Calcium-Dependent Protein Kinase GhCDPK16 Exerts a Positive Regulatory Role in Enhancing Drought Tolerance in Cotton
Cotton is essential for the textile industry as a primary source of natural fibers. However, environmental factors like drought present significant challenges to its cultivation, adversely affecting both production levels and fiber quality. Enhancing cotton's drought resilience has the potentia...
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| Veröffentlicht in: | International journal of molecular sciences 2024-08, Vol.25 (15), p.8308 |
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| creator | Yan, Mengyuan Chai, Meijie Li, Libei Dong, Zhiwei Jin, Hongmiao Tan, Ming Ye, Ziwei Yu, Shuxun Feng, Zhen |
| description | Cotton is essential for the textile industry as a primary source of natural fibers. However, environmental factors like drought present significant challenges to its cultivation, adversely affecting both production levels and fiber quality. Enhancing cotton's drought resilience has the potential to reduce yield losses and support the growth of cotton farming. In this study, the cotton calcium-dependent protein kinase GhCDPK16 was characterized, and the transcription level of
was significantly upregulated under drought and various stress-related hormone treatments. Physiological analyses revealed that the overexpression of
improved drought stress resistance in Arabidopsis by enhancing osmotic adjustment capacity and boosting antioxidant enzyme activities. In contrast, silencing
in cotton resulted in increased dehydration compared with the control. Furthermore, reduced antioxidant enzyme activities and downregulation of ABA-related genes were observed in
-silenced plants. These findings not only enhanced our understanding of the biological functions of
and the mechanisms underlying drought stress resistance but also underscored the considerable potential of
in improving drought resilience in cotton. |
| doi_str_mv | 10.3390/ijms25158308 |
| format | Article |
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was significantly upregulated under drought and various stress-related hormone treatments. Physiological analyses revealed that the overexpression of
improved drought stress resistance in Arabidopsis by enhancing osmotic adjustment capacity and boosting antioxidant enzyme activities. In contrast, silencing
in cotton resulted in increased dehydration compared with the control. Furthermore, reduced antioxidant enzyme activities and downregulation of ABA-related genes were observed in
-silenced plants. These findings not only enhanced our understanding of the biological functions of
and the mechanisms underlying drought stress resistance but also underscored the considerable potential of
in improving drought resilience in cotton.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms25158308</identifier><identifier>PMID: 39125876</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Abiotic stress ; Abscisic acid ; Amino acids ; Arabidopsis - genetics ; Arabidopsis - physiology ; Cotton ; Cotton industry ; Cytoplasm ; Drought ; Drought Resistance - genetics ; Environmental conditions ; Gene Expression Regulation, Plant ; Genes ; Gossypium - genetics ; Gossypium - metabolism ; Gossypium - physiology ; Hormones ; Kinases ; Localization ; Phosphorylation ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant resistance ; Plants, Genetically Modified ; Protein kinases ; Protein Kinases - genetics ; Protein Kinases - metabolism ; Proteins ; Stress, Physiological</subject><ispartof>International journal of molecular sciences, 2024-08, Vol.25 (15), p.8308</ispartof><rights>COPYRIGHT 2024 MDPI AG</rights><rights>2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c283t-1ce920f20b84a90370a2381344bbfaefea0030afd9715a60b6e0b583048dbb613</cites><orcidid>0000-0003-3338-1025 ; 0000-0003-3667-603X ; 0000-0003-1138-134X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39125876$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yan, Mengyuan</creatorcontrib><creatorcontrib>Chai, Meijie</creatorcontrib><creatorcontrib>Li, Libei</creatorcontrib><creatorcontrib>Dong, Zhiwei</creatorcontrib><creatorcontrib>Jin, Hongmiao</creatorcontrib><creatorcontrib>Tan, Ming</creatorcontrib><creatorcontrib>Ye, Ziwei</creatorcontrib><creatorcontrib>Yu, Shuxun</creatorcontrib><creatorcontrib>Feng, Zhen</creatorcontrib><title>Calcium-Dependent Protein Kinase GhCDPK16 Exerts a Positive Regulatory Role in Enhancing Drought Tolerance in Cotton</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>Cotton is essential for the textile industry as a primary source of natural fibers. However, environmental factors like drought present significant challenges to its cultivation, adversely affecting both production levels and fiber quality. Enhancing cotton's drought resilience has the potential to reduce yield losses and support the growth of cotton farming. In this study, the cotton calcium-dependent protein kinase GhCDPK16 was characterized, and the transcription level of
was significantly upregulated under drought and various stress-related hormone treatments. Physiological analyses revealed that the overexpression of
