Basal levels of biochemical biomarkers in the freshwater prawn Palaemon argentinus and their alterations due to the exposure of both insecticides cypermethrin and spirotetramat
The aim of this study was to evaluate the sensitivity of the prawn Palaemon argentinus to the pyrethroid cypermethrin (CYP) and the tetramic acid spirotetramat (STM). These treatments were compared with prawns collected at a reference site to define their basal physiological state. Initially, physic...
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| creator | Etcheverry, Leda Spaccesi, Fernando G. Cappelletti, Natalia E. Lavarías, Sabrina M.L. |
| description | The aim of this study was to evaluate the sensitivity of the prawn Palaemon argentinus to the pyrethroid cypermethrin (CYP) and the tetramic acid spirotetramat (STM). These treatments were compared with prawns collected at a reference site to define their basal physiological state. Initially, physicochemical parameters and several pollutants at the selected site were analyzed. The LC50-96 h was determined in adult prawns. Then, prawns were exposed for 96 h to sublethal concentrations of CYP (0.0005 μg/l) and STM (0.44 mg/l) to evaluate the effects on some biochemical endpoints. A treatment combining both pesticides was also added at 5 % of these values. Controls with and without solvent (acetone) were included. The LC50-96 h values were 0.005 μg/l and 4.43 mg/l for CYP and STM, respectively. Moreover, some biomarkers linked to oxidative and energy metabolism were analyzed in the hepatopancreas and muscle of both essayed prawns and those at the basal state. The STM caused a significant decrease in total protein content (32 %) in contrast to the increase of protein carbonyl content (71 %) (p |
| doi_str_mv | 10.1016/j.scitotenv.2024.174958 |
| format | Article |
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[Display omitted]
•The toxic effects of cypermethrin and spirotetramat were studied in P. argentinus.•The basal physiological state did not differ in the field and assayed prawns.•Prawns are very sensitive to environmental concentrations of cypermethrin.•Cypermethrin altered lipid homeostasis.•Spirotetramat was more harmful than cypermethrin at equitoxic concentrations.</description><identifier>ISSN: 0048-9697</identifier><identifier>ISSN: 1879-1026</identifier><identifier>EISSN: 1879-1026</identifier><identifier>DOI: 10.1016/j.scitotenv.2024.174958</identifier><identifier>PMID: 39067605</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Aquatic toxicology ; Crustacea ; Energy metabolism ; LC50 ; Oxidative stress ; Pesticides</subject><ispartof>The Science of the total environment, 2024-11, Vol.949, p.174958, Article 174958</ispartof><rights>2024 Elsevier B.V.</rights><rights>Copyright © 2024. Published by Elsevier B.V.</rights><rights>Copyright © 2024 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c247t-59d70f22627019d5d3c082413b5e06338d731eb14ac3bc2a78187df2435446a13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.scitotenv.2024.174958$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39067605$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Etcheverry, Leda</creatorcontrib><creatorcontrib>Spaccesi, Fernando G.</creatorcontrib><creatorcontrib>Cappelletti, Natalia E.</creatorcontrib><creatorcontrib>Lavarías, Sabrina M.L.</creatorcontrib><title>Basal levels of biochemical biomarkers in the freshwater prawn Palaemon argentinus and their alterations due to the exposure of both insecticides cypermethrin and spirotetramat</title><title>The Science of the total environment</title><addtitle>Sci Total Environ</addtitle><description>The aim of this study was to evaluate the sensitivity of the prawn Palaemon argentinus to the pyrethroid cypermethrin (CYP) and the tetramic acid spirotetramat (STM). These treatments were compared with prawns collected at a reference site to define their basal physiological state. Initially, physicochemical parameters and several pollutants at the selected site were analyzed. The LC50-96 h was determined in adult prawns. Then, prawns were exposed for 96 h to sublethal concentrations of CYP (0.0005 μg/l) and STM (0.44 mg/l) to evaluate the effects on some biochemical endpoints. A treatment combining both pesticides was also added at 5 % of these values. Controls with and without solvent (acetone) were included. The LC50-96 h values were 0.005 μg/l and 4.43 mg/l for CYP and STM, respectively. Moreover, some biomarkers linked to oxidative and energy metabolism were analyzed in the hepatopancreas and muscle of both essayed prawns and those at the basal state. The STM caused a significant decrease in total protein content (32 %) in contrast to the increase of protein carbonyl content (71 %) (p < 0.05). Also, glutathione S-transferase (52 %) and catalase (61 %) activities in the hepatopancreas of exposed prawns were higher compared to both the control and state basal groups (p < 0.05). In muscle, only a significant decrease in the lactate content (69 %) was caused by STM (p < 0.05). In addition, CYP caused a significant increase in the lactate dehydrogenase activity (110 %) in muscle and triacylglycerol content (73 %) in the hepatopancreas (p < 0.05). The integrated biomarker index (IBRv2) analysis showed that STM caused greater damage than CYP. Besides, the combined treatment showed an antagonistic interaction between both insecticides. The differential response of biomarkers to both CYP and STM exposure with respect to their basal levels shows a high sensitivity of P. argentinus demonstrating its potential role as a bioindicator organism.
