Evaluation and comparison of liquid chromatography/high‐resolution mass spectrometry platforms for the separation and characterization of ginsenosides from the leaves of Panax ginseng
The measurement of data repeatability in small‐molecule metabolites acquired within and among different liquid chromatography‐mass spectrometry (LC‐MS) platforms is crucial for data sharing or data transfer in natural products research. This work was designed to investigate and evaluate the separati...
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| Veröffentlicht in: | Journal of separation science 2024-07, Vol.47 (14), p.e2400354-n/a |
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| creator | Li, Xiaohang Zou, Yadan Cheng, Huizhen Ding, Mengxiang Yang, Yang Hong, Lili Xiong, Ying Zhang, Min Li, Xue Chen, Qinhua Wang, Hongda Cui, Yuanwu Yang, Wenzhi |
| description | The measurement of data repeatability in small‐molecule metabolites acquired within and among different liquid chromatography‐mass spectrometry (LC‐MS) platforms is crucial for data sharing or data transfer in natural products research. This work was designed to investigate and evaluate the separation and detection performance of three commercial high‐resolution LC‐MS platforms (e.g., Agilent 6550 QTOF, Waters Vion IM‐QTOF, and Thermo Scientific Orbitrap Exploris 120) using 68 ginsenoside references and the extract of Panax ginseng leaf. The retention time (tR), measured on these three platforms (under the same chromatography condition), showed good stability in different concentration tests, and within/among different instruments for both intra‐day and inter‐day precision examinations. Correlation in tR of ginsenosides was also highly determined on these three platforms. In spite of the different mass analyzers involved, these three platforms gave the accurate mass determination ability, especially enhanced resolution gained because of the ion mobility (IM) separation facilitated by IM‐quadrupole time‐of‐flight. The current study has systematically evaluated the separation and MS detection performance enabled by three high‐resolution LC‐MS platforms taking ginsenosides as the template, and the reported findings can benefit the researchers for the selection of analytical platforms and the purpose of data sharing or data transfer. |
| doi_str_mv | 10.1002/jssc.202400354 |
| format | Article |
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This work was designed to investigate and evaluate the separation and detection performance of three commercial high‐resolution LC‐MS platforms (e.g., Agilent 6550 QTOF, Waters Vion IM‐QTOF, and Thermo Scientific Orbitrap Exploris 120) using 68 ginsenoside references and the extract of Panax ginseng leaf. The retention time (tR), measured on these three platforms (under the same chromatography condition), showed good stability in different concentration tests, and within/among different instruments for both intra‐day and inter‐day precision examinations. Correlation in tR of ginsenosides was also highly determined on these three platforms. In spite of the different mass analyzers involved, these three platforms gave the accurate mass determination ability, especially enhanced resolution gained because of the ion mobility (IM) separation facilitated by IM‐quadrupole time‐of‐flight. The current study has systematically evaluated the separation and MS detection performance enabled by three high‐resolution LC‐MS platforms taking ginsenosides as the template, and the reported findings can benefit the researchers for the selection of analytical platforms and the purpose of data sharing or data transfer.