Exploring the interactive impacts of citronellol, thymol, and trans-cinnamaldehyde in broilers: moving toward an improved performance, immunity, gastrointestinal integrity, and Clostridium perfringens resistance
This study aimed to explore the effectiveness of dietary citronellol, thymol, and trans-cinnamaldehyde (CTC) essential oils blend on broilers' growth performance, immunity, intestinal microbial count, gut integrity, and resistance against Clostridium perfringens utilizing the necrotic enteritis...
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| creator | El-Hamid, Marwa I Abd El-Malt, Rania M S Al-Khalaifah, H S Al-Nasser, Afaf Elazab, Sara T Basiony, Asmaa Ali, Amira M Mohamed, Dalia I Nassan, Mohamed A Ibrahim, Doaa |
| description | This study aimed to explore the effectiveness of dietary citronellol, thymol, and trans-cinnamaldehyde (CTC) essential oils blend on broilers' growth performance, immunity, intestinal microbial count, gut integrity, and resistance against Clostridium perfringens utilizing the necrotic enteritis (NE) challenge model.AIMSThis study aimed to explore the effectiveness of dietary citronellol, thymol, and trans-cinnamaldehyde (CTC) essential oils blend on broilers' growth performance, immunity, intestinal microbial count, gut integrity, and resistance against Clostridium perfringens utilizing the necrotic enteritis (NE) challenge model.A total of 200 Ross 308 male broiler chicks received either a control diet or diet supplemented with three graded levels of CTC blend, including 300, 600, and 900 mg of CTC blend/kg diet and experimentally infected with C. perfringens strain at 23 days of age. Herein, dietary CTC blend fortifications significantly improved the broilers' growth performance, which was supported by upregulating the expression levels of MUC-2, occludin, and JAM-2 genes. Moreover, dietary CTC blend inclusion significantly enhanced the levels of blood phagocytic percentage and serum IgA, IgG, and MPO, and reduced the values of serum CRP, and NO at 5 days pre-infection, 10-, and 15 days post-infection (dpi) with C. perfringens. At 15 dpi, CTC blend inclusion significantly reduced the intestinal digesta pH, coliforms and C. perfringens loads, and the expression levels of genes related to C. perfringens virulence (cpe, cnaA, and nanI), proinflammatory cytokines (IL-1β and TNF-α), and chemokines (CCL20), in addition to increasing the count of beneficial total Lactobacillus and total aerobic bacteria, and the expression levels of genes related to anti-inflammatory cytokines (IL-10) and chemokines (AvBD6 and AvBD612).METHODS AND RESULTSA total of 200 Ross 308 male broiler chicks received either a control diet or diet supplemented with three graded levels of CTC blend, including 300, 600, and 900 mg of CTC blend/kg diet and experimentally infected with C. perfringens strain at 23 days of age. Herein, dietary CTC blend fortifications significantly improved the broilers' growth performance, which was supported by upregulating the expression levels of MUC-2, occludin, and JAM-2 genes. Moreover, dietary CTC blend inclusion significantly enhanced the levels of blood phagocytic percentage and serum IgA, IgG, and MPO, and reduced the values of serum CRP, and NO at 5 |
| doi_str_mv | 10.1093/jambio/lxae206 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest</sourceid><recordid>TN_cdi_proquest_miscellaneous_3091283484</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3091283484</sourcerecordid><originalsourceid>FETCH-LOGICAL-p118t-ed271a922e553d905190664c7488de9ec8d7b13fd7da55848fb5044cd2bf7f953</originalsourceid><addsrcrecordid>eNpNkMlOwzAQhiMEEqVw5ewjh4bacZyFG6rKIlXiAufKiSetKy_Fdkr7nLwQTuDAaX7N8s0_kyS3BN8TXNP5jutG2rk6cshwcZZMCC1YmhVldv5PXyZX3u8wJhSzYpJ8L497ZZ00GxS2gKQJ4Hgb5CFqvY_KI9uhVgZnDShl1Sz2nfQQuREoOG582kpjuOZKwPYkBghqnJUKnH9A2h5GuP3iTsSZAevsAQTag-us09y0MItZ3RsZTjO04T4uG4z4IA1Xo6eNG2vDyoWysUEK2esRMXgH45EDL30YaNfJRceVh5u_OE0-npbvi5d09fb8unhcpXtCqpCCyErC6ywDxqioMSM1Loq8LfOqElBDW4myIbQTpeCMVXnVNQzneSuypiu7mtFpcvfLjQd99tHuWkvfxi9xA7b3a4prklU0r3L6A-t3h80</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3091283484</pqid></control><display><type>article</type><title>Exploring