Biological and Physicochemical Analysis of Sr-Doped Hydroxyapatite/Chitosan Composite Layers
In this work results are presented on the evaluation of HAp, HApSr, HAp_CS, and HApSr_CS layers deposited on Ti substrates regarding L929 cell viability and cytotoxicity as well as antimicrobial activity against , in connection with their physicochemical properties. The HAp and HApSr layers generate...
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| Veröffentlicht in: | Polymers 2024-07, Vol.16 (13), p.1922 |
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| creator | Zarif, Maria Elena Bita, Bogdan Yehia-Alexe, Sasa Alexandra Negut, Irina Gradisteanu Pircalabioru, Gratiela Andronescu, Ecaterina Groza, Andreea |
| description | In this work results are presented on the evaluation of HAp, HApSr, HAp_CS, and HApSr_CS layers deposited on Ti substrates regarding L929 cell viability and cytotoxicity as well as antimicrobial activity against
, in connection with their physicochemical properties. The HAp and HApSr layers generated by radio-frequency magnetron sputtering technique were further covered with chitosan by a matrix-assisted pulsed laser evaporation technique. During the plasma depositions, the Ti substrates were heated externally by a home-made oven above 100 °C. The HApSr_CS layers generated on the unpolished Ti substrates at 100 °C and 400 °C showed the highest biocompatibility properties and antimicrobial activity against
. The morphology of the layer surfaces, revealed by scanning electron microscopy, is dependent on substrate temperature and substrate surface roughness. The optically polished surfaces of Ti substrates revealed grain-like and microchannel structure morphologies of the layers deposited at 25 °C substrate temperature and 400 °C, respectively. Chitosan has no major influence on HAp and HApSr layer surface morphologies. X-ray photoelectron spectroscopy indicated the presence of Ca 2p
peak characteristic of the HAp structure even in the case of the HApSr_CS samples generated at a 400 °C substrate temperature. Fourier transform infrared spectroscopy investigations showed shifts in the wavenumber positions of the P-O absorption bands as a function of Sr or chitosan presence in the HAp layers generated at 25, 100, and 400 °C substrate temperatures. |
| doi_str_mv | 10.3390/polym16131922 |
| format | Article |
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, in connection with their physicochemical properties. The HAp and HApSr layers generated by radio-frequency magnetron sputtering technique were further covered with chitosan by a matrix-assisted pulsed laser evaporation technique. During the plasma depositions, the Ti substrates were heated externally by a home-made oven above 100 °C. The HApSr_CS layers generated on the unpolished Ti substrates at 100 °C and 400 °C showed the highest biocompatibility properties and antimicrobial activity against
. The morphology of the layer surfaces, revealed by scanning electron microscopy, is dependent on substrate temperature and substrate surface roughness. The optically polished surfaces of Ti substrates revealed grain-like and microchannel structure morphologies of the layers deposited at 25 °C substrate temperature and 400 °C, respectively. Chitosan has no major influence on HAp and HApSr layer surface morphologies. X-ray photoelectron spectroscopy indicated the presence of Ca 2p
peak characteristic of the HAp structure even in the case of the HApSr_CS samples generated at a 400 °C substrate temperature. Fourier transform infrared spectroscopy investigations showed shifts in the wavenumber positions of the P-O absorption bands as a function of Sr or chitosan presence in the HAp layers generated at 25, 100, and 400 °C substrate temperatures.</description><identifier>ISSN: 2073-4360</identifier><identifier>EISSN: 2073-4360</identifier><identifier>DOI: 10.3390/polym16131922</identifier><identifier>PMID: 39000777</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Absorption spectra ; Antimicrobial agents ; Banded structure ; Biocompatibility ; Biological effects ; Bones ; Chitosan ; Composite materials ; Fibroblasts ; Fourier transforms ; Hydroxyapatite ; Infrared spectroscopy ; Laser beam heating ; Lasers ; Magnetic properties ; Magnetron sputtering ; Microchannels ; Morphology ; Nitrates ; Photoelectrons ; Plasma ; Pulsed lasers ; Strontium ; Substrates ; Surface roughness ; Temperature dependence ; Tissue engineering ; Wavelengths ; X ray photoelectron spectroscopy</subject><ispartof>Polymers, 2024-07, Vol.16 (13), p.1922</ispartof><rights>2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c246t-163c380cb9438300f0741baa030dbcddb9816d05a5ad2fe68ff3c43c969d01fc3</cites><orcidid>0000-0002-4102-635X ; 0000-0003-4038-7548 ; 0000-0002-2226-5849 ; 0000-0002-4481-6744 ; 