Dispersive solid-phase extraction based on zirconium metal-organic framework coupled with gas chromatography-mass spectrometry for determining sugar phosphates in biological samples
Sugar phosphates (SPx) play important role in the metabolism of the organism. SPx such as glycerate 3-phosphate, fructose 6-phosphate and glucose 6-phosphate in biological samples have the poor stability, similar structure and low abundance, which make their separation and detection more challenging...
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| Veröffentlicht in: | Analytica chimica acta 2024-08, Vol.1317, p.342908, Article 342908 |
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| description | Sugar phosphates (SPx) play important role in the metabolism of the organism. SPx such as glycerate 3-phosphate, fructose 6-phosphate and glucose 6-phosphate in biological samples have the poor stability, similar structure and low abundance, which make their separation and detection more challenging.
UiO-66-NH2 and ZrO2 coated SiO2(SBA-15) hard-core–shell adsorbents (UiO-66-NH2@SBA-15 and ZrO2@SBA-15) were synthesized, which were further used for dispersive solid-phase extraction for enriching the SPx in biological samples. The protocol was developed by UiO-66-NH2@SBA-15 and ZrO2@SBA-15 coupled with gas chromatography-mass spectrometry for the detection of trace SPx. The univariate experiment and response surface methodology were used to optimize the adsorption and desorption conditions.
The adsorbents showed excellent adsorption capacity and specificity towards SPx, which were proved by adsorption and selective experiments. Under the optimized conditions, there were good linearity within the range of 5.0–5000.0 ng mL−1, low limits of detection (0.001–1.0 ng mL−1), low limits of quantification (0.005–5.0 ng mL−1) and good precision (relative standard deviation less than 14.7 % for intra-day and inter-day). The satisfactory recoveries (89.1–113.8 %) and precision (0.5–14.6 %) were obtained when the sorbents were used to extract SPx from serum, saliva and cell samples. Moreover, UiO-66-NH2@SBA-15 was applied to the quantitative analysis of SPx from gastric cancer patients, because of a higher adsorption capacity (169.5–196.1 mg g−1).
UiO-66-NH2@SBA-15 showed great potential in the extraction of SPx in biological samples, which was beneficial to find out the metabolic change of SPx and explain the pathogenesis of the disease.
[Display omitted]
•UiO-66-NH2@SBA-15 and ZrO2@SBA-15 demonstrated specific adsorption towards sugar phosphates.•An efficient dispersive solid phase extraction protocol was developed for sugar phosphates in the biological samples.•UiO-66-NH2@SBA-15 exhibited the highest adsorption capacity. |
| doi_str_mv | 10.1016/j.aca.2024.342908 |
| format | Article |
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UiO-66-NH2 and ZrO2 coated SiO2(SBA-15) hard-core–shell adsorbents (UiO-66-NH2@SBA-15 and ZrO2@SBA-15) were synthesized, which were further used for dispersive solid-phase extraction for enriching the SPx in biological samples. The protocol was developed by UiO-66-NH2@SBA-15 and ZrO2@SBA-15 coupled with gas chromatography-mass spectrometry for the detection of trace SPx. The univariate experiment and response surface methodology were used to optimize the adsorption and desorption conditions.
The adsorbents showed excellent adsorption capacity and specificity towards SPx, which were proved by adsorption and selective experiments. Under the optimized conditions, there were good linearity within the range of 5.0–5000.0 ng mL−1, low limits of detection (0.001–1.0 ng mL−1), low limits of quantification (0.005–5.0 ng mL−1) and good precision (relative standard deviation less than 14.7 % for intra-day and inter-day). The satisfactory recoveries (89.1–113.8 %) and precision (0.5–14.6 %) were obtained when the sorbents were used to extract SPx from serum, saliva and cell samples. Moreover, UiO-66-NH2@SBA-15 was applied to the quantitative analysis of SPx from gastric cancer patients, because of a higher adsorption capacity (169.5–196.1 mg g−1).
UiO-66-NH2@SBA-15 showed great potential in the extraction of SPx in biological samples, which was beneficial to find out the metabolic change of SPx and explain the pathogenesis of the disease.