the interactive impacts of citronellol, thymol, and trans-cinnamaldehyde in broilers: moving toward an improved performance, immunity, gastrointestinal integrity, and Clostridium perfringens resistance</title><source>Oxford University Press Journals All Titles (1996-Current)</source><creator>El-Hamid, Marwa I Abd ; El-Malt, Rania M S ; Al-Khalaifah, H S ; Al-Nasser, Afaf ; Elazab, Sara T ; Basiony, Asmaa ; Ali, Amira M ; Mohamed, Dalia I ; Nassan, Mohamed A ; Ibrahim, Doaa</creator><creatorcontrib>El-Hamid, Marwa I Abd ; El-Malt, Rania M S ; Al-Khalaifah, H S ; Al-Nasser, Afaf ; Elazab, Sara T ; Basiony, Asmaa ; Ali, Amira M ; Mohamed, Dalia I ; Nassan, Mohamed A ; Ibrahim, Doaa</creatorcontrib><description>This study aimed to explore the effectiveness of dietary citronellol, thymol, and trans-cinnamaldehyde (CTC) essential oils blend on broilers' growth performance, immunity, intestinal microbial count, gut integrity, and resistance against Clostridium perfringens utilizing the necrotic enteritis (NE) challenge model.AIMSThis study aimed to explore the effectiveness of dietary citronellol, thymol, and trans-cinnamaldehyde (CTC) essential oils blend on broilers' growth performance, immunity, intestinal microbial count, gut integrity, and resistance against Clostridium perfringens utilizing the necrotic enteritis (NE) challenge model.A total of 200 Ross 308 male broiler chicks received either a control diet or diet supplemented with three graded levels of CTC blend, including 300, 600, and 900 mg of CTC blend/kg diet and experimentally infected with C. perfringens strain at 23 days of age. Herein, dietary CTC blend fortifications significantly improved the broilers' growth performance, which was supported by upregulating the expression levels of MUC-2, occludin, and JAM-2 genes. Moreover, dietary CTC blend inclusion significantly enhanced the levels of blood phagocytic percentage and serum IgA, IgG, and MPO, and reduced the values of serum CRP, and NO at 5 days pre-infection, 10-, and 15 days post-infection (dpi) with C. perfringens. At 15 dpi, CTC blend inclusion significantly reduced the intestinal digesta pH, coliforms and C. perfringens loads, and the expression levels of genes related to C. perfringens virulence (cpe, cnaA, and nanI), proinflammatory cytokines (IL-1β and TNF-α), and chemokines (CCL20), in addition to increasing the count of beneficial total Lactobacillus and total aerobic bacteria, and the expression levels of genes related to anti-inflammatory cytokines (IL-10) and chemokines (AvBD6 and AvBD612).METHODS AND RESULTSA total of 200 Ross 308 male broiler chicks received either a control diet or diet supplemented with three graded levels of CTC blend, including 300, 600, and 900 mg of CTC blend/kg diet and experimentally infected with C. perfringens strain at 23 days of age. Herein, dietary CTC blend fortifications significantly improved the broilers' growth performance, which was supported by upregulating the expression levels of MUC-2, occludin, and JAM-2 genes. Moreover, dietary CTC blend inclusion significantly enhanced the levels of blood phagocytic percentage and serum IgA, IgG, and MPO, and reduced the values of serum CRP, and NO at 5 days pre-infection, 10-, and 15 days post-infection (dpi) with C. perfringens. At 15 dpi, CTC blend inclusion significantly reduced the intestinal digesta pH, coliforms and C. perfringens loads, and the expression levels of genes related to C. perfringens virulence (cpe, cnaA, and nanI), proinflammatory cytokines (IL-1β and TNF-α), and chemokines (CCL20), in addition to increasing the count of beneficial total Lactobacillus and total aerobic bacteria, and the expression levels of genes related to anti-inflammatory cytokines (IL-10) and chemokines (AvBD6 and AvBD612).Our results point to the growth-provoking, immunostimulant, antibacterial, anti-inflammatory, and antivirulence characteristics of the CTC blend, which improves the broilers' resistance to C. perfringens and ameliorates the negative impacts of NE.CONCLUSIONOur results point to the growth-provoking, immunostimulant, antibacterial, anti-inflammatory, and antivirulence characteristics of the CTC blend, which improves the broilers' resistance to C. perfringens and ameliorates the negative impacts of NE.