Development of a thermostable Cre/lox-based gene disruption system and in vivo manipulations of the megaplasmid pTT27 in Thermus thermophilus HB27

We previously reported the development of a Cre/lox-based gene disruption system for multiple markerless gene disruption in Thermus thermophilus; however, it was a time-consuming method because it functioned at 50 °C, the minimum growth temperature of T. thermophilus HB27. In the present study, we i...

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Veröffentlicht in:	Plasmid 2024-09, Vol.131-132, p.102730, Article 102730
Hauptverfasser:	Hiratsu, Keiichiro, Nunoshiba, Tatsuo, Togawa, Yoichiro, Yamauchi, Yoshito
Format:	Artikel
Sprache:	eng
Schlagworte:	Cre/lox system Integrases - genetics Integrases - metabolism Markerless gene disruption Plasmids - genetics Plasmids - metabolism Recombination, Genetic Temperature Thermus thermophilus Thermus thermophilus - genetics Thermus thermophilus - metabolism β-galactosidase assay
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