Comparative single-cell transcriptional and proteomic atlas of clinical-grade injectable mesenchymal source tissues
Bone marrow aspirate concentrate (BMAC) and adipose-derived stromal vascular fraction (ADSVF) are the most marketed stem cell therapies to treat a variety of conditions in the general population and elite athletes. Both tissues have been used interchangeably clinically even though their detailed com...
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| Veröffentlicht in: | Science advances 2024-07, Vol.10 (28), p.eadn2831 |
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| creator | Ruoss, Severin Nasamran, Chanond A Ball, Scott T Chen, Jeffrey L Halter, Kenneth N Bruno, Kelly A Whisenant, Thomas C Parekh, Jesal N Dorn, Shanelle N Esparza, Mary C Bremner, Shannon N Fisch, Kathleen M Engler, Adam J Ward, Samuel R |
| description | Bone marrow aspirate concentrate (BMAC) and adipose-derived stromal vascular fraction (ADSVF) are the most marketed stem cell therapies to treat a variety of conditions in the general population and elite athletes. Both tissues have been used interchangeably clinically even though their detailed composition, heterogeneity, and mechanisms of action have neither been rigorously inventoried nor compared. This lack of information has prevented investigations into ideal dosages and has facilitated anecdata and misinformation. Here, we analyzed single-cell transcriptomes, proteomes, and flow cytometry profiles from paired clinical-grade BMAC and ADSVF. This comparative transcriptional atlas challenges the prevalent notion that there is one therapeutic cell type present in both tissues. We also provide data of surface markers that may enable isolation and investigation of cell (sub)populations. Furthermore, the proteome atlas highlights intertissue and interpatient heterogeneity of injected proteins with potentially regenerative or immunomodulatory capacities. An interactive webtool is available online. |
| doi_str_mv | 10.1126/sciadv.adn2831 |
| format | Article |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Adipose Tissue - metabolism Bone Marrow Cells - cytology Bone Marrow Cells - metabolism Gene Expression Profiling Humans Mesenchymal Stem Cells - cytology Mesenchymal Stem Cells - metabolism Proteome - metabolism Proteomics - methods Single-Cell Analysis - methods Transcriptome |
| title | Comparative single-cell transcriptional and proteomic atlas of clinical-grade injectable mesenchymal source tissues |
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