Highland Barley β‑Glucan Relieves Symptoms of Colitis via PPARα-Mediated Intestinal Stem Cell Proliferation
Intestinal stem cells (ISCs) are necessary to maintain intestinal renewal. Here, we found that the highland barley β-glucan (HBG) alleviated pathological symptoms and promoted the proliferation of intestinal stem cells in colitis mice. Notably, metabolomics studies showed that docosahexaenoic acid (...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2024-11, Vol.72 (44), p.24359-24373 |
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| container_issue | 44 |
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| container_title | Journal of agricultural and food chemistry |
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| creator | Yu, Yongkang Zhang, Ke Zhang, Duoduo Feng, Ruting Chen, Kunying Zhou, Xingtao Nie, Shaoping Xie, Ming-Yong |
| description | Intestinal stem cells (ISCs) are necessary to maintain intestinal renewal. Here, we found that the highland barley β-glucan (HBG) alleviated pathological symptoms and promoted the proliferation of intestinal stem cells in colitis mice. Notably, metabolomics studies showed that docosahexaenoic acid (DHA) was significantly increased by the HBG treatment. DHA is a ligand for peroxisome proliferator-activated receptor α (PPARα), which can promote ISC proliferation. Expectedly, HBG facilitated the expression of intestinal PPARα and the proliferation of ISCs in colitis mice. Further experiments verified that DHA significantly facilitated the expression of PPARα and the proliferation of ISCs in intestinal organoids. Intriguingly, the effect of DHA on ISC proliferation was reversed by the PPARα inhibitor. Together, our data indicate that HBG might accelerate PPARα-mediated ISC proliferation through DHA. This provides new insights into the effective application of polysaccharides in maintaining intestinal homeostasis. |
| doi_str_mv | 10.1021/acs.jafc.3c09535 |
| format | Article |
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Here, we found that the highland barley β-glucan (HBG) alleviated pathological symptoms and promoted the proliferation of intestinal stem cells in colitis mice. Notably, metabolomics studies showed that docosahexaenoic acid (DHA) was significantly increased by the HBG treatment. DHA is a ligand for peroxisome proliferator-activated receptor α (PPARα), which can promote ISC proliferation. Expectedly, HBG facilitated the expression of intestinal PPARα and the proliferation of ISCs in colitis mice. Further experiments verified that DHA significantly facilitated the expression of PPARα and the proliferation of ISCs in intestinal organoids. Intriguingly, the effect of DHA on ISC proliferation was reversed by the PPARα inhibitor. Together, our data indicate that HBG might accelerate PPARα-mediated ISC proliferation through DHA. This provides new insights into the effective application of polysaccharides in maintaining intestinal homeostasis.</description><identifier>ISSN: 0021-8561</identifier><identifier>ISSN: 1520-5118</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/acs.jafc.3c09535</identifier><identifier>PMID: 39084686</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Animals ; beta-Glucans - pharmacology ; Bioactive Constituents, Metabolites, and Functions ; Cell Proliferation - drug effects ; Colitis - chemically induced ; Colitis - drug therapy ; Colitis - metabolism ; Docosahexaenoic Acids - pharmacology ; Hordeum - chemistry ; Humans ; Intestinal Mucosa - cytology ; Intestinal Mucosa - drug effects ; Intestinal Mucosa - metabolism ; Intestines - cytology ; Intestines - drug effects ; Male ; Mice ; Mice, Inbred C57BL ; PPAR alpha - genetics ; PPAR alpha - metabolism ; Stem Cells - cytology ; Stem Cells - drug effects ; Stem Cells - metabolism</subject><ispartof>Journal of agricultural and food chemistry, 2024-11, Vol.72 (44), p.24359-24373</ispartof><rights>2024 