</description><identifier>ISSN: 1365-2672</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1093/jambio/lxae206</identifier><language>eng</language><ispartof>Journal of applied microbiology, 2024-10, Vol.135 (10)</ispartof><rights>The Author(s) 2024. Published by Oxford University Press on behalf of Applied Microbiology International.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>El-Hamid, Marwa I Abd</creatorcontrib><creatorcontrib>El-Malt, Rania M S</creatorcontrib><creatorcontrib>Al-Khalaifah, H S</creatorcontrib><creatorcontrib>Al-Nasser, Afaf</creatorcontrib><creatorcontrib>Elazab, Sara T</creatorcontrib><creatorcontrib>Basiony, Asmaa</creatorcontrib><creatorcontrib>Ali, Amira M</creatorcontrib><creatorcontrib>Mohamed, Dalia I</creatorcontrib><creatorcontrib>Nassan, Mohamed A</creatorcontrib><creatorcontrib>Ibrahim, Doaa</creatorcontrib><title>Exploring the interactive impacts of citronellol, thymol, and trans-cinnamaldehyde in broilers: moving toward an improved performance, immunity, gastrointestinal integrity, and Clostridium perfringens resistance</title><title>Journal of applied microbiology</title><description>This study aimed to explore the effectiveness of dietary citronellol, thymol, and trans-cinnamaldehyde (CTC) essential oils blend on broilers' growth performance, immunity, intestinal microbial count, gut integrity, and resistance against Clostridium perfringens utilizing the necrotic enteritis (NE) challenge model.AIMSThis study aimed to explore the effectiveness of dietary citronellol, thymol, and trans-cinnamaldehyde (CTC) essential oils blend on broilers' growth performance, immunity, intestinal microbial count, gut integrity, and resistance against Clostridium perfringens utilizing the necrotic enteritis (NE) challenge model.A total of 200 Ross 308 male broiler chicks received either a control diet or diet supplemented with three graded levels of CTC blend, including 300, 600, and 900 mg of CTC blend/kg diet and experimentally infected with C. perfringens strain at 23 days of age. Herein, dietary CTC blend fortifications significantly improved the broilers' growth performance, which was supported by upregulating the expression levels of MUC-2, occludin, and JAM-2 genes. Moreover, dietary CTC blend inclusion significantly enhanced the levels of blood phagocytic percentage and serum IgA, IgG, and MPO, and reduced the values of serum CRP, and NO at 5 days pre-infection, 10-, and 15 days post-infection (dpi) with C. perfringens. At 15 dpi, CTC blend inclusion significantly reduced the intestinal digesta pH, coliforms and C. perfringens loads, and the expression levels of genes related to C. perfringens virulence (cpe, cnaA, and nanI), proinflammatory cytokines (IL-1β and TNF-α), and chemokines (CCL20), in addition to increasing the count of beneficial total Lactobacillus and total aerobic bacteria, and the expression levels of genes related to anti-inflammatory cytokines (IL-10) and chemokines (AvBD6 and AvBD612).METHODS AND RESULTSA total of 200 Ross 308 male broiler chicks received either a control diet or diet supplemented with three graded levels of CTC blend, including 300, 600, and 900 mg of CTC blend/kg diet and experimentally infected with C. perfringens strain at 23 days of age. Herein, dietary CTC blend fortifications significantly improved the broilers' growth performance, which was supported by upregulating the expression levels of MUC-2, occludin, and JAM-2 genes. Moreover, dietary CTC blend inclusion significantly enhanced the