ARTP mutagenesis of Aureobasidium pullulans RM1603 for high pullulan production and transcriptome analysis of mutants
Pullulan is a microbial exopolysaccharide produced by Aureobasidium spp. with excellent physical and chemical properties, resulting in great application value. In this study, a novel strain RM1603 of Aureobasidium pullulans with high pullulan production of 51.0 ± 1.0 g·L − 1 isolated from rhizospher...
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| Veröffentlicht in: | Archives of microbiology 2024-09, Vol.206 (9), p.375, Article 375 |
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| creator | Bai, Ruoxuan Chen, Jiale Hao, Yaqiao Dong, Yiheng Ren, Keyao Gao, Ting Zhang, Shuting Xu, Fangxu Zhao, Hongxin |
| description | Pullulan is a microbial exopolysaccharide produced by
Aureobasidium
spp. with excellent physical and chemical properties, resulting in great application value. In this study, a novel strain RM1603 of
Aureobasidium pullulans
with high pullulan production of 51.0 ± 1.0 g·L
− 1
isolated from rhizosphere soil was subjected to atmospheric and room temperature plasma (ARTP) mutagenesis, followed by selection of mutants to obtain pullulan high-producing strains. Finally, two mutants Mu0816 and Mu1519 were obtained, with polysaccharide productions of 58.7 ± 0.8 and 60.0 ± 0.8 g∙L
− 1
after 72-h fermentation, representing 15.1 and 17.6% increases compared with the original strain, respectively. Transcriptome analysis of the two mutants and the original strain revealed that the high expression of α/β-hydrolase (ABHD), α-amylase (AMY1), and sugar porter family MFS transporters (SPF-MFS) in the mutants may be related to the synthesis and secretion of pullulan. These results demonstrated the effectiveness of ARTP mutagenesis in
A. pullulans
, providing a basis for the investigation of genes related to pullulan synthesis and secretion.
Graphical Abstract |
| doi_str_mv | 10.1007/s00203-024-04094-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3092872016</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3092950732</sourcerecordid><originalsourceid>FETCH-LOGICAL-c256t-383bd7e3c4169af5eabe3bdef8a1d63611ddf2ce9e7a697c2c7c3d94b1e6f8fa3</originalsourceid><addsrcrecordid>eNp9kU9r3DAQxUVoSLZpvkAORZBLL25GkteWjkvoP0hJCCnkJmRptHGwra1kHfLtq9SbFHLoaWDm9948eIScMfjMANqLBMBBVMDrCmpQdcUOyIrVglfQ8vt3ZAUCeCWVEMfkfUqPAIxLKY_IsVCsZkzIFcmb27sbOubZbHHC1CcaPN3kiKEzqXd9HukuD0MezJTo7U_WgKA-RPrQbx9eL3QXg8t27sNEzeToHAttY7-bw4hlY4anvfPzo2lOH8ihN0PC0_08Ib--frm7_F5dXX_7cbm5qixfN3MlpOhci8LWrFHGr9F0WDbopWGuEQ1jznluUWFrGtVablsrnKo7ho2X3ogT8mnxLQF_Z0yzHvtkcSiZMeSkBSguWw6sKej5G_Qx5FiiL5RaQyt4ofhC2RhSiuj1LvajiU-agX4uRS-l6FKK_luKZkX0cW-duxHdq-SlhQKIBUjlNG0x_vv9H9s_S46ZZw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3092950732</pqid></control><display><type>article</type><title>ARTP mutagenesis of Aureobasidium pullulans RM1603 for high pullulan production and transcriptome analysis of mutants</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Bai, Ruoxuan ; Chen, Jiale ; Hao, Yaqiao ; Dong, Yiheng ; Ren, Keyao ; Gao, Ting ; Zhang, Shuting ; Xu, Fangxu ; Zhao, Hongxin</creator><creatorcontrib>Bai, Ruoxuan ; Chen, Jiale ; Hao, Yaqiao ; Dong, Yiheng ; Ren, Keyao ; Gao, Ting ; Zhang, Shuting ; Xu, Fangxu ; Zhao, Hongxin</creatorcontrib><description>Pullulan is a microbial exopolysaccharide produced by
Aureobasidium
spp. with excellent physical and chemical properties, resulting in great application value. In this study, a novel strain RM1603 of
Aureobasidium pullulans
with high pullulan production of 51.0 ± 1.0 g·L
− 1
isolated from rhizosphere soil was subjected to atmospheric and room temperature plasma (ARTP) mutagenesis, followed by selection of mutants to obtain pullulan high-producing strains. Finally, two mutants Mu0816 and Mu1519 were obtained, with polysaccharide productions of 58.7 ± 0.8 and 60.0 ± 0.8 g∙L
− 1
after 72-h fermentation, representing 15.1 and 17.6% increases compared with the original strain, respectively. Transcriptome analysis of the two mutants and the original strain revealed that the high expression of α/β-hydrolase (ABHD), α-amylase (AMY1), and sugar porter family MFS transporters (SPF-MFS) in the mutants may be related to the synthesis and secretion of pullulan. These results demonstrated the effectiveness of ARTP mutagenesis in
A. pullulans
, providing a basis for the investigation of genes related to pullulan synthesis and secretion.
