PCIS1, Encoded by a Pentatricopeptide Protein Co-expressed Gene, Is Required for Splicing of Three Mitochondrial nad Transcripts in Angiosperms

Group II introns are large catalytic RNAs, which reside mainly within genes encoding respiratory complex I (CI) subunits in angiosperms' mitochondria. Genetic and biochemical analyses led to the identification of many nuclear-encoded factors that facilitate the splicing of the degenerated organ...

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Veröffentlicht in:Plant and cell physiology 2024-10, Vol.65 (9), p.1474-1485
Hauptverfasser: Frink, Brody, Burger, Matthias, Yarkoni, Maya, Shevtsov-Tal, Sofia, Zer, Hagit, Yamaoka, Shohei, Ostersetzer-Biran, Oren, Takenaka, Mizuki
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container_end_page 1485
container_issue 9
container_start_page 1474
container_title Plant and cell physiology
container_volume 65
creator Frink, Brody
Burger, Matthias
Yarkoni, Maya
Shevtsov-Tal, Sofia
Zer, Hagit
Yamaoka, Shohei
Ostersetzer-Biran, Oren
Takenaka, Mizuki
description Group II introns are large catalytic RNAs, which reside mainly within genes encoding respiratory complex I (CI) subunits in angiosperms' mitochondria. Genetic and biochemical analyses led to the identification of many nuclear-encoded factors that facilitate the splicing of the degenerated organellar introns in plants. Here, we describe the analysis of the pentatricopeptide repeat (PPR) co-expressed intron splicing-1 (PCIS1) factor, which was identified in silico by its co-expression pattern with many PPR proteins. PCIS1 is well conserved in land plants but has no sequence similarity with any known protein motifs. PCIS1 mutant lines are arrested in embryogenesis and can be maintained by the temporal expression of the gene under the embryo-specific ABI3 promoter. The pABI3::PCIS1 mutant plants display low germination and stunted growth phenotypes. RNA-sequencing and quantitative RT-PCR analyses of wild-type and mutant plants indicated that PCIS1 is a novel splicing cofactor that is pivotal for the maturation of several nad transcripts in Arabidopsis mitochondria. These phenotypes are tightly associated with respiratory CI defects and altered plant growth. Our data further emphasize the key roles of nuclear-encoded cofactors that regulate the maturation and expression of mitochondrial transcripts for the biogenesis of the oxidative phosphorylation system, and hence for plant physiology. The discovery of novel splicing factors other than typical RNA-binding proteins suggests further complexity of splicing mechanisms in plant mitochondria.
doi_str_mv 10.1093/pcp/pcae086
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source MEDLINE; Oxford University Press Journals All Titles (1996-Current)
subjects Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Electron Transport Complex I - genetics
Electron Transport Complex I - metabolism
Gene Expression Regulation, Plant
Introns - genetics
Mitochondria - genetics
Mitochondria - metabolism
Mitochondrial Proteins - genetics
Mitochondrial Proteins - metabolism
Mutation - genetics
NAD - metabolism
RNA Splicing - genetics
title PCIS1, Encoded by a Pentatricopeptide Protein Co-expressed Gene, Is Required for Splicing of Three Mitochondrial nad Transcripts in Angiosperms
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