levels of blood phagocytic percentage and serum IgA, IgG, and MPO, and reduced the values of serum CRP, and NO at 5 days pre-infection, 10-, and 15 days post-infection (dpi) with C. perfringens. At 15 dpi, CTC blend inclusion significantly reduced the intestinal digesta pH, coliforms and C. perfringens loads, and the expression levels of genes related to C. perfringens virulence (cpe, cnaA, and nanI), proinflammatory cytokines (IL-1β and TNF-α), and chemokines (CCL20), in addition to increasing the count of beneficial total Lactobacillus and total aerobic bacteria, and the expression levels of genes related to anti-inflammatory cytokines (IL-10) and chemokines (AvBD6 and AvBD612).Our results point to the growth-provoking, immunostimulant, antibacterial, anti-inflammatory, and antivirulence characteristics of the CTC blend, which improves the broilers' resistance to C. perfringens and ameliorates the negative impacts of NE.CONCLUSIONOur results point to the growth-provoking, immunostimulant, antibacterial, anti-inflammatory, and antivirulence characteristics of the CTC blend, which improves the broilers' resistance to C. perfringens and ameliorates the negative impacts of NE.</description><issn>1365-2672</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNpNkMlOwzAQhiMEEqVw5ewjh4bacZyFG6rKIlXiAufKiSetKy_Fdkr7nLwQTuDAaX7N8s0_kyS3BN8TXNP5jutG2rk6cshwcZZMCC1YmhVldv5PXyZX3u8wJhSzYpJ8L497ZZ00GxS2gKQJ4Hgb5CFqvY_KI9uhVgZnDShl1Sz2nfQQuREoOG582kpjuOZKwPYkBghqnJUKnH9A2h5GuP3iTsSZAevsAQTag-us09y0MItZ3RsZTjO04T4uG4z4IA1Xo6eNG2vDyoWysUEK2esRMXgH45EDL30YaNfJRceVh5u_OE0-npbvi5d09fb8unhcpXtCqpCCyErC6ywDxqioMSM1Loq8LfOqElBDW4myIbQTpeCMVXnVNQzneSuypiu7mtFpcvfLjQd99tHuWkvfxi9xA7b3a4prklU0r3L6A-t3h80</recordid><startdate>20241016</startdate><enddate>20241016</enddate><creator>El-Hamid, Marwa I Abd</creator><creator>El-Malt, Rania M S</creator><creator>Al-Khalaifah, H S</creator><creator>Al-Nasser, Afaf</creator><creator>Elazab, Sara T</creator><creator>Basiony, Asmaa</creator><creator>Ali, Amira M</creator><creator>Mohamed, Dalia I</creator><creator>Nassan, Mohamed A</creator><creator>Ibrahim, Doaa</creator><scope>7X8</scope></search><sort><creationdate>20241016</creationdate><title>Exploring the interactive impacts of citronellol, thymol, and trans-cinnamaldehyde in broilers: moving toward an improved performance, immunity, gastrointestinal integrity, and Clostridium perfringens resistance</title><author>El-Hamid, Marwa I Abd ; El-Malt, Rania M S ; Al-Khalaifah, H S ; Al-Nasser, Afaf ; Elazab, Sara T ; Basiony, Asmaa ; Ali, Amira M ; Mohamed, Dalia I ; Nassan, Mohamed A ; Ibrahim, Doaa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p118t-ed271a922e553d905190664c7488de9ec8d7b13fd7da55848fb5044cd2bf7f953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>El-Hamid, Marwa I Abd</creatorcontrib><creatorcontrib>El-Malt, Rania M S</creatorcontrib><creatorcontrib>Al-Khalaifah, H S</creatorcontrib><creatorcontrib>Al-Nasser, Afaf</creatorcontrib><creatorcontrib>Elazab, Sara T</creatorcontrib><creatorcontrib>Basiony, Asmaa</creatorcontrib><creatorcontrib>Ali, Amira M</creatorcontrib><creatorcontrib>Mohamed, Dalia I</creatorcontrib><creatorcontrib>Nassan, Mohamed A</creatorcontrib><creatorcontrib>Ibrahim, Doaa</creatorcontrib><collection>MEDLINE - Academic</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>El-Hamid, Marwa I Abd</au><au>El-Malt, Rania M S</au><au>Al-Khalaifah, H S</au><au>Al-Nasser, Afaf</au><au>Elazab, Sara T</au><au>Basiony, Asmaa</au><au>Ali, Amira M</au><au>Mohamed, Dalia I</au><au>Nassan, Mohamed A</au><au>Ibrahim, Doaa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Exploring the interactive impacts of citronellol, thymol, and trans-cinnamaldehyde in broilers: moving toward an improved performance, immunity, gastrointestinal integrity, and Clostridium perfringens resistance</atitle><jtitle>Journal of applied microbiology</jtitle><date>2024-10-16</date><risdate>2024</risdate><volume>135</volume><issue>10</issue><issn>1365-2672</issn><eissn>1365-2672</eissn><abstract>This