Graphical Abstract</description><identifier>ISSN: 0302-8933</identifier><identifier>ISSN: 1432-072X</identifier><identifier>EISSN: 1432-072X</identifier><identifier>DOI: 10.1007/s00203-024-04094-1</identifier><identifier>PMID: 39141138</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>alpha-Amylases - genetics ; alpha-Amylases - metabolism ; Aureobasidium - genetics ; Aureobasidium - metabolism ; Aureobasidium pullulans ; Biochemistry ; Biomedical and Life Sciences ; Biotechnology ; Cell Biology ; Chemical properties ; Ecology ; Exopolysaccharides ; Fermentation ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; Fungi ; Gene Expression Profiling ; Glucans - metabolism ; Life Sciences ; Microbial Ecology ; Microbiology ; Microorganisms ; Mutagenesis ; Mutants ; Mutation ; Original Paper ; Polysaccharides ; Pullulan ; Rhizosphere ; Room temperature ; Secretion ; Soil Microbiology ; Soil temperature ; Strain analysis ; Synthesis ; Transcriptome ; Transcriptomes ; α-Amylase</subject><ispartof>Archives of microbiology, 2024-09, Vol.206 (9), p.375, Article 375</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c256t-383bd7e3c4169af5eabe3bdef8a1d63611ddf2ce9e7a697c2c7c3d94b1e6f8fa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00203-024-04094-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00203-024-04094-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27928,27929,41492,42561,51323</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39141138$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bai, Ruoxuan</creatorcontrib><creatorcontrib>Chen, Jiale</creatorcontrib><creatorcontrib>Hao, Yaqiao</creatorcontrib><creatorcontrib>Dong, Yiheng</creatorcontrib><creatorcontrib>Ren, Keyao</creatorcontrib><creatorcontrib>Gao, Ting</creatorcontrib><creatorcontrib>Zhang, Shuting</creatorcontrib><creatorcontrib>Xu, Fangxu</creatorcontrib><creatorcontrib>Zhao, Hongxin</creatorcontrib><title>ARTP mutagenesis of Aureobasidium pullulans RM1603 for high pullulan production and transcriptome analysis of mutants</title><title>Archives of microbiology</title><addtitle>Arch Microbiol</addtitle><addtitle>Arch Microbiol</addtitle><description>Pullulan is a microbial exopolysaccharide produced by
Aureobasidium
spp. with excellent physical and chemical properties, resulting in great application value. In this study, a novel strain RM1603 of
Aureobasidium pullulans
with high pullulan production of 51.0 ± 1.0 g·L
− 1
isolated from rhizosphere soil was subjected to atmospheric and room temperature plasma (ARTP) mutagenesis, followed by selection of mutants to obtain pullulan high-producing strains. Finally, two mutants Mu0816 and Mu1519 were obtained, with polysaccharide productions of 58.7 ± 0.8 and 60.0 ± 0.8 g∙L
− 1
after 72-h fermentation, representing 15.1 and 17.6% increases compared with the original strain, respectively. Transcriptome analysis of the two mutants and the original strain revealed that the high expression of α/β-hydrolase (ABHD), α-amylase (AMY1), and sugar porter family MFS transporters (SPF-MFS) in the mutants may be related to the synthesis and secretion of pullulan. These results demonstrated the effectiveness of ARTP mutagenesis in
A. pullulans
, providing a basis for the investigation of genes related to pullulan synthesis and secretion.
