Tools and methods for cell ablation and cell inhibition in Caenorhabditis elegans

Tools and methods for cell ablation and cell inhibition in Caenorhabditis elegans

To understand the function of cells such as neurons within an organism, it can be instrumental to inhibit cellular function, or to remove the cell (type) from the organism, and thus to observe the consequences on organismic and/or circuit function and animal behavior. A range of approaches and tools...

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Veröffentlicht in:Genetics (Austin) 2024-08
Hauptverfasser: Rentsch, Dennis, Bergs, Amelie, Shao, Jiajie, Elvers, Nora, Ruse, Christiane, Seidenthal, Marius, Aoki, Ichiro, Gottschalk, Alexander
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container_title Genetics (Austin)
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creator Rentsch, Dennis
Bergs, Amelie
Shao, Jiajie
Elvers, Nora
Ruse, Christiane
Seidenthal, Marius
Aoki, Ichiro
Gottschalk, Alexander
description To understand the function of cells such as neurons within an organism, it can be instrumental to inhibit cellular function, or to remove the cell (type) from the organism, and thus to observe the consequences on organismic and/or circuit function and animal behavior. A range of approaches and tools were developed and used over the past few decades that act either constitutively or acutely and reversibly, in systemic or local fashion. These approaches make use of either drugs or genetically encoded tools. Also, there are acutely acting inhibitory tools that require an exogenous trigger like light. Here, we give an overview of such methods developed and used in the nematode Caenorhabditis elegans.
doi_str_mv 10.1093/genetics/iyae119
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source Oxford University Press Journals All Titles (1996-Current)
title Tools and methods for cell ablation and cell inhibition in Caenorhabditis elegans
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