</description><identifier>ISSN: 1615-9306</identifier><identifier>ISSN: 1615-9314</identifier><identifier>EISSN: 1615-9314</identifier><identifier>DOI: 10.1002/jssc.202400354</identifier><identifier>PMID: 39034839</identifier><language>eng</language><publisher>Germany: Wiley Subscription Services, Inc</publisher><subject>Analyzers ; Chromatography ; Chromatography, High Pressure Liquid - methods ; Chromatography, Liquid - methods ; Data acquisition ; Data analysis ; Data transfer (computers) ; Extraction processes ; ginsenoside ; Ginsenosides - analysis ; Ginsenosides - chemistry ; Ginsenosides - isolation & purification ; Herbal medicine ; Information sharing ; Ionic mobility ; Ions ; Liquid chromatography ; liquid chromatography/mass spectrometry ; Mass Spectrometry ; Metabolites ; Natural products ; Panax - chemistry ; Performance evaluation ; Plant Leaves - chemistry ; Platforms ; Quadrupoles ; reproducibility ; retention time ; Scientific imaging ; Separation ; stability ; Time measurement</subject><ispartof>Journal of separation science, 2024-07, Vol.47 (14), p.e2400354-n/a</ispartof><rights>2024 Wiley‐VCH GmbH.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c2539-3d67862debf893c847768f5b87a09ecbdc04dac281f66d900983f52052c2d9fe3</cites><orcidid>0000-0002-9108-1314 ; 0000-0003-2780-4853</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjssc.202400354$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjssc.202400354$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39034839$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Xiaohang</creatorcontrib><creatorcontrib>Zou, Yadan</creatorcontrib><creatorcontrib>Cheng, Huizhen</creatorcontrib><creatorcontrib>Ding, Mengxiang</creatorcontrib><creatorcontrib>Yang, Yang</creatorcontrib><creatorcontrib>Hong, Lili</creatorcontrib><creatorcontrib>Xiong, Ying</creatorcontrib><creatorcontrib>Zhang, Min</creatorcontrib><creatorcontrib>Li, Xue</creatorcontrib><creatorcontrib>Chen, Qinhua</creatorcontrib><creatorcontrib>Wang, Hongda</creatorcontrib><creatorcontrib>Cui, Yuanwu</creatorcontrib><creatorcontrib>Yang, Wenzhi</creatorcontrib><title>Evaluation and comparison of liquid chromatography/high‐resolution mass spectrometry platforms for the separation and characterization of ginsenosides from the leaves of Panax ginseng</title><title>Journal of separation science</title><addtitle>J Sep Sci</addtitle><description>The measurement of data repeatability in small‐molecule metabolites acquired within and among different liquid chromatography‐mass spectrometry (LC‐MS) platforms is crucial for data sharing or data transfer in natural products research. This work was designed to investigate and evaluate the separation and detection performance of three commercial high‐resolution LC‐MS platforms (e.g., Agilent 6550 QTOF, Waters Vion IM‐QTOF, and Thermo Scientific Orbitrap Exploris 120) using 68 ginsenoside references and the extract of Panax ginseng leaf. The retention time (tR), measured on these three platforms (under the same chromatography condition), showed good stability in different concentration tests, and within/among different instruments for both intra‐day and inter‐day precision examinations. Correlation in tR of ginsenosides was also highly determined on these three platforms. In spite of the different mass analyzers involved, these three platforms gave the accurate mass determination ability, especially enhanced resolution gained because of the ion mobility (IM) separation facilitated by IM‐quadrupole time‐of‐flight. The current study has systematically evaluated the separation and MS detection performance enabled by three high‐resolution LC‐MS platforms taking ginsenosides as the template, and the reported findings can benefit the researchers for the selection of analytical platforms and the purpose of data sharing or data transfer.