Graphical Abstract</description><subject>alpha-Amylases - genetics</subject><subject>alpha-Amylases - metabolism</subject><subject>Aureobasidium - genetics</subject><subject>Aureobasidium - metabolism</subject><subject>Aureobasidium pullulans</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cell Biology</subject><subject>Chemical properties</subject><subject>Ecology</subject><subject>Exopolysaccharides</subject><subject>Fermentation</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - metabolism</subject><subject>Fungi</subject><subject>Gene Expression Profiling</subject><subject>Glucans - metabolism</subject><subject>Life Sciences</subject><subject>Microbial Ecology</subject><subject>Microbiology</subject><subject>Microorganisms</subject><subject>Mutagenesis</subject><subject>Mutants</subject><subject>Mutation</subject><subject>Original Paper</subject><subject>Polysaccharides</subject><subject>Pullulan</subject><subject>Rhizosphere</subject><subject>Room temperature</subject><subject>Secretion</subject><subject>Soil Microbiology</subject><subject>Soil temperature</subject><subject>Strain analysis</subject><subject>Synthesis</subject><subject>Transcriptome</subject><subject>Transcriptomes</subject><subject>α-Amylase</subject><issn>0302-8933</issn><issn>1432-072X</issn><issn>1432-072X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU9r3DAQxUVoSLZpvkAORZBLL25GkteWjkvoP0hJCCnkJmRptHGwra1kHfLtq9SbFHLoaWDm9948eIScMfjMANqLBMBBVMDrCmpQdcUOyIrVglfQ8vt3ZAUCeCWVEMfkfUqPAIxLKY_IsVCsZkzIFcmb27sbOubZbHHC1CcaPN3kiKEzqXd9HukuD0MezJTo7U_WgKA-RPrQbx9eL3QXg8t27sNEzeToHAttY7-bw4hlY4anvfPzo2lOH8ihN0PC0_08Ib--frm7_F5dXX_7cbm5qixfN3MlpOhci8LWrFHGr9F0WDbopWGuEQ1jznluUWFrGtVablsrnKo7ho2X3ogT8mnxLQF_Z0yzHvtkcSiZMeSkBSguWw6sKej5G_Qx5FiiL5RaQyt4ofhC2RhSiuj1LvajiU-agX4uRS-l6FKK_luKZkX0cW-duxHdq-SlhQKIBUjlNG0x_vv9H9s_S46ZZw</recordid><startdate>20240901</startdate><enddate>20240901</enddate><creator>Bai, Ruoxuan</creator><creator>Chen, Jiale</creator><creator>Hao, Yaqiao</creator><creator>Dong, Yiheng</creator><creator>Ren, Keyao</creator><creator>Gao, Ting</creator><creator>Zhang, Shuting</creator><creator>Xu, Fangxu</creator><creator>Zhao, Hongxin</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20240901</creationdate><title>ARTP mutagenesis of Aureobasidium pullulans RM1603 for high pullulan production and transcriptome analysis of mutants</title><author>Bai, Ruoxuan ; Chen, Jiale ; Hao, Yaqiao ; Dong, Yiheng ; Ren, Keyao ; Gao, Ting ; Zhang, Shuting ; Xu, Fangxu ; Zhao, Hongxin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c256t-383bd7e3c4169af5eabe3bdef8a1d63611ddf2ce9e7a697c2c7c3d94b1e6f8fa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>alpha-Amylases - genetics</topic><topic>alpha-Amylases - metabolism</topic><topic>Aureobasidium - genetics</topic><topic>Aureobasidium - metabolism</topic><topic>Aureobasidium pullulans</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Cell Biology</topic><topic>Chemical properties</topic><topic>Ecology</topic><topic>Exopolysaccharides</topic><topic>Fermentation</topic><topic>Fungal Proteins - genetics</topic><topic>Fungal Proteins - metabolism</topic><topic>Fungi</topic><topic>Gene Expression Profiling</topic><topic>Glucans - metabolism</topic><topic>Life Sciences</topic><topic>Microbial Ecology</topic><topic>Microbiology</topic><topic>Microorganisms</topic><topic>Mutagenesis</topic><topic>Mutants</topic><topic>Mutation</topic><topic>Original Paper</topic><topic>Polysaccharides</topic><topic>Pullulan</topic><topic>Rhizosphere</topic><topic>Room