Whole proteome analysis of germinating and outgrowing Bacillus subtilis 168
In this study, we present a high‐resolution dataset and bioinformatic analysis of the proteome of Bacillus subtilis 168 trp+ (BSB1) during germination and spore outgrowth. Samples were collected at 14 different time points (ranging from 0 to 130 min) in three biological replicates after spore inocul...
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| Veröffentlicht in: | Proteomics (Weinheim) 2024-09, Vol.24 (17), p.e2400031-n/a |
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| creator | Pospíšil, Jiří Sax, Alice Hubálek, Martin Krásný, Libor Vohradský, Jiří |
| description | In this study, we present a high‐resolution dataset and bioinformatic analysis of the proteome of Bacillus subtilis 168 trp+ (BSB1) during germination and spore outgrowth. Samples were collected at 14 different time points (ranging from 0 to 130 min) in three biological replicates after spore inoculation into germination medium. A total of 2191 proteins were identified and categorized based on their expression kinetics. We observed four distinct clusters that were analyzed for functional categories and KEGG pathways annotations. The examination of newly synthesized proteins between successive time points revealed significant changes, particularly within the first 50 min. The dataset provides an information base that can be used for modeling purposes and inspire the design of new experiments. |
| doi_str_mv | 10.1002/pmic.202400031 |
| format | Article |
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| subjects | Annotations Bacillus subtilis Bacillus subtilis - growth & development Bacillus subtilis - metabolism Bacterial Proteins - metabolism Cluster analysis Computational Biology - methods Datasets Germination Inoculation KEGG pathways analysis protein functional analysis Proteins Proteome - analysis Proteome - metabolism Proteomes Proteomics - methods Spore outgrowth Spores, Bacterial - growth & development Spores, Bacterial - metabolism time series of protein expression |
| title | Whole proteome analysis of germinating and outgrowing Bacillus subtilis 168 |
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