</description><subject>Analyzers</subject><subject>Chromatography</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Chromatography, Liquid - methods</subject><subject>Data acquisition</subject><subject>Data analysis</subject><subject>Data transfer (computers)</subject><subject>Extraction processes</subject><subject>ginsenoside</subject><subject>Ginsenosides - analysis</subject><subject>Ginsenosides - chemistry</subject><subject>Ginsenosides - isolation & purification</subject><subject>Herbal medicine</subject><subject>Information sharing</subject><subject>Ionic mobility</subject><subject>Ions</subject><subject>Liquid chromatography</subject><subject>liquid chromatography/mass spectrometry</subject><subject>Mass Spectrometry</subject><subject>Metabolites</subject><subject>Natural products</subject><subject>Panax - chemistry</subject><subject>Performance evaluation</subject><subject>Plant Leaves - chemistry</subject><subject>Platforms</subject><subject>Quadrupoles</subject><subject>reproducibility</subject><subject>retention time</subject><subject>Scientific imaging</subject><subject>Separation</subject><subject>stability</subject><subject>Time measurement</subject><issn>1615-9306</issn><issn>1615-9314</issn><issn>1615-9314</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9u1DAQxiMEoqVw5Ygicellt2M7f-wjWpU_VSUqFc6R1xlvvEri1JO0bE88Aq_D6_Ak9XaXVcWFi-0Z_75vRvqS5C2DOQPgZ2siM-fAMwCRZ8-SY1awfKYEy54f3lAcJa-I1gCslApeJkdCgcikUMfJ7_Nb3U56dL5PdV-nxneDDo5i6W3aupvJxWYTfKdHvwp6aDZnjVs1f37-Cki-nR6VnSZKaUAzRhDHsEmHVo_Wh47SeKZjgylhNH4yqImVGTG4-10zjlu5nrD35GqMumj1KGxR38Y6_l_pXv_YU6vXyQurW8I3-_sk-f7x_Nvi8-zy66cviw-XM8NzoWaiLkpZ8BqXViphZFaWhbT5UpYaFJplbSCrteGS2aKoFYCSwuYccm54rSyKk-R05zsEfzMhjVXnyGDb6h79RJUAKTgrmWARff8PuvZT6ON2WyrPGXDJIzXfUSZ4ooC2GoLrdNhUDKptqNU21OoQahS829tOyw7rA_43xQhkO-DOtbj5j111cX29kAKUeAAGWLPR</recordid><startdate>202407</startdate><enddate>202407</enddate><creator>Li, Xiaohang</creator><creator>Zou, Yadan</creator><creator>Cheng, Huizhen</creator><creator>Ding, Mengxiang</creator><creator>Yang, Yang</creator><creator>Hong, Lili</creator><creator>Xiong, Ying</creator><creator>Zhang, Min</creator><creator>Li, Xue</creator><creator>Chen, Qinhua</creator><creator>Wang, Hongda</creator><creator>Cui, Yuanwu</creator><creator>Yang, Wenzhi</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-9108-1314</orcidid><orcidid>https://orcid.org/0000-0003-2780-4853</orcidid></search><sort><creationdate>202407</creationdate><title>Evaluation and comparison of liquid chromatography/high‐resolution mass spectrometry platforms for the separation and characterization of ginsenosides from the leaves of Panax ginseng</title><author>Li, Xiaohang ; Zou, Yadan ; Cheng, Huizhen ; Ding, Mengxiang ; Yang, Yang ; Hong, Lili ; Xiong, Ying ; Zhang, Min ; Li, Xue ; Chen, Qinhua ; Wang, Hongda ; Cui, Yuanwu ; Yang, Wenzhi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2539-3d67862debf893c847768f5b87a09ecbdc04dac281f66d900983f52052c2d9fe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Analyzers</topic><topic>Chromatography</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Chromatography, Liquid - methods</topic><topic>Data acquisition</topic><topic>Data analysis</topic><topic>Data transfer (computers)</topic><topic>Extraction processes</topic><topic>ginsenoside</topic><topic>Ginsenosides - analysis</topic><topic>Ginsenosides - chemistry</topic><topic>Ginsenosides - isolation & purification</topic><topic>Herbal medicine</topic><topic>Information sharing</topic><topic>Ionic mobility</topic><topic>Ions</topic><topic>Liquid chromatography</topic><topic>liquid chromatography/mass spectrometry</topic><topic>Mass Spectrometry</topic><topic>Metabolites</topic><topic>Natural products</topic><topic>Panax - chemistry</topic><topic>Performance evaluation</topic><topic>Plant Leaves - chemistry</topic><topic>Platforms</topic><topic>Quadrupoles</topic><topic>reproducibility</topic><topic>retention time</topic><topic>Scientific imaging</topic><topic>Separation</topic><topic>stability</topic><topic>Time