0000-0002-8141-5771</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39000777$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zarif, Maria Elena</creatorcontrib><creatorcontrib>Bita, Bogdan</creatorcontrib><creatorcontrib>Yehia-Alexe, Sasa Alexandra</creatorcontrib><creatorcontrib>Negut, Irina</creatorcontrib><creatorcontrib>Gradisteanu Pircalabioru, Gratiela</creatorcontrib><creatorcontrib>Andronescu, Ecaterina</creatorcontrib><creatorcontrib>Groza, Andreea</creatorcontrib><title>Biological and Physicochemical Analysis of Sr-Doped Hydroxyapatite/Chitosan Composite Layers</title><title>Polymers</title><addtitle>Polymers (Basel)</addtitle><description>In this work results are presented on the evaluation of HAp, HApSr, HAp_CS, and HApSr_CS layers deposited on Ti substrates regarding L929 cell viability and cytotoxicity as well as antimicrobial activity against
, in connection with their physicochemical properties. The HAp and HApSr layers generated by radio-frequency magnetron sputtering technique were further covered with chitosan by a matrix-assisted pulsed laser evaporation technique. During the plasma depositions, the Ti substrates were heated externally by a home-made oven above 100 °C. The HApSr_CS layers generated on the unpolished Ti substrates at 100 °C and 400 °C showed the highest biocompatibility properties and antimicrobial activity against
. The morphology of the layer surfaces, revealed by scanning electron microscopy, is dependent on substrate temperature and substrate surface roughness. The optically polished surfaces of Ti substrates revealed grain-like and microchannel structure morphologies of the layers deposited at 25 °C substrate temperature and 400 °C, respectively. Chitosan has no major influence on HAp and HApSr layer surface morphologies. X-ray photoelectron spectroscopy indicated the presence of Ca 2p
peak characteristic of the HAp structure even in the case of the HApSr_CS samples generated at a 400 °C substrate temperature. Fourier transform infrared spectroscopy investigations showed shifts in the wavenumber positions of the P-O absorption bands as a function of Sr or chitosan presence in the HAp layers generated at 25, 100, and 400 °C substrate temperatures.</description><subject>Absorption spectra</subject><subject>Antimicrobial agents</subject><subject>Banded structure</subject><subject>Biocompatibility</subject><subject>Biological effects</subject><subject>Bones</subject><subject>Chitosan</subject><subject>Composite materials</subject><subject>Fibroblasts</subject><subject>Fourier transforms</subject><subject>Hydroxyapatite</subject><subject>Infrared spectroscopy</subject><subject>Laser beam heating</subject><subject>Lasers</subject><subject>Magnetic properties</subject><subject>Magnetron sputtering</subject><subject>Microchannels</subject><subject>Morphology</subject><subject>Nitrates</subject><subject>Photoelectrons</subject><subject>Plasma</subject><subject>Pulsed lasers</subject><subject>Strontium</subject><subject>Substrates</subject><subject>Surface roughness</subject><subject>Temperature dependence</subject><subject>Tissue engineering</subject><subject>Wavelengths</subject><subject>X ray photoelectron spectroscopy</subject><issn>2073-4360</issn><issn>2073-4360</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpdkM1Lw0AQxRdRbKk9epWAFy_R2cx2kxxr_ahQUFBvQtjsbmxKko27CZj_3tVWUecyw-M3D94j5JjCOWIKF62phppyijSNoj0yjiDGkCGH_V_3iEyd24AfNuOcxodk5H8B4jgek5fL0lTmtZSiCkSjgof14Epp5FrXX9q8EZVXXGCK4NGGV6bVKlgOypr3QbSiKzt9sViXnXGiCRambo3zUrASg7buiBwUonJ6utsT8nxz_bRYhqv727vFfBXKiPEupBwlJiDzlGGCAAXEjOZCAILKpVJ5mlCuYCZmQkWF5klRoGQoU54qoIXECTnb-rbWvPXadVldOqmrSjTa9C5DiNOEA1Lm0dN_6Mb01ofcUhQSZNxT4ZaS1jhndZG1tqyFHTIK2Wfz2Z_mPX-yc-3zWqsf-rtn_ABHNX8_</recordid><startdate>20240705</startdate><enddate>20240705</enddate><creator>Zarif, Maria Elena</creator><creator>Bita, Bogdan</creator><creator>Yehia-Alexe, Sasa Alexandra</creator><creator>Negut, Irina</creator><creator>Gradisteanu Pircalabioru, Gratiela</creator><creator>Andronescu, Ecaterina</creator><creator>Groza, Andreea</creator><general>MDPI AG</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>KB.