[Display omitted]
•UiO-66-NH2@SBA-15 and ZrO2@SBA-15 demonstrated specific adsorption towards sugar phosphates.•An efficient dispersive solid phase extraction protocol was developed for sugar phosphates in the biological samples.•UiO-66-NH2@SBA-15 exhibited the highest adsorption capacity.</description><identifier>ISSN: 0003-2670</identifier><identifier>ISSN: 1873-4324</identifier><identifier>EISSN: 1873-4324</identifier><identifier>DOI: 10.1016/j.aca.2024.342908</identifier><identifier>PMID: 39030009</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Adsorption ; Biological samples ; Dispersive solid-phase extraction ; Gas Chromatography-Mass Spectrometry - methods ; Humans ; Limit of Detection ; Metal-organic frameworks ; Metal-Organic Frameworks - chemistry ; Phosphates - chemistry ; Phthalic Acids ; Silicon Dioxide - chemistry ; Solid Phase Extraction - methods ; Sugar phosphates ; Zirconium - chemistry ; ZrO2</subject><ispartof>Analytica chimica acta, 2024-08, Vol.1317, p.342908, Article 342908</ispartof><rights>2024 Elsevier B.V.</rights><rights>Copyright © 2024 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c235t-2f206fb538e7d0194d714b68a6e8159ab70ed9a63cb346618fd97fee4e4f63d53</cites><orcidid>0000-0001-6766-9508</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.aca.2024.342908$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39030009$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hu, Yuyan</creatorcontrib><creatorcontrib>Wu, Boxue</creatorcontrib><creatorcontrib>Tang, Ying-Shu</creatorcontrib><creatorcontrib>Wu, Yi</creatorcontrib><creatorcontrib>Liu, Li-Yan</creatorcontrib><title>Dispersive solid-phase extraction based on zirconium metal-organic framework coupled with gas chromatography-mass spectrometry for determining sugar phosphates in biological samples</title><title>Analytica chimica acta</title><addtitle>Anal Chim Acta</addtitle><description>Sugar phosphates (SPx) play important role in the metabolism of the organism. SPx such as glycerate 3-phosphate, fructose 6-phosphate and glucose 6-phosphate in biological samples have the poor stability, similar structure and low abundance, which make their separation and detection more challenging.
UiO-66-NH2 and ZrO2 coated SiO2(SBA-15) hard-core–shell adsorbents (UiO-66-NH2@SBA-15 and ZrO2@SBA-15) were synthesized, which were further used for dispersive solid-phase extraction for enriching the SPx in biological samples. The protocol was developed by UiO-66-NH2@SBA-15 and ZrO2@SBA-15 coupled with gas chromatography-mass spectrometry for the detection of trace SPx. The univariate experiment and response surface methodology were used to optimize the adsorption and desorption conditions.
The adsorbents showed excellent adsorption capacity and specificity towards SPx, which were proved by adsorption and selective experiments. Under the optimized conditions, there were good linearity within the range of 5.0–5000.0 ng mL−1, low limits of detection (0.001–1.0 ng mL−1), low limits of quantification (0.005–5.0 ng mL−1) and good precision (relative standard deviation less than 14.7 % for intra-day and inter-day). The satisfactory recoveries (89.1–113.8 %) and precision (0.5–14.6 %) were obtained when the sorbents were used to extract SPx from serum, saliva and cell samples. Moreover, UiO-66-NH2@SBA-15 was applied to the quantitative analysis of SPx from gastric cancer patients, because of a higher adsorption capacity (169.5–196.1 mg g−1).
UiO-66-NH2@SBA-15 showed great potential in the extraction of SPx in biological samples, which was beneficial to find out the metabolic change of SPx and explain the pathogenesis of the disease.
[Display omitted]
•UiO-66-NH2@SBA-15 and ZrO2@SBA-15 demonstrated specific adsorption towards sugar phosphates.•An efficient dispersive solid phase extraction protocol was developed for sugar phosphates in the biological samples.•UiO-66-NH2@SBA-15 exhibited the highest adsorption capacity.</description><subject>Adsorption</subject><subject>Biological samples</subject><subject>Dispersive solid-phase extraction</subject><subject>Gas Chromatography-Mass Spectrometry - methods</subject><subject>Humans</subject><subject>Limit of Detection</subject><subject>Metal-organic frameworks</subject><subject>Metal-Organic Frameworks - chemistry</subject><subject>Phosphates - chemistry</subject><subject>Phthalic Acids</subject><subject>Silicon Dioxide - chemistry</subject><subject>Solid Phase Extraction - methods</subject><subject>Sugar phosphates</subject><subject>Zirconium - chemistry</subject><subject>ZrO2</subject><issn>0003-2670</issn><issn>1873-4324</issn><issn>1873-4324</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcuO1DAQRS0EYpqBD2CDvGSTxq_OQ6zQDC9pJDawthy7kriJ4-ByZmj-i__Dox5YsrJv6datsg8hLznbc8brN8e9sWYvmFB7qUTH2kdkx9tGVkoK9ZjsGGOyEnXDLsgzxGORgjP1lFzIjsmiuh35fe1xhYT-FijG2btqnQwChZ85GZt9XGhftKPl8ssnGxe_BRogm7mKaTSLt3RIJsBdTN-pjds6F_OdzxMdDVI7pRhMjmMy63SqgkGkZZ7NpQw5negQE3WQIQW_-GWkuI0m0XWKWPbIgNSXBXyc4-itmSmaUAbgc_JkMDPCi4fzknz78P7r1afq5svHz1fvbior5CFXYhCsHvqDbKFxjHfKNVz1dWtqaPmhM33DwHWmlraXqq55O7iuGQAUqKGW7iAvyetz7prijw0w6-DRwjybBeKGWrJWtFLJmhcrP1ttiogJBr0mH0w6ac70PS191IWWvqelz7RKz6uH-K0P4P51_MVTDG_PBiiPvPWQNFoPiwXnU_lE7aL_T_wfT6Wq_g</recordid><startdate>20240815</startdate><enddate>20240815</enddate><creator>Hu, Yuyan</creator><creator>Wu, Boxue</creator><creator>Tang, Ying-Shu</creator><creator>Wu, Yi</creator><creator>Liu, Li-Yan</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6766-9508</orcidid></search><sort><creationdate>20240815</creationdate><title>Dispersive