Comparative analysis of cyanobacterial communities in gypsum outcrops: insights from sites in Israel and Poland
Today, the biodiversity of endolithic microbial colonisations are only partly understood. In this study, we used a combination of molecular community metabarcoding using the 16S rRNA gene, light microscopy, CT-scan analysis, and Raman spectroscopy to describe gypsum endolithic communities in 2 sites...
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| Veröffentlicht in: | Extremophiles : life under extreme conditions 2024-12, Vol.28 (3), p.37, Article 37 |
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| creator | Němečková, Kateřina Mareš, Jan Košek, Filip Culka, Adam Dudák, Jan Tymlová, Veronika Žemlička, Jan Jehlička, Jan |
| description | Today, the biodiversity of endolithic microbial colonisations are only partly understood. In this study, we used a combination of molecular community metabarcoding using the 16S rRNA gene, light microscopy, CT-scan analysis, and Raman spectroscopy to describe gypsum endolithic communities in 2 sites—southern Poland and northern Israel. The obtained results have shown that despite different geographical areas, climatic conditions, and also physical features of colonized gypsum outcrops, both of these sites have remarkably similar microbial and pigment compositions. Cyanobacteria dominate both of the gypsum habitats, followed by Chloroflexi and Pseudomonadota. Among cyanobacteria, Thermosynechococcaceae were more abundant in Israel while Chroococcidiopsidaceae in Poland. Interestingly, no Gloeobacteraceae sequences have been found in Poland, only in Israel. Some of the obtained 16S rRNA gene sequences of cyanobacteria matched previously detected sequences from endolithic communities in various substrates and geographical regions, supporting the hypothesis of global metacommunity, but more data are still needed. Using Raman spectroscopy, cyanobacterial UV-screening pigments—scytonemin and gloeocapsin have been detected alongside carotenoids, chlorophyll
a
and melanin. These pigments can serve as potential biomarkers for basic taxonomic identification of cyanobacteria. Overall, this study provides more insight into the diversity of cyanobacterial endolithic colonisations in gypsum across different areas. |
| doi_str_mv | 10.1007/s00792-024-01352-4 |
| format | Article |
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a
and melanin. These pigments can serve as potential biomarkers for basic taxonomic identification of cyanobacteria. 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a
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| ispartof | Extremophiles : life under extreme conditions, 2024-12, Vol.28 (3), p.37, Article 37 |
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| subjects | Biochemistry Biodiversity Biomarkers Biomedical and Life Sciences Biotechnology Calcium Sulfate - chemistry Carotenoids Chlorophyll a Climatic conditions Comparative analysis Computed tomography Cyanobacteria Cyanobacteria - classification Cyanobacteria - genetics Cyanobacteria - metabolism Gene sequencing Gypsum Israel Life Sciences Light microscopy Melanin Microbial Ecology Microbiology Microbiota Microorganisms Optical microscopy Original Paper Outcrops Pigments Poland Raman spectroscopy RNA, Ribosomal, 16S - genetics rRNA 16S Spectroscopy Spectrum analysis Substrates |
| title | Comparative analysis of cyanobacterial communities in gypsum outcrops: insights from sites in Israel and Poland |
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