Persistence and pathway of glyphosate degradation in the coastal wetland soil of central Delaware
Glyphosate is a globally dominant herbicide. Here, we studied the degradation and microbial response to glyphosate application in a wetland soil in central Delaware for controlling invasive species (Phragmites australis). We applied a two-step solid-phase extraction method using molecularly imprinte...
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| Veröffentlicht in: | Journal of hazardous materials 2024-09, Vol.477, p.135238, Article 135238 |
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| creator | Moller, Spencer R. Campos, Marco A. Rilling, Joaquin I. Bakkour, Rani Hollenback, Anthony J. Jorquera, Milko A. Jaisi, Deb P. |
| description | Glyphosate is a globally dominant herbicide. Here, we studied the degradation and microbial response to glyphosate application in a wetland soil in central Delaware for controlling invasive species (Phragmites australis). We applied a two-step solid-phase extraction method using molecularly imprinted polymers designed for the separation and enrichment of glyphosate and aminomethylphosphonic acid (AMPA) from soils before their analysis by ultra-high-performance liquid chromatography (UHPLC) and Q Exactive Orbitrap mass spectrometry methods. Our results showed that approximately 90 % of glyphosate degraded over 100 d after application, with AMPA being a minor ( |
| doi_str_mv | 10.1016/j.jhazmat.2024.135238 |
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[Display omitted]
•Identified the occurrence of benign degradation pathways of glyphosate in soils.•Expression of the phnJ gene confirmed the dominance of C-P bond cleavage.•Suppressing AMPA production is most favored for protecting the environment.</description><identifier>ISSN: 0304-3894</identifier><identifier>ISSN: 1873-3336</identifier><identifier>EISSN: 1873-3336</identifier><identifier>DOI: 10.1016/j.jhazmat.2024.135238</identifier><identifier>PMID: 39096637</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Aminomethylphosphonic acid (AMPA) ; Biodegradation, Environmental ; Degradation pathway ; Delaware ; Gene abundance ; Gene expression ; Glycine - analogs & derivatives ; Glycine - metabolism ; Glyphosate ; Herbicides - chemistry ; Herbicides - metabolism ; Isoxazoles - metabolism ; Lyases - genetics ; Lyases - metabolism ; Orbitrap MS ; Organophosphonates - metabolism ; PhnJ gene ; Soil Microbiology ; Soil Pollutants - metabolism ; Tetrazoles ; UHPLC ; Wetlands</subject><ispartof>Journal of hazardous materials, 2024-09, Vol.477, p.135238, Article 135238</ispartof><rights>2024 Elsevier B.V.</rights><rights>Copyright © 2024 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c243t-f5e04ddb3be94c6fd5027646d6d9cd8d500ba3ab606186f56087ea1d0bcc01f63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jhazmat.2024.135238$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39096637$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Moller, Spencer R.</creatorcontrib><creatorcontrib>Campos, Marco A.</creatorcontrib><creatorcontrib>Rilling, Joaquin I.</creatorcontrib><creatorcontrib>Bakkour, Rani</creatorcontrib><creatorcontrib>Hollenback, Anthony J.</creatorcontrib><creatorcontrib>Jorquera, Milko A.</creatorcontrib><creatorcontrib>Jaisi, Deb P.</creatorcontrib><title>Persistence and pathway of glyphosate degradation in the coastal wetland soil of central Delaware</title><title>Journal of hazardous materials</title><addtitle>J Hazard Mater</addtitle><description>Glyphosate is a globally dominant herbicide. Here, we studied the degradation and microbial response to glyphosate application in a wetland soil in central Delaware for controlling invasive species (Phragmites australis). We applied a two-step solid-phase extraction method using molecularly imprinted polymers designed for the separation and enrichment of glyphosate and aminomethylphosphonic acid (AMPA) from soils before their analysis by ultra-high-performance liquid chromatography (UHPLC) and Q Exactive Orbitrap mass spectrometry methods. Our results showed that approximately 90 % of glyphosate degraded over 100 d after application, with AMPA being a minor (<10 %) product. Analysis of glyphosate-specific microbial genes to identify microbial response and function revealed that the expression of the phnJ gene, which codes C-P lyase enzyme, was consistently dominant over the gox gene, which codes glyphosate oxidoreductase enzyme, after glyphosate application. Both gene and concentration data independently suggested that C-P bond cleavage—which forms sarcosine or glycine—was the dominant degradation pathway. This is significant because AMPA, a more toxic product, is reported to be the preferred pathway of glyphosate degradation in other soil and natural environments. The degradation through a safer pathway is encouraging for minimizing the detrimental impacts of glyphosate on the environment.