study aimed to explore the effectiveness of dietary citronellol, thymol, and trans-cinnamaldehyde (CTC) essential oils blend on broilers' growth performance, immunity, intestinal microbial count, gut integrity, and resistance against Clostridium perfringens utilizing the necrotic enteritis (NE) challenge model.AIMSThis study aimed to explore the effectiveness of dietary citronellol, thymol, and trans-cinnamaldehyde (CTC) essential oils blend on broilers' growth performance, immunity, intestinal microbial count, gut integrity, and resistance against Clostridium perfringens utilizing the necrotic enteritis (NE) challenge model.A total of 200 Ross 308 male broiler chicks received either a control diet or diet supplemented with three graded levels of CTC blend, including 300, 600, and 900 mg of CTC blend/kg diet and experimentally infected with C. perfringens strain at 23 days of age. Herein, dietary CTC blend fortifications significantly improved the broilers' growth performance, which was supported by upregulating the expression levels of MUC-2, occludin, and JAM-2 genes. Moreover, dietary CTC blend inclusion significantly enhanced the levels of blood phagocytic percentage and serum IgA, IgG, and MPO, and reduced the values of serum CRP, and NO at 5 days pre-infection, 10-, and 15 days post-infection (dpi) with C. perfringens. At 15 dpi, CTC blend inclusion significantly reduced the intestinal digesta pH, coliforms and C. perfringens loads, and the expression levels of genes related to C. perfringens virulence (cpe, cnaA, and nanI), proinflammatory cytokines (IL-1β and TNF-α), and chemokines (CCL20), in addition to increasing the count of beneficial total Lactobacillus and total aerobic bacteria, and the expression levels of genes related to anti-inflammatory cytokines (IL-10) and chemokines (AvBD6 and AvBD612).METHODS AND RESULTSA total of 200 Ross 308 male broiler chicks received either a control diet or diet supplemented with three graded levels of CTC blend, including 300, 600, and 900 mg of CTC blend/kg diet and experimentally infected with C. perfringens strain at 23 days of age. Herein, dietary CTC blend fortifications significantly improved the broilers' growth performance, which was supported by upregulating the expression levels of MUC-2, occludin, and JAM-2 genes. Moreover, dietary CTC blend inclusion significantly enhanced the levels of blood phagocytic percentage and serum IgA, IgG, and MPO, and reduced the values of serum CRP, and NO at 5 days pre-infection, 10-, and 15 days post-infection (dpi) with C. perfringens. At 15 dpi, CTC blend inclusion significantly reduced the intestinal digesta pH, coliforms and C. perfringens loads, and the expression levels of genes related to C. perfringens virulence (cpe, cnaA, and nanI), proinflammatory cytokines (IL-1β and TNF-α), and chemokines (CCL20), in addition to increasing the count of beneficial total Lactobacillus and total aerobic bacteria, and the expression levels of genes related to anti-inflammatory cytokines (IL-10) and chemokines (AvBD6 and AvBD612).Our results point to the growth-provoking, immunostimulant, antibacterial, anti-inflammatory, and antivirulence characteristics of the CTC blend, which improves the broilers' resistance to C. perfringens and ameliorates the negative impacts of NE.CONCLUSIONOur results point to the growth-provoking, immunostimulant, antibacterial, anti-inflammatory, and antivirulence characteristics of the CTC blend, which improves the broilers' resistance to C. perfringens and ameliorates the negative impacts of NE.</abstract><doi>10.1093/jambio/lxae206</doi></addata></record> |
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| title | Exploring the interactive impacts of citronellol, thymol, and trans-cinnamaldehyde in broilers: moving toward an improved performance, immunity, gastrointestinal integrity, and Clostridium perfringens resistance |
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