Unraveling the influence of CRISPR/Cas13a reaction components on enhancing trans-cleavage activity for ultrasensitive on-chip RNA detection

The CRISPR/Cas13 nucleases have been widely documented for nucleic acid detection. Understanding the intricacies of CRISPR/Cas13’s reaction components is pivotal for harnessing its full potential for biosensing applications. Herein, we report on the influence of CRISPR/Cas13a reaction components on...

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Veröffentlicht in:	Mikrochimica acta (1966) 2024-08, Vol.191 (8), p.466, Article 466
Hauptverfasser:	He, Qian, Chen, Qun, Lian, Lijin, Qu, Jiuxin, Yuan, Xi, Wang, Chuhui, Xu, Lidan, Wei, Jiazhang, Zeng, Shaoling, Yu, Dongmei, Dong, Yuhan, Zhang, Yongbing, Deng, Lin, Du, Ke, Zhang, Canyang, Pandey, Vijay, Gul, Ijaz, Qin, Peiwu
Format:	Artikel
Sprache:	eng
Schlagworte:	Amplification Analytical Chemistry Biosensing Techniques - methods Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Cleavage COVID-19 - diagnosis COVID-19 - virology COVID-19 Nucleic Acid Testing - methods CRISPR CRISPR-Associated Proteins CRISPR-Cas Systems Humans Lab-On-A-Chip Devices Limit of Detection Magnesium - chemistry Microengineering Microscopy, Fluorescence Nanochemistry Nanotechnology Nucleic acids Original Paper Ribonucleic acid RNA RNA, Viral - analysis RNA, Viral - genetics SARS-CoV-2 - genetics Sensitivity Severe acute respiratory syndrome coronavirus 2 Target detection
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