improved drought stress resistance in Arabidopsis by enhancing osmotic adjustment capacity and boosting antioxidant enzyme activities. In contrast, silencing
in cotton resulted in increased dehydration compared with the control. Furthermore, reduced antioxidant enzyme activities and downregulation of ABA-related genes were observed in
-silenced plants. These findings not only enhanced our understanding of the biological functions of
and the mechanisms underlying drought stress resistance but also underscored the considerable potential of
in improving drought resilience in cotton.</description><subject>Abiotic stress</subject><subject>Abscisic acid</subject><subject>Amino acids</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - physiology</subject><subject>Cotton</subject><subject>Cotton industry</subject><subject>Cytoplasm</subject><subject>Drought</subject><subject>Drought Resistance - genetics</subject><subject>Environmental conditions</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes</subject><subject>Gossypium - genetics</subject><subject>Gossypium - metabolism</subject><subject>Gossypium - physiology</subject><subject>Hormones</subject><subject>Kinases</subject><subject>Localization</subject><subject>Phosphorylation</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plant resistance</subject><subject>Plants, Genetically Modified</subject><subject>Protein kinases</subject><subject>Protein Kinases - genetics</subject><subject>Protein Kinases - metabolism</subject><subject>Proteins</subject><subject>Stress, Physiological</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkUFr3DAQhUVpadJtbz0XQS89xOlIsmz5GLybNCTQJaRnI9vjXS22tJXkkvz7aLtpCWFAI2a-GR7zCPnM4FyICr6b3RS4ZFIJUG_IKcs5zwCK8u2L_wn5EMIOgAsuq_fkRFSMS1UWpyTWeuzMPGVL3KPt0Ua69i6isfTGWB2QXm3r5fqGFXT1gD4GqunaBRPNH6R3uJlHHZ1_pHduRJqGVnarbWfshi69mzfbSO9Tx6fa33btYnT2I3k36DHgp-e8IL8uV_f1j-z259V1fXGbdVyJmLEOKw4Dh1blugJRguZCMZHnbTtoHFADCNBDX5VM6gLaAqE93CFXfdsWTCzIt-PevXe_ZwyxmUzocBy1RTeHRkC6gyqhUAn9-grdudnbpO5AQSWkSs-CnB-pjR6xMXZw0esuRY-T6ZzFwaT6hYJcMiblQcHZcaDzLgSPQ7P3ZtL-sWHQHOxrXtqX8C_PKuZ2wv4__M8v8QQA-ZRk</recordid><startdate>20240801</startdate><enddate>20240801</enddate><creator>Yan, Mengyuan</creator><creator>Chai, Meijie</creator><creator>Li, Libei</creator><creator>Dong, Zhiwei</creator><creator>Jin, Hongmiao</creator><creator>Tan, Ming</creator><creator>Ye, Ziwei</creator><creator>Yu, Shuxun</creator><creator>Feng, Zhen</creator><general>MDPI AG</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-3338-1025</orcidid><orcidid>https://orcid.org/0000-0003-3667-603X</orcidid><orcidid>https://orcid.org/0000-0003-1138-134X</orcidid></search><sort><creationdate>20240801</creationdate><title>Calcium-Dependent Protein Kinase GhCDPK16 Exerts a Positive Regulatory Role in Enhancing Drought Tolerance in Cotton</title><author>Yan, Mengyuan ; 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However, environmental factors like drought present significant challenges to its cultivation, adversely affecting both production levels and fiber quality. Enhancing cotton's drought resilience has the potential to reduce yield losses and support the growth of cotton farming. In this study, the cotton calcium-dependent protein kinase GhCDPK16 was characterized, and the transcription level of
was significantly upregulated under drought and various stress-related hormone treatments. Physiological analyses revealed that the overexpression of
improved drought stress resistance in Arabidopsis by enhancing osmotic adjustment capacity and boosting antioxidant enzyme activities. In contrast, silencing
in cotton resulted in increased dehydration compared with the control. Furthermore, reduced antioxidant enzyme activities and downregulation of ABA-related genes were observed in
-silenced plants. These findings not only enhanced our understanding of the biological functions of
and the mechanisms underlying drought stress resistance but also underscored the considerable potential of
in improving drought resilience in cotton.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>39125876</pmid><doi>10.3390/ijms25158308</doi><orcidid>https://orcid.org/0000-0003-3338-1025</orcidid><orcidid>https://orcid.org/0000-0003-3667-603X</orcidid><orcidid>https://orcid.org/0000-0003-1138-134X</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Abiotic stress Abscisic acid Amino acids Arabidopsis - genetics Arabidopsis - physiology Cotton Cotton industry Cytoplasm Drought Drought Resistance - genetics Environmental conditions Gene Expression Regulation, Plant Genes Gossypium - genetics Gossypium - metabolism Gossypium - physiology Hormones Kinases Localization Phosphorylation Plant Proteins - genetics Plant Proteins - metabolism Plant resistance Plants, Genetically Modified Protein kinases Protein Kinases - genetics Protein Kinases - metabolism Proteins Stress, Physiological |
| title | Calcium-Dependent Protein Kinase GhCDPK16 Exerts a Positive Regulatory Role in Enhancing Drought Tolerance in Cotton |
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