A non-enzymatic, isothermal amplification sensor for quantifying the relative abundance of Akkermansia muciniphila
Herein, we have developed a non-enzymatic, isothermal amplification assay (NIA sensor) based on a catalytic hairpin assembly (CHA) reaction for quantifying the relative abundance of . Through detection of the MUC-1437 gene (limit of detection: 8.3 fM) in a dynamic range from 10 fM to 1 nM, the NIA s...
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| Veröffentlicht in: | Chemical communications (Cambridge, England) England), 2024-08, Vol.60 (68), p.9089-9092 |
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| container_title | Chemical communications (Cambridge, England) |
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| creator | Liu, Bing Shi, Chen Wang, Fan Xu, Fangling Chao, Jie Zhu, Jiapeng Yang, Dongliang Ouyang, Xiangyuan |
| description | Herein, we have developed a non-enzymatic, isothermal amplification assay (NIA sensor) based on a catalytic hairpin assembly (CHA) reaction for quantifying the relative abundance of
. Through detection of the MUC-1437 gene (limit of detection: 8.3 fM) in a dynamic range from 10 fM to 1 nM, the NIA sensor shows high sensitivity and selectivity in preclinical models of mice fed a normal or high-fat diet (HFD), and treated with antibiotics (ATB). The NIA sensor, which operates without the use of any enzymes, leading to simplicity and cost-effectiveness, has great potential for biosensing research and clinical diagnostic applications. |
| doi_str_mv | 10.1039/d4cc03087g |
| format | Article |
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The NIA sensor, which operates without the use of any enzymes, leading to simplicity and cost-effectiveness, has great potential for biosensing research and clinical diagnostic applications.</description><subject>Akkermansia</subject><subject>Amplification</subject><subject>Animals</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Biosensing Techniques</subject><subject>Cost effectiveness</subject><subject>Diet, High-Fat</subject><subject>Limit of Detection</subject><subject>Mice</subject><subject>Nucleic Acid Amplification Techniques</subject><subject>Verrucomicrobia - genetics</subject><subject>Verrucomicrobia - isolation & purification</subject><issn>1359-7345</issn><issn>1364-548X</issn><issn>1364-548X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpd0U1LwzAYB_AgivPt4geQgBcRq3lt0-OYOgXBi4K3kqaJZmuTLWmF-enN3PRgICQkP_48PA8ApxhdY0TLm4YphSgSxfsOOMA0Zxln4m13fedlVlDGR-AwxhlKC3OxD0a0xEhgRg5AGEPnXabd16qTvVVX0Ebff-jQyRbKbtFaY1X68A5G7aIP0KS9HKTrrVlZ9w4ThkG3yXxqKOvBNdIpDb2B4_l8neOilbAblHV28WFbeQz2jGyjPtmeR-D1_u5l8pA9PU8fJ-OnTBHE-ozkNDcmN0znCBW8FoVkkjOCFc9rppggWklChGo4qQVh6ZXXyhjRSN0gLukRuNjkLoJfDjr2VWej0m0rnfZDrFLHSlGUhcCJnv-jMz8El6pLqmQFoWVZJHW5USr4GIM21SLYToZVhVG1nkR1yyaTn0lMEz7bRg51p5s_-tt6-g0Ms4VN</recordid><startdate>20240820</startdate><enddate>20240820</enddate><creator>Liu, Bing</creator><creator>Shi, Chen</creator><creator>Wang, Fan</creator><creator>Xu, Fangling</creator><creator>Chao, Jie</creator><creator>Zhu, Jiapeng</creator><creator>Yang, Dongliang</creator><creator>Ouyang, Xiangyuan</creator><general>Royal Society of Chemistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1030-9944</orcidid><orcidid>https://orcid.org/0000-0003-4263-7694</orcidid><orcidid>https://orcid.org/0000-0002-8605-6720</orcidid></search><sort><creationdate>20240820</creationdate><title>A non-enzymatic, isothermal amplification sensor for quantifying the relative abundance of Akkermansia muciniphila</title><author>Liu, Bing ; Shi, Chen ; Wang, Fan ; Xu, Fangling ; Chao, Jie ; Zhu, Jiapeng ; Yang, Dongliang ; Ouyang, Xiangyuan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c204t-2636ff6f4e60075b87a4a5421c56b4c482eca228cd52b82456b5bcff8daed05a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Akkermansia</topic><topic>Amplification</topic><topic>Animals</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Biosensing Techniques</topic><topic>Cost effectiveness</topic><topic>Diet, High-Fat</topic><topic>Limit of Detection</topic><topic>Mice</topic><topic>Nucleic Acid Amplification Techniques</topic><topic>Verrucomicrobia - genetics</topic><topic>Verrucomicrobia - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Bing</creatorcontrib><creatorcontrib>Shi, Chen</creatorcontrib><creatorcontrib>Wang, Fan</creatorcontrib><creatorcontrib>Xu, Fangling</creatorcontrib><creatorcontrib>Chao, Jie</creatorcontrib><creatorcontrib>Zhu, Jiapeng</creatorcontrib><creatorcontrib>Yang, Dongliang</creatorcontrib><creatorcontrib>Ouyang, Xiangyuan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Chemical communications (Cambridge, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Bing</au><au>Shi, Chen</au><au>Wang, Fan</au><au>Xu, Fangling</au><au>Chao, Jie</au><au>Zhu, Jiapeng</au><au>Yang, Dongliang</au><au>Ouyang, Xiangyuan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A non-enzymatic, isothermal amplification sensor for quantifying the relative abundance of Akkermansia muciniphila</atitle><jtitle>Chemical communications (Cambridge, England)</jtitle><addtitle>Chem Commun (Camb)</addtitle><date>2024-08-20</date><risdate>2024</risdate><volume>60</volume><issue>68</issue><spage>9089</spage><epage>9092</epage><pages>9089-9092</pages><issn>1359-7345</issn><issn>1364-548X</issn><eissn>1364-548X</eissn><abstract>Herein, we have developed a non-enzymatic, isothermal amplification assay (NIA sensor) based on a catalytic hairpin assembly (CHA) reaction for quantifying the relative abundance of
. Through detection of the MUC-1437 gene (limit of detection: 8.3 fM) in a dynamic range from 10 fM to 1 nM, the NIA sensor shows high sensitivity and selectivity in preclinical models of mice fed a normal or high-fat diet (HFD), and treated with antibiotics (ATB). The NIA sensor, which operates without the use of any enzymes, leading to simplicity and cost-effectiveness, has great potential for biosensing research and clinical diagnostic applications.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>39108142</pmid><doi>10.1039/d4cc03087g</doi><tpages>4</tpages><orcidid>https://orcid.org/0000-0003-1030-9944</orcidid><orcidid>https://orcid.org/0000-0003-4263-7694</orcidid><orcidid>https://orcid.org/0000-0002-8605-6720</orcidid></addata></record> |
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| ispartof | Chemical communications (Cambridge, England), 2024-08, Vol.60 (68), p.9089-9092 |
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| source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Akkermansia Amplification Animals Anti-Bacterial Agents - pharmacology Biosensing Techniques Cost effectiveness Diet, High-Fat Limit of Detection Mice Nucleic Acid Amplification Techniques Verrucomicrobia - genetics Verrucomicrobia - isolation & purification |
| title | A non-enzymatic, isothermal amplification sensor for quantifying the relative abundance of Akkermansia muciniphila |
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