confocal</topic><topic>Milk - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ren, Fanchong</creatorcontrib><creatorcontrib>Chen, Yuhang</creatorcontrib><creatorcontrib>Yang, Shuo</creatorcontrib><creatorcontrib>Zhang, Yinan</creatorcontrib><creatorcontrib>Liu, Yangtai</creatorcontrib><creatorcontrib>Ma, Yue</creatorcontrib><creatorcontrib>Wang, Yating</creatorcontrib><creatorcontrib>Liu, Yang</creatorcontrib><creatorcontrib>Dong, Qingli</creatorcontrib><creatorcontrib>Lu, Dasheng</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Food research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ren, Fanchong</au><au>Chen, Yuhang</au><au>Yang, Shuo</au><au>Zhang, Yinan</au><au>Liu, Yangtai</au><au>Ma, Yue</au><au>Wang, Yating</au><au>Liu, Yang</au><au>Dong, Qingli</au><au>Lu, Dasheng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of emetic Bacillus cereus biofilm formation and cereulide production in biofilm</atitle><jtitle>Food research international</jtitle><addtitle>Food Res Int</addtitle><date>2024-09</date><risdate>2024</risdate><volume>192</volume><spage>114834</spage><pages>114834-</pages><artnum>114834</artnum><issn>0963-9969</issn><issn>1873-7145</issn><eissn>1873-7145</eissn><abstract>[Display omitted]
•Dynamic processes of biofilm formation and cereulide production under four different conditions.•The substrate has an impact on the formation of biofilms and cereulide of Bacillus cereus.•The cereulide levels in biofilms formed in BHI medium are mostly higher than those formed in UHT milk.
Bacillus cereus is a well-known foodborne pathogen that can cause human diseases, including vomiting caused by emetic toxin, cereulide, requiring 105-108 cells per gram to cause the disease. The bacterial cells may be eliminated during processing, but cereulide can survive in most processing techniques due to its resistance to high temperatures, extreme pH and proteolytic enzymes. Herein, we reported dynamic processes of biofilm formation of four different types and cereulide production within the biofilm. Confocal laser scanning microscopy (CLSM) images revealed that biofilms of the four different types reach each stage at different time points. Among the extracellular polymeric substances (EPS) components of the four biofilms formed by the emetic B. cereus F4810/72 strain, proteins account for the majority. In addition, there are significant differences (p < 0.05) in the EPS components at the same stage among biofilms of different types. The time point at which cereulide was first detected in the four types of biofilms was 24 h. In the biofilm of B. cereus formed in ultra-high-temperature (UHT) milk, the first peak of cereulide appeared at 72 h. The cereulide content of the biofilms formed in BHI was mostly higher than that of the biofilms formed in UHT milk. This study contributes to a better understanding of food safety issues in the industry caused by biofilm and cereulide toxin produced by B. cereus.</abstract><cop>Canada</cop><pub>Elsevier Ltd</pub><pmid>39147521</pmid><doi>10.1016/j.foodres.2024.114834</doi><orcidid>https://orcid.org/0000-0001-6352-4002</orcidid><orcidid>https://orcid.org/0000-0001-6981-0878</orcidid><orcidid>https://orcid.org/0000-0002-5665-034X</orcidid><orcidid>https://orcid.org/0009-0003-6730-0016</orcidid><orcidid>https://orcid.org/0000-0003-2409-8666</orcidid><orcidid>https://orcid.org/0000-0001-9019-0001</orcidid><orcidid>https://orcid.org/0009-0004-8204-7626</orcidid><orcidid>https://orcid.org/0000-0001-6961-3874</orcidid></addata></record> |
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| subjects | Animals Bacillus cereus Bacillus cereus - metabolism Bacillus cereus - physiology Biofilm Biofilms - growth & development Cereulide CLSM Depsipeptides - metabolism EPS Extracellular Polymeric Substance Matrix - metabolism Food Handling - methods Food Microbiology Foodborne Diseases - microbiology Hot Temperature Microscopy, Confocal Milk - microbiology |
| title | Characterization of emetic Bacillus cereus biofilm formation and cereulide production in biofilm |
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