Bidirectional hybridized hairpin DNA fluorescent aptasensor based on Au–Pd NPs and CDs for ratiometric detection of AFB1
A novel and simple ratiometric fluorescent aptasensor was developed for the sensitive detection of aflatoxin B1 (AFB1). A hairpin DNA (h-DNA) was independently synthesized as the basic skeleton, and the bidirectional hybridization of h-DNA can increase the load of aptamer and signal probes, thereby...
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| Veröffentlicht in: | Mikrochimica acta (1966) 2024-08, Vol.191 (8), p.489, Article 489 |
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| creator | Xu, Hongyan Zhao, Luyang Wan, Zhigang Liu, Yong Wei, Min |
| description | A novel and simple ratiometric fluorescent aptasensor was developed for the sensitive detection of aflatoxin B1 (AFB1). A hairpin DNA (h-DNA) was independently synthesized as the basic skeleton, and the bidirectional hybridization of h-DNA can increase the load of aptamer and signal probes, thereby realizing signal amplification. The high-efficiency fluorescence resonance energy transfer interaction between gold–palladium nanoparticles (Au–Pd NPs) and the self-synthesized fluorescent probe carbon dots (CDs) was utilized. Moreover, the label-free probe SYBR Green I (SG I) dye was introduced to form a double-signal probe with CDs, and a ratiometric sensor with F
CDs
/F
SG I
as a response signal was constructed. The ratio strategy can eliminate the fluctuation of external factors, thus improving the accuracy and reliability of the sensor. The quenching effect of Au–Pd NPs on CDs was 1.4 times that of AuNPs and 3.4 times that of Pd NPs, respectively. In the range 1–100 ng/mL, F
CDs
/F
SG I
showed a good linear relationship with the logarithm of the concentration of AFB1, and the limit of detection was as low as 0.07 ng/mL. The sensor was used to detect AFB1 in spiked peanuts and wine samples, and the recovery was between 91 and 115%, indicating that the sensor has high application potential in real sample analysis.
Graphical abstract |
| doi_str_mv | 10.1007/s00604-024-06560-5 |
| format | Article |
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CDs
/F
SG I
as a response signal was constructed. The ratio strategy can eliminate the fluctuation of external factors, thus improving the accuracy and reliability of the sensor. The quenching effect of Au–Pd NPs on CDs was 1.4 times that of AuNPs and 3.4 times that of Pd NPs, respectively. In the range 1–100 ng/mL, F
CDs
/F
SG I
showed a good linear relationship with the logarithm of the concentration of AFB1, and the limit of detection was as low as 0.07 ng/mL. The sensor was used to detect AFB1 in spiked peanuts and wine samples, and the recovery was between 91 and 115%, indicating that the sensor has high application potential in real sample analysis.
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CDs
/F
SG I
as a response signal was constructed. The ratio strategy can eliminate the fluctuation of external factors, thus improving the accuracy and reliability of the sensor. The quenching effect of Au–Pd NPs on CDs was 1.4 times that of AuNPs and 3.4 times that of Pd NPs, respectively. In the range 1–100 ng/mL, F
CDs
/F
SG I
showed a good linear relationship with the logarithm of the concentration of AFB1, and the limit of detection was as low as 0.07 ng/mL. The sensor was used to detect AFB1 in spiked peanuts and wine samples, and the recovery was between 91 and 115%, indicating that the sensor has high application potential in real sample analysis.
Graphical abstract</description><subject>Aflatoxin B1 - analysis</subject><subject>Aflatoxins</subject><subject>Analytical Chemistry</subject><subject>Aptamers, Nucleotide - chemistry</subject><subject>Arachis - chemistry</subject><subject>Biosensing Techniques - methods</subject><subject>Carbon - chemistry</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>DNA - chemistry</subject><subject>Energy transfer</subject><subject>Fluorescence Resonance Energy Transfer - methods</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Fluorescent indicators</subject><subject>Gold</subject><subject>Gold - chemistry</subject><subject>Inverted Repeat Sequences</subject><subject>Limit of Detection</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Microengineering</subject><subject>Nanochemistry</subject><subject>Nanoparticles</subject><subject>Nanotechnology</subject><subject>Nucleic Acid Hybridization</subject><subject>Original Paper</subject><subject>Palladium</subject><subject>Palladium - chemistry</subject><subject>Quantum Dots - chemistry</subject><subject>Sensors</subject><subject>Synthesis</subject><subject>Wine - analysis</subject><issn>0026-3672</issn><issn>1436-5073</issn><issn>1436-5073</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kbtuFTEQhi0EIofAC1AgSzQ0C-PresuTEwJIUUgBteXLLNloz_pg7xZJxTvwhjwJDhtAoqCwbI2_-Uf2R8hzBq8ZQPumAGiQDfC6tNLQqAdkw6TQjYJWPCQbAK4boVt-RJ6Ucg3AWs3lY3IkOtC6E2ZDbk-GOGQM85AmN9KrG59r4RYjvXJDPgwTPb3Y0n5cUsYScJqpO8yu4FRSpr4eIk0T3S4_vn2_jPTislA3Rbo7LbSvQHY1d49zHgKNOK9jaOrp9uyEPSWPejcWfHa_H5PPZ28_7d435x_ffdhtz5vAlZ6bELQXMqouGN-qjsvWg--V1Co4gM4HHpTRTqIERO1ZNGgQOaDrOdOsF8fk1Zp7yOnrgmW2-6E-ZRzdhGkpVoBR2kijeEVf_oNepyXXj1kpzpUxdxRfqZBTKRl7e8jD3uUby8DembGrGVvN2F9mrKpNL-6jF7_H-Kflt4oKiBUo9Wr6gvnv7P_E_gR2upnl</recordid><startdate>20240801</startdate><enddate>20240801</enddate><creator>Xu, Hongyan</creator><creator>Zhao, Luyang</creator><creator>Wan, Zhigang</creator><creator>Liu, Yong</creator><creator>Wei, Min</creator><general>Springer