</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-4102-635X</orcidid><orcidid>https://orcid.org/0000-0003-4038-7548</orcidid><orcidid>https://orcid.org/0000-0002-2226-5849</orcidid><orcidid>https://orcid.org/0000-0002-4481-6744</orcidid><orcidid>https://orcid.org/0000-0002-8141-5771</orcidid></search><sort><creationdate>20240705</creationdate><title>Biological and Physicochemical Analysis of Sr-Doped Hydroxyapatite/Chitosan Composite Layers</title><author>Zarif, Maria Elena ; Bita, Bogdan ; Yehia-Alexe, Sasa Alexandra ; Negut, Irina ; Gradisteanu Pircalabioru, Gratiela ; Andronescu, Ecaterina ; Groza, Andreea</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c246t-163c380cb9438300f0741baa030dbcddb9816d05a5ad2fe68ff3c43c969d01fc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Absorption spectra</topic><topic>Antimicrobial agents</topic><topic>Banded structure</topic><topic>Biocompatibility</topic><topic>Biological effects</topic><topic>Bones</topic><topic>Chitosan</topic><topic>Composite materials</topic><topic>Fibroblasts</topic><topic>Fourier transforms</topic><topic>Hydroxyapatite</topic><topic>Infrared spectroscopy</topic><topic>Laser beam heating</topic><topic>Lasers</topic><topic>Magnetic properties</topic><topic>Magnetron sputtering</topic><topic>Microchannels</topic><topic>Morphology</topic><topic>Nitrates</topic><topic>Photoelectrons</topic><topic>Plasma</topic><topic>Pulsed lasers</topic><topic>Strontium</topic><topic>Substrates</topic><topic>Surface roughness</topic><topic>Temperature dependence</topic><topic>Tissue engineering</topic><topic>Wavelengths</topic><topic>X ray photoelectron spectroscopy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zarif, Maria Elena</creatorcontrib><creatorcontrib>Bita, Bogdan</creatorcontrib><creatorcontrib>Yehia-Alexe, Sasa Alexandra</creatorcontrib><creatorcontrib>Negut, Irina</creatorcontrib><creatorcontrib>Gradisteanu Pircalabioru, Gratiela</creatorcontrib><creatorcontrib>Andronescu, Ecaterina</creatorcontrib><creatorcontrib>Groza, Andreea</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>Materials Science Database</collection><collection>Materials Science Collection</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><jtitle>Polymers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zarif, Maria Elena</au><au>Bita, Bogdan</au><au>Yehia-Alexe, Sasa Alexandra</au><au>Negut, Irina</au><au>Gradisteanu Pircalabioru, Gratiela</au><au>Andronescu, Ecaterina</au><au>Groza, Andreea</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biological and Physicochemical Analysis of Sr-Doped Hydroxyapatite/Chitosan Composite Layers</atitle><jtitle>Polymers</jtitle><addtitle>Polymers (Basel)</addtitle><date>2024-07-05</date><risdate>2024</risdate><volume>16</volume><issue>13</issue><spage>1922</spage><pages>1922-</pages><issn>2073-4360</issn><eissn>2073-4360</eissn><abstract>In this work results are presented on the evaluation of HAp, HApSr, HAp_CS, and HApSr_CS layers deposited on Ti substrates regarding L929 cell viability and cytotoxicity as well as antimicrobial activity against
, in connection with their physicochemical properties. The HAp and HApSr layers generated by radio-frequency magnetron sputtering technique were further covered with chitosan by a matrix-assisted pulsed laser evaporation technique. During the plasma depositions, the Ti substrates were heated externally by a home-made oven above 100 °C. The HApSr_CS layers generated on the unpolished Ti substrates at 100 °C and 400 °C showed the highest biocompatibility properties and antimicrobial activity against
. The morphology of the layer surfaces, revealed by scanning electron microscopy, is dependent on substrate temperature and substrate surface roughness. The optically polished surfaces of Ti substrates revealed grain-like and microchannel structure morphologies of the layers deposited at 25 °C substrate temperature and 400 °C, respectively. Chitosan has no major influence on HAp and HApSr layer surface morphologies. X-ray photoelectron spectroscopy indicated the presence of Ca 2p
peak characteristic of the HAp structure even in the case of the HApSr_CS samples generated at a 400 °C substrate temperature. Fourier transform infrared spectroscopy investigations showed shifts in the wavenumber positions of the P-O absorption bands as a function of Sr or chitosan presence in the HAp layers generated at 25, 100, and 400 °C substrate temperatures.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>39000777</pmid><doi>10.3390/polym16131922</doi><orcidid>https://orcid.org/0000-0002-4102-635X</orcidid><orcidid>https://orcid.org/0000-0003-4038-7548</orcidid><orcidid>https://orcid.org/0000-0002-2226-5849</orcidid><orcidid>https://orcid.org/0000-0002-4481-6744</orcidid><orcidid>https://orcid.org/0000-0002-8141-5771</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Absorption spectra Antimicrobial agents Banded structure Biocompatibility Biological effects Bones Chitosan Composite materials Fibroblasts Fourier transforms Hydroxyapatite Infrared spectroscopy Laser beam heating Lasers Magnetic properties Magnetron sputtering Microchannels Morphology Nitrates Photoelectrons Plasma Pulsed lasers Strontium Substrates Surface roughness Temperature dependence Tissue engineering Wavelengths X ray photoelectron spectroscopy |
| title | Biological and Physicochemical Analysis of Sr-Doped Hydroxyapatite/Chitosan Composite Layers |
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