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-a219t-c63d661e7fdbe3ff37a6a9c3a81c2b771a694df9fb4aea8f667a8bae7af8fb893</cites><orcidid>0000-0003-3004-7638</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.jafc.3c09535$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.jafc.3c09535$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39084686$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yu, Yongkang</creatorcontrib><creatorcontrib>Zhang, Ke</creatorcontrib><creatorcontrib>Zhang, Duoduo</creatorcontrib><creatorcontrib>Feng, Ruting</creatorcontrib><creatorcontrib>Chen, Kunying</creatorcontrib><creatorcontrib>Zhou, Xingtao</creatorcontrib><creatorcontrib>Nie, Shaoping</creatorcontrib><creatorcontrib>Xie, Ming-Yong</creatorcontrib><title>Highland Barley β‑Glucan Relieves Symptoms of Colitis via PPARα-Mediated Intestinal Stem Cell Proliferation</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>Intestinal stem cells (ISCs) are necessary to maintain intestinal renewal. Here, we found that the highland barley β-glucan (HBG) alleviated pathological symptoms and promoted the proliferation of intestinal stem cells in colitis mice. Notably, metabolomics studies showed that docosahexaenoic acid (DHA) was significantly increased by the HBG treatment. DHA is a ligand for peroxisome proliferator-activated receptor α (PPARα), which can promote ISC proliferation. Expectedly, HBG facilitated the expression of intestinal PPARα and the proliferation of ISCs in colitis mice. Further experiments verified that DHA significantly facilitated the expression of PPARα and the proliferation of ISCs in intestinal organoids. Intriguingly, the effect of DHA on ISC proliferation was reversed by the PPARα inhibitor. Together, our data indicate that HBG might accelerate PPARα-mediated ISC proliferation through DHA. This provides new insights into the effective application of polysaccharides in maintaining intestinal homeostasis.</description><subject>Animals</subject><subject>beta-Glucans - pharmacology</subject><subject>Bioactive Constituents, Metabolites, and Functions</subject><subject>Cell Proliferation - drug effects</subject><subject>Colitis - chemically induced</subject><subject>Colitis - drug therapy</subject><subject>Colitis - metabolism</subject><subject>Docosahexaenoic Acids - pharmacology</subject><subject>Hordeum - chemistry</subject><subject>Humans</subject><subject>Intestinal Mucosa - cytology</subject><subject>Intestinal Mucosa - drug effects</subject><subject>Intestinal Mucosa - metabolism</subject><subject>Intestines - cytology</subject><subject>Intestines - drug effects</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>PPAR alpha - genetics</subject><subject>PPAR alpha - metabolism</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - drug effects</subject><subject>Stem Cells - metabolism</subject><issn>0021-8561</issn><issn>1520-5118</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kL1u2zAURomiReKm2TsVHDtELmlaFDWmRv4ABzHyMwtX1GXLgBJdUjLgLa-QR0kfxA-RJwkTu9k63eWcDxeHkK-cjTmb8B-g4_gejB4Lzcpc5B_IiOcTluWcq49kxBKTqVzyffI5xnvGmMoLtkf2RcnUVCo5Iv7c_vrtoGvoTwgO13Tz9_nh8cwNGjp6jc7iCiO9WbfL3reRekNn3tneRrqyQBeL4-vNU3aJjYUeG3rR9Rh724GjNz22dIbO0UVIhsEAvfXdF_LJgIt4uLsH5O705HZ2ns2vzi5mx_MMJrzsMy1FIyXHwjQ1CmNEARJKLUBxPamLgoMsp40pTT0FBGWkLEDVgAUYZWpVigPyfbu7DP7PkJ6qWht1egc69EOsBFOyzKcFEwllW1QHH2NAUy2DbSGsK86q18xVyly9Zq52mZPybbc-1C0278K_rgk42gJvqh9CShL_v_cCvYWNdA</recordid><startdate>20241106</startdate><enddate>20241106</enddate><creator>Yu, Yongkang</creator><creator>Zhang, Ke</creator><creator>Zhang, Duoduo</creator><creator>Feng, Ruting</creator><creator>Chen, Kunying</creator><creator>Zhou, Xingtao</creator><creator>Nie, Shaoping</creator><creator>Xie, Ming-Yong</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-3004-7638</orcidid></search><sort><creationdate>20241106</creationdate><title>Highland Barley β‑Glucan Relieves Symptoms of Colitis via PPARα-Mediated Intestinal Stem Cell Proliferation</title><author>Yu, Yongkang ; Zhang, Ke ; Zhang, Duoduo ; Feng, Ruting ; Chen, Kunying ; Zhou, Xingtao ; Nie, Shaoping ; Xie, Ming-Yong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a219t-c63d661e7fdbe3ff37a6a9c3a81c2b771a694df9fb4aea8f667a8bae7af8fb893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Animals</topic><topic>beta-Glucans - pharmacology</topic><topic>Bioactive Constituents, Metabolites, and Functions</topic><topic>Cell Proliferation - drug effects</topic><topic>Colitis - chemically induced</topic><topic>Colitis - drug therapy</topic><topic>Colitis - metabolism</topic><topic>Docosahexaenoic Acids - pharmacology</topic><topic>Hordeum - chemistry</topic><topic>Humans</topic><topic>Intestinal Mucosa - cytology</topic><topic>Intestinal Mucosa - drug effects</topic><topic>Intestinal Mucosa - metabolism</topic><topic>Intestines - cytology</topic><topic>Intestines - drug effects</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>PPAR alpha - genetics</topic><topic>PPAR alpha - metabolism</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - drug effects</topic><topic>Stem Cells - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yu, Yongkang</creatorcontrib><creatorcontrib>Zhang, Ke</creatorcontrib><creatorcontrib>Zhang, Duoduo</creatorcontrib><creatorcontrib>Feng, Ruting</creatorcontrib><creatorcontrib>Chen, Kunying</creatorcontrib><creatorcontrib>Zhou, Xingtao</creatorcontrib><creatorcontrib>Nie, Shaoping</creatorcontrib><creatorcontrib>Xie, Ming-Yong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yu, Yongkang</au><au>Zhang, Ke</au><au>Zhang, Duoduo</au><au>Feng, Ruting</au><au>Chen, Kunying</au><au>Zhou, Xingtao</au><au>Nie, Shaoping</au><au>Xie, Ming-Yong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Highland Barley β‑Glucan Relieves Symptoms of Colitis via PPARα-Mediated Intestinal Stem Cell Proliferation</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2024-11-06</date><risdate>2024</risdate><volume>72</volume><issue>44</issue><spage>24359</spage><epage>24373</epage><pages>24359-24373</pages><issn>0021-8561</issn><issn>1520-5118</issn><eissn>1520-5118</eissn><abstract>Intestinal stem cells (ISCs) are necessary to maintain intestinal renewal. Here, we found that the highland barley β-glucan (HBG) alleviated pathological symptoms and promoted the proliferation of intestinal stem cells in colitis mice. Notably, metabolomics studies showed that docosahexaenoic acid (DHA) was significantly increased by the HBG treatment. DHA is a ligand for peroxisome proliferator-activated receptor α (PPARα), which can promote ISC proliferation. Expectedly, HBG facilitated the expression of intestinal PPARα and the proliferation of ISCs in colitis mice. Further experiments verified that DHA significantly facilitated the expression of PPARα and the proliferation of ISCs in intestinal organoids. Intriguingly, the effect of DHA on ISC proliferation was reversed by the PPARα inhibitor. Together, our data indicate that HBG might accelerate PPARα-mediated ISC proliferation through DHA. This provides new insights into the effective application of polysaccharides in maintaining intestinal homeostasis.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>39084686</pmid><doi>10.1021/acs.jafc.3c09535</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0003-3004-7638</orcidid></addata></record> |
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| subjects | Animals beta-Glucans - pharmacology Bioactive Constituents, Metabolites, and Functions Cell Proliferation - drug effects Colitis - chemically induced Colitis - drug therapy Colitis - metabolism Docosahexaenoic Acids - pharmacology Hordeum - chemistry Humans Intestinal Mucosa - cytology Intestinal Mucosa - drug effects Intestinal Mucosa - metabolism Intestines - cytology Intestines - drug effects Male Mice Mice, Inbred C57BL PPAR alpha - genetics PPAR alpha - metabolism Stem Cells - cytology Stem Cells - drug effects Stem Cells - metabolism |
| title | Highland Barley β‑Glucan Relieves Symptoms of Colitis via PPARα-Mediated Intestinal Stem Cell Proliferation |
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