[Display omitted]
•Identified the occurrence of benign degradation pathways of glyphosate in soils.•Expression of the phnJ gene confirmed the dominance of C-P bond cleavage.•Suppressing AMPA production is most favored for protecting the environment.</description><subject>Aminomethylphosphonic acid (AMPA)</subject><subject>Biodegradation, Environmental</subject><subject>Degradation pathway</subject><subject>Delaware</subject><subject>Gene abundance</subject><subject>Gene expression</subject><subject>Glycine - analogs & derivatives</subject><subject>Glycine - metabolism</subject><subject>Glyphosate</subject><subject>Herbicides - chemistry</subject><subject>Herbicides - metabolism</subject><subject>Isoxazoles - metabolism</subject><subject>Lyases - genetics</subject><subject>Lyases - metabolism</subject><subject>Orbitrap MS</subject><subject>Organophosphonates - metabolism</subject><subject>PhnJ gene</subject><subject>Soil Microbiology</subject><subject>Soil Pollutants - metabolism</subject><subject>Tetrazoles</subject><subject>UHPLC</subject><subject>Wetlands</subject><issn>0304-3894</issn><issn>1873-3336</issn><issn>1873-3336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtu2zAQRYkiQeOm_YQEXGYjdyhKlLQKgjzaAgbSRbMmRuQopiGLDknXcL4-EuxkmxUxxD3zOIxdCJgLEOrnar5a4usa0zyHvJgLWeay_sJmoq5kJqVUJ2wGEopM1k1xxr7FuAIAUZXFV3YmG2iUktWM4V8K0cVEgyGOg-UbTMsd7rnv-HO_3yx9xETc0nNAi8n5gbuBpyVx4zEm7PmOUj-B0bt-ogwNKYz_d9TjDgN9Z6cd9pF-HN9z9vRw_-_2d7Z4_PXn9maRmbyQKetKgsLaVrbUFEZ1toS8UoWyyjbG1mMJLUpsFShRq65UUFeEwkJrDIhOyXN2dei7Cf5lSzHptYuG-nE58tuo5QiopoayHqPlIWqCjzFQpzfBrTHstQA92dUrfbSrJ7v6YHfkLo8jtu2a7Af1rnMMXB8CNB7631HQ0bhJrXWBTNLWu09GvAHbFo90</recordid><startdate>20240915</startdate><enddate>20240915</enddate><creator>Moller, Spencer R.</creator><creator>Campos, Marco A.</creator><creator>Rilling, Joaquin I.</creator><creator>Bakkour, Rani</creator><creator>Hollenback, Anthony J.</creator><creator>Jorquera, Milko A.</creator><creator>Jaisi, Deb P.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20240915</creationdate><title>Persistence and pathway of glyphosate degradation in the coastal wetland soil of central Delaware</title><author>Moller, Spencer R. ; Campos, Marco A. ; Rilling, Joaquin I. ; Bakkour, Rani ; Hollenback, Anthony J. ; Jorquera, Milko A. ; Jaisi, Deb P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c243t-f5e04ddb3be94c6fd5027646d6d9cd8d500ba3ab606186f56087ea1d0bcc01f63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Aminomethylphosphonic acid (AMPA)</topic><topic>Biodegradation, Environmental</topic><topic>Degradation pathway</topic><topic>Delaware</topic><topic>Gene abundance</topic><topic>Gene expression</topic><topic>Glycine - analogs & derivatives</topic><topic>Glycine - metabolism</topic><topic>Glyphosate</topic><topic>Herbicides - chemistry</topic><topic>Herbicides - metabolism</topic><topic>Isoxazoles - metabolism</topic><topic>Lyases - genetics</topic><topic>Lyases - metabolism</topic><topic>Orbitrap MS</topic><topic>Organophosphonates - metabolism</topic><topic>PhnJ gene</topic><topic>Soil Microbiology</topic><topic>Soil Pollutants - metabolism</topic><topic>Tetrazoles</topic><topic>UHPLC</topic><topic>Wetlands</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Moller, Spencer R.</creatorcontrib><creatorcontrib>Campos, Marco A.</creatorcontrib><creatorcontrib>Rilling, Joaquin I.