measurement</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Xiaohang</creatorcontrib><creatorcontrib>Zou, Yadan</creatorcontrib><creatorcontrib>Cheng, Huizhen</creatorcontrib><creatorcontrib>Ding, Mengxiang</creatorcontrib><creatorcontrib>Yang, Yang</creatorcontrib><creatorcontrib>Hong, Lili</creatorcontrib><creatorcontrib>Xiong, Ying</creatorcontrib><creatorcontrib>Zhang, Min</creatorcontrib><creatorcontrib>Li, Xue</creatorcontrib><creatorcontrib>Chen, Qinhua</creatorcontrib><creatorcontrib>Wang, Hongda</creatorcontrib><creatorcontrib>Cui, Yuanwu</creatorcontrib><creatorcontrib>Yang, Wenzhi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of separation science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Xiaohang</au><au>Zou, Yadan</au><au>Cheng, Huizhen</au><au>Ding, Mengxiang</au><au>Yang, Yang</au><au>Hong, Lili</au><au>Xiong, Ying</au><au>Zhang, Min</au><au>Li, Xue</au><au>Chen, Qinhua</au><au>Wang, Hongda</au><au>Cui, Yuanwu</au><au>Yang, Wenzhi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation and comparison of liquid chromatography/high‐resolution mass spectrometry platforms for the separation and characterization of ginsenosides from the leaves of Panax ginseng</atitle><jtitle>Journal of separation science</jtitle><addtitle>J Sep Sci</addtitle><date>2024-07</date><risdate>2024</risdate><volume>47</volume><issue>14</issue><spage>e2400354</spage><epage>n/a</epage><pages>e2400354-n/a</pages><issn>1615-9306</issn><issn>1615-9314</issn><eissn>1615-9314</eissn><abstract>The measurement of data repeatability in small‐molecule metabolites acquired within and among different liquid chromatography‐mass spectrometry (LC‐MS) platforms is crucial for data sharing or data transfer in natural products research. This work was designed to investigate and evaluate the separation and detection performance of three commercial high‐resolution LC‐MS platforms (e.g., Agilent 6550 QTOF, Waters Vion IM‐QTOF, and Thermo Scientific Orbitrap Exploris 120) using 68 ginsenoside references and the extract of Panax ginseng leaf. The retention time (tR), measured on these three platforms (under the same chromatography condition), showed good stability in different concentration tests, and within/among different instruments for both intra‐day and inter‐day precision examinations. Correlation in tR of ginsenosides was also highly determined on these three platforms. In spite of the different mass analyzers involved, these three platforms gave the accurate mass determination ability, especially enhanced resolution gained because of the ion mobility (IM) separation facilitated by IM‐quadrupole time‐of‐flight. The current study has systematically evaluated the separation and MS detection performance enabled by three high‐resolution LC‐MS platforms taking ginsenosides as the template, and the reported findings can benefit the researchers for the selection of analytical platforms and the purpose of data sharing or data transfer.</abstract><cop>Germany</cop><pub>Wiley Subscription Services, Inc</pub><pmid>39034839</pmid><doi>10.1002/jssc.202400354</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-9108-1314</orcidid><orcidid>https://orcid.org/0000-0003-2780-4853</orcidid></addata></record> |
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| subjects | Analyzers Chromatography Chromatography, High Pressure Liquid - methods Chromatography, Liquid - methods Data acquisition Data analysis Data transfer (computers) Extraction processes ginsenoside Ginsenosides - analysis Ginsenosides - chemistry Ginsenosides - isolation & purification Herbal medicine Information sharing Ionic mobility Ions Liquid chromatography liquid chromatography/mass spectrometry Mass Spectrometry Metabolites Natural products Panax - chemistry Performance evaluation Plant Leaves - chemistry Platforms Quadrupoles reproducibility retention time Scientific imaging Separation stability Time measurement |
| title | Evaluation and comparison of liquid chromatography/high‐resolution mass spectrometry platforms for the separation and characterization of ginsenosides from the leaves of Panax ginseng |
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