Vienna</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-2966-5041</orcidid></search><sort><creationdate>20240801</creationdate><title>Bidirectional hybridized hairpin DNA fluorescent aptasensor based on Au–Pd NPs and CDs for ratiometric detection of AFB1</title><author>Xu, Hongyan ; Zhao, Luyang ; Wan, Zhigang ; Liu, Yong ; Wei, Min</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c256t-cc6b34d59c8b759247b0bf5465ca009bc2c586a4e40ee6b1d8e8ee20eaf2161f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Aflatoxin B1 - analysis</topic><topic>Aflatoxins</topic><topic>Analytical Chemistry</topic><topic>Aptamers, Nucleotide - chemistry</topic><topic>Arachis - chemistry</topic><topic>Biosensing Techniques - methods</topic><topic>Carbon - chemistry</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>DNA - chemistry</topic><topic>Energy transfer</topic><topic>Fluorescence Resonance Energy Transfer - methods</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Fluorescent indicators</topic><topic>Gold</topic><topic>Gold - chemistry</topic><topic>Inverted Repeat Sequences</topic><topic>Limit of Detection</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Microengineering</topic><topic>Nanochemistry</topic><topic>Nanoparticles</topic><topic>Nanotechnology</topic><topic>Nucleic Acid Hybridization</topic><topic>Original Paper</topic><topic>Palladium</topic><topic>Palladium - chemistry</topic><topic>Quantum Dots - chemistry</topic><topic>Sensors</topic><topic>Synthesis</topic><topic>Wine - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xu, Hongyan</creatorcontrib><creatorcontrib>Zhao, Luyang</creatorcontrib><creatorcontrib>Wan, Zhigang</creatorcontrib><creatorcontrib>Liu, Yong</creatorcontrib><creatorcontrib>Wei, Min</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Mikrochimica acta (1966)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, Hongyan</au><au>Zhao, Luyang</au><au>Wan, Zhigang</au><au>Liu, Yong</au><au>Wei, Min</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bidirectional hybridized hairpin DNA fluorescent aptasensor based on Au–Pd NPs and CDs for ratiometric detection of AFB1</atitle><jtitle>Mikrochimica acta (1966)</jtitle><stitle>Microchim Acta</stitle><addtitle>Mikrochim Acta</addtitle><date>2024-08-01</date><risdate>2024</risdate><volume>191</volume><issue>8</issue><spage>489</spage><pages>489-</pages><artnum>489</artnum><issn>0026-3672</issn><issn>1436-5073</issn><eissn>1436-5073</eissn><abstract>A novel and simple ratiometric fluorescent aptasensor was developed for the sensitive detection of aflatoxin B1 (AFB1). A hairpin DNA (h-DNA) was independently synthesized as the basic skeleton, and the bidirectional hybridization of h-DNA can increase the load of aptamer and signal probes, thereby realizing signal amplification. The high-efficiency fluorescence resonance energy transfer interaction between gold–palladium nanoparticles (Au–Pd NPs) and the self-synthesized fluorescent probe carbon dots (CDs) was utilized. Moreover, the label-free probe SYBR Green I (SG I) dye was introduced to form a double-signal probe with CDs, and a ratiometric sensor with F
CDs
/F
SG I
as a response signal was constructed. The ratio strategy can eliminate the fluctuation of external factors, thus improving the accuracy and reliability of the sensor. The quenching effect of Au–Pd NPs on CDs was 1.4 times that of AuNPs and 3.4 times that of Pd NPs, respectively. In the range 1–100 ng/mL, F
CDs
/F
SG I
showed a good linear relationship with the logarithm of the concentration of AFB1, and the limit of detection was as low as 0.07 ng/mL. The sensor was used to detect AFB1 in spiked peanuts and wine samples, and the recovery was between 91 and 115%, indicating that the sensor has high application potential in real sample analysis.
Graphical abstract</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><pmid>39066938</pmid><doi>10.1007/s00604-024-06560-5</doi><orcidid>https://orcid.org/0000-0003-2966-5041</orcidid></addata></record> |
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| ispartof | Mikrochimica acta (1966), 2024-08, Vol.191 (8), p.489, Article 489 |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Aflatoxin B1 - analysis Aflatoxins Analytical Chemistry Aptamers, Nucleotide - chemistry Arachis - chemistry Biosensing Techniques - methods Carbon - chemistry Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science DNA - chemistry Energy transfer Fluorescence Resonance Energy Transfer - methods Fluorescent Dyes - chemistry Fluorescent indicators Gold Gold - chemistry Inverted Repeat Sequences Limit of Detection Metal Nanoparticles - chemistry Microengineering Nanochemistry Nanoparticles Nanotechnology Nucleic Acid Hybridization Original Paper Palladium Palladium - chemistry Quantum Dots - chemistry Sensors Synthesis Wine - analysis |
| title | Bidirectional hybridized hairpin DNA fluorescent aptasensor based on Au–Pd NPs and CDs for ratiometric detection of AFB1 |
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