temperature</topic><topic>Secretion</topic><topic>Soil Microbiology</topic><topic>Soil temperature</topic><topic>Strain analysis</topic><topic>Synthesis</topic><topic>Transcriptome</topic><topic>Transcriptomes</topic><topic>α-Amylase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bai, Ruoxuan</creatorcontrib><creatorcontrib>Chen, Jiale</creatorcontrib><creatorcontrib>Hao, Yaqiao</creatorcontrib><creatorcontrib>Dong, Yiheng</creatorcontrib><creatorcontrib>Ren, Keyao</creatorcontrib><creatorcontrib>Gao, Ting</creatorcontrib><creatorcontrib>Zhang, Shuting</creatorcontrib><creatorcontrib>Xu, Fangxu</creatorcontrib><creatorcontrib>Zhao, Hongxin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bai, Ruoxuan</au><au>Chen, Jiale</au><au>Hao, Yaqiao</au><au>Dong, Yiheng</au><au>Ren, Keyao</au><au>Gao, Ting</au><au>Zhang, Shuting</au><au>Xu, Fangxu</au><au>Zhao, Hongxin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>ARTP mutagenesis of Aureobasidium pullulans RM1603 for high pullulan production and transcriptome analysis of mutants</atitle><jtitle>Archives of microbiology</jtitle><stitle>Arch Microbiol</stitle><addtitle>Arch Microbiol</addtitle><date>2024-09-01</date><risdate>2024</risdate><volume>206</volume><issue>9</issue><spage>375</spage><pages>375-</pages><artnum>375</artnum><issn>0302-8933</issn><issn>1432-072X</issn><eissn>1432-072X</eissn><abstract>Pullulan is a microbial exopolysaccharide produced by
Aureobasidium
spp. with excellent physical and chemical properties, resulting in great application value. In this study, a novel strain RM1603 of
Aureobasidium pullulans
with high pullulan production of 51.0 ± 1.0 g·L
− 1
isolated from rhizosphere soil was subjected to atmospheric and room temperature plasma (ARTP) mutagenesis, followed by selection of mutants to obtain pullulan high-producing strains. Finally, two mutants Mu0816 and Mu1519 were obtained, with polysaccharide productions of 58.7 ± 0.8 and 60.0 ± 0.8 g∙L
− 1
after 72-h fermentation, representing 15.1 and 17.6% increases compared with the original strain, respectively. Transcriptome analysis of the two mutants and the original strain revealed that the high expression of α/β-hydrolase (ABHD), α-amylase (AMY1), and sugar porter family MFS transporters (SPF-MFS) in the mutants may be related to the synthesis and secretion of pullulan. These results demonstrated the effectiveness of ARTP mutagenesis in
A. pullulans
, providing a basis for the investigation of genes related to pullulan synthesis and secretion.
Graphical Abstract</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>39141138</pmid><doi>10.1007/s00203-024-04094-1</doi></addata></record> |
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| subjects | alpha-Amylases - genetics alpha-Amylases - metabolism Aureobasidium - genetics Aureobasidium - metabolism Aureobasidium pullulans Biochemistry Biomedical and Life Sciences Biotechnology Cell Biology Chemical properties Ecology Exopolysaccharides Fermentation Fungal Proteins - genetics Fungal Proteins - metabolism Fungi Gene Expression Profiling Glucans - metabolism Life Sciences Microbial Ecology Microbiology Microorganisms Mutagenesis Mutants Mutation Original Paper Polysaccharides Pullulan Rhizosphere Room temperature Secretion Soil Microbiology Soil temperature Strain analysis Synthesis Transcriptome Transcriptomes α-Amylase |
| title | ARTP mutagenesis of Aureobasidium pullulans RM1603 for high pullulan production and transcriptome analysis of mutants |
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