Near-Infrared Fluorescent Probe for the Detection of Cysteine
Hemicyanine dyes are an ideal structure for building near-infrared fluorescent probes due to their excellent emission wavelength properties and biocompatibility in biological imaging field. Developing a near-infrared fluorescent probe capable of detecting cysteine (Cys) was the aim of this study. A...
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| Veröffentlicht in: | Applied spectroscopy 2024-07, Vol.78 (7), p.744-752 |
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| container_title | Applied spectroscopy |
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| creator | Wang, Minghui Yang, Xindi Yuan, Mengyao Zhou, Wei Yang, Li |
| description | Hemicyanine dyes are an ideal structure for building near-infrared fluorescent probes due to their excellent emission wavelength properties and biocompatibility in biological imaging field. Developing a near-infrared fluorescent probe capable of detecting cysteine (Cys) was the aim of this study. A novel developed fluorescent probe P showed high selectivity and sensitivity to Cys in the presence of various analytes. The detection limit of P was found to be 0.329 μM. The MTT assay showed that the probe was essentially non-cytotoxic. Furthermore, the probe was successfully used as cysteine imaging in living cells and mice. |
| doi_str_mv | 10.1177/00037028241241342 |
| format | Article |
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Developing a near-infrared fluorescent probe capable of detecting cysteine (Cys) was the aim of this study. A novel developed fluorescent probe P showed high selectivity and sensitivity to Cys in the presence of various analytes. The detection limit of P was found to be 0.329 μM. The MTT assay showed that the probe was essentially non-cytotoxic. Furthermore, the probe was successfully used as cysteine imaging in living cells and mice.</description><identifier>ISSN: 0003-7028</identifier><identifier>ISSN: 1943-3530</identifier><identifier>EISSN: 1943-3530</identifier><identifier>DOI: 10.1177/00037028241241342</identifier><identifier>PMID: 39096170</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Animals ; Carbocyanines - chemistry ; Cysteine - analysis ; Cysteine - chemistry ; Fluorescent Dyes - chemistry ; HeLa Cells ; Humans ; Limit of Detection ; Mice ; Optical Imaging - methods ; Spectrometry, Fluorescence - methods ; Spectroscopy, Near-Infrared - methods</subject><ispartof>Applied spectroscopy, 2024-07, Vol.78 (7), p.744-752</ispartof><rights>The Author(s) 2024</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c292t-61115da291cf1ea3283f7986b3d595fa346313e30d26b06066fc44a2692377563</cites><orcidid>0009-0005-5230-5257</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/00037028241241342$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/00037028241241342$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,780,784,21810,27915,27916,43612,43613</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39096170$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Minghui</creatorcontrib><creatorcontrib>Yang, Xindi</creatorcontrib><creatorcontrib>Yuan, Mengyao</creatorcontrib><creatorcontrib>Zhou, Wei</creatorcontrib><creatorcontrib>Yang, Li</creatorcontrib><title>Near-Infrared Fluorescent Probe for the Detection of Cysteine</title><title>Applied spectroscopy</title><addtitle>Appl Spectrosc</addtitle><description>Hemicyanine dyes are an ideal structure for building near-infrared fluorescent probes due to their excellent emission wavelength properties and biocompatibility in biological imaging field. Developing a near-infrared fluorescent probe capable of detecting cysteine (Cys) was the aim of this study. A novel developed fluorescent probe P showed high selectivity and sensitivity to Cys in the presence of various analytes. The detection limit of P was found to be 0.329 μM. The MTT assay showed that the probe was essentially non-cytotoxic. Furthermore, the probe was successfully used as cysteine imaging in living cells and mice.