[Display omitted]
•The toxic effects of cypermethrin and spirotetramat were studied in P. argentinus.•The basal physiological state did not differ in the field and assayed prawns.•Prawns are very sensitive to environmental concentrations of cypermethrin.•Cypermethrin altered lipid homeostasis.•Spirotetramat was more harmful than cypermethrin at equitoxic concentrations.</description><subject>Aquatic toxicology</subject><subject>Crustacea</subject><subject>Energy metabolism</subject><subject>LC50</subject><subject>Oxidative stress</subject><subject>Pesticides</subject><issn>0048-9697</issn><issn>1879-1026</issn><issn>1879-1026</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqFkc1u1DAUhSMEotPCK4CXbDL4J7GdZakKVKoEC1hbjn1DPCR2sJ0pfSseEadTusUbW9a55-ier6reErwnmPD3h30yLocM_rinmDZ7Ipqulc-qHZGiqwmm_Hm1w7iRdcc7cVadp3TA5QhJXlZnrMNccNzuqj8fdNITmuAIU0JhQL0LZoTZmfJb3rOOPyEm5DzKI6AhQhrvdIaIlqjvPPqqJw1z8EjHH-Cz82tC2ttN7CLSU1Hq7IJPyK6Acnhwgd9LSGuEh7yQx-KewGRnnIWEzP0CcYY8xhK6eaXFxbJqjnrW-VX1YtBTgteP90X1_eP1t6vP9e2XTzdXl7e1oY3IddtZgQdKORWYdLa1zGBJG8L6FjBnTFrBCPSk0Yb1hurSixR2oA1rm4Zrwi6qdyffJYZfK6SsZpcMTJP2ENakGJYtl03HZZGKk9TEkFKEQS3RleLuFcFqw6UO6gmX2nCpE64y-eYxZO1nsE9z__gUweVJUOjA0UHcjMAbsC6WxpQN7r8hfwGMgq9m</recordid><startdate>20241101</startdate><enddate>20241101</enddate><creator>Etcheverry, Leda</creator><creator>Spaccesi, Fernando G.</creator><creator>Cappelletti, Natalia E.</creator><creator>Lavarías, Sabrina M.L.</creator><general>Elsevier B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20241101</creationdate><title>Basal levels of biochemical biomarkers in the freshwater prawn Palaemon argentinus and their alterations due to the exposure of both insecticides cypermethrin and spirotetramat</title><author>Etcheverry, Leda ; Spaccesi, Fernando G. ; Cappelletti, Natalia E. ; Lavarías, Sabrina M.L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c247t-59d70f22627019d5d3c082413b5e06338d731eb14ac3bc2a78187df2435446a13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Aquatic toxicology</topic><topic>Crustacea</topic><topic>Energy metabolism</topic><topic>LC50</topic><topic>Oxidative stress</topic><topic>Pesticides</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Etcheverry, Leda</creatorcontrib><creatorcontrib>Spaccesi, Fernando G.</creatorcontrib><creatorcontrib>Cappelletti, Natalia E.</creatorcontrib><creatorcontrib>Lavarías, Sabrina M.L.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Science of the total environment</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Etcheverry, Leda</au><au>Spaccesi, Fernando G.</au><au>Cappelletti, Natalia E.</au><au>Lavarías, Sabrina M.L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Basal levels of biochemical biomarkers in the freshwater prawn Palaemon argentinus and their alterations due to the exposure of both insecticides cypermethrin and spirotetramat</atitle><jtitle>The Science of the total environment</jtitle><addtitle>Sci Total Environ</addtitle><date>2024-11-01</date><risdate>2024</risdate><volume>949</volume><spage>174958</spage><pages>174958-</pages><artnum>174958</artnum><issn>0048-9697</issn><issn>1879-1026</issn><eissn>1879-1026</eissn><abstract>The aim of this study was to evaluate the sensitivity of the prawn Palaemon argentinus to the pyrethroid cypermethrin (CYP) and the tetramic acid spirotetramat (STM). These treatments were compared with prawns collected at a reference site to define their basal physiological state. Initially, physicochemical parameters and several pollutants at the selected site were analyzed. The LC50-96 h was determined in adult prawns. Then, prawns were exposed for 96 h to sublethal concentrations of CYP (0.0005 μg/l) and STM (0.44 mg/l) to evaluate the effects on some biochemical endpoints. A treatment combining both pesticides was also added at 5 % of these values. Controls with and without solvent (acetone) were included. The LC50-96 h values were 0.005 μg/l and 4.43 mg/l for CYP and STM, respectively. Moreover, some biomarkers linked to oxidative and energy metabolism were analyzed in the hepatopancreas and muscle of both essayed prawns and those at the basal state. The STM caused a significant decrease in total protein content (32 %) in contrast to the increase of protein carbonyl content (71 %) (p < 0.05). Also, glutathione S-transferase (52 %) and catalase (61 %) activities in the hepatopancreas of exposed prawns were higher compared to both the control and state basal groups (p < 0.05). In muscle, only a significant decrease in the lactate content (69 %) was caused by STM (p < 0.05). In addition, CYP caused a significant increase in the lactate dehydrogenase activity (110 %) in muscle and triacylglycerol content (73 %) in the hepatopancreas (p < 0.05). The integrated biomarker index (IBRv2) analysis showed that STM caused greater damage than CYP. Besides, the combined treatment showed an antagonistic interaction between both insecticides. The differential response of biomarkers to both CYP and STM exposure with respect to their basal levels shows a high sensitivity of P. argentinus demonstrating its potential role as a bioindicator organism.
[Display omitted]
•The toxic effects of cypermethrin and spirotetramat were studied in P. argentinus.•The basal physiological state did not differ in the field and assayed prawns.•Prawns are very sensitive to environmental concentrations of cypermethrin.•Cypermethrin altered lipid homeostasis.•Spirotetramat was more harmful than cypermethrin at equitoxic concentrations.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>39067605</pmid><doi>10.1016/j.scitotenv.2024.174958</doi></addata></record> |
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| subjects | Aquatic toxicology Crustacea Energy metabolism LC50 Oxidative stress Pesticides |
| title | Basal levels of biochemical biomarkers in the freshwater prawn Palaemon argentinus and their alterations due to the exposure of both insecticides cypermethrin and spirotetramat |
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