Genome editing-based mutagenesis stably modifies composition of wax esters synthesized by Euglena gracilis under anaerobic conditions

[Display omitted] •Stably altered wax ester composition in Euglena gracilis using CRISPR/Cas9.•Knocked out beta-oxidation enzymes, enabling controlled wax ester synthesis.•Developed mutants with potential for diverse chemical and fuel applications.•Demonstrated additive effects in mutations that sho...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Bioresource technology 2024-10, Vol.410, p.131255, Article 131255
Hauptverfasser:	Nagamine, Sakura, Oishi, Rikuto, Ueda, Mitsuhiro, Sakamoto, Tatsuji, Nakazawa, Masami
Format:	Artikel
Sprache:	eng
Schlagworte:	3-Ketoacyl-CoA thiolase acyl coenzyme A Acyl-CoA dehydrogenase Anaerobiosis beta oxidation Biofuel biofuels biorefining Esters - chemistry Esters - metabolism Euglena gracilis Euglena gracilis - genetics Euglena gracilis - metabolism fatty acids Fatty Acids - metabolism feedstocks Gene Editing - methods genome Metabolic engineering Metabolic Engineering - methods microalgae Mutagenesis mutants Mutation - genetics oils Waxes - metabolism β-Oxidation reversal
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!