</description><subject>Animals</subject><subject>Carbocyanines - chemistry</subject><subject>Cysteine - analysis</subject><subject>Cysteine - chemistry</subject><subject>Fluorescent Dyes - chemistry</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Limit of Detection</subject><subject>Mice</subject><subject>Optical Imaging - methods</subject><subject>Spectrometry, Fluorescence - methods</subject><subject>Spectroscopy, Near-Infrared - methods</subject><issn>0003-7028</issn><issn>1943-3530</issn><issn>1943-3530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kDFPwzAQhS0EoqXwA1hQRpYU2xfb8cCACoVKFTDAHDnJGVKlcbGdof-eVC0sSEgnnU73vae7R8glo1PGlLqhlIKiPOcZGwoyfkTGTGeQggB6TMa7fboDRuQshNUwCg3ilIxAUy2ZomNy-4zGp4vOeuOxTuZt7zyGCruYvHpXYmKdT-InJvcYsYqN6xJnk9k2RGw6PCcn1rQBLw59Qt7nD2-zp3T58riY3S3TimseU8kYE7XhmlWWoQGeg1U6lyXUQgtrIJPAAIHWXJZUUiltlWWGS81BKSFhQq73vhvvvnoMsVg3w5Ftazp0fSiA5krqXOdiQNkerbwLwaMtNr5ZG78tGC12qRV_Uhs0Vwf7vlxj_av4iWkApnsgmA8sVq733fDuP47fpwpxfA</recordid><startdate>20240701</startdate><enddate>20240701</enddate><creator>Wang, Minghui</creator><creator>Yang, Xindi</creator><creator>Yuan, Mengyao</creator><creator>Zhou, Wei</creator><creator>Yang, Li</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0009-0005-5230-5257</orcidid></search><sort><creationdate>20240701</creationdate><title>Near-Infrared Fluorescent Probe for the Detection of Cysteine</title><author>Wang, Minghui ; Yang, Xindi ; Yuan, Mengyao ; Zhou, Wei ; Yang, Li</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c292t-61115da291cf1ea3283f7986b3d595fa346313e30d26b06066fc44a2692377563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Animals</topic><topic>Carbocyanines - chemistry</topic><topic>Cysteine - analysis</topic><topic>Cysteine - chemistry</topic><topic>Fluorescent Dyes - chemistry</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Limit of Detection</topic><topic>Mice</topic><topic>Optical Imaging - methods</topic><topic>Spectrometry, Fluorescence - methods</topic><topic>Spectroscopy, Near-Infrared - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Minghui</creatorcontrib><creatorcontrib>Yang, Xindi</creatorcontrib><creatorcontrib>Yuan, Mengyao</creatorcontrib><creatorcontrib>Zhou, Wei</creatorcontrib><creatorcontrib>Yang, Li</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Applied spectroscopy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Minghui</au><au>Yang, Xindi</au><au>Yuan, Mengyao</au><au>Zhou, Wei</au><au>Yang, Li</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Near-Infrared Fluorescent Probe for the Detection of Cysteine</atitle><jtitle>Applied spectroscopy</jtitle><addtitle>Appl Spectrosc</addtitle><date>2024-07-01</date><risdate>2024</risdate><volume>78</volume><issue>7</issue><spage>744</spage><epage>752</epage><pages>744-752</pages><issn>0003-7028</issn><issn>1943-3530</issn><eissn>1943-3530</eissn><abstract>Hemicyanine dyes are an ideal structure for building near-infrared fluorescent probes due to their excellent emission wavelength properties and biocompatibility in biological imaging field. Developing a near-infrared fluorescent probe capable of detecting cysteine (Cys) was the aim of this study. A novel developed fluorescent probe P showed high selectivity and sensitivity to Cys in the presence of various analytes. The detection limit of P was found to be 0.329 μM. The MTT assay showed that the probe was essentially non-cytotoxic. Furthermore, the probe was successfully used as cysteine imaging in living cells and mice.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>39096170</pmid><doi>10.1177/00037028241241342</doi><tpages>9</tpages><orcidid>https://orcid.org/0009-0005-5230-5257</orcidid></addata></record> |
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| ispartof | Applied spectroscopy, 2024-07, Vol.78 (7), p.744-752 |
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| language | eng |
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| source | MEDLINE; SAGE Complete A-Z List |
| subjects | Animals Carbocyanines - chemistry Cysteine - analysis Cysteine - chemistry Fluorescent Dyes - chemistry HeLa Cells Humans Limit of Detection Mice Optical Imaging - methods Spectrometry, Fluorescence - methods Spectroscopy, Near-Infrared - methods |
| title | Near-Infrared Fluorescent Probe for the Detection of Cysteine |
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