Targeted 8‑arm PEG Nanosystems for Localization of Choroidal Neovascularization Macular Degeneration Model

Targeted 8‑arm PEG Nanosystems for Localization of Choroidal Neovascularization Macular Degeneration Model

8-arm PEG (polyethylene-glycol) is a highly promising nanoplatform due to its small size (

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Veröffentlicht in:ACS applied bio materials 2024-08, Vol.7 (8), p.5496-5505
Hauptverfasser: McLean, Alan, Zhang, Wei, Cooke, Alexander, Potter, Natalie S., Kopelman, Raoul, Paulus, Yannis M.
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container_end_page 5505
container_issue 8
container_start_page 5496
container_title ACS applied bio materials
container_volume 7
creator McLean, Alan
Zhang, Wei
Cooke, Alexander
Potter, Natalie S.
Kopelman, Raoul
Paulus, Yannis M.
description 8-arm PEG (polyethylene-glycol) is a highly promising nanoplatform due to its small size (
doi_str_mv 10.1021/acsabm.4c00628
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This study evaluates 8-arm PEG uptake into cells (in vitro) and localization and clearance in vasculature (in vivo) for targeting of choroidal neovascularization in mice, an animal model of macular degeneration. 8-arm PEG nanoparticles were labeled with fluorescein isothiocyanate (FITC) and functionalized in the absence or presence of pentameric Ar-Gly-Asp (RGD; 4 RGD motifs and a PGC linker), one of the most common peptide motifs used for active targeting. In vitro studies show that RGD-conjugated 8-arm PEG nanoparticles exhibit enhanced cellular uptake relative to non-RGD-conjugated control NPs at 34% ± 9%. Laser-induced choroidal neovascularization (CNV) was performed in a mouse model to measure 8-arm PEG localization and clearance to model macular degeneration lesions in vivo. It was determined that both RGD-conjugated and non-RGD-conjugated (nRGD) 8-arm PEG particles localized to CNV lesions, with a half-life around 24 h. In vivo experiments showed that RGD-conjugated nanoparticles exhibited enhanced localization by 15–20% relative to without RGD controls. 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Laser-induced choroidal neovascularization (CNV) was performed in a mouse model to measure 8-arm PEG localization and clearance to model macular degeneration lesions in vivo. It was determined that both RGD-conjugated and non-RGD-conjugated (nRGD) 8-arm PEG particles localized to CNV lesions, with a half-life around 24 h. In vivo experiments showed that RGD-conjugated nanoparticles exhibited enhanced localization by 15–20% relative to without RGD controls. 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title Targeted 8‑arm PEG Nanosystems for Localization of Choroidal Neovascularization Macular Degeneration Model
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