</creatorcontrib><creatorcontrib>Bakkour, Rani</creatorcontrib><creatorcontrib>Hollenback, Anthony J.</creatorcontrib><creatorcontrib>Jorquera, Milko A.</creatorcontrib><creatorcontrib>Jaisi, Deb P.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of hazardous materials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Moller, Spencer R.</au><au>Campos, Marco A.</au><au>Rilling, Joaquin I.</au><au>Bakkour, Rani</au><au>Hollenback, Anthony J.</au><au>Jorquera, Milko A.</au><au>Jaisi, Deb P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Persistence and pathway of glyphosate degradation in the coastal wetland soil of central Delaware</atitle><jtitle>Journal of hazardous materials</jtitle><addtitle>J Hazard Mater</addtitle><date>2024-09-15</date><risdate>2024</risdate><volume>477</volume><spage>135238</spage><pages>135238-</pages><artnum>135238</artnum><issn>0304-3894</issn><issn>1873-3336</issn><eissn>1873-3336</eissn><abstract>Glyphosate is a globally dominant herbicide. Here, we studied the degradation and microbial response to glyphosate application in a wetland soil in central Delaware for controlling invasive species (Phragmites australis). We applied a two-step solid-phase extraction method using molecularly imprinted polymers designed for the separation and enrichment of glyphosate and aminomethylphosphonic acid (AMPA) from soils before their analysis by ultra-high-performance liquid chromatography (UHPLC) and Q Exactive Orbitrap mass spectrometry methods. Our results showed that approximately 90 % of glyphosate degraded over 100 d after application, with AMPA being a minor (<10 %) product. Analysis of glyphosate-specific microbial genes to identify microbial response and function revealed that the expression of the phnJ gene, which codes C-P lyase enzyme, was consistently dominant over the gox gene, which codes glyphosate oxidoreductase enzyme, after glyphosate application. Both gene and concentration data independently suggested that C-P bond cleavage—which forms sarcosine or glycine—was the dominant degradation pathway. This is significant because AMPA, a more toxic product, is reported to be the preferred pathway of glyphosate degradation in other soil and natural environments. The degradation through a safer pathway is encouraging for minimizing the detrimental impacts of glyphosate on the environment.
[Display omitted]
•Identified the occurrence of benign degradation pathways of glyphosate in soils.•Expression of the phnJ gene confirmed the dominance of C-P bond cleavage.•Suppressing AMPA production is most favored for protecting the environment.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>39096637</pmid><doi>10.1016/j.jhazmat.2024.135238</doi></addata></record> |
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| subjects | Aminomethylphosphonic acid (AMPA) Biodegradation, Environmental Degradation pathway Delaware Gene abundance Gene expression Glycine - analogs & derivatives Glycine - metabolism Glyphosate Herbicides - chemistry Herbicides - metabolism Isoxazoles - metabolism Lyases - genetics Lyases - metabolism Orbitrap MS Organophosphonates - metabolism PhnJ gene Soil Microbiology Soil Pollutants - metabolism Tetrazoles UHPLC Wetlands |
| title | Persistence and pathway of glyphosate degradation in the coastal wetland soil of central Delaware |
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