Size exclusion chromatography and asymmetrical flow field-flow fractionation for structural characterization of polysaccharides: A comparative review
Natural polysaccharides exhibit a wide range of biological activities, which are closely related to their structural characteristics, including their molecular weight distribution, size, monosaccharide composition, glycosidic bond types and spatial conformation, etc. Size exclusion chromatography (S...
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| Veröffentlicht in: | International journal of biological macromolecules 2024-10, Vol.277 (Pt 2), p.134236, Article 134236 |
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| container_issue | Pt 2 |
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| container_title | International journal of biological macromolecules |
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| creator | Chen, Zehong Wang, Dan Gu, Saisai Wu, Niuniu Wang, Kaiping Zhang, Yu |
| description | Natural polysaccharides exhibit a wide range of biological activities, which are closely related to their structural characteristics, including their molecular weight distribution, size, monosaccharide composition, glycosidic bond types and spatial conformation, etc. Size exclusion chromatography (SEC) and asymmetrical flow field-flow fractionation (AF4), as two potent separation techniques, both harbor potential for continuous development and enhancement. This manuscript reviewed the fundamental principles and separation applications of SEC and AF4. The structural information and spatial conformation of polysaccharides can be obtained using SEC or AF4 coupled with multiple detectors. In addition, this manuscript elaborates in detail on the shear degradation of samples such as polysaccharides separated by SEC. In addition, the abnormal elution that occurs during the application of the two methods is also discussed. Both SEC and AF4 possess considerable potential for ongoing development and refinement, thereby offering increased possibilities and opportunities for polysaccharide separation and characterization. |
| doi_str_mv | 10.1016/j.ijbiomac.2024.134236 |
| format | Article |
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Both SEC and AF4 possess considerable potential for ongoing development and refinement, thereby offering increased possibilities and opportunities for polysaccharide separation and characterization.</description><subject>Abnormal elution</subject><subject>AF4</subject><subject>Chromatography, Gel - methods</subject><subject>Fractionation, Field Flow - methods</subject><subject>Molecular Weight</subject><subject>Monosaccharides - analysis</subject><subject>Monosaccharides - chemistry</subject><subject>Polysaccharide</subject><subject>Polysaccharides - chemistry</subject><subject>Polysaccharides - isolation & purification</subject><subject>SEC</subject><subject>Shear degradation</subject><subject>Structural characterization</subject><issn>0141-8130</issn><issn>1879-0003</issn><issn>1879-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhi0EokvhFSofuWSx46yTcKKqoEWqxAE4W5PJmPUqWQfbabt9D94XR2m5cvFY83__jEY_YxdSbKWQ-sNh6w6d8yPgthRltZWqKpV-wTayqdtCCKFeso2QlSwaqcQZexPjIXf1Tjav2ZlqRd3udLVhf767R-L0gMMcnT9y3Ic8NPlfAab9icOx5xBP40gpOISB28Hfc-to6Iv1GwBTNsLycOsDjynMmOaQYdzDIlNwj6vuLZ_8cIqAi-R6ih_5JUc_ThlM7o54oDtH92_ZKwtDpHdP9Zz9_PL5x9VNcfvt-uvV5W2BZbVLhe1AUWkFKrB1CRqhVja3OtsIrHfSElVSly0IqXpV913VgZa2Q9nYNpvUOXu_zp2C_z1TTGZ0EWkY4Eh-jkaJRqtGyFpmVK8oBh9jIGum4EYIJyOFWSIxB_MciVkiMWsk2XjxtGPuRur_2Z4zyMCnFaB8ab4-mIiOjki9C4TJ9N79b8df3KCmbw</recordid><startdate>202410</startdate><enddate>202410</enddate><creator>Chen, Zehong</creator><creator>Wang, Dan</creator><creator>Gu, Saisai</creator><creator>Wu, Niuniu</creator><creator>Wang, Kaiping</creator><creator>Zhang, Yu</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202410</creationdate><title>Size exclusion chromatography and asymmetrical flow field-flow fractionation for structural characterization of polysaccharides: A comparative review</title><author>Chen, Zehong ; Wang, Dan ; Gu, Saisai ; Wu, Niuniu ; Wang, Kaiping ; Zhang, Yu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c245t-fba3e2f0c3af72a6ca73fa3ebf80c751fee41629a013d37db4ba61fbc18f9c3a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Abnormal elution</topic><topic>AF4</topic><topic>Chromatography, Gel - methods</topic><topic>Fractionation, Field Flow - methods</topic><topic>Molecular Weight</topic><topic>Monosaccharides - analysis</topic><topic>Monosaccharides - chemistry</topic><topic>Polysaccharide</topic><topic>Polysaccharides - chemistry</topic><topic>Polysaccharides - isolation & purification</topic><topic>SEC</topic><topic>Shear degradation</topic><topic>Structural characterization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Zehong</creatorcontrib><creatorcontrib>Wang, Dan</creatorcontrib><creatorcontrib>Gu, Saisai</creatorcontrib><creatorcontrib>Wu, Niuniu</creatorcontrib><creatorcontrib>Wang, Kaiping</creatorcontrib><creatorcontrib>Zhang, Yu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of biological macromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Zehong</au><au>Wang, Dan</au><au>Gu, Saisai</au><au>Wu, Niuniu</au><au>Wang, Kaiping</au><au>Zhang, Yu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Size exclusion chromatography and asymmetrical flow field-flow fractionation for structural characterization of polysaccharides: A comparative review</atitle><jtitle>International journal of biological macromolecules</jtitle><addtitle>Int J Biol Macromol</addtitle><date>2024-10</date><risdate>2024</risdate><volume>277</volume><issue>Pt 2</issue><spage>134236</spage><pages>134236-</pages><artnum>134236</artnum><issn>0141-8130</issn><issn>1879-0003</issn><eissn>1879-0003</eissn><abstract>Natural polysaccharides exhibit a wide range of biological activities, which are closely related to their structural characteristics, including their molecular weight distribution, size, monosaccharide composition, glycosidic bond types and spatial conformation, etc. Size exclusion chromatography (SEC) and asymmetrical flow field-flow fractionation (AF4), as two potent separation techniques, both harbor potential for continuous development and enhancement. 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| subjects | Abnormal elution AF4 Chromatography, Gel - methods Fractionation, Field Flow - methods Molecular Weight Monosaccharides - analysis Monosaccharides - chemistry Polysaccharide Polysaccharides - chemistry Polysaccharides - isolation & purification SEC Shear degradation Structural characterization |
| title | Size exclusion chromatography and asymmetrical flow field-flow fractionation for structural characterization of polysaccharides: A comparative review |
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