An arginine-rich nuclear localization signal (ArgiNLS) strategy for streamlined image segmentation of single cells

High-throughput volumetric fluorescent microscopy pipelines can spatially integrate whole-brain structure and function at the foundational level of single cells. However, conventional fluorescent protein (FP) modifications used to discriminate single cells possess limited efficacy or are detrimental...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 2024-08, Vol.121 (32), p.e2320250121
Hauptverfasser:	Szelenyi, Eric R, Navarrete, Jovana S, Murry, Alexandria D, Zhang, Yizhe, Girven, Kasey S, Kuo, Lauren, Cline, Marcella M, Bernstein, Mollie X, Burdyniuk, Mariia, Bowler, Bryce, Goodwin, Nastacia L, Juarez, Barbara, Zweifel, Larry S, Golden, Sam A
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Arginine - metabolism Biological Sciences Brain - diagnostic imaging Brain - metabolism Cell Nucleus - metabolism Humans Image Processing, Computer-Assisted - methods Mice Microscopy, Fluorescence - methods Nuclear Localization Signals - metabolism Signal-To-Noise Ratio Single-Cell Analysis - methods
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!