solid-phase extraction based on zirconium metal-organic framework coupled with gas chromatography-mass spectrometry for determining sugar phosphates in biological samples</title><author>Hu, Yuyan ; Wu, Boxue ; Tang, Ying-Shu ; Wu, Yi ; Liu, Li-Yan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c235t-2f206fb538e7d0194d714b68a6e8159ab70ed9a63cb346618fd97fee4e4f63d53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Adsorption</topic><topic>Biological samples</topic><topic>Dispersive solid-phase extraction</topic><topic>Gas Chromatography-Mass Spectrometry - methods</topic><topic>Humans</topic><topic>Limit of Detection</topic><topic>Metal-organic frameworks</topic><topic>Metal-Organic Frameworks - chemistry</topic><topic>Phosphates - chemistry</topic><topic>Phthalic Acids</topic><topic>Silicon Dioxide - chemistry</topic><topic>Solid Phase Extraction - methods</topic><topic>Sugar phosphates</topic><topic>Zirconium - chemistry</topic><topic>ZrO2</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hu, Yuyan</creatorcontrib><creatorcontrib>Wu, Boxue</creatorcontrib><creatorcontrib>Tang, Ying-Shu</creatorcontrib><creatorcontrib>Wu, Yi</creatorcontrib><creatorcontrib>Liu, Li-Yan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hu, Yuyan</au><au>Wu, Boxue</au><au>Tang, Ying-Shu</au><au>Wu, Yi</au><au>Liu, Li-Yan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dispersive solid-phase extraction based on zirconium metal-organic framework coupled with gas chromatography-mass spectrometry for determining sugar phosphates in biological samples</atitle><jtitle>Analytica chimica acta</jtitle><addtitle>Anal Chim Acta</addtitle><date>2024-08-15</date><risdate>2024</risdate><volume>1317</volume><spage>342908</spage><pages>342908-</pages><artnum>342908</artnum><issn>0003-2670</issn><issn>1873-4324</issn><eissn>1873-4324</eissn><abstract>Sugar phosphates (SPx) play important role in the metabolism of the organism. SPx such as glycerate 3-phosphate, fructose 6-phosphate and glucose 6-phosphate in biological samples have the poor stability, similar structure and low abundance, which make their separation and detection more challenging.
UiO-66-NH2 and ZrO2 coated SiO2(SBA-15) hard-core–shell adsorbents (UiO-66-NH2@SBA-15 and ZrO2@SBA-15) were synthesized, which were further used for dispersive solid-phase extraction for enriching the SPx in biological samples. The protocol was developed by UiO-66-NH2@SBA-15 and ZrO2@SBA-15 coupled with gas chromatography-mass spectrometry for the detection of trace SPx. The univariate experiment and response surface methodology were used to optimize the adsorption and desorption conditions.
The adsorbents showed excellent adsorption capacity and specificity towards SPx, which were proved by adsorption and selective experiments. Under the optimized conditions, there were good linearity within the range of 5.0–5000.0 ng mL−1, low limits of detection (0.001–1.0 ng mL−1), low limits of quantification (0.005–5.0 ng mL−1) and good precision (relative standard deviation less than 14.7 % for intra-day and inter-day). The satisfactory recoveries (89.1–113.8 %) and precision (0.5–14.6 %) were obtained when the sorbents were used to extract SPx from serum, saliva and cell samples. Moreover, UiO-66-NH2@SBA-15 was applied to the quantitative analysis of SPx from gastric cancer patients, because of a higher adsorption capacity (169.5–196.1 mg g−1).
UiO-66-NH2@SBA-15 showed great potential in the extraction of SPx in biological samples, which was beneficial to find out the metabolic change of SPx and explain the pathogenesis of the disease.
[Display omitted]
•UiO-66-NH2@SBA-15 and ZrO2@SBA-15 demonstrated specific adsorption towards sugar phosphates.•An efficient dispersive solid phase extraction protocol was developed for sugar phosphates in the biological samples.•UiO-66-NH2@SBA-15 exhibited the highest adsorption capacity.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>39030009</pmid><doi>10.1016/j.aca.2024.342908</doi><orcidid>https://orcid.org/0000-0001-6766-9508</orcidid></addata></record> |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Adsorption Biological samples Dispersive solid-phase extraction Gas Chromatography-Mass Spectrometry - methods Humans Limit of Detection Metal-organic frameworks Metal-Organic Frameworks - chemistry Phosphates - chemistry Phthalic Acids Silicon Dioxide - chemistry Solid Phase Extraction - methods Sugar phosphates Zirconium - chemistry ZrO2 |
| title | Dispersive solid-phase extraction based on zirconium metal-organic framework coupled with gas chromatography-mass spectrometry for determining sugar phosphates in biological samples |
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